SustainPineDB

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Assembly info
Unigenes
SNPs
SSRs
Descriptions
GOs
ECs
KEGGs
InterPros
miRNAs

Assembly Name: uagpf v2.0

UniGene Name: uagpf_v2_unigene25532

Length: 561 nt

UniGene Fasta

>uagpf_v2_unigene25532
CGTCGTGACCTTGGCTGTCACTCATTTATGACCTCAAATTTGAAATTGTCTTTGTTTTGGCTGTCACTGCTGTGGATGCCATATTTGTCTACACAAAACAAGGACACATGGCTTCCCTTTTGTCAAGTTGTCGTCCAGACTGTCCAATTTTTGCCTTTACTACCTCAACGACTTTAAGGCGACGATTGAATCTTCAATGGGGTCTCATTCCTTTTAGACTGAATTTCTCAGAGGATATGGAGAGTAACCTTTACCGTACCTTTGCTTTGCTGAAAGCAAGAGGTATGGTGAAATCAGGTGACTTGGTAATTGCTGTTTCAGATTTTCTGCAGTCCATTCAGGTAATCAATGTGCCATAATGACATTTTCGGGGTTTCTGATTGTTGCTTCACAATTAACTTGGGCGCTTGTGTTACTAATCAAGAAGCAAGCATTTTCTCTGAAGGAAATGTTGGTGTTTGATCCCAATTTATTCTCCAGAGTGTTAAAGAGCCAGATTAGCTTATTCAAATCAGTTTATTTGCCTGAATTGCAAATAATGATGTAATTAAATCTGCTACA

Ace file of the UniGene uagpf_v2_unigene25532

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Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene9477	7/8

Source	Descriptions	Term	Type	Identity
AutoFact	Pyruvate kinase n=2 Tax=Populus trichocarpa RepID=B9HKC4_POPTR	-	-	79%
FL-Next	sp=Pyruvate kinase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	37%
Blast2go	pyruvate kinase	-	-	89%

Source	ECs	Term	Type	e value	Identity
Blast2go		EC:2.7.1.4-	-	0.0	89%

Source	GOs	Term	Type	Identity
Blast2go	lipid metabolic process	GO:0006629	Biological Process	89%
Blast2go	glycolysis	GO:0006096	Biological Process	89%
Blast2go	phosphorylation	GO:0016310	Biological Process	89%
Blast2go	response to cadmium ion	GO:0046686	Biological Process	89%
Blast2go	potassium ion binding	GO:0030955	Molecular Function	89%
Blast2go	ATP binding	GO:0005524	Molecular Function	89%
Blast2go	pyruvate kinase activity	GO:0004743	Molecular Function	89%
Blast2go	magnesium ion binding	GO:0000287	Molecular Function	89%
Blast2go	chloroplast stroma	GO:0009570	Cellular Component	89%

Source	InterPros	Term	Type	Identity
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Serine-threonine/tyrosine-protein kinase catalytic domain	IPR001245	-	-
Sma3	Pyruvate kinase	IPR001697	-	-
Sma3	Tetratricopeptide-like helical	IPR011990	-	-
Sma3	Pyruvate kinase, barrel	IPR015793	-	-
Sma3	Pyruvate kinase, alpha/beta	IPR015794	-	-
Sma3	Pyruvate kinase, beta-barrel insert domain	IPR015806	-	-
Sma3	Pyruvate/Phosphoenolpyruvate kinase	IPR015813	-	-
Sma3	Pyruvate kinase, active site	IPR018209	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LL33

Fln msg: Unexpected stop codon in the beginning of your sequence, your sequence is shorter than subject: 119 - 591

Fln protein: S*PWLSLIYDLKFEIVFVLAVTAVDAIFVYTKQGHMASLLSSCRPDCPIFAFTTSTTLRRRLNLQWGLIPFRLNFSEDMESNLYRTFALLKARGMVKSGDLVIAVSDFLQSIQVINVP*

Protein Length: 120

Fln nts: CGTCGTGACCTTGGCTGTCACTCATTTATGACCTCAAATTTGAAATTGTCTTTGTTTTGGCTGTCACTGCTGTGGATGCCATATTTGTCTACACAAAACAAGGACACATGGCTTCCCTTTTGTCAAGTTGTCGTCCAGACTGTCCAATTTTTGCCTTTACTACCTCAACGACTTTAAGGCGACGATTGAATCTTCAATGGGGTCTCATTCCTTTTAGACTGAATTTCTCAGAGGATATGGAGAGTAACCTTTACCGTACCTTTGCTTTGCTGAAAGCAAGAGGTATGGTGAAATCAGGTGACTTGGTAATTGCTGTTTCAGATTTTCTGCAGTCCATTCAGGTAATCAATGTGCCATAA___TGACATTTTCGGGGTTTCTGATTGTTGCTTCACAATTAACTTGGGCGCTTGTGTTACTAATCAAGAAGCAAGCATTTTCTCTGAAGGAAATGTTGGTGTTTGATCCCAATTTATTCTCCAGAGTGTTAAAGAGCCAGATTAGCTTATTCAAATCAGTTTATTTGCCTGAATTGCAAATAATGATGTAATTAAATCTGCTACA

Fln Alignment:

uagpf_mira_c2030___VLAVTAVDAIFVYTKQGHMASLLSSCRPDCPIFAFXXXXXXXXXXXXQWGLIPFRLNFSEDMESNLYRTFALLKARGMVKSGDLVIAVSDFLQSI
B8LL33________________MMSNTLGTSIVVFTRTGFMATLLSHYRPFGTIFAFTNQERVKQRLALYRGIRPIYMPFSNDAEETFSKALSLLQKLGMLKEGEQVALVQSGRQPI

SNPs (tot: 30)

Position	A %	C %	G %	T %	Probability
104	66			33	0.997459
105	66	33			0.997459
136	66	33			1.0
137		40	60		1.0
139		40		60	1.0
140	20		80		0.995493
141			20	80	0.995493
145	16			83	0.994514
147	20	80			0.995493
148		20		20	0.99255
149	60			40	1.0
150		20	80		0.995493
151	80		20		0.995493
159	80	20			0.995493
162	16			83	0.994523
183			60	40	1.0
202	75	12			0.975346
203	87		12		0.992557
204		14		85	0.993533
206			12	87	0.992557
208		71	28		1.0
209	85		14		0.993533
211	71	28			1.0
213			75	12	0.975346
215			85	14	0.993533
216		25	75		1.0
217	14			85	0.993533
218		85	14		0.993533
222		14		85	0.993533
225	14	85			0.993533

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain