SustainPineDB

Assembly Name: uagpf v2.0

UniGene Name: uagpf_v2_unigene18614

Length: 1095 nt

UniGene Fasta

Ace file of the UniGene uagpf_v2_unigene18614

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene7725	26/28

Source	Descriptions	Term	Type	Identity
AutoFact	putative enoyl-CoA hydratase [Oryza sativa]	-	-	54%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	28%
Blast2go	methylglutaconyl- mitochondrial	-	-	80%

Source	ECs	Term	Type	e value	Identity
Blast2go	Enoyl-CoA hydratase.	EC:4.2.1.17	-	0.0	80%

Source	KEGGs	Term	Type	Identity
Blast2go	Fatty acid elongation in mitochondria	00062		80%
Blast2go	Fatty acid metabolism	00071		80%
Blast2go	Valine, leucine and isoleucine degradation	00280		80%
Blast2go	Geraniol degradation	00281		80%
Blast2go	Lysine degradation	00310		80%
Blast2go	Benzoate degradation	00362		80%
Blast2go	Tryptophan metabolism	00380		80%
Blast2go	beta-Alanine metabolism	00410		80%
Blast2go	alpha-Linolenic acid metabolism	00592		80%
Blast2go	Aminobenzoate degradation	00627		80%
Blast2go	Propanoate metabolism	00640		80%
Blast2go	Butanoate metabolism	00650		80%
Blast2go	Limonene and pinene degradation	00903		80%
Blast2go	Biosynthesis of unsaturated fatty acids	01040		80%
Blast2go	Biosynthesis of plant hormones	01070		80%
Blast2go	Metabolic pathways	01100		80%
Blast2go	Biosynthesis of secondary metabolites	01110		80%
Blast2go	Methylglutaconyl-CoA hydratase.	EC:4.2.1.18	-	80%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Valine, leucine and isoleucine degradation	00280		0.0	80%
Blast2go	Metabolic pathways	01100		0.0	80%

Source	GOs	Term	Type	Identity
Blast2go	fatty acid metabolic process	GO:0006631	Biological Process	80%
Blast2go	enoyl-CoA hydratase activity	GO:0004300	Molecular Function	80%
Blast2go	methylglutaconyl-CoA hydratase activity	GO:0004490	Molecular Function	80%
Blast2go	mitochondrion	GO:0005739	Cellular Component	80%

Source	InterPros	Term	Type	e value	Identity
Sma3	Crotonase, core	IPR001753	-	0.0	-
Sma3	Enoyl-CoA hydratase/isomerase, conserved site	IPR018376	-	0.0	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: A9NUY4

Fln msg: atg_distance in limit (1-15): atg_distance = 10, W2: There is no M at the beginning, your sequence is shorter than subject: 255 - 273

Fln protein: RRDLGCHSIGVTLDRPGAMNAIGTDTLKYLQRIIETLNEDSSARVVILCSSLPKVFCAGADLKDAEG*MFLKSKTLSALCDQPSHF*RHFLYLQLL*LREQRYVVTLAVTHSCDLRVCGDAATFGMPETGLAIIPGAGGTQRLPRLIGRSQAKELILTGRKVDGKQALSLGLVNHCVAAGDAYSKALEIARDINEKGPLAIKMAKFSIDRGSEVDTYSGMFVEESCYAELLNSNDRLEGLAAFAEKRKPNYTGE*

Protein Length: 256

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTGGGGTGACTTTAGATAGGCCTGGGGCCATGAATGCTATAGGGACTGATACTCTCAAATATTTGCAACGTATCATCGAAACACTAAATGAAGACTCTTCTGCTCGTGTAGTTATATTGTGCAGTTCTTTACCTAAAGTATTTTGTGCAGGCGCTGATCTGAAGGACGCCGAAGGATGAATGTTTCTGAAGTCGAAAACTTTGTCAGCTCTTTGCGATCAACCTTCTCATTTTTAGAGGCACTTTCTGTACCTACAATTGCTGTGATTGAGGGAGCAGCGCTACGTCGTGACCTTGGCTGTCACTCATTCTTGTGACCTACGTGTCTGTGGAGATGCGGCAACCTTTGGAATGCCAGAGACAGGACTTGCTATCATTCCTGGTGCAGGTGGAACCCAGCGTCTTCCAAGATTGATAGGGAGGTCACAGGCAAAAGAGCTTATCTTGACAGGCCGCAAGGTTGATGGGAAACAAGCGTTGTCCCTGGGTCTTGTAAACCATTGTGTAGCAGCAGGAGATGCATATTCAAAGGCTCTTGAGATTGCTCGAGATATCAACGAGAAGGGTCCTTTGGCTATTAAAATGGCCAAATTTTCCATTGATCGAGGTAGTGAGGTCGACACATATTCAGGAATGTTTGTTGAGGAATCTTGCTATGCAGAGCTCTTAAATTCAAATGACCGATTGGAAGGCTTGGCAGCATTTGCTGAGAAGCGGAAGCCAAACTACACTGGTGAATAG___ATTGTGGATGAGCTGGTGTATTTTCCATCAATTTCAAACATTTCTGTGCCCTGAGTTACAAATTTGTTAAACTCTGAATGGAGGGATCATTGTAACTACCTTCCAATCATGAAAATTCATTCTTATCTAAACAGTGAACGTGATATTGACCTTCCACTAATGGTAGTTCATTCTTATCTAAAAAATGATTGTGATAGTTGACCATATGATACCATAATGGTTTGTGAGAATAGCATTAAGCTGAAAATGATCATATTAGAGCTGTGGACATGATATAAAAATTAAGAAATAATCTTTGAATAGCAAATTGGACATGCTTTCTGACAAAAC

Fln Alignment:

Contig18614___IGVTLDRPGAMNAIGTDTLKYLQRIIETLNEDSSARVVILCSSLPKVFCAGADLKDAEG*MFLKSKTLSALCDQPSHF*RHFXXXXXXXXXXXXXXXTLAVTH------------SCDLRVCGDAATFGMPETGLAIIPGAGGTQRLPRLIGRSQAKELILTGRKVDGKQALSLGLVNHCVAA-GDAYSKALEIARDINEKGPLAIKMAKFSIDRGSEVDTYSGMFVEESCYAELLNSNDRLEGLAAFAEKRKPNYT
A9NUY4________________VQLILNRPSQRNALDDHFFTEFPRLLSALDENPSVRIVILKSS-GNHFCSGIDLSVLSSVVGSQGEDQGRVREKLRRNIKRMQAAFSAVEECRKP--VIAAVHGACIGAAVDLITACDIRYCSQDAFFSVKEVDLAITADLGTLQRLPRLVGDGNAWDLALTGRRIDALEAKQMGLVQKVYSTKSDMDENVGKIARDIASKSPLAVIGTKTVLMNSRDLSVAKGLDYVATWNAATLSSTDVNEVFTAQVEKRKPMFS

SNPs (tot: 56)

Position	A %	C %	G %	T %	Probability
310		66	33		0.997452
312		33		66	0.997452
313		66	33		0.997452
318		66	33		0.997452
319		66	33		0.997452
324	33	66			0.997452
325			33	66	0.997452
326			66	33	0.997452
328		66	33		0.997452
329	66		33		0.997452
333	66			33	0.997452
429			25	75	0.996473
433	75		25		0.996473
435		25	75		0.996473
436	75	25			0.996473
440	75		25		0.996473
441		25		75	0.996473
442	75			25	0.996473
444			75	25	0.996473
445		25	75		0.996473
448		33	66		0.997452
503		25		75	1.0
504		12		87	0.992554
507	12	87			0.992554
508	25	75			1.0
511	12	12	62	12	0.962769
512	12		87		0.992554
520	87	12			0.992554
522	87	12			0.992554
523		87		12	0.992554
525	88		11		0.923564
527		11		88	0.923564
533		11	88		0.923564
535	88			11	0.923564
537		88	11		0.923564
538	88	11			0.923564
539			88	11	0.923564
541	88			11	0.923564
542		11	88		0.923564
544		11		88	0.923564
545			88	11	0.923564
561			10	90	0.90298
565		10	90		0.90298
566	90	10			0.90298
574	72			27	1.0
575		11	88		0.999937
577		16		72	1.0
578	72	27			1.0
580		83		16	1.0
581	83		16		1.0
582	83		16		1.0
585	82			17	1.0
586		17	82		1.0
588	81			18	1.0
589		18	81		1.0
590	18		81		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain