SustainPineDB

Assembly Name: uagpf v2.0

UniGene Name: uagpf_v2_unigene13174

Length: 1914 nt

UniGene Fasta

Ace file of the UniGene uagpf_v2_unigene13174

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene25251	42/45
SustainPine v2.0	sp_v2.0_unigene81810	1/45

Source	Descriptions	Term	Type	Identity
AutoFact	long chain acyl-CoA synthetase 9 [Arabidopsis thaliana] sp\|Q9CAP8.1\|LACS9_ARATH RecName: Full=Long chain acyl-CoA synthetase 9, chloroplastic gb\|AAG51668.1\|AC010704_12 putative acyl-CoA synthetase; 62297-59022 [Arabidopsis thaliana] gb\|AAM28876.1\|AF503759	-	-	65%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	100%
Blast2go	annotation was added to scaffolds in november 2011~long-chain-fatty-acid- ligase	-	-	82%

Source	ECs	Term	Type	e value	Identity
Blast2go	Long-chain-fatty-acid--CoA ligase.	EC:6.2.1.3	-	0.0	82%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Fatty acid metabolism	00071		0.0	82%
Blast2go	Metabolic pathways	01100		0.0	82%

Source	GOs	Term	Type	Identity
Blast2go	long-chain fatty acid-CoA ligase activity	GO:0004467	Molecular Function	82%
Blast2go	chloroplast envelope	GO:0009941	Cellular Component	82%
Blast2go	membrane	GO:0016020	Cellular Component	82%
Blast2go	endoplasmic reticulum	GO:0005783	Cellular Component	82%

Source	InterPros	Term	Type	e value	Identity
Sma3	AMP-dependent synthetase/ligase	IPR000873	-	0.0	-
Sma3	Lipocalin	IPR002345	-	0.0	-

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: coniferopsida.fasta

Fln subject: D5ACR7

Fln msg: Distance to subject end: 154 aas, your sequence is shorter than subject: 152 - 197

Fln protein: MEGSWLGAWGPEKFLWDVLVFKKIRAVLGGHIRGLLSGGAPLSADTQRFINVCFGAPIGQGYGLTETCAGATFSEWDDMTVGRVGPPLPCSYVKLIDWEEAGYLTSDSPMPRGEIVIGGPHVTLGYFKNKEKTDESYKVDERGMRWFYTGDI

Protein Length: 153

Fln nts: CGTCGTGACCTTGGCTGTCACTCAGGTGTGGTATGCATTCTTAGGGTTTGTTTATCCTTCATTCACGTGTTCTGTGAATTCTCTGTAGAGGTATGTTAGCCTCCTTCACCTGGCCGATGAGTCCCGCTACTGAAGATCAGTTGCTTTCGATGAGCAAGGTTTTAAATTTTGCTTAAGTTTCTTAAGTTACTGGCCAGATGCAAGCAACCCAACTGTGCCCTTGTTGCAGTCATCATTAAAAGCAGATTCTCTGGCATCTGTCCAGCAATAAACCCTGTTCACAAAGATGAATGCCTACATTGTTGGCATCCTTGTGCCTGTGCTGCTATCATTCGTGTTAAGGAACAGAAGAAAGGGAAAGGTATATGGAGTGCCTGTGGATGTGGACGGGGATGGTGGTTTTGCAATCCGACACCATCGCAGTTCTTCCTTAGTTGAAACGCCGTGGGAAGGAGCTTCAACTCTTGCGGACCTCTTTGAGCAATCATGCAAAAGACATGCACAAATGCGCTTACTCGGAACCCGAAAGCTGATTAGCAGAGAAATGAAAGTCAGTGAAGATGGCAGGAGTTTTGAAAAGCTACATCTGGGAGATTATGAGTGGTTAAGTTATGGGCAAGTACTCGAGCGTGTTTGTAACTTTGCATCAGGTTTGGTAGCACTTGGTCATGAAAAAGGAGAGCGTGTTGCCATTTTTGCTGAAACTCGAGCAGAATGGTTCATTGCCTTGCAAGGTTGCTTCCGTCACAACAACACAGTAGTTACGATTTATGCTTCTTTGGGAGAGGAAGCTCTTGCTCACTCACTAAATGAGACAGAAGTGACAACAGTGATTTGTGATCGCAAGCAGTTAAAAAAGCTGATTGATACAAGCACACAATTGGATACTGTAAAGCATGTTATTTACATGGAAGACGAGGGCATTACATCCGAGCCATCTCTGTCTGGAGGGAGCAGCAGCTGGACAGTGTCATCTATGTCCAGGGTTGAGCGTTTGGGTCGGGAGAGTCCTGTTGATGCAGATTTGCCGGTAGCTGCTGACATTGCAGTTATAATGTATACAAGTGGCAGTACTGGCTTGCCTAAGGGAGTCATGATGACTCATGGAAATATTGTAGCGACAGTTGCTGCCGTGATGACAATTGTTCCAGGCCTTAGTAACAAAGACATATATCTGGCATACTTGCCGTTAGCTCATATTCTTGAACTAGAGGCTGAGGCTGTAATGGTTGCTTCAGGAATTGCTATTGGTTATGGTTCAACTTTGACACTAACTGATACATCAAGCAAGATTAAAAAAGGAACCAAAGGAGATGCTTCTATGTTGGGTCCAACATTGATGACTGCTGTACCGGCTATTTAGATCGTGTCAGGGATGGTGTGAGGAATAAGGTTGAGACAACTGGTGGAGTTGCAAAGAAATTGTTTAATATAGCATATAGACGTAGGTTGGCTGCT_-_ATGGAAGGAAGTTGGCTGGGGGCATGGGGTCCAGAGAAGTTTCTCTGGGATGTACTAGTTTTTAAAAAGATACGTGCAGTTCTTGGAGGCCATATCCGTGGTTTACTTTCTGGTGGGGCCCCTCTTTCAGCAGATACACAACGATTCATCAACGTTTGCTTTGGGGCACCAATTGGTCAGGGATATGGTCTCACTGAAACTTGTGCTGGTGCAACCTTTTCTGAATGGGATGATATGACTGTTGGTCGTGTTGGTCCTCCACTTCCCTGTTCCTATGTTAAGCTGATTGATTGGGAAGAAGCTGGGTATTTGACATCAGATTCACCAATGCCACGAGGAGAGATAGTTATTGGTGGTCCCCATGTAACTCTGGGCTATTTCAAGAACAAAGAGAAAACTGATGAGTCCTACAAGGTGGACGAGAGAGGAATGCGATGGTTCTATACTGGTGACATA

Fln Alignment:

Contig13174___MPRGEIVIGGPHVTLGYFKNKEKTDESYKVDERGMRWFYTGDI
D5ACR7________________MPRGEIVIGGPHVTLGYFKNKEKTDESYKVDERGMRWFYTGDI

SNPs (tot: 65)

Position	A %	C %	G %	T %	Probability
45		25	75		0.996473
48			25	75	0.996473
51		25		75	0.996473
52		25		75	0.996473
54	75			25	0.996473
55			25	75	0.996473
56		25	25	50	0.995533
62		25		75	0.996473
135			25	75	0.996473
206	50		50		1.0
686		25		75	0.996473
689		25		75	0.996473
691		75		25	0.996473
692		75	25		0.996473
694		16		83	0.994512
695		16		83	0.994512
698			16	83	0.994512
703	83			16	0.994512
704	83	16			0.994512
960	86		13		0.999973
961			86	13	0.999973
963			13	86	0.999973
964			86	13	0.999973
968	86	13			0.999973
969			86	13	0.999973
972			13	86	0.999973
976		86	13		0.999973
978	86	13			0.999973
979	13			86	0.999973
980		13	86		0.999973
983	14	85			0.999981
1003	23		76		1.0
1094		73		26	1.0
1130		21		78	1.0
1214	37		62		1.0
1241	83		16		0.994512
1382		25		75	0.996473
1385		25	75		0.996473
1386	75		25		0.996473
1387			75	25	0.996473
1390	75	25			0.996473
1391		25		75	0.996473
1392	75			25	0.996473
1393	75			25	0.996473
1396		25		75	0.996473
1399	75			25	0.996473
1400		25	75		0.996473
1404	75			25	0.996473
1407	25		75		0.996473
1560		25	75		0.996473
1564			25	75	0.996473
1567		25		75	0.996473
1571			25	75	0.996473
1573		25	75		0.996473
1576			75	25	0.996473
1577		25	75		0.996473
1578	25		75		0.996473
1580		75		25	0.996473
1582	25	75			0.996473
1598	60		40		1.0
1614	40		60		1.0
1616		50		50	1.0
1798	80			20	0.995493
1799		20	80		0.995493
1801	80	20			0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain