SustainPineDB

Assembly Name: uagpf v2.0

UniGene Name: uagpf_v2_unigene10480

Length: 1531 nt

UniGene Fasta

Ace file of the UniGene uagpf_v2_unigene10480

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene5898	33/33

Source	Descriptions	Term	Type	Identity
AutoFact	Acyl CoA synthetase, putative n=1 Tax=Ricinus communis RepID=B9RZF1_RICCO	-	-	58%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	47%
Blast2go	annotation was added to scaffolds in november 2011~long-chain-fatty-acid- ligase	-	-	83%

Source	ECs	Term	Type	e value	Identity
Blast2go	Long-chain-fatty-acid--CoA ligase.	EC:6.2.1.3	-	0.0	83%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Fatty acid metabolism	00071		0.0	83%
Blast2go	Metabolic pathways	01100		0.0	83%

Source	GOs	Term	Type	Identity
Blast2go	fatty acid biosynthetic process	GO:0006633	Biological Process	83%
Blast2go	long-chain fatty acid-CoA ligase activity	GO:0004467	Molecular Function	83%
Blast2go	plasma membrane	GO:0005886	Cellular Component	83%

Source	InterPros	Term	Type	e value	Identity
Sma3	AMP-dependent synthetase/ligase	IPR000873	-	0.0	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5ACR7

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 61 aas, putative chimeric sequence, your sequence is longer than subject: 347 - 197

Fln protein: MVTGIGYFLKCQNEELTEKDVYLSFLPLAHIFDRVIEEFFISKSASIGFWRGDVKLLVEDIAELKPTVFCGVPRVFDRIYSGLNQKIAAGGFLNRKVFEFAYKYKLNNMRKGLKHEKASPLFDKIVFSKIKQGLGGRVRVMLSGAAPLAKHVEEFLRVVTCCHVVQGYGLTETCAGSFVSLPNVMSMIGTVGPPVPNIDVCLESVPEMGYDALSEVPRGEVCIRGKTLFAGYYKREDLTNEVLVDGWFHTGDIGEWQANGALKIIDRKKIYLSFHKENMLQWKISRMCMASVLLLIRFGFTEIASNLVWLLLLYLINEHLKVGPQIMDKLEILLRCAKIPRQKNTF*

Protein Length: 348

Fln nts: CGTCGTGACCTTGGCTGTCACTCATTTCT_-_ATGGTGACCGGAATTGGTTATTTTCTTAAATGTCAGAATGAAGAGCTCACGGAGAAGGATGTTTATCTCTCGTTTCTTCCTCTAGCACATATATTTGATCGGGTGATAGAGGAATTTTTCATATCAAAAAGTGCTTCAATAGGCTTCTGGCGCGGGGATGTGAAACTTTTGGTGGAGGATATTGCAGAACTAAAACCAACGGTCTTCTGTGGTGTCCCACGTGTCTTTGATCGCATATATTCAGGTTTAAACCAGAAGATCGCTGCTGGAGGATTTTTGAATAGAAAAGTGTTTGAATTTGCATACAAATACAAGCTCAATAATATGCGCAAAGGGTTGAAACATGAAAAAGCATCTCCACTTTTTGATAAAATTGTCTTCAGCAAGATTAAACAAGGTCTGGGAGGACGTGTCCGTGTAATGTTATCAGGAGCAGCTCCGCTTGCAAAACATGTGGAAGAGTTTCTACGAGTAGTCACTTGTTGCCATGTTGTACAGGGCTATGGTTTGACAGAAACTTGCGCGGGAAGTTTTGTTTCCCTTCCAAATGTAATGTCAATGATCGGGACTGTTGGTCCACCTGTTCCAAATATTGATGTCTGCCTTGAGTCTGTCCCTGAAATGGGATATGATGCACTTTCAGAAGTACCGCGTGGAGAGGTTTGTATAAGGGGCAAAACCTTATTTGCTGGTTATTACAAGCGTGAAGACCTTACAAATGAGGTTTTGGTTGATGGTTGGTTTCACACAGGTGACATTGGGGAGTGGCAGGCGAATGGAGCACTAAAAATCATTGATAGAAAAAAAATATATTTAAGCTTTCACAAGGAGAATATGTTGCAGTGGAAAATCTCGAGAATGTGTATGGCCAGTGTTCTGCTATTGATTCGATTTGGGTTTACGGAAATAGCTTCGAATCTTGTCTGGTTGCTGTTGTTATACCTAATCAACGAGCACTTGAAAGTTGGGCCACAGATCATGGACAAACTGGAGATTTTACTGCGTTGTGCCAAAATCCCAAGGCAAAAGAATACATTTTGA___ATGAACTTAATGCAGTTGCTAAAAGTAGCAAGTTAAAGGGTTTCGAGTGTGTAAGAGCTATCCACCTTGATCCTGTGCCATTTGATATGGAAAGAGACTTGATTACTCCCACATTTAAAATGAAACGGCCACAACTACTGAAATACTACAAGGATATTATTGAAGATTTATACAAAAGCCTCAAATGAGTTGCAGTGCACGCAAAGTGAGAAATCACAATAAATTAGAATATCCTTGTCTATATTGAATCTATTTATTTAATGGTGTCATGTACAAGGCCTGATTTATGTCCCATTAGAATGCAACGCTTCTATTATTTCCCTTGTAATCTTTTGCTTTGTTAAACGGGGCATATTATAGGGCTATGTATATGCTGATTTTCATAATAAAATTAGGAAAAGGCCATCTGGTTAATGATTGGCTACCAACTTATGCTATTCAATACATAGTTTCTTAATGTA

Fln Alignment:

Contig10480___VPRGEVCIRGKTLFAGYYKREDLTNEVL-VDG----WFHTGDIGEWQANGALKIIDRKK
D5ACR7________________MPRGEIVIGGPHVTLGYFKNKEKTDESYKVDERGMRWFYTGDIGRFHPDGCLEIIDRKK

SNPs (tot: 66)

Position	A %	C %	G %	T %	Probability
170	80	20			0.995493
173		20	80		0.995493
174	20		80		0.995493
628		33		66	0.997452
642	33		66		0.997452
643			33	33	0.997753
650	33	66			0.997452
752	75	25			0.996473
753	75		25		0.996473
757	75			25	0.996473
759	25		75		0.996473
760		25		75	0.996473
761		25		75	0.996473
766		25		75	0.996473
771		33	66		0.997452
772	33		66		0.997452
773		33		66	0.997452
775		33	66		0.997452
776	33		66		0.997452
903		11	88		0.923564
904	11		88		0.923564
905		88		11	0.923564
908			88	11	0.923564
911	11			77	0.968132
912		11	11	77	0.968132
916		88		11	0.923564
917		11		88	0.923564
919		11	11	77	0.968132
921		22	77		0.999999
923		11		88	0.923564
926	11		88		0.923564
1007		88	11		0.923564
1008	88			11	0.923564
1010	88			11	0.923564
1011		10	90		0.90298
1012	90		10		0.90298
1013		10		90	0.90298
1018	10		90		0.90298
1020		90	10		0.90298
1021	80			20	0.999996
1023	90		10		0.90298
1027		20	80		0.999996
1030	90	10			0.90298
1031	10			90	0.90298
1150		88	11		0.923564
1155	11	88			0.923564
1157	88	11			0.923564
1160			11	88	0.923564
1162	88	11			0.923564
1164	88		11		0.923564
1167		11	88		0.923564
1169	88	11			0.923564
1170	88			11	0.923564
1171		11	88		0.923564
1190	88	11			0.923564
1193		11		88	0.923564
1194	88		11		0.923564
1195	88	11			0.923564
1202	50		50		1.0
1247	90	10			0.90298
1249	90			10	0.90298
1252	90			10	0.90298
1253	90	10			0.90298
1278	40		60		1.0
1386		57		42	1.0
1413			60	40	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain