SustainPineDB

Assembly Name: uagpf v2.0

UniGene Name: uagpf_v2_unigene10091

Length: 906 nt

UniGene Fasta

Ace file of the UniGene uagpf_v2_unigene10091

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene7861	8/12
SustainPine v2.0	sp_v2.0_unigene16853	4/12

Source	Descriptions	Term	Type	Identity
AutoFact	Malic enzyme n=1 Tax=Picea sitchensis RepID=B8LRQ8_PICSI	-	-	94%
FL-Next	sp=Malic enzyme; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	93%
Blast2go	protein	-	-	89%

Source	ECs	Term	Type	e value	Identity
Blast2go		EC:1.1.1.4-	-	0.0	89%

Source	GOs	Term	Type	Identity
Blast2go	malate metabolic process	GO:0006108	Biological Process	89%
Blast2go	oxidation-reduction process	GO:0055114	Biological Process	89%
Blast2go	NAD binding	GO:0051287	Molecular Function	89%
Blast2go	metal ion binding	GO:0046872	Molecular Function	89%
Blast2go	malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity	GO:0004473	Molecular Function	89%
Blast2go	cytoplasm	GO:0005737	Cellular Component	89%

Source	InterPros	Term	Type	Identity
Sma3	Malic oxidoreductase	IPR001891	-	-
Sma3	Aminotransferase, class-II, pyridoxal-phosphate binding site	IPR001917	-	-
Sma3	GNS1/SUR4 membrane protein	IPR002076	-	-
Sma3	Malic enzyme, N-terminal	IPR012301	-	-
Sma3	Malic enzyme, NAD-binding	IPR012302	-	-
Sma3	Malic enzyme, conserved site	IPR015884	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LRQ8

Fln msg: Overlapping hits, possible frame ERROR between 825 and 810, Distance to subject end: 331 aas, your sequence is shorter than subject: 250 - 581

Fln protein: MNGDCKPNAAATVAGGVHDVYGEDRASEEQVITPWSRTVASGYVLLRDPRYNKGLAFTDDERDYHYVRGLLPPAHMSQELQVKRLMHLIRQYNTPLQKYMALMDLEERNERLFYKLMIDHVEELLPIVYTPTVGEACQKYGTIFRRPQGLYISLKEKGKVHDVLRNWPEKNIKVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSECLPxxxxxxxNNEMLLNDEFYIGMKQKRATGQEYDD

Protein Length: 251

Fln nts: CGTCGTGACCTTGGCTGTCACTCATGCAGTCTGTCTATCTATAAATACAATACCAAGGCCTTTGCTGTAATTTGAATCAATCACACCAGCCTGGTCACTCACCATATCTTCAATCTGTGTGGCAGAGTTGCAGGATTTCGCACTTCAGGCGAAACC_-_ATGAATGGAGATTGCAAGCCCAATGCTGCTGCCACTGTTGCAGGAGGCGTCCATGATGTCTATGGCGAAGATAGGGCTTCAGAAGAGCAGGTTATCACTCCTTGGAGCCGAACAGTTGCCAGTGGTTATGTATTGCTGAGAGATCCTCGCTACAACAAAGGTTTGGCATTCACAGATGACGAAAGGGATTATCACTATGTACGAGGTCTTCTACCGCCTGCTCACATGTCACAAGAACTTCAAGTGAAGCGTTTAATGCATCTTATTCGTCAATACAATACACCCTTGCAGAAGTACATGGCTTTGATGGATCTTGAGGAGAGGAACGAAAGGTTATTTTACAAGCTCATGATTGATCATGTCGAAGAGTTGCTTCCTATAGTCTATACACCCACAGTGGGAGAAGCTTGCCAAAAATATGGAACTATTTTTAGGCGTCCACAGGGCCTTTACATAAGCTTAAAAGAAAAGGGCAAGGTTCATGATGTTTTGAGGAACTGGCCTGAAAAGAACATTAAGGTCATTGTTGTTACTGATGGTGAACGCATTTTGGGACTGGGGGACCTTGGATGCCAGGGTATGGGGATCCCTGTTGGAAAACTAGCACTCTACACGGCACTTGGTGGAATCCGCCCTTCTGAGTGCCTTCCAxxxxxxxxxxxxxxxxxxxxxAACAATGAGATGCTGCTAAATGATGAGTTCTACATAGGAATGAAGCAGAAGCGTGCTACAGGCCAGGAATATGATGATC

Fln Alignment:

Contig10091___MNGDCKPNAAATVAGGVHDVYGEDRASEEQVITPWSRTVASGYVLLRDPRYNKGLAFTDDERDYHYVRGLLPPAHMSQELQVKRLMHLIRQYNTPLQKYMALMDLEERNERLFYKLMIDHVEELLPIVYTPTVGEACQKYGTIFRRPQGLYISLKEKGKVHDVLRNWPEKNIKVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSECLPIxxxxxTNNEMLLNDEFYIGMKQKRATGQEYDD
B8LRQ8________________MNGDSKSTGAATLAGGVHDVYGEDRASEEQIVTPWSRTVASGNVLLRDPRYNKGLAFTDDERDYHYVRGLLPPAHMSQELQEKRLMHIIRQYNTPLQKYMALMDLEERNERLFYKLMIDHVEELLPIVYTPTVGEACQKYGTIFRRPQGLYISLKEKGKVYDVLRNWPEKNIKVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSECLPIxxxxxTNNEKLLNDEFYIGLRQKRATGQEYDD

SNPs (tot: 28)

Position	A %	C %	G %	T %	Probability
51		33		66	0.997452
52	66		33		0.997452
53		66		33	0.997452
55	66		33		0.997452
56	66			33	0.997452
58	33		66		0.997452
63			33	66	0.997452
69	66	33			0.997452
71		33		66	0.997452
73		33		66	0.997452
74	33		33		0.997753
75	33		66		0.997452
122			66	33	0.997452
263	66	33			0.997452
265		66		33	0.997452
268	66		33		0.997452
272		33	66		0.997452
276		66	33		0.997452
284		33		66	0.997452
287		33	66		0.997452
288	33			66	0.997452
362		12	87		0.992554
366			12	87	0.992554
369			12	87	0.992554
374		87	12		0.992554
379	12			87	0.992554
381	87			12	0.992554
512		16	83		0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain