SustainPineDB

Assembly Name: uagpf v2.0

UniGene Name: uagpf_v2_unigene7991

Length: 932 nt

UniGene Fasta

Ace file of the UniGene uagpf_v2_unigene7991

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene38	12/13

Source	Descriptions	Term	Type	Identity
AutoFact	AGO1-2 (Fragment) n=1 Tax=Nicotiana benthamiana RepID=Q2LFC3_NICBE	-	-	39%
FL-Next	tr=Argonaute/Zwille-like protein; Picea glauca (White spruce) (Pinus glauca).	-	-	30%
Blast2go	protein argonaute	-	-	57%

Source	GOs	Term	Type	Identity
Blast2go	response to far red light	GO:0010218	Biological Process	57%
Blast2go	RNA interference	GO:0016246	Biological Process	57%
Blast2go	embryo development ending in seed dormancy	GO:0009793	Biological Process	57%
Blast2go	auxin metabolic process	GO:0009850	Biological Process	57%
Blast2go	adventitious root development	GO:0048830	Biological Process	57%
Blast2go	somatic stem cell maintenance	GO:0035019	Biological Process	57%
Blast2go	leaf morphogenesis	GO:0009965	Biological Process	57%
Blast2go	miRNA metabolic process	GO:0010586	Biological Process	57%
Blast2go	gene silencing by miRNA	GO:0035195	Biological Process	57%
Blast2go	response to auxin stimulus	GO:0009733	Biological Process	57%
Blast2go	virus induced gene silencing	GO:0009616	Biological Process	57%
Blast2go	protein binding	GO:0005515	Molecular Function	57%
Blast2go	endoribonuclease activity	GO:0004521	Molecular Function	57%
Blast2go	siRNA binding	GO:0035197	Molecular Function	57%
Blast2go	miRNA binding	GO:0035198	Molecular Function	57%
Blast2go	cytosol	GO:0005829	Cellular Component	57%
Blast2go	nucleus	GO:0005634	Cellular Component	57%

Source	InterPros	Term	Type	Identity
Sma3	Argonaute/Dicer protein, PAZ	IPR003100	-	-
Sma3	Stem cell self-renewal protein Piwi	IPR003165	-	-
Sma3	Domain of unknown function DUF1785	IPR014811	-	-

Full-Lengther Next Prediction

Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: Q4PLA9

Fln msg: Distance to subject end: 665 aas, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 219 - 959

Fln protein: MAMPSSTTGGGAQMVPATPSFAGKFNILRRPGFGNSGQPISLLANHYRVRFQNKELFHYDVTIEPRPPKGFQYKIIYEAFKKYPEAVNNAVPAYDGQKSLYTAKPLSDKQVELDVVLAENEGGGVPSTRRVRTYHVLFRQAATIHLKQLAQYLAGEPVSLPQEALQVMDVVLRQAALRVFVEKGRSFFGKHFGVEKLQGGLWPIGGFINLFGLRKIIFS

Protein Length: 220

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTTTGCGTTGTCTTGGTAAGTTCTGTGACGGCTGTAAGCACTTTTAGCAACACTGTGTCAGTCTGGTCTCCGGTTCAAAACCCGGCAACTAGCAGAACAGTGGCTGAAGTTGTTGTTAGTGAGAGTCGTGTTATATTCGTAAATTCTGTCTCTGTGTCACGGCTTCTGGTACCGTGCGTCACTCTGTTCGCTGTTCGAACATCTTGTACCTCAAGCTTTGAAGAAAGAAAGTAAAAAAGAAAACGAGCA_-_ATGGCTATGCCCAGTTCAACGACTGGCGGTGGAGCACAAATGGTTCCGGCAACCCCTTCTTTTGCCGGAAAGTTTAACATTTTGAGGCGACCTGGCTTTGGCAATTCTGGACAGCCTATTTCCCTCTTGGCAAATCATTACAGGGTTCGGTTTCAGAACAAGGAGTTGTTCCATTACGATGTCACCATTGAGCCGAGGCCTCCTAAGGGTTTCCAATATAAGATTATATATGAAGCATTCAAGAAGTACCCTGAGGCCGTGAACAATGCTGTCCCTGCTTATGATGGGCAGAAGAGCCTCTATACAGCCAAGCCTCTCAGCGACAAGCAAGTGGAACTGGACGTTGTCTTGGCGGAAAATGAAGGCGGAGGAGTTCCTTCCACAAGGCGGGTTAGGACTTACCACGTGCTATTCAGGCAGGCCGCAACGATTCACTTGAAGCAACTGGCACAGTACTTAGCAGGGGAGCCTGTGTCGCTTCCTCAAGAAGCATTGCAGGTTATGGATGTGGTGCTTCGTCAAGCAGCTCTGAGAGTTTTCGTTGAAAAAGGGAGATCGTTCTTTGGGAAACACTTTGGCGTTGAGAAGCTTCAGGGGGGTTTGTGGCCTATCGGGGGTTTTATCAATCTATTCGGCCTGCGCAAGATAATCTTCTCAC

Fln Alignment:

Contig7991___ILRRPGFGNSGQPISLLANHYRVRFQN-KELFHYDV--TIEPRPPKGFQYKIIYEAF--KKYPEAVNNAVP---------AYDGQKSLYTAKPLSDKQVELDVVLAENEGGGVP---------------STRRVRTYHVLFRQAATIHLKQLAQYLAGEPVSLPQEALQVMDVVLRQAALRV-FVEKGRSFFGKHFG-VEKLQGGLWPIGGF
Q4PLA9_______________LTKRPGHGRAGRPVKLLCNHFRVSFSNLQDSFHYDVKITASERVERGGKAQDVVSKILCRKVMDKVKEVYGATGLDGKDFAYDGENSLFTVGPLRNNNIECIIVIDDERSSRRPIKDESPSQIEQSKKRREDRGEKFKVEITFAAKISMKALQAVIQGEVSDKAQDALRVLDIVLRQHASRKGYLLVRQSFFHWSFAPLVELGGGVTGCRGY

SNPs (tot: 56)

Position	A %	C %	G %	T %	Probability
25	75			25	0.996473
116	71	28			1.0
120	71		28		1.0
121			71	28	1.0
122	71		28		1.0
125	71	28			1.0
126			71	28	1.0
129	28		71		1.0
134	71	28			1.0
136		28	71		1.0
139	28			71	1.0
155		42		57	1.0
414	80		20		0.995493
416		20		80	0.995493
417	80		20		0.995493
418		80		20	0.995493
419	80		20		0.995493
420	20		80		0.995493
421		20	80		0.995493
422		20	80		0.995493
425		80	20		0.995493
427		25	75		0.996473
429			25	75	0.996473
433		25	75		0.996473
434	75			25	0.996473
435	75	25			0.996473
436	25	75			0.996473
456	80			20	0.995493
457		20		80	0.995493
460		80	20		0.995493
464	66		16		0.988813
466		16		83	0.994512
468	66			16	0.988813
471		83		16	0.994512
472			83	16	0.994512
473	66			16	0.988813
474			83	16	0.994512
475		16	83		0.994512
476	16	83			0.994512
477		83	16		0.994512
480	16	83			0.994512
482	83	16			0.994512
532		25	75		1.0
534		75		25	1.0
540	75	25			1.0
542	75			25	1.0
543	75			25	1.0
544			25	75	1.0
551	25	75			1.0
554		25	75		1.0
555	25	75			1.0
729	85			14	0.993533
730			85	14	0.993533
731		14		85	0.993533
732	85		14		0.993533
760			40	60	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain