SustainPineDB

Assembly Name: uagpf v2.0

UniGene Name: uagpf_v2_unigene7631

Length: 1050 nt

UniGene Fasta

Ace file of the UniGene uagpf_v2_unigene7631

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene1648	23/23

Source	Descriptions	Term	Type	Identity
AutoFact	Isoamylase isoform 3 n=1 Tax=Solanum tuberosum RepID=Q84YG5_SOLTU	-	-	74%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	31%
Blast2go	isoamylase chloroplastic-like	-	-	85%

Source	ECs	Term	Type	e value	Identity
Blast2go	Alpha-amylase.	EC:3.2.1.1	-	0.0	85%

Source	KEGGs	Term	Type	Identity
Blast2go	Starch and sucrose metabolism	00500		85%
Blast2go	Metabolic pathways	01100		85%
Blast2go	Isoamylase.	EC:3.2.1.68	-	85%

Source	GOs	Term	Type	Identity
Blast2go	glycogen catabolic process	GO:0005980	Biological Process	85%
Blast2go	starch catabolic process	GO:0005983	Biological Process	85%
Blast2go	cation binding	GO:0043169	Molecular Function	85%
Blast2go	alpha-amylase activity	GO:0004556	Molecular Function	85%
Blast2go	isoamylase activity	GO:0019156	Molecular Function	85%
Blast2go	chloroplast stroma	GO:0009570	Cellular Component	85%
Blast2go	chloroplast starch grain	GO:0009569	Cellular Component	85%

Source	InterPros	Term	Type	Identity
Sma3	Aminoacyl-tRNA synthetase, class I, conserved site	IPR001412	-	-
Sma3	Glycoside hydrolase, family 13, N-terminal	IPR004193	-	-
Sma3	Glycosyl hydrolase, family 13, catalytic domain	IPR006047	-	-
Sma3	Alpha-amylase, C-terminal all beta	IPR006048	-	-
Sma3	Glycosyl hydrolase, family 13, all-beta	IPR013780	-	-
Sma3	Glycoside hydrolase, subgroup, catalytic domain	IPR013781	-	-
Sma3	Immunoglobulin-like fold	IPR013783	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5ABA9

Fln msg: No stop codon before M and low homology subject, your sequence is longer than subject: 213 - 175

Fln protein: MNGESGRDGCNDNYSWNCGFEGETRDPAINALRSRQMKNFHLALMVSQGTPMILMGDEYAHTRYGNNNSYGHDTAMNHFQWNQLDARKESHFRFFAETIRFHRQHPLLGRETFLTKADITWHEKNWDNFDSRFLAFSLHEGKYGGGDLYIAFNAHDYFVKSVVPPPPSGKCWYRVVDTNLPSPDDFVVGGVPGVKKVYEMAPYSGVVLHAKP*

Protein Length: 214

Fln nts: CGTCGTGACCTTGGCTGTCACTCATACTTTTGCTACACGTATAGCAGGGTCTTCTGATTTGTATCAGATAAATGCACGCAAGCCATATCACAGCATCAATTTTGTTATTGCACATGATGGATTTACTTTATATGATCTTGTTGCTTATAACTCGAAGCACAATGAA_-_ATGAATGGAGAAAGTGGAAGGGATGGATGCAATGATAATTACAGCTGGAATTGTGGTTTTGAAGGTGAGACAAGGGATCCTGCCATCAATGCTCTCCGTTCACGTCAGATGAAAAATTTCCATTTAGCATTAATGGTTTCACAGGGAACACCTATGATTTTAATGGGAGATGAGTATGCTCACACTCGATATGGAAATAATAACAGTTATGGTCATGATACTGCCATGAACCATTTCCAGTGGAATCAGCTAGATGCAAGAAAGGAGAGTCACTTCAGGTTTTTTGCTGAGACTATTAGATTTCACCGACAACATCCTCTTTTGGGGCGTGAGACATTTCTAACCAAGGCTGATATCACTTGGCACGAGAAGAATTGGGATAATTTTGATAGCAGGTTTCTTGCATTTTCATTGCATGAAGGAAAATACGGAGGTGGAGATCTTTACATAGCTTTCAATGCACATGATTATTTTGTAAAATCTGTCGTGCCTCCACCACCCTCAGGAAAATGCTGGTATCGAGTGGTGGATACCAATCTTCCATCTCCAGATGACTTCGTTGTTGGAGGAGTTCCAGGCGTCAAAAAAGTTTATGAGATGGCTCCATATTCTGGAGTTGTCCTTCATGCAAAACCCTAAACTGAAATTCTAAAATATTACCAGTGCAAATCTTTGCAATCACTGCATTACAATTATTTTGGCCATATTCCCATGTGCTTAAAGCTTATTTAAGGTTCTACACAGACATATTTAGTCTGGCAAATGCCAATGGTATTGATCATGTCGAAGTGTCTATCTGAGAGCCTATTGCATGAACGTATGGACATGTGCCCAGATTTTCCCTTATTATTATATGAATTTCTAAACTTATTCAATAGTTTGAA

Fln Alignment:

Contig7631___MGDEYAHTRYGNNNSYGHDTAMNHFQWNQLDARKESHFRFFAETIRFHR--------QHPLLGRETFLTKADITWH-----EKNWDNFDSRFLAFSL------HEGKYGGGDLYIAFNAHDYFVKXXXXXXXXGKCWYRVVDTNLPSPDDF-----XXXXXXXXXXXYEMAPYSGVVLHAK
D5ABA9_______________MGDEYGQTKGGSMLLNDRKP----FNWDALKTE------FAVETVRFIRFLSSFRTYRRDLLQRKTFAKIENLNWHGPIPDQPQWEDPNSSFLAISLKPERNENESRSTVGDLYIAFNSQKASVSATLPQLPDGMLWHRLVDTSLPFPKTFSMEGMATDMLGISFSTYQIQPHSCVLFEAR

SNPs (tot: 44)

Position	A %	C %	G %	T %	Probability
291	88	11			0.923564
292		11		88	0.923564
295	88		11		0.923564
298	88			11	0.923564
299			11	88	0.923564
301	88	11			0.923564
303		11		88	0.923564
304			88	11	0.923564
305		11	88		0.923564
306	11			88	0.923564
435	83	16			0.994512
437	83	16			0.994512
440		83	16		0.994512
443		16		83	0.994512
445		83		16	0.994512
446	83		16		0.994512
448		16	83		0.994512
450			16	83	0.994512
452		16		83	0.994512
453	16			83	0.994512
454		16		83	0.994512
455			83	16	0.994512
457	16			83	0.994512
464	85	14			0.993533
465			14	85	0.993533
470		14		85	0.993533
473		85	14		0.993533
474	85		14		0.993533
476		85		14	0.993533
478	85			14	0.993533
486	16	83			0.994512
758		33	66		0.997452
759			33	66	0.997452
761		33		66	0.997452
766		33	66		0.997452
767	66			33	0.997452
774		66	33		0.997452
775	66			33	0.997452
776		33		66	0.997452
778		33		66	0.997452
782	33		66		0.997452
1017		25		75	0.996473
1018	75		25		0.996473
1019		25		75	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain