SustainPineDB

Assembly Name: uagpf v2.0

UniGene Name: uagpf_v2_unigene5652

Length: 1210 nt

UniGene Fasta

Ace file of the UniGene uagpf_v2_unigene5652

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene7837	48/52

Source	Descriptions	Term	Type	Identity
AutoFact	DNA-directed RNA polymerase II subunit E [Arabidopsis thaliana] gb\|AAK48978.1\|AF370551_1 RNA polymerase I, II and III 24.3 kDa subunit [Arabidopsis thaliana] gb\|AAL06554.1\|AF412101_1 AT3g22320/MCB17_5 [Arabidopsis thaliana] gb\|AAC28253.1\| RNA polymerase I	-	-	70%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	97%
Blast2go	dna-directed rna polymerases and iii subunit rpabc1	-	-	86%

Source	ECs	Term	Type	e value	Identity
Blast2go	DNA-directed RNA polymerase.	EC:2.7.7.6	-	0.0	86%

Source	KEGGs	Term	Identity
Blast2go	Purine metabolism	00230	86%
Blast2go	Pyrimidine metabolism	00240	86%
Blast2go	Metabolic pathways	01100	86%

Source	GOs	Term	Type	Identity
Blast2go	transcription, DNA-dependent	GO:0006351	Biological Process	86%
Blast2go	DNA-directed RNA polymerase activity	GO:0003899	Molecular Function	86%
Blast2go	DNA binding	GO:0003677	Molecular Function	86%
Blast2go	protein binding	GO:0005515	Molecular Function	86%
Blast2go	DNA-directed RNA polymerase II, core complex	GO:0005665	Cellular Component	86%
Blast2go	cytoplasmic membrane-bounded vesicle	GO:0016023	Cellular Component	86%
Blast2go	DNA-directed RNA polymerase IV complex	GO:0000418	Cellular Component	86%

Source	InterPros	Term	Type	Identity
Sma3	DNA-directed RNA polymerase, RPB5 subunit	IPR014381	-	-
Sma3	RNA polymerase, Rpb5, N-terminal	IPR005571	-	-
Sma3	RNA polymerase, subunit H/Rpb5 C-terminal	IPR000783	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LR68

Fln msg: Separated hits, possible frame ERROR between 470 and 502,

Fln protein: MALDEQTNKLFRIRRTVLQMLNDRDYLVGDFEINTTKEQFLDKFGEDPKKEDLVINKAKRNNPSEQLFVFFPEEAKVGVKTIKTYVERMKSEGVFRAILVVQxxxxxxxxxxxxQEFSGKFHLEVFQEAELLVNIKEHVLVPEHQILTPEEKKTLLERYTLKETQLPRMQESDPVARYYGLKRGQVVKIIRPSETAGRYVTYRYVV*

Protein Length: 208

Fln nts: CGTCGTGACCTTGGCTGTCACTCAAGATTAATTTCCTTGTTCTGCGAGTCTTTGAGTGCAAAAGGTTCCATTTGAGAATTCCATAAAAGGGTTCTCCATAAAACGGGTTTGGGTTAACAACTCCACTTTGAAGGCGACGGAATTAAGCTGAGCAGCTCGCA_-_ATGGCGCTGGATGAGCAGACAAACAAGCTCTTTAGAATCCGACGTACAGTTCTTCAGATGCTCAATGACAGAGATTATCTCGTGGGTGATTTTGAGATCAACACGACCAAAGAGCAGTTCCTGGATAAGTTCGGAGAAGACCCAAAGAAGGAAGATCTTGTTATCAATAAAGCCAAGCGCAACAATCCTTCAGAACAGTTATTCGTCTTCTTTCCGGAAGAGGCGAAAGTGGGAGTGAAGACGATCAAAACATATGTGGAACGTATGAAATCTGAGGGTGTGTTTCGAGCTATATTGGTGGTGCAGCAxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxCCAAGAGTTCTCGGGAAAATTTCATTTGGAAGTATTCCAGGAGGCAGAGTTGCTTGTTAACATAAAGGAACATGTTCTTGTGCCAGAGCACCAAATACTCACTCCTGAAGAGAAGAAGACATTATTGGAGAGATATACTCTGAAAGAAACACAGCTTCCACGAATGCAAGAGTCTGATCCTGTTGCACGGTATTATGGGCTCAAGCGTGGTCAAGTTGTTAAGATTATCCGACCTAGTGAGACGGCTGGAAGATATGTGACTTACCGTTATGTTGTTTAA___ATCTAGTTTGAACATGATCAAAGTTTTAACATTTTGAATGACTGTTACTTTGGCTAAATAAACTCTTTTAGCCACTTTGGAGCCTTGTAACAGTGCTAATGATGAAAACTGTTGAATGGATGTGTTTTTGTATTATAGTTCCATAAGCCAGCCAGTCTAGGCAGGATTGAGGCTTAAACTATAAACTTCATTAATTTATTTCTTATTGCAAGTGCTGCAATTATGGAATCTGAGTAAAATTGTGAAAGACAGGTGAACTGATGGCCATTATGCCACATCTCTCTGGCCTGTTTAATGCTTCTCATCTTCCATCAGTCATATGTGATTTTATTCCATCATTTTTAGTTTGGTGAAATTTGAAAAAGTGCTGTATCATTCTGTAGATGCGATTTGAAGTATATGATTTCCATCCATACAGCGTACCTATACA

Fln Alignment:

Contig5652___MALDEQTNKLFRIRRTVLQMLNDRDYLVGDFEINTTKEQFLDKFGEDPKKEDLVINKAKRNNPSEQLFVFFPEEAKVGVKTIKTYVERMKSEGVFRAILVVQQxxxxxxxxxxxQEFSGKFHLEVFQEAELLVNIKEHVLVPEHQILTPEEKKTLLERYTLKETQLPRMQESDPVARYYGLKRGQVVKIIRPSETAG
B8LR68_______________MALDEQTNKLFRIRRTVLQMLNDRDYLVGDFEINTTKEQFLDKFGEDPKKEDLVINKAKRNSPSEQIFVFFPEEAKVGVKSIKTYVERMKSEGVFRAILVVQQxxxxxxxxxxxQEFSGKFHLEVFQEAELLVNIKEHVLVPEQQILTPEEKKTLLERYTLKETQLPRMQESDPVARYYGLKRGQVVKIIRPSETAG

SNPs (tot: 54)

Position	A %	C %	G %	T %	Probability
115		16		83	0.994512
117	83		16		0.994512
118	83			16	0.994512
133	83			16	0.994512
210	50		50		1.0
366		88		11	0.923564
370		11	22	66	0.999999
372		77	22		0.999999
373	22			77	0.999999
374		22		77	0.999999
375		22		77	0.999999
376		77		22	0.999999
377		77		22	0.999999
380	77	22			0.999999
381	77			22	0.999999
383	77			22	0.999999
384		22	77		0.999999
385	22		77		0.999999
388	77			22	0.999999
389	77	22			0.999999
432	87	12			0.992554
433	87		12		0.992554
439		14	85		0.993533
440		14	85		0.993533
441			85	14	0.993533
444			14	85	0.993533
445		14	85		0.993533
447			14	85	0.993533
450	14		85		0.993533
451	85	14			0.993533
452			85	14	0.993533
454	14			85	0.993533
521	83			16	0.994512
522	83	16			0.994512
523	83		16		0.994512
524		16		83	0.994512
574	78	7			0.999974
763		11		88	0.999952
765	88			11	0.999952
768			12	87	0.999964
776			12	87	0.999964
778		13		86	0.999973
782	15			84	0.999986
783		15		84	0.999986
784	84			15	0.999986
785	84	15			0.999986
907	11		88		0.923564
1057			83	16	0.999991
1097	10			90	0.90298
1098		90		10	0.90298
1100	10		90		0.90298
1101	10			90	0.90298
1107			88	11	0.923564
1114		16		83	0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain