SustainPineDB

Assembly Name: uagpf v2.0

UniGene Name: uagpf_v2_unigene5103

Length: 959 nt

UniGene Fasta

Ace file of the UniGene uagpf_v2_unigene5103

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene21787	16/18

Source	Descriptions	Term	Type	Identity
AutoFact	putative pectinesterase 68 [Arabidopsis thaliana] sp\|Q8LPF3.1\|PME68_ARATH RecName: Full=Probable pectinesterase 68; Short=PE 68; AltName: Full=Pectin methylesterase 68; Short=AtPME68; Flags: Precursor gb\|AAM26686.1\| AT5g47500/MNJ7_9 [Arabidopsis thaliana]	-	-	43%
FL-Next	sp=Pectinesterase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	41%
Blast2go	protein	-	-	63%

Source	InterPros	Term	Type	Identity
Sma3	Pectinesterase, catalytic	IPR000070	-	-
Sma3	Ribosomal protein S17	IPR000266	-	-
Sma3	Alcohol dehydrogenase, zinc-type, conserved site	IPR002328	-	-
Sma3	TonB box, conserved site	IPR010916	-	-
Sma3	Pectin lyase fold	IPR012334	-	-
Sma3	Nucleic acid-binding, OB-fold	IPR012340	-	-
Sma3	Pectinesterase, active site	IPR018040	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LRK3

Fln msg: your sequence is shorter than subject: 281 - 344

Fln protein: YRPWLSLIPANNNNPVTIKVNPGTYKEKIEIPVGMSHITLTGSRRDLGCHSWNDDAQSSGGTFLSATVNVLASDFTARDITIQNSYGQGDLSGNNQAVALKISGDRCAFYNCRILGFQDTLLDHIGRHYFKNCYIEGAVDFIFGNGKSIYQSCELHAIPVYYGTFTAQNRGSPSEDTGFVFLNCRLTGKGPNYLGKPWGDYSTVVFANTYMEDIIYPVGWGDWDTDFYKQKSVTYGEFQNSGPGAVRKQRVAWSQKLTTRSQFSKYLTLDYINGSTWIEQ*

Protein Length: 282

Fln nts: TGTATCGACCTTGGCTGTCACTCATTCCTGCTAATAACAACAACCCGGTAACCATTAAAGTCAATCCCGGCACTTACAAGGAGAAGATAGAAATTCCTGTAGGTATGTCACACATAACTCTTACTGGCTCACGTCGTGACCTTGGCTGTCACTCATGGAATGATGATGCGCAATCGAGCGGTGGGACATTTTTGTCGGCAACGGTCAACGTGCTTGCTTCGGATTTCACAGCCAGAGATATCACAATTCAGAATTCATATGGTCAAGGTGACTTGAGTGGAAATAATCAAGCTGTGGCGTTGAAGATTTCGGGAGATCGTTGCGCGTTTTACAATTGCAGAATTCTAGGGTTTCAAGACACCTTGTTAGACCACATTGGGAGGCATTACTTTAAAAACTGTTACATTGAAGGTGCCGTCGACTTTATATTTGGCAATGGGAAATCTATCTATCAGAGCTGCGAACTACATGCGATCCCTGTATATTATGGCACATTTACGGCTCAGAATAGAGGCTCTCCGTCCGAAGACACGGGCTTCGTCTTTTTAAATTGCAGGTTAACGGGGAAGGGACCAAATTATCTGGGCAAACCCTGGGGAGATTACTCAACAGTAGTGTTTGCTAACACTTACATGGAAGACATTATATACCCAGTAGGATGGGGAGACTGGGATACGGACTTTTACAAACAAAAATCTGTGACTTATGGCGAGTTCCAAAATAGCGGGCCTGGAGCAGTGCGTAAACAACGAGTTGCCTGGTCCCAAAAACTCACGACTCGTTCGCAGTTCTCAAAGTATTTGACCCTCGACTACATTAATGGGAGCACATGGATTGAACAATAG___GGCATCCACCGTAACAGAAGAAGAAAGTGGTATCCTGAAGAAAGTGATATCCTGAAGGCATTCACACAAAATGATTTCCATAAGCAAGAAAAATGCGAGTGGCCATTCTTCGCT

Fln Alignment:

Contig5103___IPANNNNPVTIKVNPGTYKEKIEIPVGMSHITLTGSRRDLGCHSWNDDAQSSGG------TFLSATVNVLASDFTARDITIQNSYGQGDLSGNN--QAVALKISGDRCAFYNCRILGFQDTLLDHIGRHYFKNCYIEGAVDFIFGNGKSIYQSCELHAIPVYYGTFTAQNRGSPSEDTGFVFLNCRLTGKGPNYLGKPWGDYSTVVFANTYMEDIIYPVGWGDWDTDFYKQKSVTYGEFQNSGPGAVRKQRVAWSQKLTTRSQFSKYLTLDYINGSTWIE
B8LRK3_______________IPNGNRERVIIEIRPGFYQEKVLVPQAKPYIIFQGAGMGRTVIEWHNKASDVGADGQELHTYNTASVTVLANHFTAKNISFKNS-APAPLPGMEGWQAASFRISGDKAYFLGCGFYGAQDTLCDDAGRHFFKECFIQGSIDFIFGNARSIYYKCELHSIARVFGAIAAQARTMPNEDTGFSFLHCKVTGTGPLYLGRAMGQYSRIVYAYSYFDDII--AGWDDW-AQTSKDGTVFFGLYNCYGPGARAAQQISWVHELTP-AQAQPFLVKTFINGRHWLE

SNPs (tot: 51)

Position	A %	C %	G %	T %	Probability
132		66	33		0.997452
134		33		66	0.997452
135	33	66			0.997452
141		66		33	0.997452
142	33			66	0.997452
143		33		66	0.997452
144		33	66		0.997452
145	33		66		0.997452
147		33		66	0.997452
154	33			66	0.997452
156	66			33	0.997452
168		25		75	0.996473
173		75		25	0.996473
174	75		25		0.996473
176		25		75	0.996473
179			75	25	0.996473
180	75		25		0.996473
183			75	25	0.996473
186		25	75		0.996473
187	25		75		0.996473
188		25	75		0.996473
189	75			25	0.996473
360	80			20	0.995493
361	80	20			0.995493
363	80			20	0.995493
364		80	20		0.995493
371			25	75	0.996473
372		25		75	0.996473
373	75			25	0.996473
375	75			25	0.996473
379	75			25	0.996473
458		33		66	0.997452
459	66		33		0.997452
462	66			33	0.997452
464	85	14			0.993533
465		14	85		0.993533
466		85		14	0.993533
467		14		85	0.993533
468			85	14	0.993533
469		71	14	14	0.984337
471	85	14			0.993533
472	71	14		14	0.984337
473		85	14		0.993533
475	71		14		0.984337
476		85	14		0.993533
477	85	14			0.993533
478		14		85	0.993533
479			85	14	0.993533
481	28		71		1.0
482	85	14			0.993533
863		83	16		0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain