SustainPineDB

Assembly Name: uagpf v2.0

UniGene Name: uagpf_v2_unigene3163

Length: 804 nt

UniGene Fasta

Ace file of the UniGene uagpf_v2_unigene3163

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene8351	7/9

Source	Descriptions	Term	Type	Identity
AutoFact	mitogen-activated protein kinase kinase [Suaeda salsa]	-	-	60%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	39%
Blast2go	mitogen-activated protein kinase kinase 6-like	-	-	73%

Source	GOs	Term	Type	Identity
Blast2go	induced systemic resistance, jasmonic acid mediated signaling pathway	GO:0009864	Biological Process	73%
Blast2go	MAPK cascade	GO:0000165	Biological Process	73%
Blast2go	induced systemic resistance, ethylene mediated signaling pathway	GO:0009866	Biological Process	73%
Blast2go	phosphorylation	GO:0016310	Biological Process	73%
Blast2go	defense response, incompatible interaction	GO:0009814	Biological Process	73%
Blast2go	MAP kinase kinase activity	GO:0004708	Molecular Function	73%
Blast2go	nucleotide binding	GO:0000166	Molecular Function	73%

Source	InterPros	Term	Type	Identity
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Serine-threonine/tyrosine-protein kinase catalytic domain	IPR001245	-	-
Sma3	Nuclear transport factor 2	IPR002075	-	-
Sma3	Serine/threonine- / dual-specificity protein kinase, catalytic domain	IPR002290	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-
Sma3	Nuclear transport factor 2, Eukaryote	IPR018222	-	-

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: D5A9P4

Fln msg: Separated hits, possible frame ERROR between 400 and 403, Unexpected stop codon in the beginning of your sequence, Distance to subject end: 31 aas, your sequence is shorter than subject: 268 - 349

Fln protein: VVTLAVTQ*YE*ETRQ*PWHVICIFGVIGRGASSVVQKAIHIPTHRILALKKINTFEKEKRHQLLNEIRTLCEAPHVKGLVEFHGAFYTPASGQISIALEYMDGGSLRDLVQSKKCIPEPILSVITHEILHGxxFLHHVRHLVHRDIKPANLLINLNGEPKITDFGISVGLENTVAMCGTFVGTVTYMSPERIGNEYYSFPADIWSLGLSIFECGTGEFPYNASKGPVNLMLQVIYDPSPSPSRDCFSEEFCSFVDVCLQRIQLQGLR

Protein Length: 269

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATGATACGAATGAGAAACACGTCAGTGACCTTGGCATGTCATCTGCATATTTGGTGTCATTGGACGTGGTGCAAGCAGTGTTGTCCAAAAGGCTATTCATATACCAACACATCGGATTTTAGCACTGAAGAAAATAAATACTTTTGAGAAGGAAAAACGTCACCAGCTATTAAATGAGATTCGAACACTATGTGAGGCACCACATGTGAAAGGCTTAGTGGAGTTTCATGGGGCTTTTTACACTCCTGCATCTGGACAAATCAGCATTGCTTTAGAATACATGGATGGAGGCTCACTTAGAGATCTTGTGCAATCAAAGAAGTGTATTCCTGAGCCAATTCTTTCTGTTATTACACATGAAATTTTACATGGATTxxxATTTTTACATCACGTGAGGCATCTGGTGCATAGAGACATAAAACCTGCTAATCTGCTTATAAACCTTAATGGAGAGCCAAAAATTACAGATTTTGGAATTAGTGTTGGTTTGGAGAACACTGTTGCGATGTGTGGCACATTTGTTGGGACCGTCACATACATGTCACCAGAGAGAATTGGTAATGAATATTATTCATTCCCAGCAGATATCTGGAGCCTAGGACTTTCCATTTTTGAATGTGGTACAGGAGAGTTCCCATACAATGCAAGCAAGGGCCCTGTGAATCTCATGCTTCAGGTCATATATGATCCATCTCCCTCACCTTCACGAGATTGCTTTTCAGAGGAGTTTTGCTCATTCGTTGATGTCTGTCTACAGAGGATCCAACTGCAAGGCCTTCGC

Fln Alignment:

Contig3163___VIGRGASSVVQKAIHIPTHRILALKKIN-TFEKEKRHQLLNEIRTLCEAPHVKGLVEFHGAFYTPASGQISIALEYMDGGSLRDLVQSKKCIPEPILSVITHEILHGLxFLHHVRHLVHRDIKPANLLINLNGEPKITDFGISVGLENTVAMCGTFVGTVTYMSPERIGNEYYSFPADIWSLGLSIFECGTGEFPY---NASKGPVN---LMLQVIYDPSPSPSRDCFSEEFCSFVDVCLQR
D5A9P4_______________VIGKGSGGVVQLVRHKWTGQFFALKAIQMSIQESVRKQIVQELK-INQASQCPNVVVCYHAFYN--NGVISIVLEYMDCGSLADVIKRVKTFTEPYLAVICKQVLKGLxYLHRDRHIIHRDIKPSNLLVNHKGEVKITDFGVSATLANSMGQRDTFVGTYNYMSPERISGSTYGFSSDIWSLGLVVLECATGRFTYLPPGQEEGWLNFYELLETIVEQPAPCASPDQFSPEFCSFISACVQK

SNPs (tot: 22)

Position	A %	C %	G %	T %	Probability
46		66		33	0.997452
47	33		66		0.997452
54	66			33	0.997452
55		66	33		0.997452
59		33	66		0.997452
65	33			66	0.997452
66		66	33		0.997452
69		66	33		0.997452
116	85	14			0.993533
118			85	14	0.993533
120			14	85	0.993533
121	85			14	0.993533
122			14	85	0.993533
123	14			85	0.993533
125	85	14			0.993533
127	85			14	0.993533
128			14	85	0.993533
129	85		14		0.993533
131		85		14	0.993533
132	85		14		0.993533
133	85			14	0.993533
141	14		85		0.993533

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain