SustainPineDB

Assembly Name: uagpf v2.0

UniGene Name: uagpf_v2_unigene6331

Length: 2348 nt

UniGene Fasta

>uagpf_v2_unigene6331
CGTCGTGACCTTGGCTGTCACTCAGGATCTGATGGCAGTGATTAGGACTGTTGCTTATTGTTTGTAAGTTTCTCAGCAACGAAGAAGCCACATAATACGTCGTGACCTTGGCTGTCACTCAACATTATTGTGTACGGTTTTGCAGTGTACAGTGCGCAATGCAATCTGTGAACCACAATGAACTAATTGTTTTACTCGGTGTTTTGAAGATTCTGGTGGTGGAGGTAATCAGTTCCGCCCAATAAGCTATTAAGTTGGTGTAAGAGACGGTAATTAAGTTTGGTTTTGGCTTTGTTTACATCTAACTGGACACTGTGATTGGATGATTTATTGTGGATTCGAGAGGGTAGGGGCTAGATTTTGCTATAATTAGTCATTCATAGATAGCTGTGGAAAAACGCTTTTATCTGCTCCATGATGGGTGCTTTTCCTTCAGCGAAGATGCTTGTTATAGGTGTAGTCTGATGGGGAAAAGTCGGTTCCCTGCCTATACAGCTGATGAAGGTTCTATGGTGTGACCAAATTGGCCAGATTCTTGTTCTGTGATTTGGGTTCTTTGCAGAACTGAGAAATTTTGGTCCTGTATTGAGGTTATTTAGCCCAGGCTTAATTTATTTTAAGTCTGAGAAGCTGCATTGAGATTTTATAAACTGGCGATGATAAAGAAGAAGCAGAAGGATTTTGATAAGGTTGTATGGGATCAGATGGCTGGAAGGAAAGTCCACCCATCTGCTGGTATGCTTTCCCGCATTGGGGGTTGGGCTAGTAAGGTTTTTAGATCGCTCATTGTGTTGATCTGTATATCTTACTTCATATATCTTATGAATCTTATGCTAAGCAATTACCCATGTTGGCAACCCATCATAAGTTGCAGAACCGAGTGTACTCAGTCGGCTAGTTCTAGTTCTAGTTCTAGTACTAGTACTACTAACAGTGTGGCAGTTTTAGCCGTGGAGAATACTACGAAGGTTGTCAATGCCGACGAAGCTGGTATTCACAGGCAGGCGCTTCAAGCTATTCCTGATTCTACAGGAACTGACTTGAAGCACATAGTGTTTGGGATTGCCGCCTCTGCTAGGTTATGGGACAAGAGAAAAGAGTACATAAAGTTGTGGTGGAGGCCGAAGCAGATGAGGGGGTTTATTTGGTTAGAGAAGCCTGTGAAAACTGGCAAGGGTGACAATTCTTCCCTGCCCCCATGGAGAATATCAGCCAATACCTCCCATTTTAAGTACACCAACAAGCAGGGACACAGATCTGCCATTAGAATTTCCCGCATTGTCTCTGAGACTTTCAGAATGGGTCTTCCTGATGTGCATTGGTTTGTCATGGGGGATGATGATACTGTCTTTATTCCTGATAATCTGGTGACGGTTCTGTCCAAGTATGATCACACGAAACCTTACTACATTGGCAGTTTATCAGAGTCTCATTTGCAGAATATATTCTTTTCTTATAGTATGGCTTATGGGGGAGGCGGATTTGCCATTAGCTATCCTCTGGCAAAGGCGTTGGAGACTATGCAAGATAAGTGTATTCAGAGGTATCCTGGTTTGTATGGAAGCGATGATAGAATGACAGGCCTGCATGTCTGAGTTGGGCGTGCCGCTTACAAAGGAGCCTGGCTTTCATCAGTATGATGTATATGGTAATTTATTCGGATTACTGGCAGCTCATCCTTTAACTCCCTTGGTGTCACTTCACCATTTGGATGTTGTGGAACCTATATTCCCAAACATGACCAGAGTAAAGGCACTGCAGCACTTAAGCAAAGCTGTAAAATTAGATTCAGCTGGAATAATGCAACAGTCTATTTGTTATGATAAAAAGCGTGAATGGTCTATATCAGTCTCATGGGGTTATGCAGTTCAGATCTACAGAACGATATTTTCCCCTCGTGAGTTGGAGATGCCCTCCAGGACGTTCTTGAATTGGTACAGAAGAGCAGACTTTACAGCATATGCCTTCAATACCAGGCCCGTGACAAGACATCCATGCCAGAAACCCTTCATATTTTATTGAAGGATGTTCGCCATGATAATGCGAGTGGACAAATCATAAGCAATTATGCTAGACAGCAAGTAAAGCCTCCTGGATGCAGGTGGAGAATACCTTCCCCAGAGATCATAAATTTGATTCGGGTTGTGAAGAAACCTGATACATTATTATGGCAGTCACCACGGCGAAACTGCTGCAGAGTGGTAGCATCCAAAAAGTCCAGCCTTGATATTGAAGTGGGTGCCTGCCGGGAAGGTGAGATCATTGGTGTATCATAGCACATCTGCTGTACAAGAATGTCTTTATCAGAAGGCCTATGCACGCATTTTGGGAGTAGATCAGCCAGCTTC

Ace file of the UniGene uagpf_v2_unigene6331

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene5630	52/55
SustainPine v2.0	sp_v2.0_unigene21657	1/55

Source	Descriptions	Term	Type	Identity
AutoFact	WS03717.C21_H15 SS-B-N-22 Picea sitchensis cDNA clone WS03717_H15 3', mRNA sequence.	-	-	95%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	51%
Blast2go	uncharacterized protein	-	-	75%

Source	GOs	Term	Type	e value	Identity
Blast2go	transferase activity, transferring glycosyl groups	GO:0016757	Molecular Function	0.0	75%

Source	InterPros	Term	Type	Identity
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Serine-threonine/tyrosine-protein kinase catalytic domain	IPR001245	-	-
Sma3	Homeodomain	IPR001356	-	-
Sma3	Gnk2-homologous domain	IPR002902	-	-
Sma3	Fringe-like	IPR003378	-	-
Sma3	POX	IPR006563	-	-
Sma3	Protein of unknown function DUF604	IPR006740	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3		IPR012287	-	-
Sma3	Homeobox, conserved site	IPR017970	-	-
Sma3	DNA methylase, N-4 cytosine-specific, conserved site	IPR017985	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LNL2

Fln msg: Separated hits, possible frame ERROR between 1577 and 1579, query STOP codon too far from subject stop. Distance to subject end: 90 aas, putative chimeric sequence, your sequence is shorter than subject: 456 - 492

Fln protein: MIKKKQKDFDKVVWDQMAGRKVHPSAGMLSRIGGWASKVFRSLIVLICISYFIYLMNLMLSNYPCWQPIISCRTECTQSASSSSSSSTSTTNSVAVLAVENTTKVVNADEAGIHRQALQAIPDSTGTDLKHIVFGIAASARLWDKRKEYIKLWWRPKQMRGFIWLEKPVKTGKGDNSSLPPWRISANTSHFKYTNKQGHRSAIRISRIVSETFRMGLPDVHWFVMGDDDTVFIPDNLVTVLSKYDHTKPYYIGSLSESHLQNIFFSYSMAYGGGGFAISYPLAKALETMQDKCIQRYPGLYGSDDRxxQACMSELGVPLTKEPGFHQYDVYGNLFGLLAAHPLTPLVSLHHLDVVEPIFPNMTRVKALQHLSKAVKLDSAGIMQQSICYDKKREWSISVSWGYAVQIYRTIFSPRELEMPSRTFLNWYRRADFTAYAFNTRPVTRHPCQKPFIFY*

Protein Length: 457

Fln nts: CGTCGTGACCTTGGCTGTCACTCAGGATCTGATGGCAGTGATTAGGACTGTTGCTTATTGTTTGTAAGTTTCTCAGCAACGAAGAAGCCACATAATACGTCGTGACCTTGGCTGTCACTCAACATTATTGTGTACGGTTTTGCAGTGTACAGTGCGCAATGCAATCTGTGAACCACAATGAACTAATTGTTTTACTCGGTGTTTTGAAGATTCTGGTGGTGGAGGTAATCAGTTCCGCCCAATAAGCTATTAAGTTGGTGTAAGAGACGGTAATTAAGTTTGGTTTTGGCTTTGTTTACATCTAACTGGACACTGTGATTGGATGATTTATTGTGGATTCGAGAGGGTAGGGGCTAGATTTTGCTATAATTAGTCATTCATAGATAGCTGTGGAAAAACGCTTTTATCTGCTCCATGATGGGTGCTTTTCCTTCAGCGAAGATGCTTGTTATAGGTGTAGTCTGATGGGGAAAAGTCGGTTCCCTGCCTATACAGCTGATGAAGGTTCTATGGTGTGACCAAATTGGCCAGATTCTTGTTCTGTGATTTGGGTTCTTTGCAGAACTGAGAAATTTTGGTCCTGTATTGAGGTTATTTAGCCCAGGCTTAATTTATTTTAAGTCTGAGAAGCTGCATTGAGATTTTATAAACTGGCG_-_ATGATAAAGAAGAAGCAGAAGGATTTTGATAAGGTTGTATGGGATCAGATGGCTGGAAGGAAAGTCCACCCATCTGCTGGTATGCTTTCCCGCATTGGGGGTTGGGCTAGTAAGGTTTTTAGATCGCTCATTGTGTTGATCTGTATATCTTACTTCATATATCTTATGAATCTTATGCTAAGCAATTACCCATGTTGGCAACCCATCATAAGTTGCAGAACCGAGTGTACTCAGTCGGCTAGTTCTAGTTCTAGTTCTAGTACTAGTACTACTAACAGTGTGGCAGTTTTAGCCGTGGAGAATACTACGAAGGTTGTCAATGCCGACGAAGCTGGTATTCACAGGCAGGCGCTTCAAGCTATTCCTGATTCTACAGGAACTGACTTGAAGCACATAGTGTTTGGGATTGCCGCCTCTGCTAGGTTATGGGACAAGAGAAAAGAGTACATAAAGTTGTGGTGGAGGCCGAAGCAGATGAGGGGGTTTATTTGGTTAGAGAAGCCTGTGAAAACTGGCAAGGGTGACAATTCTTCCCTGCCCCCATGGAGAATATCAGCCAATACCTCCCATTTTAAGTACACCAACAAGCAGGGACACAGATCTGCCATTAGAATTTCCCGCATTGTCTCTGAGACTTTCAGAATGGGTCTTCCTGATGTGCATTGGTTTGTCATGGGGGATGATGATACTGTCTTTATTCCTGATAATCTGGTGACGGTTCTGTCCAAGTATGATCACACGAAACCTTACTACATTGGCAGTTTATCAGAGTCTCATTTGCAGAATATATTCTTTTCTTATAGTATGGCTTATGGGGGAGGCGGATTTGCCATTAGCTATCCTCTGGCAAAGGCGTTGGAGACTATGCAAGATAAGTGTATTCAGAGGTATCCTGGTTTGTATGGAAGCGATGATAGAATxxxACAGGCCTGCATGTCTGAGTTGGGCGTGCCGCTTACAAAGGAGCCTGGCTTTCATCAGTATGATGTATATGGTAATTTATTCGGATTACTGGCAGCTCATCCTTTAACTCCCTTGGTGTCACTTCACCATTTGGATGTTGTGGAACCTATATTCCCAAACATGACCAGAGTAAAGGCACTGCAGCACTTAAGCAAAGCTGTAAAATTAGATTCAGCTGGAATAATGCAACAGTCTATTTGTTATGATAAAAAGCGTGAATGGTCTATATCAGTCTCATGGGGTTATGCAGTTCAGATCTACAGAACGATATTTTCCCCTCGTGAGTTGGAGATGCCCTCCAGGACGTTCTTGAATTGGTACAGAAGAGCAGACTTTACAGCATATGCCTTCAATACCAGGCCCGTGACAAGACATCCATGCCAGAAACCCTTCATATTTTATTGA___AGGATGTTCGCCATGATAATGCGAGTGGACAAATCATAAGCAATTATGCTAGACAGCAAGTAAAGCCTCCTGGATGCAGGTGGAGAATACCTTCCCCAGAGATCATAAATTTGATTCGGGTTGTGAAGAAACCTGATACATTATTATGGCAGTCACCACGGCGAAACTGCTGCAGAGTGGTAGCATCCAAAAAGTCCAGCCTTGATATTGAAGTGGGTGCCTGCCGGGAAGGTGAGATCATTGGTGTATCATAGCACATCTGCTGTACAAGAATGTCTTTATCAGAAGGCCTATGCACGCATTTTGGGAGTAGATCAGCCAGCTTC

Fln Alignment:

Contig6331___IVLICISYFIYLMNLMLSNYPCWQPIISCRTECTQXXXXXXXXXXXXXXXXXXXXXENTTKVVNADEAGIHRQALQAIPDSTGTDLKHIVFGIAASARLWDKRKEYIKLWWRPKQMRGFIWLEKPVKTGKGDNSSLPPWRISANTSHFKYTNKQGHRSAIRISRIVSETFRMGLPDVHWFVMGDDDTVFIPDNLVTVLSKYDHTKPYYIGSLSESHLQNIFFSYSMAYGGGGFAISYPLAKALETMQDKCIQRYPGLYGSDDRMxQACMSELGVPLTKEPGFHQYDVYGNLFGLLAAHPLTPLVSLHHLDVVEPIFPNMTRVKALQHLSKAVKLDSAGIMQQSICYDKKREWSISVSWGYAVQIYRTIFSPRELEMPSRTFLNWYRRADFTAYAFNTRPVTRHPCQKPFIFY
B8LNL2_______________VILCC--WLFYVSFLVLSTHPCWQS-----SDCLAV-------------------------LVTGDNAGRKLNSVEA----TATSLDHIVFGIAASAHKWKQRKPYIDLWWEPGRTRGFVWLDRPVNETSPPG---PPIRISEDTSQFNYTHPQGMRSAIRISRIVSEIFRLGLPDVRWFVMGDDDTLFLPQNLVRVLAKYDHNQYYYIGSNSESTDQNAMHSYGMAYGGGGFAISYPLARDLAQVQDDCLHRYPHLYGSDQRVxQACLAELGVPLTKEPGFHQFDIHGNLFGLLAAHPIAPLVSLHHLESVAPVFPNMTRVGALRHLMEAARVDPDRALQQSICYDARRKWTVSVSWGYCVQVLDRIELPRVLEFPLQTFVAWGHSMK-TPFLFNTRPVPRNPCQRATVLF

SNPs (tot: 164)

Position	A %	C %	G %	T %	Probability
477	83	16			0.994512
478			83	16	0.994512
482		12	87		0.992554
549	87	12			0.992554
550			12	87	0.992554
552		12		87	0.992554
554			87	12	0.992554
556	12			87	0.992554
557		12		87	0.992554
561			12	87	0.992554
564	87			12	0.992554
566	83	16			0.994512
570		16	83		0.994512
661	75			25	0.996473
662		25		75	0.996473
664	75			25	0.996473
665			25	75	0.996473
667	75	25			0.996473
668	75	25			0.996473
669			75	25	0.996473
670	75			25	0.996473
671	75		25		0.996473
673	75	25			0.996473
674	75			25	0.996473
680			75	25	0.996473
681	75	25			0.996473
714			75	25	0.996473
794		20		80	0.995493
797		20	80		0.995493
798			20	80	0.995493
802		20		80	0.995493
807	80		20		0.995493
808			20	80	0.995493
809	80	20			0.995493
811		80	20		0.995493
813		20		80	0.995493
817		20		80	0.995493
818	20	80			0.995493
953	66	33			0.997452
1124		28	71		1.0
1125	85			14	0.993533
1127	14		85		0.993533
1130			85	14	0.993533
1131	85			14	0.993533
1132	85		14		0.993533
1135	85			14	0.993533
1139		14	85		0.993533
1142		14	85		0.993533
1143			85	14	0.993533
1144		14	85		0.993533
1145	14		85		0.993533
1165		90	10		0.90298
1172	90	10			0.90298
1173	90	10			0.90298
1174		90		10	0.90298
1179	90		10		0.90298
1180	90			10	0.90298
1181		11	88		0.923564
1182	11		88		0.923564
1183		11	88		0.923564
1185		11	88		0.923564
1269	90		10		0.90298
1271			10	90	0.90298
1272	90			10	0.90298
1275	90	10			0.90298
1276		10		90	0.90298
1281		90	10		0.90298
1284	90			10	0.90298
1285			10	90	0.90298
1287		10	90		0.90298
1288	10			90	0.90298
1292	10			90	0.90298
1302	90	10			0.90298
1304	90			10	0.90298
1305	90	10			0.90298
1306			10	90	0.90298
1307			90	10	0.90298
1318	81	18			0.999994
1537	80	20			0.995493
1542	80		20		0.995493
1543	20			80	0.995493
1544		20		80	0.995493
1546	80			20	0.995493
1547			80	20	0.995493
1548	80		20		0.995493
1550		20	80		0.995493
1552	80		20		0.995493
1558		25	75		0.996473
1559	25			75	0.996473
1563		25		75	0.996473
1573	75			25	0.996473
1574		25		75	0.996473
1576	75			25	0.996473
1577			25	75	0.996473
1579		25	75		0.996473
1580	75	25			0.996473
1581	75			25	0.996473
1584	75		25		0.996473
1586	75			25	0.996473
1588			75	25	0.996473
1593		25	75		0.996473
1594	25	75			0.996473
1611		20		80	0.995493
1613		80		20	0.995493
1618			33	66	0.997452
1621	66	33			0.997452
1622	66			33	0.997452
1623	66			33	0.997452
1626	66			33	0.997452
1631		33	66		0.997452
1632	33		66		0.997452
1687		25		75	0.996473
1688			25	75	0.996473
1690	75	25			0.996473
1691	75		25		0.996473
1692		75		25	0.996473
1693			25	75	0.996473
1694	25	75			0.996473
1707			12	87	0.992554
1710	87		12		0.992554
1711		87		12	0.992554
1712		87	12		0.992554
1714		12		87	0.992554
1719	87	12			0.992554
1723	12			87	0.992554
1724		12	87		0.992554
1726		12	87		0.992554
1727	12		87		0.992554
2117	75	25			0.996473
2118		20	20	60	0.99255
2119	60		20	20	0.99255
2120		80		20	0.995493
2121		80	20		0.995493
2122			20	80	0.995493
2124	20			80	0.995493
2125		80	20		0.995493
2126	20	80			0.995493
2127		80		20	0.995493
2128		80		20	0.995493
2129	60		20	20	0.99255
2130			80	20	0.995493
2131	60	20	20		0.99255
2132			80	20	0.995493
2133	60	20	20		0.99255
2136	80		20		0.995493
2137		20		80	0.995493
2138	60	20		20	0.99255
2139	80	20			0.995493
2140	80	20			0.995493
2142		20		80	0.995493
2143	20			80	0.995493
2167	66	22		11	0.999999
2168			33	66	1.0
2169	77			22	0.999999
2171	66	11	22		0.999999
2173			22	77	0.999999
2175		22	11	66	0.999999
2176		22	11	66	0.999999
2177	66	11		22	0.999999
2180			88	11	0.923564
2182	77			22	0.999999
2183		11	88		0.923564
2187		88	11		0.923564
2192			77	22	0.999999

SSRs (tot: 1)

Start position	End position	Sequence	Length
895	917	CTAGTT CTAGTT CTAGTT CTAGT	23

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain