SustainPineDB

Assembly Name: uagpf v2.0

UniGene Name: uagpf_v2_unigene528

Length: 1252 nt

UniGene Fasta

Ace file of the UniGene uagpf_v2_unigene528

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene8419	23/27
SustainPine v2.0	sp_v2.0_unigene31247	4/27

Source	Descriptions	Term	Type	Identity
AutoFact	sphingoid base hydroxylase 1 [Arabidopsis thaliana] gb\|AAL50102.1\| At1g69640/F24J1.22 [Arabidopsis thaliana] gb\|AAN28857.1\| At1g69640/F24J1.22 [Arabidopsis thaliana] gb\|AEE34958.1\| sphingoid base hydroxylase 1 [Arabidopsis thaliana]	-	-	66%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	93%
Blast2go	sur2 hydroxylase	-	-	84%

Source	ECs	Term	Type	e value	Identity
Blast2go	Methylsterol monooxygenase.	EC:1.14.13.72	-	0.0	84%

Source	KEGGs	Term	Identity
Blast2go	Steroid biosynthesis	00100	84%
Blast2go	Biosynthesis of alkaloids derived from terpenoid and polyketide	01066	84%
Blast2go	Metabolic pathways	01100	84%

Source	GOs	Term	Type	Identity
Blast2go	sphingoid biosynthetic process	GO:0046520	Biological Process	84%
Blast2go	fatty acid biosynthetic process	GO:0006633	Biological Process	84%
Blast2go	oxidation-reduction process	GO:0055114	Biological Process	84%
Blast2go	photomorphogenesis	GO:0009640	Biological Process	84%
Blast2go	C-4 methylsterol oxidase activity	GO:0000254	Molecular Function	84%
Blast2go	sphingosine hydroxylase activity	GO:0000170	Molecular Function	84%
Blast2go	iron ion binding	GO:0005506	Molecular Function	84%
Blast2go	integral to membrane	GO:0016021	Cellular Component	84%
Blast2go	cytoplasmic membrane-bounded vesicle	GO:0016023	Cellular Component	84%
Blast2go	endoplasmic reticulum	GO:0005783	Cellular Component	84%
Blast2go	Golgi apparatus	GO:0005794	Cellular Component	84%

Source	InterPros	Term	Type	e value	Identity
Sma3	Fatty acid hydroxylase	IPR006694	-	0.0	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: A9P011

Fln msg: your sequence is shorter than subject: 227 - 258

Fln protein: RDLGCHSGYRMHTLKEEKLKNLVSRSTVVKGVLLQQSVQAVVALSLFAVTTVEAGKISQPSLMVQARQFLIAMFVMDTWQYFLHRYMHHNKFLYRYIHSQHHKLIVPYAFGALYNHPLEGLLLDTIGGALSFLFSGMTPRTSIFFFSFSTIKTVDDHCGLWLPGNPFHIIFQNNTAYHDIHHQLYGAKYNFEQPFFVMWDKILGTYMPYTIQARPDGGFEARPDKS*

Protein Length: 228

Fln nts: TCGTGACCTTGGCTGTCACTCAGGCTACAGAATGCATACTCTTAAGGAAGAGAAATTGAAGAATTTGGTGTCAAGGAGTACTGTTGTGAAGGGTGTGTTGCTTCAGCAGTCTGTTCAGGCTGTGGTTGCCTTATCTTTATTTGCGGTTACCACAGTTGAAGCTGGCAAAATTTCACAACCTTCACTTATGGTACAGGCAAGACAATTTTTAATAGCAATGTTTGTCATGGACACCTGGCAATACTTTCTCCACCGTTATATGCACCACAACAAGTTCTTGTATCGTTACATTCATTCACAGCATCATAAGCTTATAGTGCCATATGCTTTTGGAGCACTGTATAACCATCCACTGGAAGGTTTGCTTCTAGACACCATAGGTGGTGCTCTGTCATTTCTTTTCTCAGGGATGACTCCTCGAACATCAATATTCTTTTTCTCCTTTTCAACAATTAAAACAGTTGATGATCACTGTGGCCTTTGGCTCCCTGGGAATCCATTTCATATCATCTTTCAGAATAATACAGCATATCATGACATTCATCATCAGTTGTATGGGGCGAAGTACAACTTTGAACAGCCTTTCTTTGTAATGTGGGATAAAATTCTAGGGACTTATATGCCTTATACGATTCAAGCACGACCAGACGGTGGATTTGAAGCTCGACCAGACAAAAGTTAA___TATTTTTGGAACACTATATAGTATATACTATATAGTATTAATACATCTTGTAATGTACATCGGTCTGTACTCTTCGTTGCTGACAGTGGCAAAATATCTGATATTTTCACTTGTCAGGCTAAGCAACAATCTCTACTTTCATATTGTAACATATTTCAAATCAATACATAACTTAAAGCCCTTATGTTTAGCTTTTAAGGCGTTGGCAAGAGCATGTTTTGACAATTGGACAACTTAAATGCGAGTGCAGAGCATAATGTCATTTGGTAAATGCAAGATGGTTTTATTTTAATTTCTGGTTATTATTACCAGGAACCAAGGTGCTTATCCCCAGTTGTTGATTTGCCGACAAAATGTTTAGTTAATACTTTTATGCCCCGGATGCAGTGGAAGAAAGACTATTACTTTTATGCCCCGGATGCAGTGGAAGAAAGACTATTAAAGAGGTAGTCGACTGTGAAAAAGGATCAAACAGATGGTTAGCCCCTTTGAATATCTATAGAACTTGGAAAGGTCTGTAAAACTTAATTGGTATAATAGGCCATGTCTTGTTTATTTCCGCGGCTTCGC

Fln Alignment:

Contig528___GYRMHTLKEEKLKNLVSRSTVVKGXXXXXXXXXXXXXXXFAVT--TVEAGKISQPSLMVQARQFLIAMFVMDTWQYFLHRYMHHNKFLYRYIHSQHHKLIVPYAFGALYNHPLEGLLLDTIGGALSFLFSGMTPRXXXXXXXXXXXKTVDDHCGLWLPGNPFHIIFQNNTAYHDIHHQLYGAKYNFEQPFFVMWDKILGTYMPYTIQARPDGGFEARPDKS
A9P011______________GYRVHTLKEEKLKNLVSRSTVVKGVLLQQSIQSVVALSLFAITGHTVEAGTTSQPSLMVQARQFLIAMLVMDTWQYFLHRYMHHNKFMYRYIHSQHHQLIVPYAFGALYNHPLEGLLLDTIGGAMSFLFSGMTPRTSIFFFSFSTIKTVDDHCGLWLPGNPFHIFFQNNTAYHDIHHQLYGAKYNFEQPFFVMWDKILGTYMPYTIQKRPDGGLEARPDKS

SNPs (tot: 31)

Position	A %	C %	G %	T %	Probability
303	66		33		1.0
306	66		33		1.0
308	66		33		1.0
310		33	66		1.0
315	66		33		1.0
316		33		66	1.0
317	66			33	1.0
321	33		66		1.0
383	75			25	1.0
388		25	75		1.0
394	25			75	1.0
395		28	71		1.0
493		66	25	8	1.0
496	16		83		0.999991
497		25	66	8	1.0
498	66	16	8	8	0.999991
499	75		8	16	0.99999
501		75	16		0.99999
502		83	16		0.999991
503	75	16			0.99999
505			16	83	0.999991
510	75	16	8		0.99999
888		20	80		0.995493
894	80			20	0.995493
895	80		20		0.995493
896			80	20	0.995493
897	80	20			0.995493
898	20		80		0.995493
900	80			20	0.995493
901		20		80	0.995493
902	20		80		0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain