SustainPineDB

Assembly Name: biogeco3 v1.0

UniGene Name: biog3_v1.0_unigene13088

Length: 2288 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>biog3_v1.0_unigene13088
ATGGGTGGACTGGTGGTGAGATTGGATTCGACACTTACTTTTCAATGGCACGCGGAAATGCTAAGCTTCCTGCTATGGAGATGACCAAGTGGTTTGACACTAACTACCACTACATCGTTCCAGAGCTTGGACCAGAAATTAAATTCAAGTACTCTTCACACAAAGCTGTGCATGAATACAAAGAGGCTAAAGAGATTGGTATTGAGACTGTACCTGTCATCGTAGGTCCTGTGTCATACTTGTTGTTATCAAAGCCTGCAAAAGGTGTTCCTAAAACATTCAACCTTCTTTCTCTGCTTGATTCAATTCTGCCGGTCTACAAAGAGGTCTTAGGTGAGTTGAAGGAGGCTGGGGCAACATGGATACAGTTTGATGAACCCTCGCTTGTAATGGACCTTGAGGCTCACCACCTGAACGCTTTCTCTAGAGCTTATTCTTTCTTAGAAGAAGTACTTGTTGGGACCAAATTGCTTGTAGAAACATACTTTGCAGATATTCCTGTTGAGACATATAGGACCATCATCCAGTTGAAGGCTATATCTGCTATTGGGTTAGACCTAGTACGTGGAACTAAAACACTCAACCTTGTCAAAACTGTTGGCTTTCCGGGAGACAAATACCTTTTCGACTGGAGTTATTGATGGGAGAAACATCTGGGCTAATGATTTGGCAGCATCGTTTAACATTTTAAAGGATCTCGAAAAGAGTGTTGTTAAAGACAAAATAGTTGTCTCCACCTCCTGCTCTCTTCTCCATACAGCTGTGGATTTAGTAAATGAGACAAAGCTTGATAATGAGATTAAGTCCTGGCTTGCATTTGCTGCACAAAAAGTACTTGAAGTAGTAGCTCTGGCAAAAGCATTGATTGGTCAAGCAGATCAGGCATATTTCACTGCCAACGCAGCTGCTATATCTTCAAGAAAGACCTCTCCTAGAGTGAACAATAAGGCAGTGCAAAAAGCTACTGAAGCACTAAAAGGCTCTGATCACAGGCGAGCTACACCTGTAGTTGTTCGTTTAGATACGCAGCAGAAAAAGTTGAACCTCCCAATTCTTCCAACGACCACAATTGGGTCCTTTCCTCAGACAGTAAAACTTCGCAGAGTTCGCCGTGAATACAAATCAAACAAGATTTCAGAAGCGGATTACATTCAGTCCATTCAGCAGGAAATCGCAAAAGTGGTTAAACTGCAGGAAGATCTGGACATCGATGTTCTAGTGCATGGTGAACCTGAGAGAAACGACATGGTAGAATACTTTGGGGAACAGCTTCAGGGATTTGCATTCACAGTTAATGGCTGGGTACAGTCTTATGGTTCTCGTTGTGTGAAGCCACCGATTATTTATGGAGATGTCAGCCGCCCAAAACCAATGACTGTGTTTTGGTCAAAGTATGCCCAGAGTTTGACTGCCCGCCCTATGAAGGGAATGCTTACAGGTCCTGTGACAATTCTGAATTGGTCCTTTGTAAGAGATGATCAGCCCAGGTCAGAAACCAGCATGCAAATTGCACTAGCTATCAAAGACGAGGTTGAAGATTTGGAGGTAGCTGGTATTCAGGTGATCCAAATCGATGAGGCAGCCCTAAGAGAAGGACTACCTTTGCGTAAAGCAGAACAGGCTCACTACCTGGAATGGGCTGTTCATGGTTTCCGTATCACCAATTGTGGTGTAAAGGATACTACTCAGATCCATACCCACATGTGTTACTCCAACTTCAACGACATTATTCATTCCATTATTAATATGGATGCCGATGTCATTACCATTGAGAATTCCCGCTCCGATGAGAAATTATTGAGAGTTTTCCAGGAGGGCGTGAAATATGGGGCTGGAATCGGTCCTGGGGTTTATGATATACACTCTCCTCGCATTCCAGACACGGAAGAAATCGCAGACCGTATCCGCAAGATACTTGCTGTGCTGGAGACAAACATCCTGTGGGTAAATCCAGATTGTGGTTTGAAAACCAGGAAGTATGATGAAGTTGTTCCTGCATTGACAAACATGGTTGCATCTGCCAAGTTGCTGCGAAGTGAGCTGGCCTCTGCAAAATGATCAAGAAAACAATTTAACATCAGCATTTTAGTGCAATAAAGGTGTGGAGGAATAATTTTGGGTTGCTTATGTGAAGGGGTCAGATGATCTATTGTTTAGAAGTATATCAGTGGAAGTTCCACATAAGTAGCAGGTTTCATTCTCAGTGCATATCATATGGCTGTACCTGAGAATCACCTAAGTGCACATCACGAAGATTGAGCCCACTCTGGCAAAAAATGTAGAGTTTC

Ace file of the UniGene biog3_v1.0_unigene13088 (antisense)

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene6571	2/49
SustainPine v2.0	sp_v2.0_unigene16429	10/49
SustainPine v2.0	sp_v2.0_unigene24872	35/49

Source	Descriptions	Term	Type	Identity
AutoFact	Vitamin-b12 independent methionine synthase, 5-methyltetrahydropteroyltriglutamate-homocysteine n=2 Tax=Populus trichocarpa RepID=B9I615_POPTR	-	-	80%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	94%
Blast2go	5-methyltetrahydropteroyltriglutamate-homocysteine expressed	-	-	90%

Source	InterPros	Term	Type	Identity
Sma3	Cobalamin (vitamin B12)-independent methionine synthase MetE, N-terminal	IPR013215	-	-
Sma3	Methionine synthase, vitamin-B12 independent	IPR002629	-	-
Sma3	Cobalamin-independent methionine synthase	IPR006276	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LKF4

Fln msg: Overlapping hits, possible frame ERROR between 626 and 574, your sequence is shorter than subject: 686 - 766

Fln protein: GWTGGEIGFDTYFSMARGNAKLPAMEMTKWFDTNYHYIVPELGPEIKFKYSSHKAVHEYKEAKEIGIETVPVIVGPVSYLLLSKPAKGVPKTFNLLSLLDSILPVYKEVLGELKEAGATWIQFDEPSLVMDLEAHHLNAFSRAYSFLEEVLVGTKLLVETYFADIPVETYRTIIQLKAISAIGLDLVRGTxxxxxxxxxxxxxxxxxxxTGVIDGRNIWANDLAASFNILKDLEKSVVKDKIVVSTSCSLLHTAVDLVNETKLDNEIKSWLAFAAQKVLEVVALAKALIGQADQAYFTANAAAISSRKTSPRVNNKAVQKATEALKGSDHRRATPVVVRLDTQQKKLNLPILPTTTIGSFPQTVKLRRVRREYKSNKISEADYIQSIQQEIAKVVKLQEDLDIDVLVHGEPERNDMVEYFGEQLQGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPMTVFWSKYAQSLTARPMKGMLTGPVTILNWSFVRDDQPRSETSMQIALAIKDEVEDLEVAGIQVIQIDEAALREGLPLRKAEQAHYLEWAVHGFRITNCGVKDTTQIHTHMCYSNFNDIIHSIINMDADVITIENSRSDEKLLRVFQEGVKYGAGIGPGVYDIHSPRIPDTEEIADRIRKILAVLETNILWVNPDCGLKTRKYDEVVPALTNMVASAKLLRSELASAK*

Protein Length: 687

Fln nts: ATGGGTGGACTGGTGGTGAGATTGGATTCGACACTTACTTTTCAATGGCACGCGGAAATGCTAAGCTTCCTGCTATGGAGATGACCAAGTGGTTTGACACTAACTACCACTACATCGTTCCAGAGCTTGGACCAGAAATTAAATTCAAGTACTCTTCACACAAAGCTGTGCATGAATACAAAGAGGCTAAAGAGATTGGTATTGAGACTGTACCTGTCATCGTAGGTCCTGTGTCATACTTGTTGTTATCAAAGCCTGCAAAAGGTGTTCCTAAAACATTCAACCTTCTTTCTCTGCTTGATTCAATTCTGCCGGTCTACAAAGAGGTCTTAGGTGAGTTGAAGGAGGCTGGGGCAACATGGATACAGTTTGATGAACCCTCGCTTGTAATGGACCTTGAGGCTCACCACCTGAACGCTTTCTCTAGAGCTTATTCTTTCTTAGAAGAAGTACTTGTTGGGACCAAATTGCTTGTAGAAACATACTTTGCAGATATTCCTGTTGAGACATATAGGACCATCATCCAGTTGAAGGCTATATCTGCTATTGGGTTAGACCTAGTACGTGGAACTxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxACTGGAGTTATTGATGGGAGAAACATCTGGGCTAATGATTTGGCAGCATCGTTTAACATTTTAAAGGATCTCGAAAAGAGTGTTGTTAAAGACAAAATAGTTGTCTCCACCTCCTGCTCTCTTCTCCATACAGCTGTGGATTTAGTAAATGAGACAAAGCTTGATAATGAGATTAAGTCCTGGCTTGCATTTGCTGCACAAAAAGTACTTGAAGTAGTAGCTCTGGCAAAAGCATTGATTGGTCAAGCAGATCAGGCATATTTCACTGCCAACGCAGCTGCTATATCTTCAAGAAAGACCTCTCCTAGAGTGAACAATAAGGCAGTGCAAAAAGCTACTGAAGCACTAAAAGGCTCTGATCACAGGCGAGCTACACCTGTAGTTGTTCGTTTAGATACGCAGCAGAAAAAGTTGAACCTCCCAATTCTTCCAACGACCACAATTGGGTCCTTTCCTCAGACAGTAAAACTTCGCAGAGTTCGCCGTGAATACAAATCAAACAAGATTTCAGAAGCGGATTACATTCAGTCCATTCAGCAGGAAATCGCAAAAGTGGTTAAACTGCAGGAAGATCTGGACATCGATGTTCTAGTGCATGGTGAACCTGAGAGAAACGACATGGTAGAATACTTTGGGGAACAGCTTCAGGGATTTGCATTCACAGTTAATGGCTGGGTACAGTCTTATGGTTCTCGTTGTGTGAAGCCACCGATTATTTATGGAGATGTCAGCCGCCCAAAACCAATGACTGTGTTTTGGTCAAAGTATGCCCAGAGTTTGACTGCCCGCCCTATGAAGGGAATGCTTACAGGTCCTGTGACAATTCTGAATTGGTCCTTTGTAAGAGATGATCAGCCCAGGTCAGAAACCAGCATGCAAATTGCACTAGCTATCAAAGACGAGGTTGAAGATTTGGAGGTAGCTGGTATTCAGGTGATCCAAATCGATGAGGCAGCCCTAAGAGAAGGACTACCTTTGCGTAAAGCAGAACAGGCTCACTACCTGGAATGGGCTGTTCATGGTTTCCGTATCACCAATTGTGGTGTAAAGGATACTACTCAGATCCATACCCACATGTGTTACTCCAACTTCAACGACATTATTCATTCCATTATTAATATGGATGCCGATGTCATTACCATTGAGAATTCCCGCTCCGATGAGAAATTATTGAGAGTTTTCCAGGAGGGCGTGAAATATGGGGCTGGAATCGGTCCTGGGGTTTATGATATACACTCTCCTCGCATTCCAGACACGGAAGAAATCGCAGACCGTATCCGCAAGATACTTGCTGTGCTGGAGACAAACATCCTGTGGGTAAATCCAGATTGTGGTTTGAAAACCAGGAAGTATGATGAAGTTGTTCCTGCATTGACAAACATGGTTGCATCTGCCAAGTTGCTGCGAAGTGAGCTGGCCTCTGCAAAATGATCAAGAAAACAATTTAACATCAGCATTTTAGTGCAATAAAGGTGTGGAGGAATAATTTTGGGTTGCTTATGTGAAGGGGTCAGATGATCTATTGTTTAGAAGTATATCAGTGGAAGTTCCACATAAGTAGCAGGTTTCATTCTCAGTGCATATCATATGGCTGTACCTGAGAATCACCTAAGTGCACATCACGAAGATTGAGCCCACTCTGGCAAAAAATGTAGAGTTTC

Fln Alignment:

biogeco3_mira_c111___GWTGGEIGFDTYFSMARGNAKLPAMEMTKWFDTNYHYIVPELGPEIKFKYSSHKAVHEYKEAKEIGIETVPVIVGPVSYLLLSKPAKGVPKTFNLLSLLDSILPVYKEVLGELKEAGATWIQFDEPSLVMDLEAHHLNAFSRAYSFLEEVLVGTKLLVETYFADIPVETYRTIIQLKAISAIGLDLVRGTxxxxxxxxxxxxxxxxxxTGVIDGRNIWANDLAASFNILKDLEKSVVKDKIVVSTSCSLLHTAVDLVNETKLDNEIKSWLAFAAQKVLEVVALAKALIGQADQAYFTANAAAISSRKTSPRVNNKAVQKATEALKGSDHRRATPVVVRLDTQQKKLNLPILPTTTIGSFPQTVKLRRVRREYKSNKISEADYIQSIQQEIAKVVKLQEDLDIDVLVHGEPERNDMVEYFGEQLQGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPMTVFWSKYAQSLTARPMKGMLTGPVTILNWSFVRDDQPRSETSMQIALAIKDEVEDLEVAGIQVIQIDEAALREGLPLRKAEQAHYLEWAVHGFRITNCGVKDTTQIHTHMCYSNFNDIIHSIINMDADVITIENSRSDEKLLRVFQEGVKYGAGIGPGVYDIHSPRIPDTEEIADRIRKILAVLETNILWVNPDCGLKTRKYDEVVPALTNMVASAKLLRSELASAK
B8LKF4__________________GWTGGEIGFDTYFSMARGNAKLPAMEMTKWFDTNYHYIVPELGPEIKFKYSSHKAVDEYKEAKKIGIETVPVIIGPVSYLLLSKPAKGVPKTFDLLSLLDAILPVYKEVLGELKQAGATWIQFDEPALVMDLEAHHLDAFSKAYSFLEEVLVGIKLLVETYFADIPVETYRTIIQLKAISAIGLDLIRGTxxxxxxxxxxxxxxxxxxAGVIDGRNIWANDLAASFSILKDLETIVGKGKIVVSTSCSLLHTAVDLVNETKLDNEIKSWLAFAAQKVLEVVALAKALIGQADQPYFAANAAAISSRKTSPRVNNEAVQKAAEALKGSDHRRATPVIARLDEQQKKLNLPILPTTTIGSFPQTVELRRVRREYKSNKISEADYIQSIQQEIAKVVKMQEDLDIDVLVHGEPERNDMVEYFGEQLQGFAFTVNGWVQSYGSRCVKPPVIYGDVSRPKPMTVFWSKYAQSLTARPMKGMLTGPVTILNWSFVRDDQPRSETSMQIALAIKEEVEDLEASGIQVIQIDEAALREGLPLRKAEQAHYLEWAVHGFRITNCGVKDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLRVFQEGVKYGAGIGPGVYDIHSPRIPDTEEIADRIRKILAVLETNILWVNPDCGLKTRKYNEVVPALTNMVASAKLLRTELASAK

SNPs (tot: 36)

Position	A %	C %	G %	T %	Probability
1771	80		20		0.995493
1772			80	20	0.995493
1792	20		80		0.995493
1795			80	20	0.995493
1815	16	83			0.994512
1817			83	16	0.994512
1819	83			16	0.994512
1825	33			66	1.0
1861	60	40			1.0
1863	50		50		1.0
1864		40		60	1.0
1889		60		40	1.0
1900	50		50		1.0
1934	40		60		1.0
1960	16		83		0.994512
1995		50		50	1.0
1998	50		50		1.0
2001		25	75		0.996473
2003		75		25	0.996473
2004		75		25	0.996473
2007	25	75			0.996473
2011	75			25	0.996473
2014		50		50	1.0
2037		33		66	0.997452
2038	33		66		0.997452
2041		33		66	0.997452
2057		33		66	0.997452
2099		75		25	0.996473
2100	25		75		0.996473
2109	12			87	0.992554
2112		87	12		0.992554
2113	87			12	0.992554
2137	12	75		12	0.975373
2141	12	87			0.992557
2153		33	66		1.0
2197	75	25			0.996479

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain