SustainPineDB

Assembly Name: biogeco3 v1.0

UniGene Name: biog3_v1.0_unigene10994

Length: 845 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene biog3_v1.0_unigene10994 (antisense)

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene1835	13/13

Source	Descriptions	Term	Type	Identity
AutoFact	dihydrolipoamide S-acetyltransferase precursor [Arabidopsis thaliana]	-	-	56%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	91%
Blast2go	dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex	-	-	71%

Source	ECs	Term	Type	e value	Identity
Blast2go	Dihydrolipoyllysine-residue acetyltransferase.	EC:2.3.1.12	-	0.0	71%

Source	KEGGs	Term	Identity
Blast2go	Glycolysis / Gluconeogenesis	00010	71%
Blast2go	Citrate cycle (TCA cycle)	00020	71%
Blast2go	Pyruvate metabolism	00620	71%
Blast2go	Biosynthesis of phenylpropanoids	01061	71%
Blast2go	Biosynthesis of terpenoids and steroids	01062	71%
Blast2go	Biosynthesis of alkaloids derived from shikimate pathway	01063	71%
Blast2go	Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid	01064	71%
Blast2go	Biosynthesis of alkaloids derived from histidine and purine	01065	71%
Blast2go	Biosynthesis of alkaloids derived from terpenoid and polyketide	01066	71%
Blast2go	Biosynthesis of plant hormones	01070	71%
Blast2go	Metabolic pathways	01100	71%
Blast2go	Biosynthesis of secondary metabolites	01110	71%

Source	GOs	Term	Type	Identity
Blast2go	metabolic process	GO:0008152	Biological Process	71%
Blast2go	dihydrolipoyllysine-residue acetyltransferase activity	GO:0004742	Molecular Function	71%
Blast2go	ATP binding	GO:0005524	Molecular Function	71%
Blast2go	mitochondrion	GO:0005739	Cellular Component	71%

Source	InterPros	Term	Type	Identity
Sma3	Biotin/lipoyl attachment	IPR000089	-	-
Sma3	2-oxoacid dehydrogenase acyltransferase, catalytic domain	IPR001078	-	-
Sma3	2-oxo acid dehydrogenase, lipoyl-binding site	IPR003016	-	-
Sma3	E3 binding	IPR004167	-	-
Sma3	Dihydrolipoamide acetyltransferase, long form	IPR006257	-	-

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LPX9

Fln msg: STOP codon was not found. Distance to subject end: 16 aas, your sequence is shorter than subject: 281 - 529

Fln protein: MYKPSESQKTEKTFLASTDPVSLQSPLPLQSSGLYEDLQNSQIRKIIAKRLWESKHGTPHLYLSADVMLDPVLNFRKELQEKHGLKISVNDIVIKFAALALKAVPEANAYWSDEKGEVVLCDSIDISIAVATEKGLMTPILRKADQKSLSVISAEVKELANKARVGKLTPSEFQGGTFSISNLGMFPVDRFCAIINPPQACILAVGRGNKVVKWEEDSSGQGKAHSVTQMNLSLSADHRVFDYDIGGKLWMPCPQTSWKPTDFSYEIPYELQPVIKLQFSY

Protein Length: 282

Fln nts: AATGTACAAGCCGTCTGAATCGCAAAAAACTGAGAAGACATTCTTAGCCTCAACAGATCCTGTATCTTTGCAATCTCCCTTGCCGCTGCAGTCATCTGGGCTTTATGAAGACTTACAAAACAGTCAAATTCGCAAGATCATCGCAAAACGTCTATGGGAATCAAAGCATGGGACTCCACATCTTTATTTATCTGCAGATGTAATGCTGGATCCTGTGCTGAATTTTAGAAAGGAACTTCAAGAAAAGCATGGACTGAAGATTTCTGTAAATGATATTGTCATCAAGTTTGCGGCACTTGCTTTGAAGGCCGTTCCTGAAGCAAATGCATATTGGAGTGATGAAAAGGGAGAGGTAGTTTTATGCGATTCAATTGACATCTCCATAGCAGTTGCCACTGAAAAGGGGTTGATGACACCAATTTTGCGGAAAGCAGACCAGAAGTCTCTGTCAGTGATCTCGGCTGAGGTGAAGGAACTGGCAAATAAAGCAAGGGTTGGAAAGCTGACCCCTAGTGAATTTCAAGGTGGAACTTTCAGCATTTCTAACCTGGGAATGTTCCCTGTAGACCGTTTCTGTGCAATTATCAATCCTCCACAGGCCTGCATCCTGGCTGTAGGTAGAGGCAACAAAGTGGTCAAATGGGAAGAAGACTCAAGTGGGCAAGGAAAGGCACACTCTGTGACACAGATGAACTTATCATTATCAGCTGATCATCGAGTCTTTGATTATGACATTGGAGGTAAGCTTTGGATGCCTTGTCCACAAACTTCATGGAAGCCAACAGACTTCTCGTATGAGATACCTTATGAGTTGCAGCCTGTTATCAAGCTACAATTCAGTTATA

Fln Alignment:

Contig10994___MYKPSESQKTEKTFLASTDPVXXXXXXXXXXXXLYEDLQNSQIRKIIAKRLWESKHGTPHLYLSADVMLDPVLNFRKELQEKHGLKISVNDIVIKFAALALKAVPEANAYWSDEKGEVVLCDSIDISIAVATEKGLMTPILRKADQKSLSVISAEVKELANKARVGKLTPSEFQGGTFSISNLGMFPVDRFCAIINPPQACILAVGRGNKVVKWEEDSSGQGKAHSVTQMNLSLSADHRVFDYDIGGK
B8LPX9__________________LHKPSEPSKNEKTLSAPIAPVSLQSPLPLQSSGLYEDLQNSQIRKIIAKRLWESKHGTPHLYLSADVMLDPVLAFRKELQEKHGLKISVNDIVIKVVALALKAVPEANAYWSDEKGEAVLCDSIDVSIAVATEKGLMTPILKNADQKSLSAISTEVKELANKARVGKLSPSEFQGGTFSISNLGMFPVDRFCAIINPPQACILAVGRGNKVVKWEEDSSGQGKACSVTQMNLSLSADHRVFDYDIGGK

SNPs (tot: 16)

Position	A %	C %	G %	T %	Probability
297	33	66			0.997452
346	25			75	0.996473
347	25			75	0.996473
349		75	25		0.996473
350		75		25	0.996473
430	20		80		0.995493
431	20		80		0.995493
502	14			85	0.993533
503	14			85	0.993533
505			14	85	0.993533
506		85		14	0.993533
513	14	85			0.993533
516	85			14	0.993533
577	12			87	0.992554
595	14	85			0.993533
599			85	14	0.993533

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain