SustainPineDB

Assembly Name: biogeco3 v1.0

UniGene Name: biog3_v1.0_unigene10616

Length: 913 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene biog3_v1.0_unigene10616 (antisense)

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene4960	14/15

Source	Descriptions	Term	Type	Identity
AutoFact	3-ketoacyl-CoA synthase n=1 Tax=Picea sitchensis RepID=D5A9Z6_PICSI	-	-	75%
FL-Next	sp=3-ketoacyl-CoA synthase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	67%
Blast2go	3-ketoacyl- synthase	-	-	67%

Source	GOs	Term	Type	Identity
Blast2go	fatty acid biosynthetic process	GO:0006633	Biological Process	67%
Blast2go	response to cold	GO:0009409	Biological Process	67%
Blast2go	very long-chain fatty acid metabolic process	GO:0000038	Biological Process	67%
Blast2go	pollen tube development	GO:0048868	Biological Process	67%
Blast2go	cuticle development	GO:0042335	Biological Process	67%
Blast2go	response to light stimulus	GO:0009416	Biological Process	67%
Blast2go	fatty acid elongase activity	GO:0009922	Molecular Function	67%
Blast2go	membrane	GO:0016020	Cellular Component	67%
Blast2go	endoplasmic reticulum	GO:0005783	Cellular Component	67%

Source	InterPros	Term	Type	Identity
Sma3	ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain	IPR000194	-	-
Sma3	TonB box, conserved site	IPR010916	-	-
Sma3	Chalcone/stilbene synthase, C-terminal	IPR012328	-	-
Sma3	Very-long-chain 3-ketoacyl-CoA synthase	IPR012392	-	-
Sma3	FAE1/Type III polyketide synthase-like protein	IPR013601	-	-
Sma3	3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C-terminal	IPR013747	-	-
Sma3	Thiolase-like, subgroup	IPR016038	-	-
Sma3	Gonadotropin, beta subunit, conserved site	IPR018245	-	-
Sma3	Ribosomal protein S10, conserved site	IPR018268	-	-
Sma3	WD40 repeat, conserved site	IPR019775	-	-

Full-Lengther Next Prediction

Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: D5A9Z6

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 221 aas, atg_distance in limit (1-15): atg_distance = 5, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 274 - 525

Fln protein: MEPSHAECSDKSFDLAENLDFNYIKLGFSDLLSRASMFWFPMLAAGSLVFVIDQLYFWTTSDHMVTTLGLLWLTCGLGISFVYYFRLSRAFYMLEVACYKAPQEWRVPFDHFIEHAVISQKFTAKSVEFQKRILERAAVSEFVAVPPSLHYLPPRLSQKSSRQEAEIVMLGCIQDLLEKTGVAASEIGVLVVNCSVFNPIPSLSSMIVNKFGFRSDIKTYNLGGMGCSANLISVDLATGCLRAARRGTYAIVLSTENITENWYFGNYEPMTVS*

Protein Length: 275

Fln nts: GACAATCTGTGTGTGTAAGGTTTTGAAGGCTGTGTGATCTGAAGAGTAATAATGTGAAT_-_ATGGAACCTAGCCATGCAGAATGCAGCGACAAGTCGTTTGATTTGGCAGAGAATCTGGACTTTAATTACATAAAGCTGGGATTCTCTGATCTGCTCTCTAGGGCATCAATGTTCTGGTTTCCCATGCTGGCAGCTGGTTCGCTCGTATTTGTCATCGACCAGCTCTATTTTTGGACAACCAGTGATCACATGGTCACCACCCTTGGGCTTCTGTGGTTAACTTGTGGTCTGGGGATCAGTTTTGTTTACTATTTTCGTCTGTCAAGGGCATTCTATATGCTGGAGGTGGCCTGTTACAAGGCGCCTCAGGAATGGAGGGTACCGTTTGATCATTTCATTGAACATGCCGTGATTTCTCAGAAATTCACGGCGAAGAGCGTGGAGTTTCAGAAAAGGATTCTGGAAAGAGCTGCTGTGAGCGAATTTGTTGCTGTTCCACCGTCTCTGCATTACTTGCCCCCCAGGTTGTCACAAAAGTCTTCTCGGCAAGAAGCAGAGATCGTGATGTTGGGCTGTATTCAAGATCTGCTAGAGAAAACTGGCGTTGCTGCCAGTGAAATTGGCGTCCTGGTTGTGAATTGCAGTGTGTTTAATCCGATTCCGTCGCTGTCATCCATGATTGTGAATAAATTTGGGTTTCGCAGCGACATCAAGACTTATAACCTCGGCGGGATGGGATGCAGCGCCAACCTAATATCGGTGGATCTGGCCACGGGTTGCCTGCGTGCTGCCAGAAGGGGAACTTACGCAATTGTGCTGAGCACAGAAAACATTACAGAAAATTGGTATTTCGGAAACTATGAGCCCATGACAGTCTCCTAACATCTTGTTCAGAATTGGATGCGGTGCAGTGC

Fln Alignment:

Contig10616___DLAENLDFNYIKLGFSDLLSRASMFWFPMLAAGSLVFVIDQLYFWT-TSDHMVXXXXXXXXXXXXGISFVYYFRLSRAFYMLEVACYKAPQEWRVPFDHFIEHAVISQKFTAKSVEFQKRILERAAVSEFVAVPPSLHYLPPRLSQKSSRQEAEIVMLGCIQDLLEKTGVAASEIGVLVVNCSVFNPIPSLSSMIVNKFGFRSDIKTYNLGGMGCSANLISVDLATGCLRAARRGTYAIVLSTENITENWYFGNYEPMTVS
D5A9Z6__________________DFAETENLNSIKMGYSYLLTSVS--WPRLLATVALVFAIQGIYLWMLVIDEIVWFLPLICAMASILI---YYFCASKQIYMVEVSCYRPPQEWRVSFHNFIEHAMISQKFTAKSVGFQKRILERAAVSEYAAVPPALRYLPPRLTHAASRQEAEIVMFGCVRDLLEKTGVDVHEVGVLVVNCSVFNPIPSLSSLIVNKFGLRSDIKTYNLGGMGCSANLIALDLASACLRVSNRGTYAIVLSTENITENWYFGNYEPMLVS

SNPs (tot: 7)

Position	A %	C %	G %	T %	Probability
343	16		83		0.994512
345	16	83			0.994512
346		83		16	0.994512
347	83			16	0.994512
784	20			80	0.995493
785		20		80	0.995493
788			80	20	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain