SustainPineDB

Assembly Name: biogeco3 v1.0

UniGene Name: biog3_v1.0_unigene10614

Length: 1321 nt

UniGene Fasta

Ace file of the UniGene biog3_v1.0_unigene10614

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene298	23/23

Source	Descriptions	Term	Type	Identity
AutoFact	rhomboid-like protein 15 [Arabidopsis thaliana] sp\|Q8LB17.2\|Y3846_ARATH RecName: Full=Uncharacterized protein At3g58460 gb\|AAM97052.1\| putative protein [Arabidopsis thaliana] gb\|AAN15505.1\| putative protein [Arabidopsis thaliana] gb\|AEE79784.1\| rhomboid-l	-	-	50%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	81%
Blast2go	rhomboid family protein	-	-	72%

Source	ECs	Term	Type	e value	Identity
Blast2go	Hydrolases, Acting on peptide bonds (peptide hydrolases), Serine endopeptidases.	EC:3.4.21.-	-	0.0	72%

Source	GOs	Term	Type	e value	Identity
Blast2go	serine-type endopeptidase activity	GO:0004252	Molecular Function	0.0	72%
Blast2go	integral to membrane	GO:0016021	Cellular Component	0.0	72%

Source	InterPros	Term	Type	Identity
Sma3	Ubiquitin-associated/translation elongation factor EF1B, N-terminal	IPR000449	-	-
Sma3	Peptidase S54, rhomboid	IPR002610	-	-
Sma3	Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote	IPR015940	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: D5A8E7

Fln msg: Overlapping hits, possible frame ERROR between 35 and 29, and overlapping frame ERROR between 97 and 97, your sequence is shorter than subject: 375 - 410

Fln protein: LICLLVGYxxxxEICFWPAQLVLRLQVYRIxxxVVFHGSVLHVMFNMMALVPIGSGLERIMGSVRYLHVTLLLATCNAVLHLLIAYLAAHNPVHPYPFFMDECTIGFSGIIFAMIVMETSLNGNQSRSVFGLFNVPGKWYAWILLILFQLLMPNVSLLGHLCGILSGFAYTYGLFNYFLLGLTSYTAIENSAMLACCVRRPGFIVGGASGAAVNGSLPSFPSTASSGAGLSNFWRNVRSWMPPRESLPNQSLQDPRFPGRGRTLVSSQIQSTSRIGSETELQTRLLDRSSQPQAPSPMMPIITRETVTSNARHTHRENSQQSGPTHMNQGHTQTHDEAIQNLVAMGFEKAKAEVALAAANGDLALAVELLSSQE*

Protein Length: 376

Fln nts: ACTTGATATGCCTGCTGGTTGGATATxxxxxxxxxxxxGAGATCTGTTTCTGGCCAGCACAGTTAGTTTTGCGATTGCAAGTGTATAGGATCTAxxxxxxTGTGGTATTTCATGGTTCGGTGCTGCATGTTATGTTCAATATGATGGCCTTGGTGCCTATTGGCAGTGGGCTAGAGCGAATTATGGGATCGGTTCGATACTTGCATGTCACATTGCTGCTAGCTACCTGCAATGCGGTTTTGCATCTTCTCATTGCTTATCTGGCAGCGCACAACCCAGTGCATCCTTATCCTTTCTTTATGGATGAGTGTACCATTGGCTTCTCTGGTATCATATTTGCTATGATTGTCATGGAAACTAGCTTAAATGGAAATCAGAGTCGAAGTGTATTTGGACTTTTCAATGTCCCTGGAAAATGGTATGCTTGGATCCTTCTTATCCTGTTCCAGCTTCTAATGCCAAATGTTTCACTCTTAGGACATCTCTGTGGCATTTTGTCTGGGTTTGCATATACCTATGGCTTGTTTAACTACTTCTTATTGGGATTAACATCATATACAGCTATCGAGAACTCTGCAATGCTTGCATGCTGTGTAAGGCGCCCTGGATTCATCGTTGGTGGTGCAAGTGGTGCTGCAGTTAATGGATCACTTCCTTCTTTTCCAAGTACAGCGTCTAGTGGTGCAGGTTTAAGTAATTTTTGGAGAAATGTCCGTTCATGGATGCCACCAAGGGAAAGTTTGCCTAATCAGTCTTTGCAAGATCCTAGATTTCCAGGGAGAGGTCGGACGCTTGTGTCTTCCCAAATTCAATCAACATCAAGGATTGGTTCTGAGACAGAACTACAAACTAGACTTCTTGATAGGAGCAGCCAACCACAAGCACCTTCTCCAATGATGCCAATAATCACTCGTGAGACTGTAACATCAAATGCAAGGCACACACATAGGGAAAATTCACAACAATCAGGCCCTACACATATGAATCAGGGACATACACAAACTCATGATGAAGCCATTCAGAATCTTGTTGCAATGGGATTTGAAAAGGCTAAGGCAGAAGTTGCACTAGCTGCAGCAAATGGAGATTTAGCTCTTGCTGTTGAACTTCTGAGCAGTCAGGAATAACAGCATTCTAAATTTTTCTTTAAATTAGCATAGAAGTTCGCAACATGTTTAAGATGAGGATAAAGTTCTCCAATCCTGGAGCGGAAATGTGCATTACACATGGTTCCAATGTAAATAAATGCAGTATTTTTTGTCTTGCTTCTTCCAATTTCCCAGTTTGCTTGCCAATGGATTTTAATTGAGTGCTTTCTACATT

Fln Alignment:

Contig10614___LICLLVGYDxxYEICFWPAQLVLRLQVYRIYTSVVFHGSVLHVMFNMMALVPIGSGLERIMGSVRYLHVTLLLATCNAVLHLLIAYLAAHNPVHPYPFFMDECTIGFSGIIFAMIVMETSLNGNQSRSVFGLFNVPGKWYAWILLILFQLLMPNVSLLGHLCGILSGFAYTYGLFNYFLLGLTSYTAIENSAMLACCVRRPGFIXXXXXXXXXXXXLPSFPSTASSGAGLSNFWRNVRSWMPPRESLPNQSLQDPRFPGRGRTLVSSQIQSTSRIGSETELQTRLLDRXXXXXXXXXXXXIITRETVTSNARHTHRENSQQSGPTHMNQGHTQTHDEAIQNLVAMGFXXXXXXXXXXXXXXXXXXXVELLSSQE
D5A8E7__________________LVCLLVGYDxxYAICFWPAQLILRLQVYRIYTSVVFHGSVLHVMFNMMALVPIGSGLERIMGSVRYLHVTLLLATCNAVLHLLIAYLAAHNPVHPYPFFMDECTIGFSGIIFAMIVMETSLNGNQSRSVFGLFNVPGKWYASILLILFQLLMPNVSLLGHLCGILSGFAYTYGLFNYFLLGLASYTAIESSAMLACCVRRPGFIIGGASGAAATGSLPSFPSTASSGAGLGNVWRSARSLMPQRETLPNQSLQDPRFPGRGRTLGSSQIQSTSRVGSETELQTRLLERSSQPEAPSQMMPISTRETATSNARHTPRENSQESGPTHINQEHTQNH-ETIQNLVAMGFEKAKAEVALDAANGDVALAVELLSSQE

SNPs (tot: 24)

Position	A %	C %	G %	T %	Probability
277	16		83		0.994512
426	33		66		0.997452
430		66		33	0.997452
542	25		75		0.996473
543		25	75		0.996473
585	20	80			0.995493
788	25	75			0.996473
789	25		75		0.996473
790	20		60	20	0.99255
791	80		20		0.995493
792		60		40	1.0
794		80		20	0.995493
802	14			85	0.993533
803	14			85	0.993533
804		85		14	0.993533
805		85	14		0.993533
806		85		14	0.993533
865		14		85	0.993533
867			85	14	0.993533
868			85	14	0.993533
948		60	20		0.99255
1091		25		75	0.996473
1249	66	33			0.997452
1252			66	33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain