SustainPineDB

Assembly Name: biogeco3 v1.0

UniGene Name: biog3_v1.0_unigene8233

Length: 1699 nt

UniGene Fasta

Ace file of the UniGene biog3_v1.0_unigene8233

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene1215	63/64

Source	Descriptions	Term	Type	Identity
AutoFact	non-specific phospholipase C6 [Arabidopsis thaliana] dbj\|BAC22511.1\| phosphatidylglycerol specific phospholipase C [Arabidopsis thaliana] gb\|AEE78437.1\| non-specific phospholipase C6 [Arabidopsis thaliana]	-	-	69%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	68%
Blast2go	protein	-	-	80%

Source	ECs	Term	Type	e value	Identity
Blast2go	Phospholipase C.	EC:3.1.4.3	-	0.0	80%

Source	KEGGs	Term	Identity
Blast2go	Inositol phosphate metabolism	00562	80%
Blast2go	Glycerophospholipid metabolism	00564	80%
Blast2go	Ether lipid metabolism	00565	80%
Blast2go	Metabolic pathways	01100	80%

Source	GOs	Term	Type	e value	Identity
Blast2go	phosphatidylcholine phospholipase C activity	GO:0034480	Molecular Function	0.0	80%
Blast2go	endomembrane system	GO:0012505	Cellular Component	0.0	80%

Source	InterPros	Term	Type	Identity
Sma3	Peptidase M4, C-terminal	IPR001570	-	-
Sma3	Ribosomal protein 60S	IPR001813	-	-
Sma3	Ribosomal protein P2	IPR001859	-	-
Sma3	Ammonium transporter	IPR001905	-	-
Sma3	Villin headpiece	IPR003128	-	-
Sma3	Gelsolin	IPR007122	-	-
Sma3	Gelsolin domain	IPR007123	-	-
Sma3	Phosphoesterase	IPR007312	-	-
Sma3		IPR010256	-	-
Sma3	Ammonium transporter, conserved site	IPR018047	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LR27

Fln msg: Overlapping hits, possible frame ERROR between 674 and 668, your sequence is shorter than subject: 403 - 529

Fln protein: CMNGFVQQALTISKNLSQIVMKGLRPEALPVYKELVKEFGVFDRWFSSLPGPTHPNRLFVYSATSHGATSHIAKKQLAVGYPQKTLFESLHESGLSFGIYYQNIPATLFYRNLRKLKYIPRFHHYGLKFKRHARKGKLPNLAVIEPRYFDLKLFPANDDHPAHDLSNGQRLVKEVYETLRASPQWNETLLIVTYDEHGGFYDHVPTPVRNVPNPDGIIGPNxxxxKFDRLGVRVPTIMVSPWIKKGTVMHRPSGPTPTSEFEHSSIPATVKKMFNLSTNFLTYRDAWAGTFESVLDLKGGPRTDCPVVLPEPSAMRSTPAKEDGKLSEFQKELVQLAAVIKGDHILKSFPDEIGNRMNVKKANDYVEDAMERFVEASMEAIKLGASESTIVEMRPSLTTRNP*

Protein Length: 404

Fln nts: AATGTATGAATGGATTTGTCCAGCAGGCTCTAACTATATCGAAGAATCTGTCACAGATTGTCATGAAAGGATTAAGGCCTGAAGCTCTTCCTGTCTACAAAGAGCTTGTGAAGGAATTTGGGGTATTTGATCGCTGGTTCTCTTCGTTGCCTGGCCCTACTCATCCCAACAGGCTCTTTGTGTACTCTGCAACTTCTCATGGCGCCACGAGCCATATTGCGAAAAAACAGTTAGCAGTTGGGTATCCTCAGAAAACTCTCTTTGAATCACTCCATGAGTCAGGGTTGTCATTTGGGATTTATTACCAGAACATCCCTGCCACTCTGTTTTACAGAAATTTGAGAAAGCTTAAGTACATTCCCAGGTTTCATCACTATGGTCTCAAGTTTAAGCGCCATGCCAGAAAGGGGAAGCTGCCCAATCTAGCGGTTATAGAGCCTCGCTACTTTGATCTCAAGCTCTTTCCTGCCAATGATGACCACCCTGCTCACGATTTATCCAATGGCCAGAGGCTTGTTAAGGAGGTCTATGAGACTCTCAGAGCGAGTCCTCAGTGGAACGAGACGCTTCTGATAGTTACTTATGATGAACATGGTGGGTTTTATGATCATGTGCCTACACCTGTGAGAAATGTCCCAAACCCAGATGGGATTATTGGTCCAAATxxxxxxxxxxxxAAGTTTGATAGATTGGGTGTGAGGGTGCCCACTATCATGGTTTCTCCATGGATCAAGAAAGGCACAGTGATGCACCGTCCCAGTGGGCCAACCCCTACTTCAGAGTTTGAGCATTCATCCATCCCTGCTACTGTCAAGAAGATGTTTAATCTCTCCACCAATTTTCTCACATACAGAGATGCCTGGGCTGGAACTTTTGAAAGTGTCCTGGATCTCAAAGGAGGCCCTCGAACTGATTGCCCTGTTGTTCTGCCCGAACCTAGCGCCATGAGGTCGACCCCTGCAAAGGAAGATGGAAAGCTGAGTGAGTTTCAGAAAGAACTGGTACAGCTCGCTGCAGTCATTAAGGGCGATCACATTCTCAAAAGTTTTCCAGACGAAATTGGGAATCGTATGAATGTGAAAAAAGCAAATGATTATGTTGAAGACGCCATGGAACGGTTTGTGGAAGCAAGTATGGAGGCCATCAAGTTAGGAGCATCAGAGTCCACCATTGTTGAGATGAGGCCTTCTCTTACAACCAGGAACCCTTGAGCCCTGACCATTACCAGACAAAACCCTTTGTTTTATTCCTGCATGTAATGTCAAAGGATGAAGGTAACGTCCTATAGTTAGTCAGGGTCTGTACATGCATGTGGCCCATTTAGTCAGGGTGAAGGCCATTTTTTTATGTTGAAAGTTGAGAGTTGTGTAATGAATAGGTAGATCCAGTATATAAACACTGGTAGATGGCATCATGGCTGTAACATATCACCAGAATACTATACAATTAAGTTGTCGTATTCTACTGATGTGACAGCCCCATCATATTGCATTATCCTTTTTCTGTCAGTTTCTGCAACTAGATGAATTGATTCAATCTTCTGTAAATCCACTACAGGATGTAATGTAGAACTGGAGGCCGTGTTTATCTCTTTACAGCTGCACCCTGCCAGAAGAATATATGACATGAAAAATGTAAAAGTGGCGAGACTTCCAATGTAGAAGTCGAGACCGCTTTTATTTCAAGAAGATTACAACATT

Fln Alignment:

Contig8233___MNGFVQQALTIS-KNLSQIVMKGLRPEALPVYKELVKEFGVFDRWFSSLPGPTHPNRLFVYSATSHGATSHIAKKQLAVGYPQKTLFESLHESGLSFGIYYQNIPATLFYRNLRKLKYIPRFHHYGLKFKRHARKGKLPNLAVIEPRYFDLKLFPANDDHPAHDLSNGQRLVKEVYETLRASPQWNETLLIVTYDEHGGFYDHVPTPVRNVPNPDGIIGPNPxxFKFDRLGVRVPTIMVSPWIKKGTVMHRPSGPTPTSEFEHSSIPATVKKMFNLSTNFLTYRDAWAGTFESVLDLKGGPRTDCPVVLPEPSAMRSTPAKEDGKLSEFQKELVQLAAVIKGDHILKSFPDEIGNRMNVKKANDYVEDAMERFVEASMEAIKLGASESTIVEMRPSLTTRNP*ALTITRQNP
B8LR27_________________MNGFAQQATSLGGSNMSQTVMNGFRPEVVPVYNTLVNEFAVFDRWFASMPASTQPNRLYVHSATSHGATSNIPKL-LADGYPQKTIFESIDESGLSFGIYYEDIPATLFYKNLRKLKYIGKFRSYSTQFKLHAKQGKLPNYVVVEQRYTDTKLLPANDDHPSHDVAEGQKFVKEVYETLRSSPQWNQTLFLITYDEHGGFFDHVPTPVKDVPSPDDIVGPDPxxFKFDRLGVRVPTIMISPWIDKGTVVHGPNGPYPSSQFEHSSIAATVKKLFNLKSDFLTKRDAWAGTFEDVVQKRSSPRTDCPATLPNPPKIRQSGANEEAKLSDFQKELVQLAAILNGDYTLRSYPDEIGKKMNVREGKKYMGDSVRRFFEAGRLAKSLGADDHEIVKMRPSLTTRSTSGSTSNTPRP

SNPs (tot: 48)

Position	A %	C %	G %	T %	Probability
277		33		66	0.997452
403	33	66			1.0
419	20	80			0.995493
423	80			20	0.995493
541		12	87		0.992554
579	14			85	0.993533
580		14		85	0.993533
582		85		14	0.993533
647		33	66		0.997452
650			66	33	0.997452
703	14		85		0.993533
704	14		85		0.993533
706	10		90		0.90298
707	10	70		20	0.999998
708		70		10	0.999998
709		70	10		0.999998
710	90			10	0.90298
718	10		90		0.90298
719		10	90		0.90298
720	10			90	0.90298
746			88	11	0.923564
798	10	90			0.90298
802		90		10	0.90298
851		11		88	0.923564
853		88		11	0.923564
854	88			11	0.923564
869	12		87		0.992554
870		12	87		0.992554
872	87			12	0.992554
873		87		12	0.992554
971	20		80		0.995493
974			80	20	0.995493
982	20		80		0.995493
985		20		80	0.995493
997	80			20	0.995493
1068	75		25		0.996473
1071	25		75		0.996473
1072	20			80	0.995493
1073	80			20	0.995493
1087	75		25		0.996473
1123	14			85	0.993533
1124	14		85		0.993533
1127			85	14	0.993533
1277		20	80		0.995493
1342	25		25	50	0.995533
1349	66			33	0.997452
1350	33			33	0.997753
1374	57		42		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain