SustainPineDB

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Assembly info
Unigenes
SNPs
SSRs
Descriptions
GOs
ECs
KEGGs
InterPros
miRNAs

Assembly Name: biogeco3 v1.0

UniGene Name: biog3_v1.0_unigene6923

Length: 511 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>biog3_v1.0_unigene6923
ACGGCGCACCGAGGGCAGTGACCCTTATTCCGGGGGACGGCATAGGACCCCTCGTAACGGGTGCAGTGGAGCAGGTTATGGAAGCCATGCATGCGCCCGTGTACTTTGAGACGTATGAAGTCAGCGGGAAGATGCAGAGAGTGCCCGCCGAGGTGATCGATTCCATCAAGAAGAATAAGGTGTGCCTGAAGGGTGGTCTGGCCACTCCTGTGGGAGGTGGGGTCAGTTCTTTGAACGTTCAGTTGAGAAAGGAGCTGGACCTGTTTGCGTCTCTTGTTCATTGCTTTAATTTACCCGGCCTCCCCACTCGCCACGACAATGTTGATATTGTTGTTATCAGGGAGAATACGGAGGGGGAATACGCTGGTCTAGAGCACGAGGTCGTACCTGGTGTTGTCGAAAGCCTCAAGGTAAATGTTCATCATAAATTGTTCTCTCAATTTCTTGATTATAATTGTTATATCCTGGTCCTAAGTCACTAGCAAGGTCAAAGACTCAAGCTTGACGCACT

Ace file of the UniGene biog3_v1.0_unigene6923 (antisense)

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Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene10017	7/7

Source	Descriptions	Term	Type	Identity
AutoFact	Isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial n=4 Tax=Brassicaceae RepID=IDH1_ARATH	-	-	83%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	97%
Blast2go	isocitrate dehydrogenase	-	-	94%

Source	ECs	Term	Type	e value	Identity
Blast2go	Isocitrate dehydrogenase (NAD(+)).	EC:1.1.1.41	-	0.0	94%

Source	KEGGs	Term	Identity
Blast2go	Citrate cycle (TCA cycle)	00020	94%
Blast2go	Biosynthesis of terpenoids and steroids	01062	94%
Blast2go	Biosynthesis of alkaloids derived from shikimate pathway	01063	94%
Blast2go	Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid	01064	94%
Blast2go	Biosynthesis of alkaloids derived from histidine and purine	01065	94%
Blast2go	Biosynthesis of alkaloids derived from terpenoid and polyketide	01066	94%
Blast2go	Biosynthesis of plant hormones	01070	94%
Blast2go	Metabolic pathways	01100	94%
Blast2go	Biosynthesis of secondary metabolites	01110	94%

Source	GOs	Term	Type	Identity
Blast2go	tricarboxylic acid cycle	GO:0006099	Biological Process	94%
Blast2go	isocitrate metabolic process	GO:0006102	Biological Process	94%
Blast2go	NAD binding	GO:0051287	Molecular Function	94%
Blast2go	magnesium ion binding	GO:0000287	Molecular Function	94%
Blast2go	isocitrate dehydrogenase (NAD+) activity	GO:0004449	Molecular Function	94%
Blast2go	zinc ion binding	GO:0008270	Molecular Function	94%
Blast2go	mitochondrion	GO:0005739	Cellular Component	94%

Source	InterPros	Term	Type	e value	Identity
Sma3	Isocitrate dehydrogenase NAD-dependent	IPR004434	-	0.0	-
Sma3	Isocitrate/isopropylmalate dehydrogenase	IPR001804	-	0.0	-

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: B8LPK1

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 163 aas, your sequence is shorter than subject: 160 - 378

Fln protein: GAPRAVTLIPGDGIGPLVTGAVEQVMEAMHAPVYFETYEVSGKMQRVPAEVIDSIKKNKVCLKGGLATPVGGGVSSLNVQLRKELDLFASLVHCFNLPGLPTRHDNVDIVVIRENTEGEYAGLEHEVVPGVVESLKVNVHHKLFSQFLDYNCYILVLSH*

Protein Length: 161

Fln nts: ACGGCGCACCGAGGGCAGTGACCCTTATTCCGGGGGACGGCATAGGACCCCTCGTAACGGGTGCAGTGGAGCAGGTTATGGAAGCCATGCATGCGCCCGTGTACTTTGAGACGTATGAAGTCAGCGGGAAGATGCAGAGAGTGCCCGCCGAGGTGATCGATTCCATCAAGAAGAATAAGGTGTGCCTGAAGGGTGGTCTGGCCACTCCTGTGGGAGGTGGGGTCAGTTCTTTGAACGTTCAGTTGAGAAAGGAGCTGGACCTGTTTGCGTCTCTTGTTCATTGCTTTAATTTACCCGGCCTCCCCACTCGCCACGACAATGTTGATATTGTTGTTATCAGGGAGAATACGGAGGGGGAATACGCTGGTCTAGAGCACGAGGTCGTACCTGGTGTTGTCGAAAGCCTCAAGGTAAATGTTCATCATAAATTGTTCTCTCAATTTCTTGATTATAATTGTTATATCCTGGTCCTAAGTCACTAGCAAGGTCAAAGACTCAAGCTTGACGCACT

Fln Alignment:

Contig6923___GAPRAVTLIPGDGIGPLVTGAVEQVMEAMHAPVYFETYEVSGKMQRVPAEVIDSIKKNKVCLKGGLATPVGGGVSSLNVQLRKELDLFASLVHCFNLPGLPTRHDNVDIVVIRENTEGEYAGLEHEVVPGVVESLKV
B8LPK1_________________GSPRAVTLIPGDGIGPLVTGAVEQVMEAMHAPVYFETYEVSGKMDKVPAEVIDSIKKNKVCLKGGLATPVGGGVSSLNVQLRKELDLFASLVHCFNLPGLPTRHDNVDIVVIRENTEGEYAGLEHEVVPGVVESLKV

SNPs (tot: 14)

Position	A %	C %	G %	T %	Probability
245	25		75		0.996473
246		75		25	0.996473
247	75			25	0.996473
294	33	66			1.0
296		66		33	1.0
297		66	33		1.0
299	16	83			0.994512
301	16	83			0.994512
302	83			16	0.994512
303		83		16	0.994512
365	20		80		0.995493
367	20		80		0.995493
368			80	20	0.995493
369			80	20	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain