SustainPineDB

Assembly Name: biogeco3 v1.0

UniGene Name: biog3_v1.0_unigene6057

Length: 1045 nt

UniGene Fasta

Ace file of the UniGene biog3_v1.0_unigene6057

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene3475	32/32

Source	Descriptions	Term	Type	Identity
AutoFact	Potassium transporter HAK2p n=1 Tax=Mesembryanthemum crystallinum RepID=Q94KC6_MESCR	-	-	66%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	91%
Blast2go	potassium transporter 2	-	-	81%

Source	GOs	Term	Type	Identity
Blast2go	potassium ion transmembrane transporter activity	GO:0015079	Molecular Function	81%
Blast2go	plasma membrane	GO:0005886	Cellular Component	81%
Blast2go	potassium ion transmembrane transport	GO:0071805	Biological Process	81%

Source	InterPros	Term	Type	Identity
Sma3	Zinc finger, NF-X1-type	IPR000967	-	-
Sma3	Single-stranded nucleic acid binding R3H	IPR001374	-	-
Sma3	Peptidase aspartic, active site	IPR001969	-	-
Sma3	Multicopper oxidase, copper-binding site	IPR002355	-	-
Sma3	Immunoglobulin/major histocompatibility complex, conserved site	IPR003006	-	-
Sma3	K+ potassium transporter	IPR003855	-	-
Sma3	Zinc finger, C2H2	IPR007087	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-
Sma3		IPR018519	-	-

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: coniferopsida.fasta

Fln subject: D5A8F8

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 33 aas, your sequence is longer than subject: 330 - 250

Fln protein: MGNASGLAVITVMLVTTCLTSLVIIICWHKSILLAVCFLLFFGSIEALYFSASLIKFLEGAWVPILLALIFMTIMYVWHYGTIKKYDFDLQNKVSIKWLLSLGPSLGIVRVPGIGLMYTELVTGIPANFTHFVTNLPAFHQVLVFICIKSVPVPYVPAEERFLIGRVGPKEYRLYRCIVRYGYRDVHGDTDDFEDQLILNLGEFIRSEAKHSCSSDNYSADSADGRMAVIGTPVQGLESILSYETTEESIQSVNLSHLPSQTIQSIQDLMETDSPHLARRRKVRFLLPSSPDIDSDVKDELQELFQARESGVAFILGHSYVKAKNDSSF*

Protein Length: 331

Fln nts: AATGTGCTGTTACTATTGGTTTTCAAGATACCAAACGA_-_ATGGGAAATGCATCTGGTTTAGCTGTCATCACAGTCATGCTAGTTACTACCTGCCTTACATCATTGGTAATAATTATATGCTGGCATAAGAGTATTCTACTAGCAGTATGCTTTCTACTTTTCTTTGGATCTATCGAAGCCCTCTACTTCTCAGCTTCTCTCATTAAGTTTCTAGAAGGGGCCTGGGTACCTATTCTTCTGGCTCTCATATTCATGACTATCATGTATGTGTGGCATTATGGGACAATAAAGAAGTATGACTTCGATTTGCAAAACAAGGTATCCATAAAATGGCTGCTTTCACTTGGTCCTAGTTTGGGGATTGTGCGTGTCCCTGGAATTGGCCTTATGTACACAGAACTTGTCACTGGGATACCTGCAAATTTTACACATTTTGTAACAAATCTTCCAGCATTCCATCAGGTGCTTGTCTTTATATGCATTAAATCGGTGCCAGTGCCGTATGTTCCCGCAGAAGAACGGTTTCTCATTGGTCGCGTAGGGCCTAAAGAGTACAGGTTATACAGATGCATTGTTCGATATGGTTACCGCGATGTCCATGGGGATACTGATGATTTTGAGGATCAGCTTATTCTCAACCTCGGAGAGTTTATTCGTTCAGAAGCAAAACATTCTTGCTCAAGTGACAATTACTCTGCAGACTCTGCAGATGGAAGAATGGCTGTGATTGGAACCCCTGTGCAAGGCTTGGAGAGCATTCTAAGTTACGAAACAACAGAAGAGAGCATACAGTCTGTTAATCTATCCCATCTTCCGTCACAGACCATCCAAAGCATACAGGATCTTATGGAAACTGACTCTCCTCATCTGGCTCGGAGGAGGAAAGTCCGATTTTTGCTGCCTAGCAGTCCTGATATTGATTCTGATGTAAAGGATGAGTTGCAAGAACTCTTTCAGGCTAGGGAATCTGGAGTTGCCTTTATATTAGGGCACTCTTATGTTAAAGCAAAAAATGACTCGTCTTTTTAAAAAGATTGCCATACATT

Fln Alignment:

Contig6057___MYTELVTGIPANFTHFVTNLPAFHQVLVFICIKSVPVPYVPAEERFLIGRVGPKEYRLYRCIVRYGYRDVHGDTDDFEDQLILNLGEFIRSEAKHSCSSDNYSADSADGRMAVIGTPVQGLESILSYETTEESIQSVNLSHLPSQTIQSIQDLMETDSPHLARRRKVRFLLPSSPDIDSDVKDELQELFQARESGVAFILGHSYVKAKNDSSF*K
D5A8F8_________________MYTELVTGIPANFTHFVTNLPAFHQVLIFICIKSVPVPYVPPEERFLIGRVGPKEYRLYRCIVRYGYRDVHGDTDHFEDQLILNLGEFIRSEAKHSCSSDSY---SADGRMAVIGTPVHGMESILSYETAEESIQSVNLSYLGSQTIQSIQDLIETDSPHLARRKKVRFLLPSSPDIDPDVKDELQELFQAREAGIAFILGHSYVKAKNDSSFLK

SNPs (tot: 32)

Position	A %	C %	G %	T %	Probability
100	33	66			0.997452
119	25		75		0.996473
120	25	75			0.996473
123			75	25	0.996473
146	20			80	0.995493
241	16		83		0.994512
242	16	83			0.994512
244		83	16		0.994512
252	14	85			0.993533
333	25		75		0.996473
334	25	75			0.996473
337		75		25	0.996473
445		83		16	0.994512
635	11			88	0.923564
638	85			14	0.993533
639		85		14	0.993533
683	25		75		1.0
687		75		25	1.0
734		87		12	0.992554
735		87	12		0.992554
736		87		12	0.992554
771	75			25	1.0
772	75			25	1.0
783		14	85		0.993533
785			85	14	0.993533
786		85		14	0.993533
792		16	83		0.994512
793	16			83	0.994512
794		83		16	0.994512
843		11		88	0.923564
844	11	88			0.923564
1007		90		10	0.90298

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain