SustainPineDB

Assembly Name: biogeco3 v1.0

UniGene Name: biog3_v1.0_unigene5866

Length: 1185 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene biog3_v1.0_unigene5866 (antisense)

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene2256	27/27

Source	Descriptions	Term	Type	Identity
AutoFact	Calcium-dependent protein kinase, putative n=1 Tax=Ricinus communis RepID=B9RPG6_RICCO	-	-	76%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	96%
Blast2go	cdpk-related protein kinase	-	-	89%

Source	ECs	Term	Type	e value	Identity
Blast2go	Calcium/calmodulin-dependent protein kinase.	EC:2.7.11.17	-	0.0	89%

Source	GOs	Term	Type	Identity
Blast2go	protein phosphorylation	GO:0006468	Biological Process	89%
Blast2go	calmodulin-dependent protein kinase activity	GO:0004683	Molecular Function	89%
Blast2go	calcium ion binding	GO:0005509	Molecular Function	89%
Blast2go	ATP binding	GO:0005524	Molecular Function	89%

Source	InterPros	Term	Type	Identity
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Calcium-binding EF-hand	IPR002048	-	-
Sma3	Serine/threonine- / dual-specificity protein kinase, catalytic domain	IPR002290	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	EF-hand-like domain	IPR011992	-	-
Sma3	Calmodulin-binding domain, plant	IPR012417	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-
Sma3	EF-hand	IPR018248	-	-
Sma3	EF-HAND 2	IPR018249	-	-
Sma3	ATPase, P-type phosphorylation site	IPR018303	-	-

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NWM8

Fln msg: STOP codon was not found. Distance to subject end: 15 aas, your sequence is shorter than subject: 394 - 589

Fln protein: MSIAIEDVRREVKILKALSGHHNLVKFYDSCEDANNVYIIMELCEGGELLDRILSRGGKYPEEDAKAIVRQILSIVAFCHIQGVVHRDLKPENFLFTTKDEHAPMKAIDFGLSDFIKPDERLNDIVGSAYYVAPEVLHRSYSMEADVWSVGVITYILLCGSRPFWARTESGIFRAVLRADPNFDDAPWPSVSPEAKDFVKRLLNKDLRKRMTAAQALTHPWLRGQHAIPLDILIFRLVKAYIRASPLKRAALKALSKSLTENELFYLRSQFKLLEPNKDGKVSLENFRLALTRNATDAMKESKVPDILSSMEHLASRRMDFEEFCAAAISVYQLEALDRWEQIASTAYEFFEQEGNKVISVEELAREMNLAPTAHTVLHDWIRSTDGKLSFHGT

Protein Length: 395

Fln nts: AATGTCTATAGCAATTGAAGATGTGCGGAGAGAGGTCAAAATTTTAAAAGCATTGTCGGGCCATCACAACTTGGTCAAATTCTATGATTCTTGCGAGGATGCTAACAATGTCTACATAATAATGGAACTATGTGAAGGTGGTGAGTTACTGGACAGAATTTTGTCACGAGGTGGCAAGTATCCAGAAGAAGATGCAAAAGCTATTGTGAGACAAATCTTGAGCATAGTGGCTTTTTGTCACATTCAAGGTGTTGTGCACCGTGATTTGAAGCCAGAGAATTTTCTATTCACCACTAAGGATGAACATGCACCTATGAAGGCTATTGATTTTGGGCTCTCGGATTTCATCAAACCAGATGAGAGACTGAATGATATAGTAGGCAGCGCATATTATGTTGCGCCAGAAGTTCTACATCGATCATATAGTATGGAAGCTGATGTATGGAGTGTTGGTGTCATAACATACATTTTATTATGTGGAAGTCGACCTTTTTGGGCAAGGACCGAATCAGGAATTTTCCGTGCTGTTTTAAGAGCAGATCCTAATTTTGATGATGCACCATGGCCATCAGTATCTCCTGAAGCAAAGGATTTTGTGAAAAGGCTTCTTAACAAAGACTTGCGCAAAAGAATGACAGCTGCACAGGCCCTTACACATCCATGGCTTCGAGGTCAACATGCAATCCCGTTGGATATTTTGATTTTCAGATTAGTCAAAGCATATATTCGGGCTTCTCCTTTGAAACGGGCAGCTCTGAAGGCACTGTCAAAATCATTGACAGAAAATGAATTATTTTACCTCAGATCACAGTTTAAACTTCTTGAACCCAACAAAGATGGAAAGGTTTCCCTGGAAAACTTTAGATTGGCTTTAACGAGGAATGCGACTGACGCGATGAAGGAATCTAAGGTTCCTGATATTCTCAGTTCGATGGAACATCTTGCTTCAAGAAGAATGGACTTTGAAGAGTTCTGTGCTGCTGCAATAAGTGTATATCAGCTGGAAGCCCTTGACCGGTGGGAGCAGATTGCAAGCACTGCATATGAATTCTTTGAACAGGAGGGCAATAAGGTTATTTCAGTTGAGGAATTAGCTCGGGAAATGAACCTTGCACCTACTGCACATACGGTTCTACATGATTGGATCAGAAGCACTGACGGGAAACTAAGTTTTCACGGGACATT

Fln Alignment:

Contig5866___SIAIEDVRREVKILKALSGHHNLVKFYDSCEDANNVYIIMELCEGGELLDRILSRGGKYPEEDAKAIVRQILSIVAFCHIQGVVHRDLKPENFLFTTKDEHAPMKAIDFGLSDFIKPDERLNDIVGSAYYVAPEVLHRSYSMEADVWSVGVITYILLCGSRPFWARTESGIFRAVLRADPNFDDAPWPSVSPEAKDFVKRLLNKDLRKRMTAAQALTHPWLRGQHAIPLDILIFRLVKAYIRASPLKRAALKALSKSLTENELFYLRSQFKLLEPNKDGKVSLENFRLALTRNATDAMKESKVPDILSSMEHLASRRMDFEEFCAAAISVYQLEALDRWEQIASTAYEFFEQEGNKVISVEELAREMNLAPTAHTVLHDWIRSTDGKLSFHG
A9NWM8_________________AIAIEDVRREVKILKALSGHHNLVKFHDACEDANNVYIIMELCEGGELLDRILSRGGKYPEEDAKVIVRQILSIVAFCHIQGVVHRDLKPENFLFTTKDEHAPMKAIDFGLSDFIRPDERLNDIVGSAYYVAPEVLHRSYSMEADVWSVGVITYILLCGSRPFWARTESGIFRAVLRADPNFDDAPWPSVSPEAKDFVKRLLNKDLRKRMTAAQALTHPWLRGQHAIPLDILIFRLVKAYIRASPLKRAALKALSKSLTENELFYLRSQFKLLEPNKDGKISLENFRLALMRNATEAMKESKVPDILNTMEHLASRRMDFEEFCAAAISIYQLEAVDRWEQIASTAYEFFEQEGNKVISVEELAREMNLAPTAHTVLHDWIRSTDGKLSFHG

SNPs (tot: 22)

Position	A %	C %	G %	T %	Probability
330	33			66	0.997452
331	33			66	0.997452
332		66		33	0.997452
333		66	33		0.997452
334	66			33	0.997452
548		83		16	0.994512
557		20		80	0.995493
558	20			80	0.995493
669	66	33			0.997452
792		66	33		0.997452
793	66			33	0.997452
809	25			75	0.996473
811		75	25		0.996473
889	20	80			0.995493
890	20	80			0.995493
948	20		20	60	0.99255
949		25	75		0.996473
951		75		25	0.996473
952	75			25	0.996473
1070		14		85	0.993533
1071	85	14			0.993533
1072		14		85	0.993533

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain