SustainPineDB

Assembly Name: biogeco3 v1.0

UniGene Name: biog3_v1.0_unigene4485

Length: 2037 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>biog3_v1.0_unigene4485
TATTTAAGGGTAAGGGTCAACCTGGACTCCATTGACACAGTCAGTCACTCTTATTTCGGGCCATTAATGAAAACCTTGCCACTATAATTCCTCCCCGGGCATAGGCAACACCCGCGCCGGTTCATATAAACCCTCCGCTGTAACGCCATTCGCGTGAATACTTTTTTAGATCACAGAACAAATCTTACAATTTTTGTCGATCAATTTCCTTAGAGGGCCATGGATGAGGAATACGATGTGATAGTGCTGGGCACTGGTCTGAAAGAATGCATCCTCAGCGGTCTCCTTTCTGTGGATCGGCTCAAGGTCCTCCACATGGACAGAAATGATTACTATGGAGGAGATTCTACTTCTCTTAATCTCATCCAGCTTTGGAAGAAGTTCCGAGGTAACGATAAGCCTCCTGCTCATCTCGGATCCAGTAGAGACTACAATGTTGATATGATCCCTAAGTTTATGATGGCAAATGGTAGTTTGGTGCGAGTCCTCATTCACACAGATGTTACAAAATATTTGTATTTTAAGGCTGTGGACGGTAGTTATGTATACAATAAAGGAAAGATATACAAAGTCCCAGCGACTGATGTGGAAGCTCTAAAATCTCCTCTTATGGGAATATTTGAAAAGCGTCGTGCTAGAAAGTTCTTTATTTATGTTCAAAATTATGACGAGAATGATCCAACAACACATGATGGCATGGACTTGACAAGAGTAACAGCAAGAGAACTTATTGCGAAATATGGATTAGATGATAATACTGTAGATTTCATTGGCCATGCATTGGCACTTCATCGGGATGACCGTTATCTATATGAGCCTGCATTAGAGACTGTAAAAAAATAAAGCTATACGAAGAATCTCTTGCTCGTTTTCAAGGAGGGTCGCCATACATATACCCATTGTATGGGTTGGGTGAACTACCTCAGGGATTTGCACGTCTTAGTGCTGTCTATGGAGGAACATACATGCTGAACAAACCGGAGTGCAAGGTTGAGTTTGATGAAGAAGGTAAGGTGTGTGGTGTGACATCTGAAGGGGAGACAGCCAGATGCAAAAAAGTAGTTTGTGATCCATCATATTTACCTAACAAGGTCAAGAAAATTGGAAAGGTTGCTCGTGCAATTTGTGTAATGAGCCACCCTATCCCAAACACAAATGACTCCCAATCAGTACAGATAATTCTGCCTCAGAAGCAACTTGGACGCAAGTCTGACATGTATGTATTTTGCTGTTCTTATTCACATAATGTTGCTCCCAAAGGGAAGTACATTGCTTTTGTTTCGGCTGAGGCAGAAACAGATCAACCAGAGGTAGAATTGCAACCGGGAATAGCTCTTCTTGGACCTGTTGATGAGATGTTCTTTGATACATATGATAGATTTGCTCCTGTTAATGAGCCTTCTCTTGACAATTGCTTCATATCCACTAGCTATGATGCGACAACACATTTTGAGACAACTGTGCAGGATGTTCTTGCCATGTACACCAACATAACTGGAAAGGTTTTGGACCTAAGTGTTGACCTAAGTGCTGCAAGTGCGGCTGAAGAATAATGGGCTGTAGCATATCAACCTTTAGTTGGGTTACACATTCCTCTAAGGCTGTACTTGTTTTGATATGATCTACAGTGTGTTGAAGTTTTAGTGTTTGTATGTATTTGGGAATCACGCCTTTACCTGGTGTTTTAATATCTGGAATGTTCAGGTCCAGACATAGAACACTGTTTTTTCAGCTGTTCATTCAAATCACTGGCTTTTATATCATTGATATGTATGAATTTGTCTGCAATAATTGTTCAGTGTTCAGTTTGATAGGAAGACAAGACGTAGAGGTTCTGACTGTGGGTGTTGGTAATCATGTAATATTTTCATCCATGCTTGAAACTCTGCTATGATTTATGGTGCGGGATATTAGTATTTAATCATAAAAATCACTAATATTCAGATGGTAATTTGTATTTGAAGTGAACAAGGTGCTTCACCTACGAGAACATCATGGATACTTAAAAGTAATCCCATATATCCATATTAAGACATT

Ace file of the UniGene biog3_v1.0_unigene4485 (antisense)

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene16032	218/234
SustainPine v2.0	sp_v2.0_unigene61647	13/234

Source	Descriptions	Term	Type	Identity
AutoFact	GDP dissociation inhibitor protein OsGDI1 [Oryza sativa]	-	-	85%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	91%
Blast2go	protein	-	-	89%

Source	GOs	Term	Type	e value	Identity
Blast2go	protein binding	GO:0005515	Molecular Function	0.0	89%

Source	InterPros	Term	Type	Identity
Sma3	Rab GDI protein	IPR000806	-	-
Sma3	Myosin head, motor domain	IPR001609	-	-
Sma3		IPR002005	-	-
Sma3	GDP dissociation inhibitor	IPR018203	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NWR2

Fln msg: Overlapping hits, possible frame ERROR between 882 and 834,

Fln protein: MDEEYDVIVLGTGLKECILSGLLSVDRLKVLHMDRNDYYGGDSTSLNLIQLWKKFRGNDKPPAHLGSSRDYNVDMIPKFMMANGSLVRVLIHTDVTKYLYFKAVDGSYVYNKGKIYKVPATDVEALKSPLMGIFEKRRARKFFIYVQNYDENDPTTHDGMDLTRVTARELIAKYGLDDNTVDFIGHALALHRDDRYLYEPALETxxxxxxxxxxxxxxxxxxPYIYPLYGLGELPQGFARLSAVYGGTYMLNKPECKVEFDEEGKVCGVTSEGETARCKKVVCDPSYLPNKVKKIGKVARAICVMSHPIPNTNDSQSVQIILPQKQLGRKSDMYVFCCSYSHNVAPKGKYIAFVSAEAETDQPEVELQPGIALLGPVDEMFFDTYDRFAPVNEPSLDNCFISTSYDATTHFETTVQDVLAMYTNITGKVLDLSVDLSAASAAEE*

Protein Length: 446

Fln nts: TATTTAAGGGTAAGGGTCAACCTGGACTCCATTGACACAGTCAGTCACTCTTATTTCGGGCCATTAATGAAAACCTTGCCACTATAATTCCTCCCCGGGCATAGGCAACACCCGCGCCGGTTCATATAAACCCTCCGCTGTAACGCCATTCGCGTGAATACTTTTTTAGATCACAGAACAAATCTTACAATTTTTGTCGATCAATTTCCTTAGAGGGCC_-_ATGGATGAGGAATACGATGTGATAGTGCTGGGCACTGGTCTGAAAGAATGCATCCTCAGCGGTCTCCTTTCTGTGGATCGGCTCAAGGTCCTCCACATGGACAGAAATGATTACTATGGAGGAGATTCTACTTCTCTTAATCTCATCCAGCTTTGGAAGAAGTTCCGAGGTAACGATAAGCCTCCTGCTCATCTCGGATCCAGTAGAGACTACAATGTTGATATGATCCCTAAGTTTATGATGGCAAATGGTAGTTTGGTGCGAGTCCTCATTCACACAGATGTTACAAAATATTTGTATTTTAAGGCTGTGGACGGTAGTTATGTATACAATAAAGGAAAGATATACAAAGTCCCAGCGACTGATGTGGAAGCTCTAAAATCTCCTCTTATGGGAATATTTGAAAAGCGTCGTGCTAGAAAGTTCTTTATTTATGTTCAAAATTATGACGAGAATGATCCAACAACACATGATGGCATGGACTTGACAAGAGTAACAGCAAGAGAACTTATTGCGAAATATGGATTAGATGATAATACTGTAGATTTCATTGGCCATGCATTGGCACTTCATCGGGATGACCGTTATCTATATGAGCCTGCATTAGAGACTxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxCCATACATATACCCATTGTATGGGTTGGGTGAACTACCTCAGGGATTTGCACGTCTTAGTGCTGTCTATGGAGGAACATACATGCTGAACAAACCGGAGTGCAAGGTTGAGTTTGATGAAGAAGGTAAGGTGTGTGGTGTGACATCTGAAGGGGAGACAGCCAGATGCAAAAAAGTAGTTTGTGATCCATCATATTTACCTAACAAGGTCAAGAAAATTGGAAAGGTTGCTCGTGCAATTTGTGTAATGAGCCACCCTATCCCAAACACAAATGACTCCCAATCAGTACAGATAATTCTGCCTCAGAAGCAACTTGGACGCAAGTCTGACATGTATGTATTTTGCTGTTCTTATTCACATAATGTTGCTCCCAAAGGGAAGTACATTGCTTTTGTTTCGGCTGAGGCAGAAACAGATCAACCAGAGGTAGAATTGCAACCGGGAATAGCTCTTCTTGGACCTGTTGATGAGATGTTCTTTGATACATATGATAGATTTGCTCCTGTTAATGAGCCTTCTCTTGACAATTGCTTCATATCCACTAGCTATGATGCGACAACACATTTTGAGACAACTGTGCAGGATGTTCTTGCCATGTACACCAACATAACTGGAAAGGTTTTGGACCTAAGTGTTGACCTAAGTGCTGCAAGTGCGGCTGAAGAATAA___TGGGCTGTAGCATATCAACCTTTAGTTGGGTTACACATTCCTCTAAGGCTGTACTTGTTTTGATATGATCTACAGTGTGTTGAAGTTTTAGTGTTTGTATGTATTTGGGAATCACGCCTTTACCTGGTGTTTTAATATCTGGAATGTTCAGGTCCAGACATAGAACACTGTTTTTTCAGCTGTTCATTCAAATCACTGGCTTTTATATCATTGATATGTATGAATTTGTCTGCAATAATTGTTCAGTGTTCAGTTTGATAGGAAGACAAGACGTAGAGGTTCTGACTGTGGGTGTTGGTAATCATGTAATATTTTCATCCATGCTTGAAACTCTGCTATGATTTATGGTGCGGGATATTAGTATTTAATCATAAAAATCACTAATATTCAGATGGTAATTTGTATTTGAAGTGAACAAGGTGCTTCACCTACGAGAACATCATGGATACTTAAAAGTAATCCCATATATCCATATTAAGACATT

Fln Alignment:

Contig4485___MDEEYDVIVLGTGLKECILSGLLSVDRLKVLHMDRNDYYGGDSTSLNLIQLWKKFRGNDKPPAHLGSSRDYNVDMIPKFMMANGSLVRVLIHTDVTKYLYFKAVDGSYVYNKGKIYKVPATDVEALKSPLMGIFEKRRARKFFIYVQNYDENDPTTHDGMDLTRVTARELIAKYGLDDNTVDFIGHALALHRDDRYLYEPALETVxxxxxxxxxxxxxxxxSPYIYPLYGLGELPQGFARLSAVYGGTYMLNKPECKVEFDEEGKVCGVTSEGETARCKKVVCDPSYLPNKVKKIGKVARAICVMSHPIPNTNDSQSVQIILPQKQLGRKSDMYVFCCSYSHNVAPKGKYIAFVSAEAETDQPEVELQPGIALLGPVDEMFFDTYDRFAPVNEPSLDNCFISTSYDATTHFETTVQDVLAMYTNITGK
A9NWR2_________________MDEEYDVIVLGTGLKECILSGLLSVDRLKVLHMDRNDYYGGESTSLNLTQLWKKFRGNDKPPAHLGPSRDYNVDMIPKFMMANGSLVRVLIHTDVTKYLYFKAVDGSYVYNKGKIYKVPATDVEALKSPLMGIFEKRRARKFFIYVQNYDQNDPKTHDGMDLTRVTTRELIAKYGLDDNTVDFIGHALALHRDDRYLFEPALETVxxxxxxxxxxxxxxxxSPYIYPLYGLGELPQGFARLSAVYGGTYMLNKPECKVEFDEEGKVCGVTSEGETARCKKVVCDPSYLTNKVRKVGKVARAICIMSHPIPNTNDSQSVQIILPQKQLGRKSDMYVFCCSYSHNVAPKGKYIAFVSAEADTDQPEVELQPGIALLGPVDEMFFDTYDRFVPVNEPSLDNCFISTSYDATTHFETTVQDVLAMYTHITGK

SNPs (tot: 41)

Position	A %	C %	G %	T %	Probability
102			33	66	0.997452
216	16	83			0.994512
217	16			83	0.994512
279		25		75	0.996473
282	75			25	0.996473
288	25	75			0.996473
330	33			66	1.0
331	33		66		1.0
332			66	33	1.0
333	66		33		1.0
334		66		33	1.0
342	10			90	0.90298
344		90	10		0.90298
345		90		10	0.90298
708		7		92	0.999532
742		8	91		0.999766
743	8			91	0.999766
794	8			92	0.999718
1461	10		90		0.999898
1462		90		10	0.999898
1646	13		86		0.999973
1710	11			88	0.923564
1732		85	14		0.993533
1733		85		14	0.993533
1737	85		14		0.993533
1738			83	16	0.994512
1739	16	83			0.994512
1740		83		16	0.994512
1743	16			83	0.994512
1816	25	75			0.996473
1820			75	25	0.996473
1932			50	50	1.0
1933			50	50	1.0
1956		16		83	0.994512
1958			83	16	0.994512
2012	66	33			0.997452
2013		33	66		0.997452
2014		33		66	0.997452
2016		66	33		0.997452
2018		33	66		0.997452
2019	33		66		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain