SustainPineDB

Assembly Name: biogeco3 v1.0

UniGene Name: biog3_v1.0_unigene3890

Length: 2120 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>biog3_v1.0_unigene3890
TTGTTGCCAGTAAAGGTCAGTCAGGAGGGAAAAATTCGTGCAAAAAGGGGTCTTCGACATCTGGCCGACAAAACTACGGCTCCAATTGTTGAGAACACATTACAAGATTGGAGTGAATCTGCAGTGGAACTGAAGAACCCATCACAAACATCTGTCAAGAATTTTCCAAGTTCATTTACATCACGTTATGCAGATAATGAGCCAAGAAAGGGTGCAGATGTCATTGTAGAGTCTTTAGAGCGAGAGGGTGTTGAGCATGTCTTCGCTTACCCTGGAGGTGCATCTATGGAAATTCATCAGGCACTTACTCGATCCAAGACCATAAAAAATGTCCTTTGTCGCCATGAACAAGGAGAGATATTTGCAGCAGAGGGTTATGCCAGGTCTACAGGAAAGGTCGCTGTTTGCATTGCAACTTCTGGTCCTGGTGCCACCAACTTGGTAAGTGGTCTTGCTGATGCATTGCTTGACAGCATTCCTTTGGTTGCTATTACTGGTCAGGTGCCTCGGAGAATGATAGGCACAGATGCCTTTCAAGAAACTCCGATTGTGGAGGTGACTAGGTCTATAACAAAGCACAATTACTTGATCATGGATGTGGATGATATACCCCGAGTCATCAGAGAGGCCTTTTATCTTGCAGCATCTGGGAGGCCCGGACCTGTTTTAGTTGATATCCCAAAGGATGTTCAGCAGCAGATGACTGTTCCAGATTGGAACAAGGCAATAAAACTTCATGGTTATACTGAAAGACTCCCAAAGGCCCCACAGATTTCTCAGTTAGAACAGATCCTGCGCCTTGTGTTTAACTCTAATAAACCTGTCCTTTACGTTGGTGGGGGTTGCCTCAATGCTAGCAAGGAACTCAGAGAGTTTGTAGCTCATACAGGCATTCCGGTGGCCAGTACTCTAATGGGCTTGGGTTCTTTTCCATCTTCTCATGAGAAAAGCTTGGGGATGTTGGGCATGCACGGGACTGTCTATGCCAATTATGCAGTGGATAAAGCTGATCTGTTGCTTGCTTTTGGTGTTCGCTTTGATGATAGAGTAACTGGTAAATTGGAGTCATTTGCAAGCCGTGCCAGCATTGTTCATATTGACATAGATCCTGCAGAGATTGGAAAGAACAAACAGCCTCATATCTCTTTGTGTGCAGATGTGCAAGTTGCACTCCAAGGGCTAAACTACTTAATGAAGAAGAGGGAAGACAGACCAGATTTCAATGCTTGGATAGACGAACTGGAAGAGCAGAAGATAAAATGGCCTTTAACTTATCAGAACTTTGAAGAAGCTATTCCTCCTCAACATGCCATTCAATTACTTTATGAACTGACACATGGCAATGCCATTATCACCACTGGAGTGGGTCAACATCAGATGTGGTCTGCTCAGTGGTTCAAATATGATCGGCCTCGCCAGTGGCTGACCTCAGGTGGTCTTGGTGCTATGGGCTTTGGTTTGCCTGCAGCAGTGGGAGCAGCTGTTGGAAATCCTGGGGTGACTGTGGTTGATATTGATGGGGATGGTAGCTTTATAATGAACGTTCAAGAATTGGCTACCATTAAAGTAGAAAATTTGCCAGTAAAGATTATGATTTTGAACAATCAACACCTGGGTATGGTTGTCCAATGGGAAGATCGGTTTTATAAAGCAAACAGAGCACACACATACCTTGGAGATCCATCTCAAGAATGTGAGGTCTTCCCAGATTTCTTGAAGATGGCAGAATCGTGCAACATTCCTGCAGCCCGTGTTACAGATAAAGCACATATTAGGGATGCCATTGCTACGATGTTGGAAACACCTGGACCATATTTATTGGATGTAATTGTTCCTCATCAAGAACATGTGTTGCCAATGATACCCGCTGGTGGAAGTTTCAAGGAAATCATTACTGAAGGTGATGGTAGAATGTCGTACTAGAAGTTGGGGACTTTCCTGGTTGTATCATATCATACTCGGCCTAAAATGTTGGAGCTTACTAGAAATATGGTTTTCTCTTCCATATTTGCTTTATATTCCATTACAAGCTAAGTTAAATTTCTCTAAATGTTTGTAGTAAGACATTGGTTACTGAGGCTCCTTTGAGTTATTACAGCACTTTATGGTCTGCATTTATT

Ace file of the UniGene biog3_v1.0_unigene3890 (antisense)

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene545	63/68
SustainPine v2.0	sp_v2.0_unigene30125	3/68

Source	Descriptions	Term	Type	Identity
AutoFact	Acetolactate synthase n=1 Tax=Picea sitchensis RepID=B8LK99_PICSI	-	-	92%
FL-Next	sp=Acetolactate synthase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	95%
Blast2go	acetolactate synthase	-	-	87%

Source	ECs	Term	Type	e value	Identity
Blast2go	Acetolactate synthase.	EC:2.2.1.6	-	0.0	87%

Source	KEGGs	Term	Identity
Blast2go	Valine, leucine and isoleucine biosynthesis	00290	87%
Blast2go	Butanoate metabolism	00650	87%
Blast2go	C5-Branched dibasic acid metabolism	00660	87%
Blast2go	Pantothenate and CoA biosynthesis	00770	87%
Blast2go	Biosynthesis of alkaloids derived from histidine and purine	01065	87%
Blast2go	Metabolic pathways	01100	87%
Blast2go	Biosynthesis of secondary metabolites	01110	87%

Source	GOs	Term	Type	Identity
Blast2go	response to herbicide	GO:0009635	Biological Process	87%
Blast2go	branched chain family amino acid biosynthetic process	GO:0009082	Biological Process	87%
Blast2go	flavin adenine dinucleotide binding	GO:0050660	Molecular Function	87%
Blast2go	thiamine pyrophosphate binding	GO:0030976	Molecular Function	87%
Blast2go	magnesium ion binding	GO:0000287	Molecular Function	87%
Blast2go	acetolactate synthase activity	GO:0003984	Molecular Function	87%
Blast2go	lyase activity	GO:0016829	Molecular Function	87%
Blast2go	chloroplast	GO:0009507	Cellular Component	87%

Source	InterPros	Term	Type	Identity
Sma3	Acetolactate synthase, large subunit, biosynthetic	IPR012846	-	-
Sma3	TPP-binding enzyme, conserved site	IPR000399	-	-
Sma3	Thiamine pyrophosphate enzyme, N-terminal TPP-binding domain	IPR012001	-	-
Sma3	Thiamine pyrophosphate enzyme, C-terminal TPP-binding	IPR011766	-	-
Sma3	Thiamine pyrophosphate enzyme, central domain	IPR012000	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LK99

Fln msg: your sequence is shorter than subject: 641 - 669

Fln protein: LLPVKVSQEGKIRAKRGLRHLADKTTAPIVENTLQDWSESAVELKNPSQTSVKNFPSSFTSRYADNEPRKGADVIVESLEREGVEHVFAYPGGASMEIHQALTRSKTIKNVLCRHEQGEIFAAEGYARSTGKVAVCIATSGPGATNLVSGLADALLDSIPLVAITGQVPRRMIGTDAFQETPIVEVTRSITKHNYLIMDVDDIPRVIREAFYLAASGRPGPVLVDIPKDVQQQMTVPDWNKAIKLHGYTERLPKAPQISQLEQILRLVFNSNKPVLYVGGGCLNASKELREFVAHTGIPVASTLMGLGSFPSSHEKSLGMLGMHGTVYANYAVDKADLLLAFGVRFDDRVTGKLESFASRASIVHIDIDPAEIGKNKQPHISLCADVQVALQGLNYLMKKREDRPDFNAWIDELEEQKIKWPLTYQNFEEAIPPQHAIQLLYELTHGNAIITTGVGQHQMWSAQWFKYDRPRQWLTSGGLGAMGFGLPAAVGAAVGNPGVTVVDIDGDGSFIMNVQELATIKVENLPVKIMILNNQHLGMVVQWEDRFYKANRAHTYLGDPSQECEVFPDFLKMAESCNIPAARVTDKAHIRDAIATMLETPGPYLLDVIVPHQEHVLPMIPAGGSFKEIITEGDGRMSY*

Protein Length: 642

Fln nts: TTGTTGCCAGTAAAGGTCAGTCAGGAGGGAAAAATTCGTGCAAAAAGGGGTCTTCGACATCTGGCCGACAAAACTACGGCTCCAATTGTTGAGAACACATTACAAGATTGGAGTGAATCTGCAGTGGAACTGAAGAACCCATCACAAACATCTGTCAAGAATTTTCCAAGTTCATTTACATCACGTTATGCAGATAATGAGCCAAGAAAGGGTGCAGATGTCATTGTAGAGTCTTTAGAGCGAGAGGGTGTTGAGCATGTCTTCGCTTACCCTGGAGGTGCATCTATGGAAATTCATCAGGCACTTACTCGATCCAAGACCATAAAAAATGTCCTTTGTCGCCATGAACAAGGAGAGATATTTGCAGCAGAGGGTTATGCCAGGTCTACAGGAAAGGTCGCTGTTTGCATTGCAACTTCTGGTCCTGGTGCCACCAACTTGGTAAGTGGTCTTGCTGATGCATTGCTTGACAGCATTCCTTTGGTTGCTATTACTGGTCAGGTGCCTCGGAGAATGATAGGCACAGATGCCTTTCAAGAAACTCCGATTGTGGAGGTGACTAGGTCTATAACAAAGCACAATTACTTGATCATGGATGTGGATGATATACCCCGAGTCATCAGAGAGGCCTTTTATCTTGCAGCATCTGGGAGGCCCGGACCTGTTTTAGTTGATATCCCAAAGGATGTTCAGCAGCAGATGACTGTTCCAGATTGGAACAAGGCAATAAAACTTCATGGTTATACTGAAAGACTCCCAAAGGCCCCACAGATTTCTCAGTTAGAACAGATCCTGCGCCTTGTGTTTAACTCTAATAAACCTGTCCTTTACGTTGGTGGGGGTTGCCTCAATGCTAGCAAGGAACTCAGAGAGTTTGTAGCTCATACAGGCATTCCGGTGGCCAGTACTCTAATGGGCTTGGGTTCTTTTCCATCTTCTCATGAGAAAAGCTTGGGGATGTTGGGCATGCACGGGACTGTCTATGCCAATTATGCAGTGGATAAAGCTGATCTGTTGCTTGCTTTTGGTGTTCGCTTTGATGATAGAGTAACTGGTAAATTGGAGTCATTTGCAAGCCGTGCCAGCATTGTTCATATTGACATAGATCCTGCAGAGATTGGAAAGAACAAACAGCCTCATATCTCTTTGTGTGCAGATGTGCAAGTTGCACTCCAAGGGCTAAACTACTTAATGAAGAAGAGGGAAGACAGACCAGATTTCAATGCTTGGATAGACGAACTGGAAGAGCAGAAGATAAAATGGCCTTTAACTTATCAGAACTTTGAAGAAGCTATTCCTCCTCAACATGCCATTCAATTACTTTATGAACTGACACATGGCAATGCCATTATCACCACTGGAGTGGGTCAACATCAGATGTGGTCTGCTCAGTGGTTCAAATATGATCGGCCTCGCCAGTGGCTGACCTCAGGTGGTCTTGGTGCTATGGGCTTTGGTTTGCCTGCAGCAGTGGGAGCAGCTGTTGGAAATCCTGGGGTGACTGTGGTTGATATTGATGGGGATGGTAGCTTTATAATGAACGTTCAAGAATTGGCTACCATTAAAGTAGAAAATTTGCCAGTAAAGATTATGATTTTGAACAATCAACACCTGGGTATGGTTGTCCAATGGGAAGATCGGTTTTATAAAGCAAACAGAGCACACACATACCTTGGAGATCCATCTCAAGAATGTGAGGTCTTCCCAGATTTCTTGAAGATGGCAGAATCGTGCAACATTCCTGCAGCCCGTGTTACAGATAAAGCACATATTAGGGATGCCATTGCTACGATGTTGGAAACACCTGGACCATATTTATTGGATGTAATTGTTCCTCATCAAGAACATGTGTTGCCAATGATACCCGCTGGTGGAAGTTTCAAGGAAATCATTACTGAAGGTGATGGTAGAATGTCGTACTAG___AAGTTGGGGACTTTCCTGGTTGTATCATATCATACTCGGCCTAAAATGTTGGAGCTTACTAGAAATATGGTTTTCTCTTCCATATTTGCTTTATATTCCATTACAAGCTAAGTTAAATTTCTCTAAATGTTTGTAGTAAGACATTGGTTACTGAGGCTCCTTTGAGTTATTACAGCACTTTATGGTCTGCATTTATT

Fln Alignment:

Contig3890___LPVKVSQEGKIRAKRGLRHLADKTTAPIVENTLQDWSESAVELKNPSQTSVKNFPSSFTSRYADNEPRKGADVIVESLEREGVEHVFAYPGGASMEIHQALTRSKTIKNVLCRHEQGEIFAAEGYARSTGKVAVCIATSGPGATNLVSGLADALLDSIPLVAITGQVPRRMIGTDAFQETPIVEVTRSITKHNYLIMDVDDIPRVIREAFYLAASGRPGPVLVDIPKDVQQQMTVPDWNKAIKLHGYTERLPKAPQISQLEQILRLVFNSNKPVLYVGGGCLNASKELREFVAHTGIPVASTLMGLGSFPSSHEKSLGMLGMHGTVYANYAVDKADLLLAFGVRFDDRVTGKLESFASRASIVHIDIDPAEIGKNKQPHISLCADVQVALQGLNYLMKKREDRPDFNAWIDELEEQKIKWPLTYQNFEEAIPPQHAIQLLYELTHGNAIITTGVGQHQMWSAQWFKYDRPRQWLTSGGLGAMXXXXXXXXXXXXXXXXXXXVDIDGDGSFIMNVQELATIKVENLPVKIMILNNQHLGMVVQWEDRFYKANRAHTYLGDPSQECEVFPDFLKMAESCNIPAARVTDKAHIRDAIATMLETPGPYLLDVIVPHQEHVLPMIPAGGSFKEIITEGDGRMSY
B8LK99_________________LPVKVSHEGTIRTKRGLRLQADKTTAPIVERTSQEWSESAVELKDPSQTSVKSLPSSFTSRYADSEPRKGADVIVEALEREGVEHVFAYPGGASMEIHQALTRSKTIKNVLCRHEQGEVFAAEGYARSTGKVAICIATSGPGATNLVSGLADALLDSIPLVAITGQVPRRMIGTDAFQETPIVEVTRSITKHNYLIMDVDDIPRVIREAFYLAASGRPGPVLVDIPKDVQQQMTVPDWNKAIKLHGYMERLPKAPQISQLEQILRLVFNSKKPVLYVGGGCLNASQELREFVDHTGIPVASTLMGLGSFPSSHEKSLGMLGMHGTVYANYAVDKADLLLAFGVRFDDRVTGKLESFASRASIVHIDIDPAEIGKNKQPHISLCADVQLALQGLNYLMKKREDRPDFYAWIDELEEQKTKWPLTYQNFEEAIPPQHAIQLLYELTHGNVIITTGVGQHQMWSAQWFKYDRPRQWLTSGGLGAMGFGLPAAVGAAVGNPGVTVVDIDGDGSFMMNVQELATIKVENLPVKIMILNNQHLGMVVQWEDRFYKANRAHTYLGDPSQECEIFPDFLKMAESCNIPAGRVTDKAHIRDAIATMLETPGPYLLDVIVPHQEHVLPMIPAGGSFKEIITEGDGRISY

SNPs (tot: 85)

Position	A %	C %	G %	T %	Probability
388	20	80			0.995493
390		80		20	0.995493
392	80			20	0.995493
506	20	80			0.995493
509			80	20	0.995493
510	20		60	20	0.99255
511		25		75	0.996473
512	25		75		0.996473
528		83		16	0.994512
550	12			87	0.992554
551	12	87			0.992554
553	87		12		0.992554
554		87		12	0.992554
596	11	88			0.923564
599		88	11		0.923564
600		88		11	0.923564
608		11		88	0.923564
668	88			11	0.923564
670		88		11	0.923564
694	16		83		0.999991
704			88	11	0.923564
705	11		88		0.923564
706		88		11	0.923564
707	11		88		0.923564
709	11		88		0.923564
710	22		77		0.999999
716		88		11	0.923564
731			87	12	0.992554
800	12		87		0.992554
803	85		14		0.993533
824		16	83		0.994512
826	83			16	0.994512
923	20			80	0.995493
924	20	80			0.995493
926			20	80	0.995493
927		75		25	0.996473
930	25			75	0.996473
931	75	25			0.996473
933			75	25	0.996473
939	25			75	0.996473
942		75		25	0.996473
1177	14			85	0.993533
1181			85	14	0.993533
1197	66			33	1.0
1211		16	83		0.994512
1213			83	16	0.994512
1240		20	80		0.995493
1241	20	80			0.995493
1243	80			20	0.995493
1329	25		75		0.996473
1332		75	25		0.996473
1427	16		83		0.994512
1428	16	83			0.994512
1431	83			16	0.994512
1445		16		83	0.994512
1448		80		20	0.995493
1547	16			83	0.994512
1548		16		83	0.994512
1549	16		83		0.994512
1596	20			80	0.995493
1597	20		80		0.995493
1601		80		20	0.995493
1602	16			83	0.994512
1606		83		16	0.994512
1615	16		83		0.994512
1630	83			16	0.994512
1656			85	14	0.993533
1657		71	14	14	0.984337
1658	85			14	0.993533
1666	85			14	0.993533
1683	85		14		0.993533
1689		33		66	1.0
1793		16		83	0.994512
1794	16			83	0.994512
1845	16	83			0.994512
1847		83		16	0.994512
1855	62		37		1.0
1907	14		85		0.993533
1910		85		14	0.993533
1911		83		16	0.994512
1931	20		80		0.995493
1935	80			20	0.995493
1951	75	25			0.996473
1952	25	75			0.996473
1973	33		66		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain