SustainPineDB

Assembly Name: biogeco3 v1.0

UniGene Name: biog3_v1.0_unigene3661

Length: 1250 nt

UniGene Fasta

Ace file of the UniGene biog3_v1.0_unigene3661

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene4889	69/69

Source	Descriptions	Term	Type	Identity
AutoFact	NAD-dependent epimerase/dehydratase n=1 Tax=Zea mays RepID=B6T962_MAIZE	-	-	77%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	95%
Blast2go	nad-dependent epimerase dehydratase	-	-	88%

Source	ECs	Term	Type	e value	Identity
Blast2go	3-beta-hydroxy-Delta(5)-steroid dehydrogenase.	EC:1.1.1.145	-	0.0	88%

Source	KEGGs	Term	Identity
Blast2go	Steroid hormone biosynthesis	00140	88%
Blast2go	Biosynthesis of terpenoids and steroids	01062	88%
Blast2go	Biosynthesis of plant hormones	01070	88%
Blast2go	Metabolic pathways	01100	88%

Source	GOs	Term	Type	Identity
Blast2go	defense response to bacterium	GO:0042742	Biological Process	88%
Blast2go	steroid biosynthetic process	GO:0006694	Biological Process	88%
Blast2go	response to abscisic acid stimulus	GO:0009737	Biological Process	88%
Blast2go	oxidation-reduction process	GO:0055114	Biological Process	88%
Blast2go	3-beta-hydroxy-delta5-steroid dehydrogenase activity	GO:0003854	Molecular Function	88%
Blast2go	copper ion binding	GO:0005507	Molecular Function	88%
Blast2go	plasma membrane	GO:0005886	Cellular Component	88%
Blast2go	vacuolar membrane	GO:0005774	Cellular Component	88%
Blast2go	chloroplast stroma	GO:0009570	Cellular Component	88%
Blast2go	thylakoid	GO:0009579	Cellular Component	88%
Blast2go	apoplast	GO:0048046	Cellular Component	88%

Source	InterPros	Term	Type	e value	Identity
Sma3	NAD(P)-binding domain	IPR016040	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NPJ0

Fln msg:

Fln protein: MGDSATTVLVTGAGGRTGALVYKKLKERAGQFVSRGFVRTDESKQKIGGGDDVFVGDIRQPETLTPVVQGVDALVIVTSAVPKMKPGFDPSKGGRPEFYFEDGCYPEQVDWIGQKNQIDAAKAVGAKHIVLVGSMGGTNPNHPLNSLGNGKILIWKRKAEQYLADSGIPYTIIRAGGLVDKEGGQRQLLVGKDDELLASETKSIPRADVAEVCVQALLIEEAKNKAFDIVSKPEEAGSPTTDFKALFSQVSTKF*

Protein Length: 256

Fln nts: CTTCCGACCCACTATCATTCATTTTCACCTCTCTCATGTACAACGAGGAACTCGGACTCCTTCAATTCTACTTATAGATTGCCAAATTCTTCTCGCACGCGGCATCGTTGCAGTGTAAGATTATTTCCAAAACTCTCTACTGCTGCAAATAGCGATTTGGGTTCCAGTGGGCAGTTGAACTTTGATTGGATATCGTCTTTGTTGCTGGGCTGCCCCTTACTGTGCGGCCAGAGGTCTTCGAAGCCCGTGACACAAGCTGCAGCATTATCAGTAGCAGCAGCA_-_ATGGGTGATTCTGCGACTACTGTGCTGGTTACTGGTGCTGGCGGTAGAACAGGTGCATTGGTATACAAGAAGTTGAAAGAAAGAGCAGGGCAGTTTGTGAGTAGAGGTTTTGTTAGGACAGATGAAAGCAAACAGAAAATTGGTGGTGGAGACGACGTGTTTGTAGGTGATATCAGACAACCTGAAACTCTGACTCCAGTCGTCCAAGGCGTTGATGCACTGGTCATTGTTACAAGTGCCGTGCCCAAGATGAAGCCCGGCTTTGATCCCTCCAAGGGAGGACGCCCAGAGTTTTATTTCGAAGATGGATGCTATCCCGAGCAGGTTGATTGGATTGGACAAAAGAATCAGATTGATGCAGCCAAAGCTGTGGGAGCTAAGCATATAGTCTTAGTTGGATCCATGGGCGGGACAAATCCTAATCATCCATTGAATTCTCTAGGGAATGGGAAAATTCTGATATGGAAGCGGAAGGCTGAGCAATATCTTGCAGACTCTGGTATTCCTTATACGATAATCAGAGCTGGAGGCTTGGTTGACAAGGAAGGTGGCCAGCGGCAATTACTGGTAGGGAAAGATGATGAACTTCTTGCCAGTGAGACCAAATCAATACCCAGAGCTGATGTAGCAGAGGTCTGTGTACAGGCATTACTTATTGAAGAGGCAAAGAACAAGGCATTTGATATTGTATCAAAACCAGAAGAAGCAGGATCACCAACAACAGATTTCAAGGCCCTTTTCAGTCAAGTGTCCACTAAATTTTGA___TTCTTGCAGAGTTGGCTTTTTGGCATTGTGGCTTGGCCATATTATGTGGATGCTTATTTCAATGCTCGACTTTGTTAGTATAAACATTTAAAACCACACAAATAGACGTTCTGTCTAATGTTTTTCTAAATGGTTCAATTTCTGGTACGAGTTTCTATTTCATAATGCAAGTCTTATTGCGGAGCAATAAGACTTGCATTATG

Fln Alignment:

Contig3661___TTVLVTGAGGRTGALVYKKLKERAGQFVSRGFVRTDESKQKIXXXXXXXXXXIRQPETLTPVVQGVDALVIVTSAVPKMKPGFDPSKGGRPEFYFEDGCYPEQVDWIGQKNQIDAAKAVGAKHIVLVGSMGGTNPNHPLNSLGNGKILIWKRKAEQYLADSGIPYTIIRAGGLVDKEGGQRQLLVGKDDELLASETKSIPRADVAEVCVQALLIEEAKNKAFDIVSKPEEAGSPTTDFKALFSQVSTKF
A9NPJ0_________________TTVLVTGAGGRTGELVYKKLKERGGQFVSRGFVRTEESKQKIGGGDDVFVGDVRQPETLTPVFQGVDALVIVTSAVPKMKPGFDPSKGGRPEFYFEDGCYPEQVDWIGQKNQIDAAKAVGAKQIVLVGSMGGTNPNHPLNSLGNGKILIWKRKAEQYLADSGIPYTIIRAGGLVDKEGGLRQLLVGKDDELLATETKSVPRADVAEVSVQALLIEEAKNKAFDLASKPEEAGSPTTDFKALFSQVSTKF

SNPs (tot: 19)

Position	A %	C %	G %	T %	Probability
306	16		83		0.994512
307	16	83			0.994512
354	14		85		0.993533
408	85	14			0.993533
410			85	14	0.993533
411		85		14	0.993533
471		11		88	0.923564
944			76	23	1.0
945	76			23	1.0
968		28		71	1.0
972	20		80		0.995493
973		20		80	0.995493
976		80		20	0.995493
987			75	25	0.996473
988	75			25	0.996473
994			66	33	0.997452
995			66	33	0.997452
1025	20			80	0.995493
1028			80	20	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain