SustainPineDB

Assembly Name: biogeco3 v1.0

UniGene Name: biog3_v1.0_unigene3607

Length: 1245 nt

UniGene Fasta

Ace file of the UniGene biog3_v1.0_unigene3607

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene6599	23/25
SustainPine v2.0	sp_v2.0_unigene17808	1/25
SustainPine v2.0	sp_v2.0_unigene69375	1/25

Source	Descriptions	Term	Type	Identity
AutoFact	putative C-4 sterol methyl oxidase [Arabidopsis thaliana]	-	-	67%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	46%
Blast2go	c-4 sterol methyl oxidase	-	-	80%

Source	GOs	Term	Type	Identity
Blast2go	fatty acid biosynthetic process	GO:0006633	Biological Process	80%
Blast2go	sterol biosynthetic process	GO:0016126	Biological Process	80%
Blast2go	oxidation-reduction process	GO:0055114	Biological Process	80%
Blast2go	iron ion binding	GO:0005506	Molecular Function	80%
Blast2go	4,4-dimethyl-9beta,19-cyclopropylsterol-4alpha-methyl oxidase activity	GO:0080064	Molecular Function	80%
Blast2go	zinc ion binding	GO:0008270	Molecular Function	80%
Blast2go	integral to membrane	GO:0016021	Cellular Component	80%
Blast2go	endoplasmic reticulum	GO:0005783	Cellular Component	80%

Source	InterPros	Term	Type	e value	Identity
Sma3	Fatty acid hydroxylase	IPR006694	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NUC0

Fln msg: your sequence is longer than subject: 304 - 262

Fln protein: MLPFETLEQAELELGRALSYWETLWFRYSARIPDSILYYHNIVFLLIVFTIVPLPLALLDTKKIKSLQKYKIQPKIETPKSEMLYCYKAVLFVFFAVVGPLQLTSYPLIKLVGIRTGLPLPSLWEIFAQLAVYFMVEDYGNYWIHRWLHCKWGYEKIHHVHHEFTAPMGFAAPYAHWSEVLILGIPTFVGPAIAPGHMITFWFWVVLRQVEAIETHSGYDFPWTLTKLIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYLYGTDKGYRYHKEHLLKARELNIVEADDFKKKTAMEQFPISL*

Protein Length: 305

Fln nts: TTTCGATTCATTCCCTAGATCTGGTCAGTTGGGGTGCTGTGGCAAAGCTCCTGACCATTTTACA_-_ATGCTGCCCTTTGAAACATTAGAGCAGGCAGAGCTCGAGCTGGGCAGGGCCCTCTCGTATTGGGAAACTCTCTGGTTCAGATATTCAGCCAGAATACCTGACAGCATTCTCTACTACCACAACATCGTGTTCCTCCTCATCGTTTTCACAATCGTTCCCCTGCCCTTGGCTCTTCTAGACACCAAGAAGATCAAGAGCTTGCAGAAGTATAAGATTCAGCCCAAAATTGAGACACCTAAGTCTGAAATGCTGTATTGCTACAAGGCTGTCTTGTTTGTGTTCTTCGCAGTTGTGGGACCTCTGCAGCTGACATCGTATCCTCTGATCAAGCTCGTGGGTATCAGAACAGGCCTGCCATTGCCTTCCCTGTGGGAAATTTTTGCGCAGCTTGCAGTTTATTTCATGGTTGAAGACTATGGCAACTATTGGATACACAGGTGGCTACACTGCAAATGGGGCTATGAGAAGATCCATCATGTTCACCATGAGTTCACTGCTCCAATGGGTTTTGCTGCTCCATATGCACATTGGTCAGAGGTGTTGATATTGGGGATCCCTACGTTTGTCGGACCGGCAATTGCTCCAGGACATATGATTACATTCTGGTTCTGGGTTGTGCTGCGCCAAGTGGAAGCTATTGAAACTCACAGCGGATATGACTTTCCGTGGACTCTTACCAAATTAATTCCTTTCTATGGAGGCGCGGAGTATCATGACTACCATCATTATGTAGGAGGACAAAGTCAAAGCAACTTTGCCTCAGTGTTCACATACTGTGATTATTTATACGGGACTGATAAGGGTTACCGCTATCATAAGGAGCATCTTTTGAAGGCACGTGAGTTGAATATAGTTGAAGCAGATGATTTTAAGAAAAAAACGGCAATGGAGCAGTTTCCGATAAGTTTGTAA___AAGATCAGAAGTCTCAGTAGGCAGGATAGATGCTTCTTTCCTCCCTCGTGTGTTCTTTGCGTGTGTTTCACATGTAGATGGGATAACTCTCATGCCTTTGATATTGCCTCTGTCATACAAGATTGGTGACTGGAATTCTCAAAAGGGCGGGGCATCATGTTTTCAGATTCTGAATGTGGTGTGCAGAAAAGAAGATAGAACATGATGATGTTTATTTGAAATGCAAGAAGACCAATGTTTGTCATTGATTTAAAATGCGAAAGGACATT

Fln Alignment:

Contig3607___VFTIVPLPLALLDTKKIKSLQKYKIQPKIETPKSEMLYCYKAVLFVFFAVVGPLQLTSYPLIKLVGIRTGLPLPSLWEIFAQLAVYFMVEDYGNYWIHRWLHCKWGYEKIHHVHHEFTAPMGFAAPYAHWSEVLILGIPTFVGPAIAPGHMITFWFWVVLRQVEAIETHSGYDFPWTLTKLIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYLYGTDKGYR
A9NUC0_________________IFFLSGLPFIILETTGL--LSKYKIQSKTNTPAAQEK-CITRLLLYHFCVNLPVMVVSYPVFRFMGMRSVLPLPSWKVVVSQLVCYFILEDFVFYWGHRILHTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIIGPAITGPHLFTLWLWMSLRVLETVEAHCGYDFPWSFSKIFPLYGGADFHDYHHRLLYTKSGNYSSTFTYMDWLFGTDKGYR

SNPs (tot: 17)

Position	A %	C %	G %	T %	Probability
504	25		75		0.996473
505		25	75		0.996473
506	25	75			0.996473
508	75			25	0.996473
535	33	66			0.997452
539		66		33	0.997452
758		12		87	0.992554
761			87	12	0.992554
812	87			12	0.992554
814		87		12	0.992554
832		87		12	0.992554
915		14		85	0.993533
1073	33			66	0.997452
1074	33			66	0.997452
1164		50	50		1.0
1216		25	75		0.996473
1217	25			75	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain