SustainPineDB

Assembly Name: biogeco3 v1.0

UniGene Name: biog3_v1.0_unigene3456

Length: 1138 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene biog3_v1.0_unigene3456 (antisense)

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene187	22/22

Source	Descriptions	Term	Type	Identity
AutoFact	RecName: Full=Glutamate--cysteine ligase, chloroplastic; AltName: Full=Gamma-ECS; Short=GCS; AltName: Full=Gamma-glutamylcysteine synthetase; Flags: Precursor gb\|AAB71230.1\| gamma-glutamylcysteine synthetase [Solanum lycopersicum]	-	-	81%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	95%
Blast2go	gamma-glutamylcysteine synthetase	-	-	89%

Source	ECs	Term	Type	e value	Identity
Blast2go	Glutamate--cysteine ligase.	EC:6.3.2.2	-	0.0	89%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Glutathione metabolism	00480		0.0	89%
Blast2go	Metabolic pathways	01100		0.0	89%

Source	GOs	Term	Type	Identity
Blast2go	glutathione biosynthetic process	GO:0006750	Biological Process	89%
Blast2go	glutamate-cysteine ligase activity	GO:0004357	Molecular Function	89%
Blast2go	ATP binding	GO:0005524	Molecular Function	89%
Blast2go	chloroplast	GO:0009507	Cellular Component	89%

Source	InterPros	Term	Type	e value	Identity
Sma3	Glutamate--cysteine ligase, GCS2	IPR006336	-	0.0	-
Sma3	Glutamate--cysteine ligase, plant	IPR011556	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NTB1

Fln msg: your sequence is longer than subject: 315 - 209

Fln protein: MLPFVFDDTFGFESYVDYALDVPMYFVYRNKKYVDCSGMSFKDFMKGKLPSLPGELPNLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPALWVGLLYDETSLQSALDITKDWTLEEMQMLRNKVPKMGLKTPFRDSLLRHVAEDILQLAKDGLQRRGFKESGFLNEVAEVVRTGVTPAERLLDLYHGKWANSVDPVLKNFFTNCHHVGQLVGTCYVGTCICKDRGWTNCCFYKLMLLTRCSKSFIIIVVLCDVLLHYLVVEQYSLIMNKASSICNIKRNVELHYNSVCMQLRIGESKTYAVNYCLSS*

Protein Length: 316

Fln nts: GCTATAGAAGCCATATTTGGACAGATACTGACAACAGTCGTTGTGGA_-_ATGCTTCCGTTTGTTTTTGATGATACATTTGGGTTTGAAAGTTATGTGGATTATGCTCTAGATGTTCCCATGTACTTTGTATATCGGAATAAGAAATATGTTGATTGCAGTGGCATGTCTTTCAAAGATTTCATGAAGGGTAAATTACCATCGTTACCTGGTGAATTGCCCAATCTTAATGACTGGGAGAATCATCTTACTACCATTTTCCCTGAGGTGAGGCTTAAGAGGTACTTGGAGATGAGAGGGGCAGATGGAGGTCCTTGGAGAAGATTGTGTGCTTTACCGGCATTATGGGTAGGGTTGCTGTATGATGAAACCTCACTTCAGTCAGCATTGGACATCACAAAAGACTGGACACTTGAAGAAATGCAGATGTTAAGGAATAAGGTCCCAAAGATGGGTTTGAAGACTCCTTTCCGAGACTCCTTGTTGAGGCATGTAGCAGAAGATATTTTGCAACTGGCAAAAGATGGTTTGCAAAGAAGAGGCTTCAAGGAAAGTGGGTTTTTAAATGAAGTAGCTGAAGTAGTTAGAACAGGTGTTACGCCAGCGGAAAGACTTCTAGACTTGTACCATGGAAAGTGGGCTAACAGTGTGGACCCTGTTTTGAAGAACTTCTTTACTAACTGCCACCATGTAGGCCAACTTGTGGGTACATGCTACGTGGGAACTTGCATCTGTAAAGATCGAGGATGGACGAACTGCTGCTTCTATAAATTGATGCTTCTTACGAGGTGTTCCAAAAGCTTCATTATCATTGTAGTCCTCTGCGATGTTCTATTACATTATCTAGTAGTTGAGCAGTATTCTCTTATAATGAATAAGGCATCGTCCATTTGTAATATCAAAAGAAATGTAGAGTTGCACTACAACTCTGTTTGTATGCAATTAAGAATTGGTGAATCTAAAACTTACGCGGTAAACTATTGTCTGTCCAGTTGA___GCAAGGCCTTTCCTCAAAGAAAATTTTGGTGGTAGGGATGACTTTTGACCTGAGTGCTTCAGTTTTGAAGTACTAATCCCCTATTTGGTGACGTTATGTACTTTTTGGACATTTTAGTGATATATTAAAGAGGGTGGCTATACATT

Fln Alignment:

Contig3456___MLPFVFDDTFGFESYVDYALDVPMYFVYRNKKYVDCSGMSFKDFMKGKLPSLPGELPNLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPALWVGLLYDETSLQSALDITKDWTLEEMQMLRNKVPKMGLKTPFRDSLLRHVAEDILQLAKDGLQRRGFKESGFLNEVAEVVRTGVTPAERLLDLYHGKWANSVDPVLK
A9NTB1_________________MLPFVFDDTFGFESYVEYALDVPMYFVYRKKKYVDCSGMSFKDFMKGKLPSLPGELPNLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPALWVGLLYDETSLQSVLDITKDWTLEEMQMLRNKVPKMGLKTPFRDSLLRHVAEDILQLAKEGLQRRGCKESGFLNEVAEVVRTGITPAERLLDLYHGKWGNCVDPVFE

SNPs (tot: 25)

Position	A %	C %	G %	T %	Probability
132	33		66		0.997452
136			66	33	0.997452
400	33			66	0.997452
401	33	66			0.997452
402			66	33	0.997452
403	66		33		0.997452
638	80			20	0.995493
639	80		20		0.995493
834	16	83			0.994512
835		83		16	0.994512
836			16	83	0.994512
837		83		16	0.994512
842	14			85	0.993533
843	14		85		0.993533
844	12	75		12	0.975346
845	12	75	12		0.975346
846		75		25	1.0
847		87	12		0.992554
911	12		87		0.992554
913		87		12	0.992554
992	66		33		1.0
1002	16			66	0.988813
1003		16		83	0.994512
1100	66			33	0.997452
1101		66		33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain