SustainPineDB

Assembly Name: biogeco3 v1.0

UniGene Name: biog3_v1.0_unigene66

Length: 1700 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene biog3_v1.0_unigene66 (antisense)

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene28226	28/29

Source	Descriptions	Term	Type	Identity
AutoFact	leucine-rich repeat protein kinase-like protein [Arabidopsis thaliana] gb\|AAD24639.1\| putative receptor-like protein kinase [Arabidopsis thaliana] gb\|ACN59305.1\| leucine-rich repeat receptor-like protein kinase [Arabidopsis thaliana] gb\|AEC09269.1\| leucin	-	-	54%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	64%
Blast2go	probable leucine-rich repeat receptor-like protein kinase at1g68400-like	-	-	71%

Source	GOs	Term	Type	e value	Identity
Blast2go	protein binding	GO:0005515	Molecular Function	0.0	71%
Blast2go	plasma membrane	GO:0005886	Cellular Component	0.0	71%

Source	InterPros	Term	Type	Identity
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Serine-threonine/tyrosine-protein kinase catalytic domain	IPR001245	-	-
Sma3	Leucine-rich repeat	IPR001611	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	Leucine-rich repeat-containing N-terminal, type 2	IPR013210	-	-
Sma3	SKG6/AXL2 alpha-helix transmembrane domain	IPR014805	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-
Sma3	Formate C-acetyltransferase glycine radical, conserved site	IPR019777	-	-
Sma3	Peroxidases heam-ligand binding site	IPR019793	-	-

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LN40

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 18 aas, your sequence is shorter than subject: 321 - 340

Fln protein: MDFSGGAQTPEMERSKLVFFDRKKQFELEDLLRASAEMLGKGSFGTAYKAVLDDGCVVAVKRLKDVNPSGKKEFDQHMELIGKLRHPHIVRLRAYYYAKEEKLLVYDYLPNGSLYSLLHGNRGPGRTPLDWTTRISLVLGAARGLAYIHEEYGSLKIPHGNIKSSNILLDKNGVACISDFGLALLLNPSHIHASSRYVGYRAPEQAETKKLSQKADVYSFGVLLLEVLTGKAPMHYHSQEEGVDLPKWVQSIVREEWTAEVFDLELMRYKNIEEEMVSMLQIALSCVSQSPEQRPKMGEVVRLIEDIRVEQSPLGEWDES*

Protein Length: 322

Fln nts: AATGTAGTCTTCTTCATTTCGCTTTCGGGCCATGAGTTACGAGGCCCTATCGATCCTCTTTCTGCGCTCGACCAGCTCCGGGTTCTTGATCTTCAGGGAAATCGGCTCAACGGCAGCTTGGATCCATTGTCAAATTGCTACAACCTCAAATTGCTCTACTTGGCTTCCAATGACTTCTCGGGTCTGATTCCTGCTGATCTTGCTCGGCTCAGGCGTCTGGTGAGGCTCGACGCTTCCGATAACAATCTCCGCGGATCGATTCCGCCGGTGATACGGAACCTTACGCGGCTGGTAACTCTGCGCCTGCAGAACAACATTCTGTCAGGGCCGTTGCCGGATCTCAGCTTCTTGCGGGACTTAAATGTTTCCAATAATGAACTTTCTGGCCAAGTACCCCGCCAGCTTCTGGAGAAGTTCCCGGCTTCGGCCTTCGCAGGCAATGCTGGGCTCTGCGGACGGAGTCCTTTTCCGGCCTGTACCATTCCGCCACCGGTGGCTGTAGTGGTGCCGTCGAATCCGAGCTCCGACCCGGGATCGAGTACTCCCGTGGACACGAGATCCAAAACTAGGGCTCTCAGCACGGGGGCCGTCATTGCCATTGTGGTCGGTGATGCTGCCGTTCTGATTTTGATAACGCTCATTTTTCTGGTGTATTACTGGAAGAAATACGTCAGGGAGGCCGAGGTTGCTGGAAAAACGGCCTCCGAGAAG_-_ATGGATTTCTCCGGTGGCGCGCAGACGCCAGAGATGGAGAGGAGTAAACTTGTGTTCTTTGATAGGAAGAAGCAGTTTGAGTTGGAGGATCTGCTGAGGGCGTCTGCCGAGATGTTGGGGAAGGGGAGCTTTGGGACTGCTTATAAAGCCGTTCTTGATGATGGCTGTGTTGTTGCTGTCAAGAGATTAAAGGACGTTAATCCTTCTGGGAAGAAGGAATTCGATCAGCACATGGAGTTGATTGGGAAATTGAGGCATCCCCACATAGTTCGTCTCAGAGCTTATTATTATGCAAAAGAGGAGAAGCTTCTGGTTTATGATTATCTTCCCAATGGGAGCTTGTATTCTCTTCTACACGGGAACAGAGGTCCAGGAAGAACACCATTGGACTGGACTACAAGAATCAGTCTAGTCTTAGGAGCTGCTCGAGGTCTTGCTTACATCCATGAAGAATATGGGTCTTTGAAGATTCCACATGGGAATATCAAATCCTCCAATATTCTCTTGGACAAGAATGGTGTTGCATGCATTTCTGATTTTGGGTTGGCCCTGCTCCTGAACCCCTCGCATATTCATGCTTCATCTAGATATGTAGGGTACAGAGCACCTGAGCAGGCAGAAACCAAAAAATTGTCCCAAAAGGCCGATGTGTACAGCTTTGGTGTGTTACTCCTTGAGGTCTTGACTGGAAAAGCTCCTATGCACTATCATTCGCAAGAAGAAGGTGTTGATCTTCCAAAATGGGTTCAGTCCATTGTCCGGGAAGAATGGACTGCTGAAGTGTTCGACCTTGAATTGATGAGGTACAAGAACATTGAGGAGGAGATGGTTTCGATGTTGCAGATTGCTTTGAGTTGTGTTTCACAGTCACCCGAACAACGTCCTAAGATGGGTGAAGTTGTTAGGTTGATTGAGGACATCAGAGTTGAGCAATCTCCTTTGGGTGAATGGGATGAGTCTTAACTCTGTTTCTCCTTCTGCCTCACATT

Fln Alignment:

Contig66___GGAQTPEMERSKLVFFDRKKQ-FELEDLLRASAEMLGKGSFGTAYKAVLDDGCVVAVKRLKDVNPSGKKEFDQHMELIGKLRHPHIVRLRAYYYAKEEKLLVYDYLPNGSLYSLLHGNRGPGRTPLDWTTRISLVLGAARGLAYIHEEYGSLKIPHGNIKSSNILLDKNGVACISDFGLALLLNPSHIHASSRYVGYRAPEQAETKKLSQKADVYSFGVLLLEVLTGKAPMHYHSQEEGVDLPKWVQSIVREEWTAEVFDLELMRYKNIEEEMVSMLQIALSCVSQSPEQRPKMGEVVRLIEDIR
B8LN40_______________GSAQ--EAERNKLVFFEGSQYTFDLEDLLRASAEVLGKGSVGTAYKAVLEDGTTVVVKRLKDV-AANRKDFEQQMELVGRIRHRNLVPLRAFYYSKDEKLLVYDYMPTGSLSALLHGSRGSGRTPLDWDTRMRIALGAARGISHIHEEGGG-KFTHGNIKSSNVLLTTDLDGCVSDFGLVPLFSAAA--AANRIAGYRAPEVIETRKVTQKSDVYSFGVLLLELLTGKAPNQASLNDEGIDLPRWVQSVVREEWTAEVFDVELMRYQNIEEEMVQLLQIAMACVAAVPDQRPRMQDVVKMIEDMR

SNPs (tot: 19)

Position	A %	C %	G %	T %	Probability
612	33			66	0.997452
1134	25		75		0.996473
1135	25	75			0.996473
1136		75		25	0.996473
1137		75	25		0.996473
1213	33	66			1.0
1214		66		33	1.0
1216			66	33	1.0
1292		83		16	0.994512
1293			16	83	0.994512
1315	14	85			0.993533
1316	14			85	0.993533
1319			85	14	0.993533
1332	14			85	0.993533
1333		14		85	0.993533
1342		85		14	0.993533
1343	85		14		0.993533
1512			90	10	0.90298
1513		90		10	0.90298

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain