SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene3784

Length: 1905 nt

Origin of reads: Biogeco_1 Biogeco_2 Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene3784 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Ribonuclease III, putative (EC:3.1.26.3); K11592 endoribonuclease Dicer [EC:3.1.26.-]	-	-	53%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	66%
Blast2go	dicer-like protein	-	-	67%

Source	ECs	Term	Type	e value	Identity
AutoFact	Hydrolases, Acting on ester bonds, Endoribonucleases producing 5'-phosphomonoesters.	EC:3.1.26.-	-	0.0	53%

Source	GOs	Term	Type	Identity
Blast2go	embryonic pattern specification	GO:0009880	Biological Process	67%
Blast2go	suspensor development	GO:0010098	Biological Process	67%
Blast2go	vegetative phase change	GO:0010050	Biological Process	67%
Blast2go	maintenance of DNA methylation	GO:0010216	Biological Process	67%
Blast2go	production of lsiRNA involved in RNA interference	GO:0010599	Biological Process	67%
Blast2go	mRNA cleavage involved in gene silencing by miRNA	GO:0035279	Biological Process	67%
Blast2go	vegetative to reproductive phase transition of meristem	GO:0010228	Biological Process	67%
Blast2go	production of miRNAs involved in gene silencing by miRNA	GO:0035196	Biological Process	67%
Blast2go	production of ta-siRNAs involved in RNA interference	GO:0010267	Biological Process	67%
Blast2go	virus induced gene silencing	GO:0009616	Biological Process	67%
Blast2go	cytokinesis	GO:0000910	Biological Process	67%
Blast2go	protein binding	GO:0005515	Molecular Function	67%
Blast2go	double-stranded RNA binding	GO:0003725	Molecular Function	67%
Blast2go	nuclear dicing body	GO:0010445	Cellular Component	67%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Double-stranded RNA-binding	IPR001159	-	0.0	67%
Blast2go	Double-stranded RNA-binding-like	IPR014720	-	0.0	67%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LN71

Fln msg: Overlapping hits, possible frame ERROR between 121 and 111, your sequence is shorter than subject: 561 - 685

Fln protein: LPDVEKTIHYFEGDNAMAEMDSLSVKCTKRHHLLHMxxxxxDVVEALIGAYLVDSGFPAALAFLKWMGIEIDFDASLVGEACLQSKNNLSLIKCIDIDELERLLGYNFIHKGLLLEAFAHPSFNEHNGGCYQRLEFLGDAVLDYIITSYLYSVYPDMKPGYLTDMRSMTVNNESFASVAVRHGLYMYLIQKSDSLSSAVKKYVDFMKSLLTRDRDSYDEPEPKCPKVLGDIVESFAGALVVDTGFDLECVWKLVLRILEPIVTPETLCIQPVRELSEVCQHHNLTLDYRKVPKGNGFLVEANVSGKKVSGFVGGTGTRTSIKDAKKVASKDALLKLKGLD*NIHINLEVYQNMSERLCSTCR*EKAEKATSNIIITERLEEHKFFLLELLVPVICMVLEALQSLFHLARHHHRRMHRQSVSSLSCFQLP*QKMTCPQLIRTRRLIKNV*TVITIMT*LIRLSISGHLKAGTQI*TSFWDLQNQASMSFVKSEAGKCLSGFATKRKACHTKKALHIWLC*NCLKTFVLSAWENPRRTRGWQWNPLHVVHYGGLILKVTCSI*

Protein Length: 562

Fln nts: ACTTCCAGATGTGGAGAAGACAATTCACTATTTTGAAGGAGATAATGCAATGGCAGAAATGGATTCTTTGAGTGTAAAGTGCACGAAACGGCATCACTTGTTACATATGxxxxxxxxxxxxxxxGATGTAGTGGAGGCTTTGATTGGAGCATACTTAGTAGACAGTGGCTTTCCAGCAGCTTTGGCATTTCTAAAGTGGATGGGAATAGAAATTGATTTTGATGCTTCTCTCGTAGGAGAAGCTTGCTTACAAAGTAAGAATAATTTATCGCTCATTAAATGCATTGACATTGATGAGTTGGAAAGGTTGCTAGGATACAATTTTATTCATAAAGGTCTGCTCTTGGAGGCATTCGCACATCCTTCATTTAATGAACATAATGGAGGGTGTTATCAGCGTCTTGAATTCCTTGGAGATGCTGTGCTGGATTACATTATAACTTCTTACTTGTATTCGGTCTATCCAGACATGAAGCCGGGCTATTTGACAGACATGAGATCAATGACTGTCAACAACGAGTCTTTCGCTTCTGTGGCAGTTCGCCATGGTCTATATATGTATCTTATTCAGAAGTCAGATAGTCTTTCTTCTGCAGTTAAAAAGTATGTGGATTTCATGAAGTCTTTACTTACCAGAGACAGAGATTCATATGATGAGCCTGAACCAAAATGTCCTAAGGTGCTTGGGGATATAGTGGAGTCATTTGCTGGAGCTTTGGTGGTGGATACAGGCTTTGATTTAGAATGTGTTTGGAAATTAGTGCTCAGAATCTTGGAACCAATAGTCACCCCTGAAACCCTATGCATTCAACCTGTAAGGGAACTTAGTGAGGTTTGCCAACACCATAATTTGACGTTGGACTACAGAAAAGTGCCAAAGGGAAATGGATTTTTAGTTGAAGCAAATGTAAGTGGAAAAAAGGTAAGCGGATTTGTGGGTGGTACTGGCACAAGGACAAGTATAAAAGATGCTAAGAAAGTTGCTTCAAAGGATGCATTACTAAAGCTGAAGGGTTTGGATTAGAACATCCACATAAATTTAGAAGTTTATCAGAACATGTCGGAAAGGCTCTGCAGTACTTGTCGGTAAGAAAAAGCAGAAAAAGCAACATCAAACATTATAATCACAGAGAGACTGGAAGAACATAAATTTTTCCTTCTCGAGCTTCTTGTTCCCGTGATTTGCATGGTGTTAGAAGCACTTCAGAGTTTATTCCACCTGGCACGGCATCACCACAGAAGAATGCATCGACAATCTGTGAGCAGCCTCAGCTGCTTTCAACTTCCTTGACAGAAAATGACATGTCCTCAACTAATCAGAACCAGGAGGTTGATCAAGAATGTATAAACAGTGATCACCATTATGACGTAGCTAATACGACTGAGTATCAGTGGCCATCTGAAAGCTGGGACACAGATATAGACCAGTTTCTGGGATCTGCAAAATCAAGCCTCAATGAGTTTTGTCAAAAGCGAAGCTGGGAAGTGCCTGAGTGGGTTTGCTACAAAGAGGAAGGCATGCCACACCAAAAAAGCTTTACATATATGGCTGTGTTAAAACTGCCTGAAAACATTTGTATTGAGTGCTTGGGAGAACCCAAGAAGAACAAGAGGTTGGCAATGGAATCCGCTTCACGTGGTGCACTATGGTGGCTTAATTCTCAAGGTTACTTGTAGCATATGA___CACCAAAATTTTACCATTTATATGTGCATGGATTATTGCTCCTGCCTACCAGCATCAGAAACAGCAGACTGGAGGCATTCATTATTCTTTATTTGATGACTAGATGATATTTTCCATAATGATACAAAATAGCTCCTGCTTTCTTTAAACATGAGCTATATGCGCAATAGGTTAGTTTATGACTTTCCTATGATATGTTTGTTCCTAGATATTAGAAGGAATG

Fln Alignment:

sp_v1.1_unigene3784___PDVEKTIHYFEGDNAMAEMDSLSVKCTKRHHLLHMxxxxDVVEALIGAYLVDSGFPAALAFLKWMGIEIDFDASLVGEACLQSKNNLSLIKCIDIDELERLLGYNFIHKGLLLEAFAHPSFNEHNGGCYQRLEFLGDAVLDYIITSYLYSVYPDMKPGYLTDMRSMTVNNESFASVAVRHGLYMYLIQKSDSLSSAVKKYVDFMKSLLTRDRDSYDEPEPKCPKVLGDIVESFAGALVVDTGFDLECVWKLVLRILEPIVTPETLCIQPVRELSEVCQHHNLTLDYRKVPK--GNGFLVEANVXXXXXXXXXXXXXTRTSIKDAKKVASXXXXXXXXXXX*NIHINLEVYQNMSERLCSTCR--------*EKAEKATSNIII
B8LN71______________PELVHMIHYFEGDNTMTDTDFTSVKCTKRHHWLHMxxxxDVVEALIGAYLVDSGFGAALAFLKWIGIEIDFDTSLVGEVCIRSRNNLSLIKCIDIDELERLLGYNFSYKGLLLEAFAHPSFNEHNGGCYQRLEFLGDAVLDYIITSYLYSVYPDMKPGYLTDMRSMTVNNESFACVAVRHGLYMYLIQKSDSLSSAVTKYVDFMKALFTADRDSCDEPEPKCPKVLGDIVESFAGALVIDTGFDLDCVWKLMLNILQPIVTPETLCLQPVRELSEVCQHHNLTLDYVKASKGNGNGYLVEAKVSGKKVSGSVGGTGTRTSIKDAKKVASKDALLKLKG------FGLEHPNKILEVFIRTCRKGSAVLVGKNKAEQATSNIKI

SNPs (tot: 10)

Position	A %	C %	G %	T %	Probability
156		14		85	0.993533
203	85	14			0.993533
261		14		85	0.993533
264		14		85	0.993533
265		14	85		0.993533
268		85		14	0.993533
292	83	16			0.994512
294		83		16	0.994512
1395	25		75		0.996473
1622	80		20		0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain