SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene1955

Length: 2067 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene1955
CGTCGTGACCTTGGCTGTCACTCAAGTCTGGCAAATTTTGCATTTTGATTGTTGAACCCTTCATTATCAACATCTAGGGTTACCGAGGGTTCTTCGTAGCATCTGTGTCTTAGCTCGATCGCATTCGAAAGGGATCTTCTCTGTTGGTTTTTAAATAGCTTGTGATGGCTTCTTCGAAGGATCAGAAGGCACAGGATGTCCATTCTAACGAGGATGGTGACTCAGAACGGGAGGAAAACGCCTCAGAAGAGCAAAAACAAGAAAAGAACAATGGAGTTTCCAGCCCTGGGAAAATCTTCATCGGCGGCCTGTCAAGGACTACGACGTCGAGTGCTTTTACAAAGCATTTCAGTAAGTACGGGGAGATAACGGACTCAGTTATAATGAAAGACCGCGTCAGTGGCCAGCCTCGTGGATTTGGGTTTGTGACTTATGCTGATCCCTGTGTCATAGACAAGGTTATACAGGACAAGCACGTTCTTGATGGAAAAGCGGTTGAAATCAAAAGAACCATTCCAAGGGGGAATTCGTCCAAGGGCCCAAAGACGAAAAAAGTATTTGTGGGTGGTATACCTACATCAATTACTGAAGCAATGACTTTCAGTGGTCTTCAATTTAACTTTGTGAAAGATTGACCTTTAGTGTATGTGCTGCACTAAAATAAGAGGTCAAGGTAGAAAGATATGTTAAATTGTGCAGGTAAGTAACCGAACTGTTGATGTTAATGCTTTTGATTATGGTTTTGCTTCAGTTTTTGAAACCTTCTGCCCACTTCTATTTTAGAAATTGCTCTTTTCCTGCAAGTTTCCCTTTAAAGTACAAAAATGTTAAAATGAAGGACTATACACTAACTACAGCAGGGGATTTAAGATATATTTTGTCCTCTTGAGGTATATTTTGATTGAAAATGCTGTTACTTCTCAATGAATAGTTTTTCCCCATTTTGTGCTGTTTTCTTCTATTGACTATACCAAGTGTTCTGATACATTTGGATCCTTGTTACGGACTATTCCTCATCTCGAGGGTCATCTATATGACTTTGCTCAGGGTTTGGGGATCTCCTCAAATAGTCAATCTGAAGCATATGATCCCCTTCAGGGTTATATTCCGTTTTCTTTTGATAAATGCTGTAAGAGCTAAGACATCGATTGTTATTGGTGACTTGATTATAATTATTCGTCTATTGTGCTCAAAGATGACCCCCGCTGAGAGCAAGCTAGTCATCTTTAAACACTCAGATCTAAAAGGAGATTTGCAGATTCCAGATCTGACAGGTTCTAAGAGAATAATACAATTGACAAAATGTGTATCTTGCCCTTCACCTGGACTCAGGTGAGATATTAGCAGATTGTGTGTTTGAGTGACTGTTTAGTCAATGGTGGTGGACTGCTCTGTGATGGTTTCTTCCGGGTAAGGTCATCTCTCTGTGTCCTGTCTGTGTATTTGAACTTGGACATCTGGTGCTTTGTTACTCTTATATTCTGGAAATTTGAAGTGCATATGGTGTCTAATGCCTTGGTGTTTTTGGAACTTTGGTTTCAAGGTGTTTGTTGCCTTGAAACTTTGGGTTCTGGTGAGCTTGAGTTGTTGGTGTTTGACGTTCTGGCCATCCTGCTGGTGGGGAGTGGTGATTTCAGGCGTTCTGTTGTTTGGAGATTTGGGTGGGTGGCAGTGTCAAGTAGACTAGTGCAGACTAGTTTGTATCTTGCAGCTTTGTTCTTATGTGTATCAACATTAATCAACAGGAATTATCGCTCACAATGCCAATGGTCATGAACACCTTGGAACATCAGATGCAACAAATTCATGTCCTGAGAATCCGTCAATAGTGAAAGAACATCAATACTATCCATGACCACTAACGTACTTGAAAATAAGCACCGATGTATCTCTCATAATGCAATGGACAAATGTAACAAATTCATTCCCCAGCAGTAACAGAGCCCCAATATTTTAGTAAAGTCGTTAAATGAGCACCAGTGTATCTCTCATAATGCTAATGGACATGAACAGCCTGGTGCAACTAAAGCAACATTCTTTTTTGAATATAAAGTTGCAGGTTTGTAA

Ace file of the UniGene sp_v1.1_unigene1955 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Ribonucleoprotein-like n=2 Tax=Arabidopsis RepID=Q9FM47_ARATH	-	-	6.0e-38	65%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	90%
Blast2go	rna recognition motif -containing protein	-	-	0.0	83%

Source	InterPros	Term	Type	e value	Identity
Blast2go	RNA recognition motif domain	IPR000504	-	0.0	83%
Blast2go	Nucleotide-binding, alpha-beta plait	IPR012677	-	0.0	83%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LPT8

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 255 aas, putative chimeric sequence, your sequence is shorter than subject: 157 - 411

Fln protein: MASSKDQKAQDVHSNEDGDSEREENASEEQKQEKNNGVSSPGKIFIGGLSRTTTSSAFTKHFSKYGEITDSVIMKDRVSGQPRGFGFVTYADPCVIDKVIQDKHVLDGKAVEIKRTIPRGNSSKGPKTKKVFVGGIPTSITEAMTFSGLQFNFVKD*

Protein Length: 158

Fln nts: CGTCGTGACCTTGGCTGTCACTCAAGTCTGGCAAATTTTGCATTTTGATTGTTGAACCCTTCATTATCAACATCTAGGGTTACCGAGGGTTCTTCGTAGCATCTGTGTCTTAGCTCGATCGCATTCGAAAGGGATCTTCTCTGTTGGTTTTTAAATAGCTTGTG_-_ATGGCTTCTTCGAAGGATCAGAAGGCACAGGATGTCCATTCTAACGAGGATGGTGACTCAGAACGGGAGGAAAACGCCTCAGAAGAGCAAAAACAAGAAAAGAACAATGGAGTTTCCAGCCCTGGGAAAATCTTCATCGGCGGCCTGTCAAGGACTACGACGTCGAGTGCTTTTACAAAGCATTTCAGTAAGTACGGGGAGATAACGGACTCAGTTATAATGAAAGACCGCGTCAGTGGCCAGCCTCGTGGATTTGGGTTTGTGACTTATGCTGATCCCTGTGTCATAGACAAGGTTATACAGGACAAGCACGTTCTTGATGGAAAAGCGGTTGAAATCAAAAGAACCATTCCAAGGGGGAATTCGTCCAAGGGCCCAAAGACGAAAAAAGTATTTGTGGGTGGTATACCTACATCAATTACTGAAGCAATGACTTTCAGTGGTCTTCAATTTAACTTTGTGAAAGATTGA___CCTTTAGTGTATGTGCTGCACTAAAATAAGAGGTCAAGGTAGAAAGATATGTTAAATTGTGCAGGTAAGTAACCGAACTGTTGATGTTAATGCTTTTGATTATGGTTTTGCTTCAGTTTTTGAAACCTTCTGCCCACTTCTATTTTAGAAATTGCTCTTTTCCTGCAAGTTTCCCTTTAAAGTACAAAAATGTTAAAATGAAGGACTATACACTAACTACAGCAGGGGATTTAAGATATATTTTGTCCTCTTGAGGTATATTTTGATTGAAAATGCTGTTACTTCTCAATGAATAGTTTTTCCCCATTTTGTGCTGTTTTCTTCTATTGACTATACCAAGTGTTCTGATACATTTGGATCCTTGTTACGGACTATTCCTCATCTCGAGGGTCATCTATATGACTTTGCTCAGGGTTTGGGGATCTCCTCAAATAGTCAATCTGAAGCATATGATCCCCTTCAGGGTTATATTCCGTTTTCTTTTGATAAATGCTGTAAGAGCTAAGACATCGATTGTTATTGGTGACTTGATTATAATTATTCGTCTATTGTGCTCAAAGATGACCCCCGCTGAGAGCAAGCTAGTCATCTTTAAACACTCAGATCTAAAAGGAGATTTGCAGATTCCAGATCTGACAGGTTCTAAGAGAATAATACAATTGACAAAATGTGTATCTTGCCCTTCACCTGGACTCAGGTGAGATATTAGCAGATTGTGTGTTTGAGTGACTGTTTAGTCAATGGTGGTGGACTGCTCTGTGATGGTTTCTTCCGGGTAAGGTCATCTCTCTGTGTCCTGTCTGTGTATTTGAACTTGGACATCTGGTGCTTTGTTACTCTTATATTCTGGAAATTTGAAGTGCATATGGTGTCTAATGCCTTGGTGTTTTTGGAACTTTGGTTTCAAGGTGTTTGTTGCCTTGAAACTTTGGGTTCTGGTGAGCTTGAGTTGTTGGTGTTTGACGTTCTGGCCATCCTGCTGGTGGGGAGTGGTGATTTCAGGCGTTCTGTTGTTTGGAGATTTGGGTGGGTGGCAGTGTCAAGTAGACTAGTGCAGACTAGTTTGTATCTTGCAGCTTTGTTCTTATGTGTATCAACATTAATCAACAGGAATTATCGCTCACAATGCCAATGGTCATGAACACCTTGGAACATCAGATGCAACAAATTCATGTCCTGAGAATCCGTCAATAGTGAAAGAACATCAATACTATCCATGACCACTAACGTACTTGAAAATAAGCACCGATGTATCTCTCATAATGCAATGGACAAATGTAACAAATTCATTCCCCAGCAGTAACAGAGCCCCAATATTTTAGTAAAGTCGTTAAATGAGCACCAGTGTATCTCTCATAATGCTAATGGACATGAACAGCCTGGTGCAACTAAAGCAACATTCTTTTTTGAATATAAAGTTGCAGGTTTGTAA

Fln Alignment:

sp_v1.1_unigene1955___MASSKDQKAQDVHSNEDGDSEREENASEEQKQEKNNGVSSPGKIFIGGLSRTTTSSAFTKHFSKYGEITDSVIMKDRVSGQPRGFGFVTYADPCVIDKVIQDKHVLDGKAVEIKRTIPRGNSSKGPKTKKVFVGGIPTSITE
B8LPT8______________MASSKDQKTQDVASNEDGDSEREENVEEEQKQEKNNDVSSPGKIFIGGLSRSTTSSAFTKHFSKYGELTDSVIMKDRLTGQPRGFGFVTYADPSVIDKVIQDKHILDGKTVEIKRTIPRGNSSKAPKTKKVFVGGIPTSITE

SNPs (tot: 23)

Position	A %	C %	G %	T %	Probability
582	75	25			0.996473
583	75	25			0.996473
813	80		20		0.995493
816		20		80	0.995493
946		75		25	0.996473
991		25	75		0.996473
993	25			75	0.996473
1118	66		33		0.997452
1162	20		80		0.995493
1214		25		75	0.996473
1217		25		75	0.996473
1218		25	75		0.996473
1219		25		75	0.996473
1364	25		75		0.996473
1375		20		80	0.995493
1376	20		80		0.995493
1504	66	33			0.997452
2023	91	8			0.999766
2038	22			77	1.0
2050		30		69	1.0
2051		30	69		1.0
2052	83	16			0.999991
2053	25		66		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain