SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene531

Length: 2117 nt

Origin of reads: Biogeco_1 Uma

UniGene Fasta

>sp_v1.1_unigene531
TAAGGCCAAGAGAAGAAAATTAGTAATCATTGTCTTTACGGTGTCTGCGCTGTTTATAGCTGCTGCGCTCGTGTCTGTGACAGTTGGAATCTTGGTTGTTGGGAACAAGCGCAGGACGAGATCCGGCCCAGACTCCAATGGGGCTGCCAACGCCGCAGGGGGTTGGAAATCCAAAGCAATTGATACGGCCTGCCGTAAAACACTGTACCCCCAGCTCTGCATGTCCACTCTGGTGACTTACCAGGGCGGGGAGCAGCTGCGGGAACCCAAAGATCTTGCTCACATTACACTCAATGTGACCATGGACCGAGTCCACCGGGCATACCAGGCTCTCTCACTGAACATTTCGACCCACCATGCGAAGATGCAGAACAGAGAACTGGTAGCGTTTGAAGATTGTGCGGAGCTTCTCCAAGATACCAGTTTTCATCTCAGCGCCTCCTCTGCCAAAATGCGGGCCATGTCGAAAAATCCCAAATTGATTAAAGCCCATGTTGCAGATGTGAACACTTGGCTCAGCGCAGCTCTGACAAACCAGGACACATGCCTGGATGGTTTCCAGCTTGCCGGGGTTGAAACAGTCACCAGCGCCCAGATGGGTGATCTTGATGTCAAGGCCCAAATCGAGGAAGATTCCAAAATGTTGTTGGAATTCGTCAGCAATTCTCTGGCCATGTTCCAGATCTTGTTCTGCAACACCACAAACTCTGACATTGGTGCCCTTCAGGGTCATGATTATCATGTACAGACAAACAACTTTACAGTCCCATCGTCTCCTCCAACAAAAAGGCGACGCCTTTTGGCAGAGGCAGGGGAAGAAATGAACAATGCTCTTCGGAATCAAGAATTTTATGACCATTATGGATTGATTCATGGAGGGGCGCAGCATGAATTTCCTCGGTGGTTATCTGCCCGAGATCGCAGGCTTTTGCGACTGCCCGTTGCTGCTATGCAACCTGATGCTGTTGTGGCCTTGGATGGAAGTGGCAAGTATAAGAGCATAGTTGATGCTGTCAACGATGCACCTTCCCTGCTAAGCAGCAGGAGGTATATAATCTATGTGAAAACAGGAGTGTATAACGAAAATGTCACGATTTCAAGGAAGAAGACCAATCTCATGATTGTTGGTGATGGCATTGGAAAAACTATTGTAGCAGCAGGCAAAAATGTAGCTGATGGCTCCAGTACCTTTCGTTCTGCAACTTTCGCTGCATCAGGTACAGGATTCATAGCACGCGACATGACATTCCTGAACAACGCAGGGCAGGAGAAGCACCAGGCCGTGGCTCTGAGAGTGGGAGCGGATTTTGCAGCCATTTACCGTTGCAGCGTCATCGGTTACCAGGACACACTGTACGTACATTCTCTCCGCCAATTTTACAGAGAATGCGACATCTACGGCACAGTGGACTTCATCTTTGGCAACGCGGCCGTGGTGTTGCAGAAGTGCACCATGTTCGCCAGAAAACCCCTGCCCAACTCCAAGATCACGGTGACGGCGCAGGGCAGGAAGGACCCCAACCAGAACACCGGCATCTCCATCCACGACTGCAGAGTGACGGCGGCGGCGGATCTTGCTCCCGTCAAGGGCCTCTATCGCGCTTACCTCGGGAGGCCCTGGAAATTATACTCTCGGACGGTATACCTGCAAACTTTTTTGGATGATATTATTGACCCTGCCGGATGGTTGGAGTGGTCCGGAGACTTCGCATTGGATACCTTATTTTATGGGGAATATATGAACAGTGGCCCCGGCGCAGGGCTTGCGGGGCGGGTGACGTGGCCTGGTTACAGGATTTTCAAAGCGGCGCAGGAAGTTTATCCATTCACGGTGGCCCAGTTCATCTCTGGTGCTAAATGGTTGCCATCCACTGGGATCGTTTTTATTGGAGGGCTGCTCACGTAGTTTATTTATTTTTCTGAAATTAAGGCTGACGTGTATATTATTCTGCTGTACTCTAAAATGCAATAGAATATTGCAAGCCAGGTGGCCAATAAATTAAGAGCTTTATTTTCTCTGCTCTGCAGTCAAAGTTCCATTGTATGCTATAATTTTTAATATATATTTATGTCAACTGAGATGATCTTTTCATTATACAATTAATTAACTACAAACA

Ace file of the UniGene sp_v1.1_unigene531

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Pectinesterase n=1 Tax=Picea sitchensis RepID=C0PST8_PICSI	-	-	81%
FL-Next	sp=Pectinesterase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	85%
Blast2go	pectin methylesterase	-	-	61%

Source	ECs	Term	Type	e value	Identity
Blast2go	Pectinesterase.	EC:3.1.1.11	-	0.0	61%

Source	KEGGs	Term	Identity
Blast2go	Pentose and glucuronate interconversions	00040	61%
Blast2go	Starch and sucrose metabolism	00500	61%
Blast2go	Metabolic pathways	01100	61%

Source	GOs	Term	Type	Identity
Blast2go	response to nematode	GO:0009624	Biological Process	61%
Blast2go	pectinesterase activity	GO:0030599	Molecular Function	61%
Blast2go	cytoplasm	GO:0005737	Cellular Component	61%
Blast2go	plant-type cell wall	GO:0009505	Cellular Component	61%
Blast2go	apoplast	GO:0048046	Cellular Component	61%

Source	InterPros	Term	Type	Identity
Blast2go	Pectinesterase, catalytic	IPR000070	-	61%
Blast2go	Pectinesterase inhibitor	IPR006501	-	61%
Blast2go	Pectin lyase fold/virulence factor	IPR011050	-	61%
Blast2go	Pectin lyase fold	IPR012334	-	61%
Blast2go	Pectinesterase, active site	IPR018040	-	61%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: C0PST8

Fln msg: your sequence is shorter than subject: 635 - 655

Fln protein: KAKRRKLVIIVFTVSALFIAAALVSVTVGILVVGNKRRTRSGPDSNGAANAAGGWKSKAIDTACRKTLYPQLCMSTLVTYQGGEQLREPKDLAHITLNVTMDRVHRAYQALSLNISTHHAKMQNRELVAFEDCAELLQDTSFHLSASSAKMRAMSKNPKLIKAHVADVNTWLSAALTNQDTCLDGFQLAGVETVTSAQMGDLDVKAQIEEDSKMLLEFVSNSLAMFQILFCNTTNSDIGALQGHDYHVQTNNFTVPSSPPTKRRRLLAEAGEEMNNALRNQEFYDHYGLIHGGAQHEFPRWLSARDRRLLRLPVAAMQPDAVVALDGSGKYKSIVDAVNDAPSLLSSRRYIIYVKTGVYNENVTISRKKTNLMIVGDGIGKTIVAAGKNVADGSSTFRSATFAASGTGFIARDMTFLNNAGQEKHQAVALRVGADFAAIYRCSVIGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAVVLQKCTMFARKPLPNSKITVTAQGRKDPNQNTGISIHDCRVTAAADLAPVKGLYRAYLGRPWKLYSRTVYLQTFLDDIIDPAGWLEWSGDFALDTLFYGEYMNSGPGAGLAGRVTWPGYRIFKAAQEVYPFTVAQFISGAKWLPSTGIVFIGGLLT*

Protein Length: 636

Fln nts: TAAGGCCAAGAGAAGAAAATTAGTAATCATTGTCTTTACGGTGTCTGCGCTGTTTATAGCTGCTGCGCTCGTGTCTGTGACAGTTGGAATCTTGGTTGTTGGGAACAAGCGCAGGACGAGATCCGGCCCAGACTCCAATGGGGCTGCCAACGCCGCAGGGGGTTGGAAATCCAAAGCAATTGATACGGCCTGCCGTAAAACACTGTACCCCCAGCTCTGCATGTCCACTCTGGTGACTTACCAGGGCGGGGAGCAGCTGCGGGAACCCAAAGATCTTGCTCACATTACACTCAATGTGACCATGGACCGAGTCCACCGGGCATACCAGGCTCTCTCACTGAACATTTCGACCCACCATGCGAAGATGCAGAACAGAGAACTGGTAGCGTTTGAAGATTGTGCGGAGCTTCTCCAAGATACCAGTTTTCATCTCAGCGCCTCCTCTGCCAAAATGCGGGCCATGTCGAAAAATCCCAAATTGATTAAAGCCCATGTTGCAGATGTGAACACTTGGCTCAGCGCAGCTCTGACAAACCAGGACACATGCCTGGATGGTTTCCAGCTTGCCGGGGTTGAAACAGTCACCAGCGCCCAGATGGGTGATCTTGATGTCAAGGCCCAAATCGAGGAAGATTCCAAAATGTTGTTGGAATTCGTCAGCAATTCTCTGGCCATGTTCCAGATCTTGTTCTGCAACACCACAAACTCTGACATTGGTGCCCTTCAGGGTCATGATTATCATGTACAGACAAACAACTTTACAGTCCCATCGTCTCCTCCAACAAAAAGGCGACGCCTTTTGGCAGAGGCAGGGGAAGAAATGAACAATGCTCTTCGGAATCAAGAATTTTATGACCATTATGGATTGATTCATGGAGGGGCGCAGCATGAATTTCCTCGGTGGTTATCTGCCCGAGATCGCAGGCTTTTGCGACTGCCCGTTGCTGCTATGCAACCTGATGCTGTTGTGGCCTTGGATGGAAGTGGCAAGTATAAGAGCATAGTTGATGCTGTCAACGATGCACCTTCCCTGCTAAGCAGCAGGAGGTATATAATCTATGTGAAAACAGGAGTGTATAACGAAAATGTCACGATTTCAAGGAAGAAGACCAATCTCATGATTGTTGGTGATGGCATTGGAAAAACTATTGTAGCAGCAGGCAAAAATGTAGCTGATGGCTCCAGTACCTTTCGTTCTGCAACTTTCGCTGCATCAGGTACAGGATTCATAGCACGCGACATGACATTCCTGAACAACGCAGGGCAGGAGAAGCACCAGGCCGTGGCTCTGAGAGTGGGAGCGGATTTTGCAGCCATTTACCGTTGCAGCGTCATCGGTTACCAGGACACACTGTACGTACATTCTCTCCGCCAATTTTACAGAGAATGCGACATCTACGGCACAGTGGACTTCATCTTTGGCAACGCGGCCGTGGTGTTGCAGAAGTGCACCATGTTCGCCAGAAAACCCCTGCCCAACTCCAAGATCACGGTGACGGCGCAGGGCAGGAAGGACCCCAACCAGAACACCGGCATCTCCATCCACGACTGCAGAGTGACGGCGGCGGCGGATCTTGCTCCCGTCAAGGGCCTCTATCGCGCTTACCTCGGGAGGCCCTGGAAATTATACTCTCGGACGGTATACCTGCAAACTTTTTTGGATGATATTATTGACCCTGCCGGATGGTTGGAGTGGTCCGGAGACTTCGCATTGGATACCTTATTTTATGGGGAATATATGAACAGTGGCCCCGGCGCAGGGCTTGCGGGGCGGGTGACGTGGCCTGGTTACAGGATTTTCAAAGCGGCGCAGGAAGTTTATCCATTCACGGTGGCCCAGTTCATCTCTGGTGCTAAATGGTTGCCATCCACTGGGATCGTTTTTATTGGAGGGCTGCTCACGTAG___TTTATTTATTTTTCTGAAATTAAGGCTGACGTGTATATTATTCTGCTGTACTCTAAAATGCAATAGAATATTGCAAGCCAGGTGGCCAATAAATTAAGAGCTTTATTTTCTCTGCTCTGCAGTCAAAGTTCCATTGTATGCTATAATTTTTAATATATATTTATGTCAACTGAGATGATCTTTTCATTATACAATTAATTAACTACAAACA

Fln Alignment:

sp_v1.1_unigene531___KAKRRKLVIIVFTVSALFIAAALVSVTVGILVVGNKRRTRSGPDSNGAANAAGGWKSKAIDTACRKTLYPQLCMSTLVTYQGGEQLREPKDLAHITLNVTMDRVHRAYQALSLNISTHHAKMQNRELVAFEDCAELLQDTSFHLXXXXXXXXXXXXNPKLIKAHVADVNTWLSAALTNQDTCLDGFQLAGVETVTSAQMGDLDVKAQIEEDSKMLLEFVSNSLAMFQILFCNTTNSDIGALQGHDYHVQTNNFTVPSSPPTKRRRLLAEAGEEMNNALRNQEFYDHYGLIHGGAQHEFPRWLSARDRRLLRLPVAAMQPDAVVALDGSGKYKSIVDAVNDAPSLLSSRRYIIYVKTGVYNENVTISRKKTNLMIVGDGIGKTIVAAGKNVADXXXXXXXXXXXXXXXGFIARDMTFLNNAGQEKHQAVALRVGADFAAIYRCSVIGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAVVLQKCTMFARKPLPNSKITVTAQGRKDPNQNTGISIHDCRVTAAADLAPVKGLYRAYLGRPWKLYSRTVYLQTFLDDIIDPAGWLEWSGDFALDTLFYGEYMNSGPGAGLAGRVTWPGYRIFKAAQEVYPFTVAQFISGAKWLPSTGIVFIGGLL
C0PST8_____________KAKRRKLVIIVFTLCALIIAAALVSVTVGILVVGSKRRTRSGPDSNGAANAAGGWKSKAIDTACRKTLYPQLCMSTLVTYQGGAQLREPKDLAHITLNVTMDRVQQAYQVISVNISAHDGKMGHRELVAYEDCVELLQDTIYHLSASSVKMQAMSKNPKSIKAHIADVNTWLSAALTNQDTCLEGFKLAG--DGGASQLSNFSVKAQIEEESTNLAELVSNSLAMFQILFCNTS-SDIGALEGHNYHVQTNNFTVPS-PPSNRRRLLAEGGEEMNNADLNQEFYDQYGLVQGG-QHEFPLWLSARDRRLLQLPVAAMQPDAVVAKDGSGKYKSIVDALKDAPSQLTSKRYVIYVKAGVYYENVTVSRKKTNIMIVGDGIQKTVVAAGRNVADGSSTFRSATFAASGTGFIARDMTFLNNAGQDKHQAVALRVGADFSAIYRCSIIGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAVVLQKCTMFARKPMPNEKITITAQGRKDPNQNTGISIHDCKVTAAIDLVPVKASYRAYLGRPWKLYSRTVYLQTFLDDIIDPAGWLEWYGDFALNTLYYGEYMNSGPGAGLVKRVTWPGYRVFKTADQVYPFTVAQFISGSKWLPSTGITFIGGLL

SNPs (tot: 6)

Position	A %	C %	G %	T %	Probability
1339		83		16	0.994512
1810			75	25	1.0
1921		33		66	1.0
2021		14		85	0.993533
2035	80		20		0.995493
2053	50		50		1.0

SSRs (tot: 1)

Start position	End position	Sequence	Length
1560	1571	CGG CGG CGG CGG	12

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain