SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene276

Length: 2413 nt

Origin of reads: Biogeco_1 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene276
CGTCGTGACCTTGGCTGTCACTCAATTTGTTCTAGACGACGGTGGCGAGGTTGACTTGGACCTTGGCAACTATGAAAGATTTCTGGATGTGCGATTAACTAGAGATAACAATGTCACCACTGGTAAAATATACCAGTCAGTCATTGACAAAGAAAGGAGGGGAGATTTCTTGGGAAAGACTGTCCAGGTAGTTCCACATATCACGGATGCCATTCAGGAGTGGATTGAACGCGTTTCACTTATTTCTGTGGATGGAAGTGAAAAACCTGCTGATGTATGTGTCATTGAGTTGGGGGGAACAGTAGGGGATATTGAATCAATGCCATTTATTGAGGCTTTGCGTCAGCTCTCCTATCGTGTTGGCCCAGGAAATTTCTGTCTGGTGCACTGTGAGCCTTGTACCTGTTTTAGGTGTTGTTGGTGAGCAGAAAACCAAGCCAACACAGCACAGTGTCCGTGAACTTCGAGGATTAGGTTTGACACCCAATATTCTAGCTTGTCGCAGTGCTCAGGCTTTGGAAGAGAATACTAAGGAAAAGTTATCACAATTTTGCCATGTTCCAGCCGGGAACATTCTCACTATATATGATGTTCCAAACATTTGGCATGTCCCCCTTCTACTAAGGGATCAAAAGGCTCATGAAGCAATCTTGAAAGAGTTGAACCTTTCAAGTGTTACTCGATCCCCTTGTCTGAAAAATTGGACTAAAAGAGCAGAAAATTTTGATAACCTTGTAACTCCAGTCAAAATTGCAATGGTGGGAAAATACACTGGCCTTTCTGATGCTTATCTTTCTGTTCTGAAGGCCCTTGAACATGCTTCTATAGAATGTTCGAGAAAATTGTTAGTGGAATGGGTTGCAGCATCTGACCTTGAAGACACAGTTGCAAAAGAGGCACCAGATGTTCATGCAGCTGCTTGGAAATCTCTCATGGGTGCAGATGGTGTTCTGGTTCCAGGTGGATTTGGTGATCGTGGTGTCCAAGGGAAAATACTTGCAGCAAAATATGCTCGAGAAAAGAAAATCCCATATCTTGGAATTTGCTTGGGAATGCAGATTGCTGTGATTGAGTATGCTCGTTCTGTTCTTGGATTGGTGGGGGCTAACAGCACAGAGTTTGACCCAGAAACTTCAAATCCCTGTGTTATTTTCATGCCTGAGGGTTCAAAAACCCATATGGGGGGTACCATGCGACTGGGTTCTAGGAGGACATATTTCCAGAGTGCGGATTGCATCACTGCAAAGTTATACCAAAAAGCTAAAGCTGATTATGTGGATGAGCGACATCGACATAGATATGAGGTTAACCCTGATATGGTTGAAAAGCTTGAACAGGAGGGGCTACTGTTTGTGGGCAAGGATGAAACCGATAGACGTATGCAGATACTGGAATTAACAGGCCATCCTTATTTTGTTGGAAGTCCAGTTCCATCCAGAATTCAAGTCAAGACCTGGAAAAGCATCTGCAGTCTTCTTGGGCCTCATACTAGCAGCGTCATGTCAGCTTGATTCATATTTGAACAACCCTGGCAAACAGAGCAATTTGAGTCGGAGTCCTCATGTGGATGATAGTAGCAGAAGTCCTGTATCACCTGGAGGGAATGTGTTTGAAAATGGCAGTGTAAATGGCACTGATAATGGGCTTGTGTTTGAAAATGGCAGTGTAAATGGCACTGATAATGGGCTTGTGTTTGAAAATGGCAGTGTAAATGGCACTGATAATGGGCTTGTATCAAATGGTGTTGGAAATGGCATTGTGAATGGTCCTGCTAATGGTGTTACGAAAAGGAATCCACCTCCTAACTGCTCAAATGCGAATGGAAATGGCTTCCATGCCTAAATAAGAGTGAACCGATTGAGTTTGGATTGTAAAGTTTTGGTGCTTTAAGTTTGAGTCATTGTTAGCATCATTTTTTCTTTGATACTGAAATTAAGTGTTGCTTGCTCTCAAGCATTACAAAAGAGGTCCAAATCTACAATTTGCGGAACGTTCTGTACAGAAGATAGAGTGGGGTATCTGAGATGTAGAACTTACCTGTTGGATATGACCACAGAATATATTGTTACACTATCAGGCTACAGGGTACTGGAGAACAAATTGTGTTGTGTTCTTCCTGACTTTGACAGTTCTTTGTAGTGGCTGCTGCTGATTCATTGGTGAATGCTATTTATGGTTTTAAAAAATTTCAGGTTCCCACTATGTGGGAAGCACTGTTGTCATTCAACTGATGGCTCGGGACAAAGTTTGTCATATATTTTTAATGGGTTTATACTACGCCATAAAGATATTAAATTGAAACTTGTCAATAGCAGAACTTGGAAATACATCTCTACTATGTCACAATGAAATTGTTCAAGTAACAATTGACTACTCTTATTAAGACTTGCTGCTTCCTTTTTGGAAACACATG

Ace file of the UniGene sp_v1.1_unigene276 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	CTP synthase (EC:6.3.4.2); K01937 CTP synthase [EC:6.3.4.2]	-	-	72%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	83%
Blast2go	ctp synthase	-	-	81%

Source	ECs	Term	Type	e value	Identity
AutoFact	CTP synthase.	EC:6.3.4.2	-	0.0	72%

Source	KEGGs	Term	Type	e value	Identity
AutoFact	Pyrimidine metabolism	00240		0.0	72%
AutoFact	Metabolic pathways	01100		0.0	72%

Source	GOs	Term	Type	e value	Identity
Blast2go	response to cadmium ion	GO:0046686	Biological Process	0.0	81%
Blast2go	catalytic activity	GO:0003824	Molecular Function	0.0	81%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Glutamine amidotransferase type 1	IPR017926	-	0.0	81%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LLK6

Fln msg: Separated hits, possible frame ERROR between 388 and 390, your sequence is longer than subject: 504 - 445

Fln protein: VVTLAVTQFVLDDGGEVDLDLGNYERFLDVRLTRDNNVTTGKIYQSVIDKERRGDFLGKTVQVVPHITDAIQEWIERVSLISVDGSEKPADVCVIELGGTVGDIESMPFIEALRQLSYRVGPGNFCLVxxVSLVPVLGVVGEQKTKPTQHSVRELRGLGLTPNILACRSAQALEENTKEKLSQFCHVPAGNILTIYDVPNIWHVPLLLRDQKAHEAILKELNLSSVTRSPCLKNWTKRAENFDNLVTPVKIAMVGKYTGLSDAYLSVLKALEHASIECSRKLLVEWVAASDLEDTVAKEAPDVHAAAWKSLMGADGVLVPGGFGDRGVQGKILAAKYAREKKIPYLGICLGMQIAVIEYARSVLGLVGANSTEFDPETSNPCVIFMPEGSKTHMGGTMRLGSRRTYFQSADCITAKLYQKAKADYVDERHRHRYEVNPDMVEKLEQEGLLFVGKDETDRRMQILELTGHPYFVGSPVPSRIQVKTWKSICSLLGPHTSSVMSA*

Protein Length: 505

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTTGTTCTAGACGACGGTGGCGAGGTTGACTTGGACCTTGGCAACTATGAAAGATTTCTGGATGTGCGATTAACTAGAGATAACAATGTCACCACTGGTAAAATATACCAGTCAGTCATTGACAAAGAAAGGAGGGGAGATTTCTTGGGAAAGACTGTCCAGGTAGTTCCACATATCACGGATGCCATTCAGGAGTGGATTGAACGCGTTTCACTTATTTCTGTGGATGGAAGTGAAAAACCTGCTGATGTATGTGTCATTGAGTTGGGGGGAACAGTAGGGGATATTGAATCAATGCCATTTATTGAGGCTTTGCGTCAGCTCTCCTATCGTGTTGGCCCAGGAAATTTCTGTCTGGTGCAxxxTGTGAGCCTTGTACCTGTTTTAGGTGTTGTTGGTGAGCAGAAAACCAAGCCAACACAGCACAGTGTCCGTGAACTTCGAGGATTAGGTTTGACACCCAATATTCTAGCTTGTCGCAGTGCTCAGGCTTTGGAAGAGAATACTAAGGAAAAGTTATCACAATTTTGCCATGTTCCAGCCGGGAACATTCTCACTATATATGATGTTCCAAACATTTGGCATGTCCCCCTTCTACTAAGGGATCAAAAGGCTCATGAAGCAATCTTGAAAGAGTTGAACCTTTCAAGTGTTACTCGATCCCCTTGTCTGAAAAATTGGACTAAAAGAGCAGAAAATTTTGATAACCTTGTAACTCCAGTCAAAATTGCAATGGTGGGAAAATACACTGGCCTTTCTGATGCTTATCTTTCTGTTCTGAAGGCCCTTGAACATGCTTCTATAGAATGTTCGAGAAAATTGTTAGTGGAATGGGTTGCAGCATCTGACCTTGAAGACACAGTTGCAAAAGAGGCACCAGATGTTCATGCAGCTGCTTGGAAATCTCTCATGGGTGCAGATGGTGTTCTGGTTCCAGGTGGATTTGGTGATCGTGGTGTCCAAGGGAAAATACTTGCAGCAAAATATGCTCGAGAAAAGAAAATCCCATATCTTGGAATTTGCTTGGGAATGCAGATTGCTGTGATTGAGTATGCTCGTTCTGTTCTTGGATTGGTGGGGGCTAACAGCACAGAGTTTGACCCAGAAACTTCAAATCCCTGTGTTATTTTCATGCCTGAGGGTTCAAAAACCCATATGGGGGGTACCATGCGACTGGGTTCTAGGAGGACATATTTCCAGAGTGCGGATTGCATCACTGCAAAGTTATACCAAAAAGCTAAAGCTGATTATGTGGATGAGCGACATCGACATAGATATGAGGTTAACCCTGATATGGTTGAAAAGCTTGAACAGGAGGGGCTACTGTTTGTGGGCAAGGATGAAACCGATAGACGTATGCAGATACTGGAATTAACAGGCCATCCTTATTTTGTTGGAAGTCCAGTTCCATCCAGAATTCAAGTCAAGACCTGGAAAAGCATCTGCAGTCTTCTTGGGCCTCATACTAGCAGCGTCATGTCAGCTTGATTCATATTTGAACAACCCTGGCAAACAGAGCAATTTGAGTCGGAGTCCTCATGTGGATGATAGTAGCAGAAGTCCTGTATCACCTGGAGGGAATGTGTTTGAAAATGGCAGTGTAAATGGCACTGATAATGGGCTTGTGTTTGAAAATGGCAGTGTAAATGGCACTGATAATGGGCTTGTGTTTGAAAATGGCAGTGTAAATGGCACTGATAATGGGCTTGTATCAAATGGTGTTGGAAATGGCATTGTGAATGGTCCTGCTAATGGTGTTACGAAAAGGAATCCACCTCCTAACTGCTCAAATGCGAATGGAAATGGCTTCCATGCCTAAATAAGAGTGAACCGATTGAGTTTGGATTGTAAAGTTTTGGTGCTTTAAGTTTGAGTCATTGTTAGCATCATTTTTTCTTTGATACTGAAATTAAGTGTTGCTTGCTCTCAAGCATTACAAAAGAGGTCCAAATCTACAATTTGCGGAACGTTCTGTACAGAAGATAGAGTGGGGTATCTGAGATGTAGAACTTACCTGTTGGATATGACCACAGAATATATTGTTACACTATCAGGCTACAGGGTACTGGAGAACAAATTGTGTTGTGTTCTTCCTGACTTTGACAGTTCTTTGTAGTGGCTGCTGCTGATTCATTGGTGAATGCTATTTATGGTTTTAAAAAATTTCAGGTTCCCACTATGTGGGAAGCACTGTTGTCATTCAACTGATGGCTCGGGACAAAGTTTGTCATATATTTTTAATGGGTTTATACTACGCCATAAAGATATTAAATTGAAACTTGTCAATAGCAGAACTTGGAAATACATCTCTACTATGTCACAATGAAATTGTTCAAGTAACAATTGACTACTCTTATTAAGACTTGCTGCTTCCTTTTTGGAAACACATG

Fln Alignment:

sp_v1.1_unigene276___NYERFLDVRLTRDNNVTTGKIYQSVIDKERRGDFLGKTVQVVPHITDAIQEWIERVSLISVDGSEKPADVCVIELGGTVGDIESMPFIEALRQLSYRVGPGNFCLVHxVSLVPVLGVVGEQKTKPTQHSVRELRGLGLTPNILACRSAQALEENTKEKLSQFCHVPAGNILTIYDVPNIWHVPLLLRDQKAHEAILKELNLSSVTRSPCLKNWTKRAENFDNLVTPVKIAMVGKYTGLSDAYLSVLKALEHASIECSRKLLVEWVAASDLEDTVAKEAPDVHAAAWKSLMGADGVLVPGGFGDRGVQGKILAAKYAREKKIPYLGICLGMQIAVIEYARSVLGLVGANSTEFDPETSNPCVIFMPEGSKTHMGG
B8LLK6_____________NYERFLDVKLTRNNNITTGKIYQSVIDNERRGNYLGKTVQVIPHITDAIQDWIIDVAGIPVDGKEGPADVCVIELGGTVGDIESMPFIESLRQLSYSVGPENFCLVHxVSLVPVIGVVGEQKTKPTQHSVRELRALGLTPHLLVCRSSQPLLENTKEKLSQFCHVPERNILNIYDVSNIWHVPLLLRDQKAHEAILKQLNILSISKAPDLEEWTARAVCYDNLTTPIKIAMVGKYTGLSDSYLSVLKALLHACVACSLKLSVEWVAASDLEDEAAKERPDVYASAWKVLKGAAGVLVPGGFGDRGVQGKILAAKYARENRIPYLGICLGMQIAVIEFARSVLGLEGANSAEFDPETPHPCVIFMPEIRKCFLCG

SNPs (tot: 20)

Position	A %	C %	G %	T %	Probability
1917	80		20		0.999996
1970		11	88		0.923564
1972		11		88	0.923564
1973	11		88		0.923564
1974	11			88	0.923564
1975	11		88		0.923564
2001	85			14	0.993533
2003		85	14		0.993533
2004	85			14	0.993533
2005		85		14	0.993533
2008	85		14		0.993533
2009	85		14		0.993533
2010	85			14	0.993533
2012		85	14		0.993533
2098		25		75	0.996473
2140		14		85	0.993533
2141	85	14			0.993533
2203	83	16			0.994512
2251	75		25		0.996473
2262			25	75	0.996473

SSRs (tot: 1)

Start position	End position	Sequence	Length
681	809	ACAAGCCCATTATCAGTGCCATTTACACTGCCATTTTCAAAC ACAAGCCCATTATCAGTGCCATTTACACTGCCATTTTCAAAC ACAAGCCCATTATCAGTGCCATTTACACTGCCATTTTCAAAC ACA	129

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain