SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene142267

Length: 1559 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v3.0_unigene142267 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Alpha-galactosidase n=1 Tax=Phaseolus vulgaris RepID=Q41100_PHAVU	-	-	70%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	68%
Sma3	Alpha-galactosidase	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3	Alpha-galactosidase.	EC:3.2.1.22	-	0.0	-

Source	KEGGs	Term	Identity
Sma3	Galactose metabolism	00052	%
Sma3	Glycerolipid metabolism	00561	%
Sma3	Sphingolipid metabolism	00600	%
Sma3	Glycosphingolipid biosynthesis - globo series	00603	%

Source	Gene names
Sma3	AGA1; At3g56310; At5g08370; At5g08380; CHLREDRAFT_116873; F18O21_270; F8L15_100; F8L15_110; GGT-1; GLA; GSVIVT00002617001; GSVIVT00002618001; GSVIVT00002619001; GSVIVT00011770001; GSVIVT00029420001; LOC_Os10g35070; LOC_Os10g35110; LeaGal; OJ1205_F02.7; OJ

Source	GOs	Term	Type	Identity
Sma3	vacuole	GO:0005773	Cellular Component	-
Sma3	plant-type cell wall	GO:0009505	Cellular Component	-
Sma3	apoplast	GO:0048046	Cellular Component	-
Sma3	hydrolase activity, hydrolyzing O-glycosyl compounds	GO:0004553	Molecular Function	-
Sma3	alpha-galactosidase activity	GO:0004557	Molecular Function	-
Sma3	protein binding	GO:0005515	Molecular Function	-
Sma3	transferase activity, transferring glycosyl groups	GO:0016757	Molecular Function	-
Sma3	cation binding	GO:0043169	Molecular Function	-
Sma3	carbohydrate metabolic process	GO:0005975	Biological Process	-
Sma3	metabolic process	GO:0008152	Biological Process	-
Sma3	positive regulation of flower development	GO:0009911	Biological Process	-
Sma3	leaf morphogenesis	GO:0009965	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Glycoside hydrolase, clan GH-D	IPR000111	-	-
Sma3	WW/Rsp5/WWP	IPR001202	-	-
Sma3	Glycoside hydrolase, family 27	IPR002241	-	-
Sma3	Glycoside hydrolase, family 2, N-terminal	IPR006104	-	-
Sma3	Putative Ig	IPR008009	-	-
Sma3	Glycosyl hydrolase, family 13, all-beta	IPR013780	-	-
Sma3	Aldolase-type TIM barrel	IPR013785	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G08370.1	AtAGAL2, AGAL2 alpha-galactosidase 2 chr5:2691116-2693441 REVERSE LENGTH=396	68%
RefSeq	Arabidopsis thaliana	NP_001031855.1	alpha-galactosidase 2 [Arabidopsis thaliana]	68%
RefSeq	Populus trichocarpa	XP_002331416.1	predicted protein [Populus trichocarpa]	69%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NWJ8

Fln msg: Separated hits, possible frame ERROR between 243 and 248, and overlapping frame ERROR between 1054 and 1053,

Fln protein: MEFRYGCFICAVLLALICSAVTCGAENRxxxGRRLLQNALAQTPPMGWNSWNHYGCNINENIIRETADALVSNGLSKLGYIYVNIDDCWAEPNRDQNGRLVASASKFPSGIKALADYVHSKGLKLGIYSDAGYYTCQKQPGSLGHEQVDADTFAYWGVDYLKYDNCYNDGSKPESRYPKMHDALLRTRRPIFYSLCEWGLDVPATWARQIGSSWRTTQDIEDKWESIVSRADENNEYASFAGPGGWNDPDMLEVGNGQMTAEEYGSHFSIWALMKAPLLIGCDVTSMEKKTYGILSNSxxxIAVNQDPLGVQGKKVSKQGDLEVWAGPLSNNRVAVVLWNRSNSRATITAKWEDIGLNPSAVVRVRNLWRHRELEANHQGSLTAPVKSHGCRMYVLTTVSG*

Protein Length: 403

Fln nts: ATGGGGATTACTCATCACATCGCCCAGTTTTCATAAAGCTTCAAAGGTTAAAGCTCTAACCGAATGCAGCACGCATCCTGTGGCTATAAATCTCTGTTCTCCTTACTATAGTGTACCATTGTCATTATTCATCACTACCTTCCTCGCGAGGAAACC_-_ATGGAATTTCGATATGGCTGTTTCATATGTGCCGTGCTTTTGGCCTTGATATGTTCTGCGGTGACCTGTGGTGCTGAGAATAGAACxxxxxxAGGACGGCGTTTGCTTCAGAATGCCCTTGCGCAGACCCCTCCTATGGGGTGGAATAGCTGGAATCACTACGGCTGCAACATCAATGAGAATATTATACGAGAAACAGCTGATGCGTTGGTATCAAATGGTCTCTCTAAGTTGGGGTATATCTACGTAAATATAGATGATTGCTGGGCAGAGCCTAATCGTGATCAAAATGGGAGATTGGTTGCAAGTGCTTCAAAGTTTCCCTCTGGTATCAAGGCTCTTGCTGATTATGTGCACAGCAAGGGTCTCAAACTGGGAATATATTCAGATGCTGGGTACTATACCTGTCAAAAGCAGCCAGGTTCCTTAGGACACGAGCAAGTAGATGCTGATACGTTTGCTTATTGGGGTGTGGATTATCTCAAATATGATAACTGTTACAATGATGGCTCTAAGCCTGAAAGCCGGTACCCAAAGATGCATGATGCACTTTTGAGAACTCGGCGGCCAATATTTTATTCCCTGTGTGAATGGGGATTGGATGTTCCAGCCACATGGGCTCGGCAAATTGGAAGCAGTTGGAGGACAACACAAGACATTGAGGACAAGTGGGAAAGCATTGTATCCCGTGCAGATGAAAACAATGAATATGCAAGCTTTGCTGGTCCAGGTGGATGGAATGACCCAGATATGTTGGAAGTCGGAAATGGCCAAATGACTGCTGAGGAATATGGTTCACATTTTAGCATCTGGGCTCTGATGAAGGCACCCTTATTGATTGGATGTGATGTAACATCTATGGAAAAGAAGACTTATGGAATCCTAAGTAACTCTGxxxxxxTAATTGCAGTCAATCAAGATCCTTTGGGAGTACAAGGGAAAAAAGTGAGCAAGCAAGGGGACCTCGAGGTATGGGCAGGTCCTCTATCTAACAACCGTGTAGCTGTGGTTCTATGGAATAGAAGCAACTCAAGAGCTACAATCACTGCCAAATGGGAAGATATTGGATTGAATCCCTCAGCTGTTGTGAGAGTTAGAAACTTATGGAGGCACAGAGAATTGGAAGCAAATCACCAAGGCAGTCTTACAGCACCAGTGAAGTCTCATGGCTGTAGGATGTACGTTTTAACCACCGTTTCTGGCTAG___TGACCATCTAAACAAATGATTGTGAAAGTTCCAGATTCTTTTTTCCTATATGTATAGATTTGGCTAGTCTAGAATTGGTGGGTGATGCTACTCAAAAAAGAGACATCACACTCCATTTTCTTAATTAGGAATATTTACATTATTTTTCTCTTTTTCGACATCTTGTTGAAAGAGAGCAAGTTAGGAAATCGTCATACTTGT

Fln Alignment:

AllPine_a_rep_c106323___MEFRYGCFICAVLLALICSAVTCGAENRTxxGRRLLQNALAQTPPMGWNSWNHYGCNINENIIRETADALVSNGLSKLGYIYVNIDDCWAEPNRDQNGRLVASASKFPSGIKALADYVHSKGLKLGIYSDAGYYTCQKQPGSLGHEQVDADTFAYWGVDYLKYDNCYNDGSKPESRYPKMHDALLRTRRPIFYSLCEWGLDVPATWARQIGSSWRTTQDIEDKWESIVSRADENNEYASFAGPGGWNDPDMLEVGNGQMTAEEYGSHFSIWALMKAPLLIGCDVTSMEKKTYGILSNSExIAVNQDPLGVQGKKVSKQGDLEVWAGPLSNNRVAVVLWNRSNSRATITAKWEDIGLNPSAVVRVRNLWRHRELEANHQGSLTAPVKSHGCRMYVLTTVS
A9NWJ8________________MDFQYGCFVHAVLLAFACCAVTCGAQNTTxxGRSLLQSGLAQTPPLGWNSWNHFGCHINEDVIRETADALVSTGLSKLGYQYVNIDDCWAELNRDSNGRLVASASRFPSGIKALADYVHSKGLKLGIYSDAGYYTCQKQPGSLGHEQVDADTFAYWGVDYLKYDNCYNDGSKPENRYPKMRDALLSTRRPIFYSLCEWGLDVPATWARQIGSSWRTTDDIEDKWESMISRADQNNEFAQYAGPGGWNDPDMLEVGNGNMTPEEYGSHFSIWALMKAPLLIGCDVTSMDKKTYGILSNSExIAVNQDPLGVQGKKVNKLGDLEVWAGPLSNNRVAVVLLNRSNSRTIITAKWEDIGLNPSAVVRVRNLWRHRELEANHQGSLTASVKPHGCKMYVLTSVS

SNPs (tot: 2)

Position	A %	C %	G %	T %	Probability
696			83	16	0.994512
790		75	25		0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain