SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene127163

Length: 1027 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v3.0_unigene127163 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Cinnamic acid 4-hydroxylase n=1 Tax=Ginkgo biloba RepID=Q3L2Q3_GINBI	-	-	77%
FL-Next	tr=Trans-cinnamate 4-hydroxylase; Pinus taeda (Loblolly pine).	-	-	73%
Sma3	Cinnamic acid 4-hydroxylase	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3	Trans-cinnamate 4-monooxygenase.	EC:1.14.13.11	-	0.0	-

Source	KEGGs	Term	Identity
Sma3	Phenylalanine metabolism	00360	%
Sma3	Phenylpropanoid biosynthesis	00940	%
Sma3	Flavonoid biosynthesis	00941	%
Sma3	Stilbenoid, diarylheptanoid and gingerol biosynthesis	00945	%
Sma3	Biosynthesis of phenylpropanoids	01061	%
Sma3	Biosynthesis of alkaloids derived from shikimate pathway	01063	%
Sma3	Metabolic pathways	01100	%
Sma3	Biosynthesis of secondary metabolites	01110	%

Source	Gene names
Sma3	BcCyp450; C4H; C4H-1; C4H-2; C4H-BN-6; C4H-BN-7; C4H-BO-1; C4H-BO-2; C4H-BO-3; C4H-BO-4; C4H-BR-1; C4H-BR-2; C4H-BR-3; C4H1; C4H1-1; C4H1-2; C4H2; C4H2-1; C4H2-2; C4H2\|CYP73A42; C4H3; CYP73; CYP73A1; CYP73A10; CYP73A11; CYP73A12; CYP73A13; CYP73A14; CYP73

Source	GOs	Term	Type	Identity
Sma3	endoplasmic reticulum	GO:0005783	Cellular Component	-
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	plant-type cell wall	GO:0009505	Cellular Component	-
Sma3	monooxygenase activity	GO:0004497	Molecular Function	-
Sma3	iron ion binding	GO:0005506	Molecular Function	-
Sma3	electron carrier activity	GO:0009055	Molecular Function	-
Sma3	trans-cinnamate 4-monooxygenase activity	GO:0016710	Molecular Function	-
Sma3	heme binding	GO:0020037	Molecular Function	-
Sma3	oxidation-reduction process	GO:0055114	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Cytochrome P450	IPR001128	-	-
Sma3	Cytochrome P450, E-class, group I	IPR002401	-	-
Sma3	Aldehyde dehydrogenase, conserved site	IPR016160	-	-
Sma3	Cytochrome P450, conserved site	IPR017972	-	-
Sma3		IPR017973	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT2G30490.1	ATC4H, C4H, CYP73A5, REF3 cinnamate-4-hydroxylase chr2:12993861-12995683 REVERSE LENGTH=505	69%
RefSeq	Arabidopsis thaliana	NP_180607.1	trans-cinnamate 4-monooxygenase [Arabidopsis thaliana]	69%
RefSeq	Populus trichocarpa	XP_002319975.1	cytochrome P450 cinnamate 4-hydroxylase [Populus trichocarpa]	73%

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: Q9XEH8

Fln msg: Separated hits, possible frame ERROR between 849 and 851, Distance to subject end: 98 aas, your sequence is shorter than subject: 343 - 506

Fln protein: ALPIFGNWLQVGMI*ITETLAI*PRNMAKSFCSRWARGTLW*FHLPIWPRRSCTPRGEFGSRTRNVVFDIFTGKGQDMVFTVYGDHWRKMRRIMTVPFFTNKVVQHYRFAWEDEISRVVADVKSRAESSTSGIVIRRRLQLMMYNIMYRMMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLRICTEIKEKRLSLFKDYFVEERKKLASTKTSINSGELKCAMDHILDAQSKGEINEDNVLYIVENINVAAIETTLWSMEWGIAxxTEPDTTRLPYLQAVVKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLAN

Protein Length: 344

Fln nts: GCATTGCCCATATTTGGGAACTGGTTGCAGGTCGGGATGATTTGAATCACAGAAACCTTAGCGATTTAGCCAAGAAATATGGCAAAATCTTTCTGCTCAAGATGGGCCAGAGGAACCTTGTGGTAGTTTCATCTCCCGATCTGGCCAAGGAGGTCCTGCACACCCAGGGGGGAGTTCGGGTCTCGAACCCGGAACGTGGTGTTCGATATCTTCACCGGCAAGGGGCAGGACATGGTGTTCACCGTCTATGGAGATCACTGGAGAAAGATGCGTAGGATCATGACTGTGCCTTTCTTTACGAATAAAGTTGTCCAGCACTACAGATTCGCGTGGGAAGACGAGATCAGCCGCGTGGTCGCGGATGTGAAATCCCGCGCCGAGTCTTCCACCTCGGGCATTGTCATCCGCAGGCGCCTCCAGCTCATGATGTATAATATTATGTACAGGATGATGTTCGACAGGAGATTCGAATCCGAGGACGACCCGCTTTTCCTCAAGCTCAAGGCCCTCAACGGAGAGCGAAGCCGATTGGCCCAGAGCTTTGAGTACAATTATGGGGATTTCATTCCCATTCTCAGGCCCTTCCTCAGAGGTTATCTCAGAATCTGCACTGAGATTAAAGAGAAACGGCTCTCTCTTTTCAAGGACTACTTCGTCGAAGAGCGCAAGAAGCTCGCCAGTACCAAAACTAGTATCAACTCCGGGGAGCTCAAGTGTGCAATGGACCATATATTAGATGCTCAGAGCAAGGGAGAGATCAATGAGGATAATGTTTTGTACATCGTTGAGAACATCAACGTTGCAGCAATTGAGACAACGCTGTGGTCGATGGAATGGGGAATAGCAGAxxxAACGGAACCAGACACGACAAGGTTGCCCTACCTTCAGGCGGTTGTGAAGGAAACCCTTCGTCTCCGCATGGCGATCCCGTTGCTCGTCCCCCACATGAATCTCCACGACGCCAAGCTCGGGGGCTACGATATTCCGGCAGAGAGCAAGATCTTGGTGAACGCCTGGTGGTTGGCCAAC

Fln Alignment:

AllPine_a_rep_c68942___ALPIFGNWLQVGMI*ITETLAI*PRNMAKSFCSRWARGTLW*FHLPIWPRRSCTPRG-EFGSRTRNVVFDIFTGKGQDMVFTVYGDHWRKMRRIMTVPFFTNKVVQHYRFAWEDEISRVVADVKSRAESSTSGIVIRRRLQLMMYNIMYRMMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLRICTEIKEKRLSLFKDYFVEERKKLASTKTSINSGELKCAMDHILDAQSKGEINEDNVLYIVENINVAAIETTLWSMEWGIAExTEPDTTRLPYLQAVVKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLAN
Q9XEH8________________AVPIFGNWLQVGDDLNHRNLGDLAKKYGEIFLLKMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQYRFAWEDELGRAVDDIKKRPEASTTGIVIRRRLQLVMYNIMYRMMFDRRFESEDDPLFLRLKALNGERSRLAQSFEYNYGDFIPVLRPFLRGYLKICKEVKESRLLLFKDYFIDERKKLASTTGSKVTGD-KCAIDHIFEAEEKGEINEDNVLYIVENINVAAIETTLWSMEWGLAExTEPDTTKLPYLQAVVKETLRLHMAIPLLVPHMNLHQAKLGGYDIPAESKILVNAWFLAN

SNPs (tot: 2)

Position	A %	C %	G %	T %	Probability
767		14		85	0.993533
857	33		66		0.997459

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain