SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene26995

Length: 2076 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v3.0_unigene26995
GAAGGCTAGATCCAGTGCTGAGAATCCTGGATGATTCAATCGCGAGCTTCAGAGGTCATCGTCAAAGTTGTTCTCAAGAACCGACTGTGTATGACGACGATGAGCCTTTGGATTCGAACTTGCGACCTAATGAAGGGCAAACAAGCTTACTAACCGATGATGATCGGGAGTTCGAATTTAAGGGTTTGTTCGTCGACCACGAAGGAACAAGCGATCGAGCTGGCGGAAGTGGAAGGAAAATGAGAATAGCATTGCATCCCGAGGTCGAAGCCGTTGCGGCGGGTGAAGCCTGCGACAATTTCACAGTGCTGGTACATGTCAAGGCTCCATCAGCGGGCGAAGATTCCAAAAAACAAAATTATGAAGACCGCGAGGGAAATATGGTGAAAGATCCAGGCTGCCGTGCGCCAATCGATCTTGTTACGGTGCTCGACGTTAGCGGAAGCATGTCGGGAATGAAGTTGGCGCTCTTGAAACGGGCCATGGCTTTTGTCATATCAAATCTGAGCCCAATGGACCGGTTATCCGTCGTTGTATTCTCTTCGACGGCAAAGCGAGTTTTTCCCTGAAGCGCATGTCTTCAGACGGCCAGCTAGAAGCGAACCTCCTAGTCGAGAGACTGTTCTGCACGGGGGGTACGAATATCGCCGAGGGTTTGAAGAAAGGCGCGAAGGTGCTCGAAGATCGTCGCGAACGGAATCCTGTGGCGAGCATCATGTTGCTCTCGGATGGCCAGGACACTTACAGTCTCAGTTCTCGCGGGACTATTTTGTTTCCCGGCGATGAAAGGAGCGCCAGACAAAGCATACGTCATGGCCACGCTCAAATTCCCGTCCATGCCTTCGGCTTCGGAGTCGACCACGATGCAGCCACGATGCACAACATTTCTGAAGAGTCCGGGGGCACGTTTTCATTCATTCAGACCGAAAGTCTCGTGCAAGACGCCTTCGCTCAGTGTATTGGTGGCCTGCTCAGCGTCGTTGTGCAGGACGTCAGGATAACAGTTTCGGCTTGCGAAGGAACGAAACTGAAAAGCTTCCATGCCGGAAGCTACGAAACCTGTGTTGCTGAGGACGGAAGCCACGGGACGGTGGAACTCGGTGATCTCTACGCCGAAGAAGATAGATATATTCTAGTGGATCTCAAATTGCCAGCCGTAAAATCTGCATCAAACCTTGTGAATTTAATCTCGGTCCGTTGCTTCCATAAAGAACCTGTTTCTCAGCAAGATTTCAATTCACGAGAACAGTATCTTTCAATTTTGCGTCCGGAGAGCACGGACGGAGCTCCGGTTGCTCTAAATTTGCAAGTGGAGAAGCAAAGGATCCGTCTCAGAACGACTCAAGCCATAGCTGAAGCCAGAGCGCTTGCCGATCAGGGCGAAATGTCGTCTGCACAGAGGGTTCTCCAGAATGCCAAAATGGAATTGAAACAGATTCGAACCGGCCACCACTCCCTTTCCCTTGCACTGGAAGCGGAAATTACAGAAATTCTAGCGAGGATGATGAGCAAGCAAACATACGAGCGGTCCGGGCGGGCGTTTGTACTTTCTGCTCAGAGCTCCCATTTTCGACAGAGAGCCACCACACGCGGCGAAAGCTTTGAGAATTACAGCACGGAATACCAGACGCCATCCATGGCGGATATGGTTATGCGTTCCCAAACTTTCTCGGCAACGGCATCATTTAACTCTACTTCCAATTCTACCGCGAGTTCGAGTTCGAGTTCAATCAATGCTCGGTGAACGTCTCAGAAACTCGCTTAAATCGATATACAGAGGAAATCGTGTGCCAGGTTAACAATCCGAATGGTGGATTCTGAGGTATAAAATTTAAATGATACTTGTGATGACATTTCTTTACAAGTGCCAATTAAATTTTTGAAGACCTAAAACAGTCAAATAAGCAATAATATCGTAGAAAGCGTTTTTCATTGCTCTTCCCATCTGAAAATCAGAGACCCTAAACGTTTAGTTTCTCATCAAACTGTAGAAAAATTGAATTTGTTTTTCATTTTTGAAAAGCTGTTGATAGAAATTTAATTTTATGCTTTTCTTTGAGCTTGTGTGATGAAATG

Ace file of the UniGene sp_v3.0_unigene26995 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Protein binding protein, putative n=1 Tax=Ricinus communis RepID=B9T7C4_RICCO	-	-	0.0	49%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	94%

Source	Gene names
Sma3	At1g08050; At2g38970; At3g54780; At5g60710; B1099H05.5; GSVIVT00018177001; GSVIVT00024560001; GSVIVT00035545001; LOC_Os03g04890; LOC_Os10g32740; LOC_Os11g11610; OJ1111_C07.20; OJ1212_C01.33; OJ1372_D06.16; OSIGBa0101P20.12; OSJNBa0043L24.22; OSJNBa0049O12

Source	GOs	Term	Type	Identity
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	protein serine/threonine kinase activity	GO:0004674	Molecular Function	-
Sma3	protein binding	GO:0005515	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	zinc ion binding	GO:0008270	Molecular Function	-
Sma3	protein phosphorylation	GO:0006468	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	IQ motif, EF-hand binding site	IPR000048	-	-
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Zinc finger, RING-type	IPR001841	-	-
Sma3	von Willebrand factor, type A	IPR002035	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-
Sma3	Zinc finger, C3HC4 RING-type	IPR018957	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G60710.1	Zinc finger (C3HC4-type RING finger) family protein chr5:24410953-24414849 REVERSE LENGTH=704	47%
RefSeq	Arabidopsis thaliana	NP_200879.1	C3H4 type zinc finger protein [Arabidopsis thaliana]	47%
RefSeq	Populus trichocarpa	XP_002316375.1	predicted protein [Populus trichocarpa]	47%

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: C0PS55

Fln msg: Overlapping hits, possible frame ERROR between 563 and 551, query STOP codon is far from subject stop. Distance to subject end: 23 aas, your sequence is shorter than subject: 588 - 829

Fln protein: RLDPVLRILDDSIASFRGHRQSCSQEPTVYDDDEPLDSNLRPNEGQTSLLTDDDREFEFKGLFVDHEGTSDRAGGSGRKMRIALHPEVEAVAAGEACDNFTVLVHVKAPSAGEDSKKQNYEDREGNMVKDPGCRAPIDLVTVLDVSGSMSGMKLALLKRAMAFVISNLSPMDRLSVVVFSSTxxxxxxLKRMSSDGQLEANLLVERLFCTGGTNIAEGLKKGAKVLEDRRERNPVASIMLLSDGQDTYSLSSRGTILFPGDERSARQSIRHGHAQIPVHAFGFGVDHDAATMHNISEESGGTFSFIQTESLVQDAFAQCIGGLLSVVVQDVRITVSACEGTKLKSFHAGSYETCVAEDGSHGTVELGDLYAEEDRYILVDLKLPAVKSASNLVNLISVRCFHKEPVSQQDFNSREQYLSILRPESTDGAPVALNLQVEKQRIRLRTTQAIAEARALADQGEMSSAQRVLQNAKMELKQIRTGHHSLSLALEAEITEILARMMSKQTYERSGRAFVLSAQSSHFRQRATTRGESFENYSTEYQTPSMADMVMRSQTFSATASFNSTSNSTASSSSSSINAR*TSQKLA*

Protein Length: 589

Fln nts: GAAGGCTAGATCCAGTGCTGAGAATCCTGGATGATTCAATCGCGAGCTTCAGAGGTCATCGTCAAAGTTGTTCTCAAGAACCGACTGTGTATGACGACGATGAGCCTTTGGATTCGAACTTGCGACCTAATGAAGGGCAAACAAGCTTACTAACCGATGATGATCGGGAGTTCGAATTTAAGGGTTTGTTCGTCGACCACGAAGGAACAAGCGATCGAGCTGGCGGAAGTGGAAGGAAAATGAGAATAGCATTGCATCCCGAGGTCGAAGCCGTTGCGGCGGGTGAAGCCTGCGACAATTTCACAGTGCTGGTACATGTCAAGGCTCCATCAGCGGGCGAAGATTCCAAAAAACAAAATTATGAAGACCGCGAGGGAAATATGGTGAAAGATCCAGGCTGCCGTGCGCCAATCGATCTTGTTACGGTGCTCGACGTTAGCGGAAGCATGTCGGGAATGAAGTTGGCGCTCTTGAAACGGGCCATGGCTTTTGTCATATCAAATCTGAGCCCAATGGACCGGTTATCCGTCGTTGTATTCTCTTCGACGxxxxxxxxxxxxxxxxxxCTGAAGCGCATGTCTTCAGACGGCCAGCTAGAAGCGAACCTCCTAGTCGAGAGACTGTTCTGCACGGGGGGTACGAATATCGCCGAGGGTTTGAAGAAAGGCGCGAAGGTGCTCGAAGATCGTCGCGAACGGAATCCTGTGGCGAGCATCATGTTGCTCTCGGATGGCCAGGACACTTACAGTCTCAGTTCTCGCGGGACTATTTTGTTTCCCGGCGATGAAAGGAGCGCCAGACAAAGCATACGTCATGGCCACGCTCAAATTCCCGTCCATGCCTTCGGCTTCGGAGTCGACCACGATGCAGCCACGATGCACAACATTTCTGAAGAGTCCGGGGGCACGTTTTCATTCATTCAGACCGAAAGTCTCGTGCAAGACGCCTTCGCTCAGTGTATTGGTGGCCTGCTCAGCGTCGTTGTGCAGGACGTCAGGATAACAGTTTCGGCTTGCGAAGGAACGAAACTGAAAAGCTTCCATGCCGGAAGCTACGAAACCTGTGTTGCTGAGGACGGAAGCCACGGGACGGTGGAACTCGGTGATCTCTACGCCGAAGAAGATAGATATATTCTAGTGGATCTCAAATTGCCAGCCGTAAAATCTGCATCAAACCTTGTGAATTTAATCTCGGTCCGTTGCTTCCATAAAGAACCTGTTTCTCAGCAAGATTTCAATTCACGAGAACAGTATCTTTCAATTTTGCGTCCGGAGAGCACGGACGGAGCTCCGGTTGCTCTAAATTTGCAAGTGGAGAAGCAAAGGATCCGTCTCAGAACGACTCAAGCCATAGCTGAAGCCAGAGCGCTTGCCGATCAGGGCGAAATGTCGTCTGCACAGAGGGTTCTCCAGAATGCCAAAATGGAATTGAAACAGATTCGAACCGGCCACCACTCCCTTTCCCTTGCACTGGAAGCGGAAATTACAGAAATTCTAGCGAGGATGATGAGCAAGCAAACATACGAGCGGTCCGGGCGGGCGTTTGTACTTTCTGCTCAGAGCTCCCATTTTCGACAGAGAGCCACCACACGCGGCGAAAGCTTTGAGAATTACAGCACGGAATACCAGACGCCATCCATGGCGGATATGGTTATGCGTTCCCAAACTTTCTCGGCAACGGCATCATTTAACTCTACTTCCAATTCTACCGCGAGTTCGAGTTCGAGTTCAATCAATGCTCGGTGAACGTCTCAGAAACTCGCTTAAATCGATATACAGAGGAAATCGTGTGCCAGGTTAACAATCCGAATGGTGGATTCTGAGGTATAAAATTTAAATGATACTTGTGATGACATTTCTTTACAAGTGCCAATTAAATTTTTGAAGACCTAAAACAGTCAAATAAGCAATAATATCGTAGAAAGCGTTTTTCATTGCTCTTCCCATCTGAAAATCAGAGACCCTAAACGTTTAGTTTCTCATCAAACTGTAGAAAAATTGAATTTGTTTTTCATTTTTGAAAAGCTGTTGATAGAAATTTAATTTTATGCTTTTCTTTGAGCTTGTGTGATGAAATG

Fln Alignment:

Contig26995___RLDPVLRILDDSIASFRGHRQSCSQEPTVYDDDEPLDSNLRPNEGQTSLLTDDDREFEFKGLFVDHEGTSDRAGGSGRKMRIALHPEVEAVAAGEACDNFTVLVHVKAPSAGEDSKKQNYEDREGNMVKDPGCRAPIDLVTVLDVSGSMSGMKLALLKRAMAFVISNLSPMDRLSVVVFSSTAxxxxSLKRMSSDGQLEANLLVERLFCTGGTNIAEGLKKGAKVLEDRRERNPVASIMLLSDGQDTYSLSSRGTILFPGDE--RSARQSIRHGHAQIPVHAFGFGVDHDAATMHNISEESGGTFSFIQTESLVQDAFAQCIGGLLSVVVQDVRITVSACEGTKLKSFHAGSYETCVAEDGSHGTVELGDLYAEEDRYILVDLKLPAVKSASNLVNLISVRCFHKEPVSQQDFNSREQYLSILRPESTDGAPVALNLQVEKQRIRLRTTQAIAEARALADQGEMSSAQRVLQNAKMELKQIRTGHHSLSLALEAEITEILARMMSKQTYERSGRAFVLSAQSSHFRQRATTRGESFENYSTEYQTPSMADMVMRSQXXXXXXXXXXXXXXXXXXXXXSINAR*TSQKLA*IDIQRKSCARLT
C0PS55_______________RLDPVLRILDDSIASFRGHRQSCSQEPTVYDDDEPLDSNLRPNEGQTSLLTDDDREFEFKGLFVDHEGTSDRAAGNARKMRIALYPEVEAVAAGEACENFTVLVHVKAPSASEASKKQNYEDCEGNMVKDPGCRAPIDLVTVLDVSGSMSGTKLALLKRAMAFVISNLSPEDRLSVVVFSSTAxxxxSLKRMTPDGQRAANRVVERLLCTGGTNIAEGLRKGAKVLEDRRQRNPVASIMLLSDGQDTYSLSSRGVVLFPSDEQRRSARQSTRYGHVQIPVHAFGFGVDHDAATMHAISEVSGGTFSFIQAESLVQDAFAQCIGGLLSVVVQDVRVTVSACAGTKLKSFHAGSYETCVAEDGSHGTVNLGDLYAEEERDILVELKFPAVKSASNPMNLISVGCFFKDPVSQRSFHSREQSFSILRPESTDGLPVALNLEVEKERNRLRTAQAIAEARTLADQGDMSGAQRVLQNAKIELQQTRTGDHSLSLALEAEITEIQARMTNIQTYERSGRAFVLSAQSSHFRQRATTRGESFESYSREYQTPSMADMVMRSQ----TLSATASSNPASISTATSISARRTSEKLAKIAMLRKSCARVS

SNPs (tot: 13)

Position	A %	C %	G %	T %	Probability
456		12		87	0.992554
558			14	85	0.993533
601	20		80		0.995493
784	66		33		1.0
1349		75	25		0.996473
1350	25	75			0.996473
1351	25		75		0.996473
1352	75	25			0.996473
1353			75	25	0.996473
1358	75			25	0.996473
1360	75		25		0.996473
1362		25	75		0.996473
1363	75		25		0.996473

SSRs (tot: 1)

Start position	End position	Sequence	Length
349	367	GAACTC GAACTC GAACTC G	19

Back

Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain