SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene25105

Length: 1755 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene25105

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	NADH-ubiquinone oxidoreductase chain 2 n=34 Tax=Magnoliophyta RepID=NU2M_ARATH	-	-	0.0	79%
FL-Next	sp=NAD(P)H-quinone oxidoreductase subunit 2, chloroplastic; Pinus thunbergii (Japanese black pine) (Pinus thunbergiana). Plastid; Chloroplast.	-	-	0.0	25%
Sma3	NADH dehydrogenase subunit 2	-	-	2.8026e-45	-

Source	ECs	Term	Type	e value	Identity
Sma3	NADH dehydrogenase (ubiquinone).	EC:1.6.5.3	-	7.116e-11	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Oxidative phosphorylation	00190		7.116e-11	%
Sma3	Metabolic pathways	01100		7.116e-11	%

Source	Gene names
Sma3	AtMg00285; AtMg00285/AtMg01320; AtMg01320; GSVIVT00035633001; HAM_042; MicpuN_mit23; NAD2; ND2; OtMtg00340; RCOM_2018870; RCOM_2136790; VITISV_023797; nad2; nd2; orf197; orf197b;

Source	GOs	Term	Type	Identity
Sma3	mitochondrion	GO:0005739	Cellular Component	-
Sma3	mitochondrial inner membrane	GO:0005743	Cellular Component	-
Sma3	integral to membrane	GO:0016021	Cellular Component	-
Sma3	NADH dehydrogenase (ubiquinone) activity	GO:0008137	Molecular Function	-
Sma3	transport	GO:0006810	Biological Process	-
Sma3	ATP synthesis coupled electron transport	GO:0042773	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	NADH:ubiquinone/plastoquinone oxidoreductase	IPR001750	-	-
Sma3		IPR003916	-	-
Sma3	Phosphopantetheine attachment site	IPR006162	-	-
Sma3	NAD(P)H-quinone oxidoreductase, subunit N/subunit 2	IPR010096	-	-

Source	Species	ID	Description	e value	Identity
ATG	Arabidoptis thaliana	ATMG01320.1	NAD2B, NAD2.2, NAD2 NADH dehydrogenase 2B chrM:327890-333105 REVERSE LENGTH=499	0.0	79%
RefSeq	Arabidopsis thaliana	NP_085584.1	NADH dehydrogenase subunit 2 (mitochondrion) [Arabidopsis thaliana]	0.0	79%

Full-Lengther Next Prediction

Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: B1N7Z3

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 260 aas, W1: There is no M at the beginning, your sequence is shorter than subject: 231 - 492

Fln protein: KISPNPFLFLLPHVQSFLAVFPEIFLINATLILLIHGVVFSTSKKYDYPPLASNVGWLGLLSVLITMLLLAAGAPLLALLFVEQGNNLTIAHFFWNHFLRKDDFTYFCQIFLLLSTAGTILMCFDYFEQERFNAFEFIVLILLSTCSMLFMISAYDLIAMYLAIELQSLCFYVIAASKRNSEFSTEAGLKYLILGAFSSGILLFGYDWTTTDICYFRQHYGLYYGYMLFL*

Protein Length: 232

Fln nts: ATATAGATCCATACATAGATACGGATATGGATCTCCATCTTACTTACGCGACTTAATACTGGCTGGCCTGGTGGGGGGCTTAAAATGCCCACGGTAAACTATATGGGAATTGGGTACGGGTATGTACAGAGCGTGGAGCCATCCTGCCTGATGGGTGCGCGCTTTCAATACGTTACGCCTTCTCTTCAACGCGTTCTTGGTTGGGATGAAACTGACCCTGCCCACGAGGGGGAGATCCGACTGACCCTACTAAGAAAGAGGAGACTCAGGATGCGGGGCCTGCGGCAGAGCCACAACACGGGAAAACGAACAACGAGATCATTGATAGGTATAACTGGCTCGTGGCCTGTTGAACGACTACAGCTAGCTGTAGCGAGAACCTGAGATCGCTGATTAAGGGGCGATCTATAAGGGGCTGCTATGTACACTTGGGTGGGCCATAAGAGCCCCCATTGTCAAGATGTATGTATGGAAAGGACCTGAGCGCTTATCCTGGCTCGCTCCGGCCGGTACCTATAGATATAGGGGGGATAACGAGATGGATCGGATTCCTAAGGGATCATTGACCATGGGACCAGCCGAATCGACGTCCCTGCCTCCCGGAGAACAGCTGTTGTCAACGAAGCGACCTGTACATTCCGTGAAAATGAGAAAGGATTATGGGGGACCTGGTCCTCTGGTCCTGCAGGCAGAAGGAAGGCCGCGAGAGGAAGGAGTGGATACTAGGAAACATATCCCATTATGTAAGGGGACAAAGGTTTCCATCCGTGCAAAACCAGCAAATAGCGTATATCTTATCTAAGAGTATAGTAGTTTCCCAAACCGATAGGGATAACGGGAAAGGAAGAAGGAGGAAGAACCGCGAGGTTATCCCTTCCCCTACCCCCCGACTAGTATAACCTTGCGGAATCACCCGAACCCAGGCCTTCCTACAGTAACTTAGTGCTGCGCCGCGCTAATTTAGCCCAAGCCGGAG_-_ATGAAAAATATCTCCGAATCCCTTCCTATTCCTTCTTCCACATGTTCAATCTTTTTTAGCGGTTTTCCCAGAGATCTTTCTCATTAACGCCACTTTAATTCTGCTCATTCATGGAGTTGTATTTAGTACCTCTAAGAAATATGATTATCCACCGTTAGCAAGTAATGTGGGTTGGCTTGGATTACTCAGTGTTCTAATAACCATGCTTTTGCTCGCCGCTGGCGCACCTCTCCTTGCCCTCCTATTTGTCGAGCAAGGGAATAACCTAACTATCGCCCATTTTTTCTGGAATCATTTTTTGAGGAAAGACGATTTTACATATTTTTGCCAAATCTTTCTATTATTAAGTACGGCTGGTACCATCCTGATGTGTTTCGATTATTTCGAACAAGAGAGGTTTAATGCTTTCGAATTCATTGTATTAATTCTACTTTCTACTTGCAGTATGCTCTTTATGATCTCGGCTTATGATTTAATTGCCATGTATTTAGCTATTGAGCTTCAAAGTTTATGTTTTTACGTGATCGCAGCATCAAAAAGGAACTCTGAATTTTCCACGGAAGCCGGCTTGAAATATTTGATTTTAGGTGCATTTTCCTCTGGAATATTATTGTTTGGGTACGACTGGACAACTACCGATATATGTTATTTCCGTCAACATTACGGCTTATATTATGGGTATATGTTATTCCTGTAAACATTACGACTTATATTATGGGTGGATATTGTATCAAATTAGAATTTCCATCCGTTATGGCAATATTAGCCAATATATGAAT

Fln Alignment:

Contig25105___AVFPEIFLINATLILLIHGVVFSTSKKYDYPPLASNVGWLGLLSVLITMXXXXXXXXXXXXXFVEQGNNLTIAHFFWNHFLRKDDFTYFCQIFLLLSTAGTILMCFDYFEQERFNAFEFIVLILLSTCSMLFMISAYDLIAMYLAIELQSLCFYVIAASKRNSEFSTEAGLKYLILGAFSSGILLFGYDW
B1N7Z3_______________SIFPECILIFGLFLLLVIDVIFDKKKTTWFY-------FISLTSLVLSIAF-----------LLFQWREEPTIIFFGN--LQTNNFNKVFRFLILLCSTLCIPLSVEYIQCTEMAVTEFLLFILTATLGGMFLCGANDLTTIFVSLECLSLCSYLLSGYTKRDIRSNEAIMKYLLMGGTSSSILAYGLSW

SNPs (tot: 12)

Position	A %	C %	G %	T %	Probability
1165		50		50	1.0
1320		10		90	0.90298
1321	90		10		0.90298
1323			10	90	0.90298
1325	90	10			0.90298
1326		10	90		0.90298
1328	90			10	0.90298
1335			90	10	0.90298
1336		10		90	0.90298
1342		45		54	1.0
1429		90		10	0.90298
1512		80		20	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain