SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene22412

Length: 1640 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene22412

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	putative GTP-binding protein ERG [Oryza sativa Japonica Group]	-	-	0.0	66%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	28%
Sma3	GTP-binding protein ERG	-	-	2.304e-09	-

Source	Gene names
Sma3	At1g30960; ERG; F17F8.15; GSVIVT00030112001; OJ1734_E04.12; OsI_28232; OsI_30415; OsJ_26417; OsJ_26418; PHYPADRAFT_3190; POPTRDRAFT_589836; RCOM_1241400; VITISV_029539;

Source	GOs	Term	Type	Identity
Sma3	intracellular	GO:0005622	Cellular Component	-
Sma3	RNA binding	GO:0003723	Molecular Function	-
Sma3	GTP binding	GO:0005525	Molecular Function	-

Source	InterPros	Term	Type	Identity
Sma3		IPR002917	-	-
Sma3	K Homology domain, type 2	IPR004044	-	-
Sma3	Small GTP-binding protein domain	IPR005225	-	-
Sma3	GTP-binding protein Era	IPR005662	-	-
Sma3	K homology domain-like, alpha/beta	IPR015946	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT1G30960.1	GTP-binding family protein chr1:11037755-11039977 REVERSE LENGTH=437	64%
RefSeq	Arabidopsis thaliana	NP_174383.1	GTP-binding protein ERG [Arabidopsis thaliana]	64%
RefSeq	Populus trichocarpa	XP_002326628.1	predicted protein [Populus trichocarpa]	67%

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: D5AC54

Fln msg: query STOP codon is far from subject stop. Distance to subject end: 24 aas, your sequence is shorter than subject: 365 - 433

Fln protein: DDDEEEEAEEEEDDDDMGSEMPPTDSSIDDWFPGLSTEEKERRASELAKALLEASLQRPDDELDEEEEEEVREEDQKSLAVGIVGAPNAGKSTLTNYLVGTKVAAVSRKTNTTTHEILGVMTNGDTQILFFDTPGIMRSSSGRFYSADVTQRTESAWRSLFLYDVLLVMIDAHRHLAKPDPRVISLVKKLGEIVQPKQKRILCMNKVDLIEDKRDLLKVASEFGHLPGYDEFYMISGMRGSGVKNLVKYLMDQAVKRPWDEDPMLLDEEYLKSLSLEVVREKMLDCIHQEIPYTVDQRLIDWAPMRDGSLRIEQHLIVIKLGHRKILVGKNGSKIRQIGMAANEELRKLLKRTVHLIIQVRVKK*

Protein Length: 366

Fln nts: AGGACGACGACGAAGAAGAAGAAGCGGAAGAGGAAGAGGACGATGACGACATGGGTTCTGAAATGCCGCCGACTGATAGTTCTATTGATGATTGGTTTCCTGGATTGAGTACCGAAGAGAAAGAGAGGAGGGCTTCAGAACTGGCCAAGGCTTTGCTGGAAGCGTCTCTGCAACGGCCAGACGACGAACTCGATGAAGAAGAAGAAGAAGAGGTCAGGGAAGAGGACCAGAAGTCGTTGGCTGTCGGAATCGTTGGAGCTCCAAACGCCGGGAAATCGACATTGACGAATTATCTGGTTGGAACGAAGGTTGCTGCAGTTTCACGAAAGACAAACACAACAACTCATGAGATTCTTGGAGTGATGACAAATGGGGACACACAAATTCTTTTTTTTGATACACCAGGTATCATGCGCAGCAGTTCTGGCCGCTTTTACAGTGCGGATGTCACACAACGCACTGAAAGTGCATGGAGATCGTTATTTCTTTATGATGTACTACTAGTAATGATTGATGCTCATCGGCATCTTGCAAAGCCTGATCCGAGGGTAATATCTCTTGTGAAAAAGTTGGGAGAAATTGTGCAACCCAAACAAAAGCGAATCTTATGCATGAATAAGGTTGACTTAATTGAAGACAAAAGAGACTTATTAAAAGTTGCTTCCGAATTTGGACACCTTCCTGGATATGATGAATTTTACATGATATCTGGAATGAGAGGCTCTGGTGTCAAGAATCTTGTAAAATATTTAATGGATCAGGCTGTTAAGAGACCTTGGGATGAAGATCCCATGTTGTTGGATGAGGAGTACTTGAAAAGTTTATCTCTTGAAGTTGTACGAGAAAAGATGTTGGATTGTATTCATCAGGAAATACCATATACAGTGGATCAGCGCTTGATTGATTGGGCACCAATGCGAGATGGGTCTTTACGAATAGAGCAACATTTGATTGTGATTAAGCTCGGCCATCGAAAAATTCTTGTTGGAAAGAATGGATCTAAAATAAGACAGATTGGAATGGCAGCAAATGAAGAATTAAGAAAACTGTTAAAGAGGACTGTACACCTTATAATCCAAGTGAGGGTGAAGAAGTGA___GAATATTCATGGAGAGGGAATGGTACAATTAATACCCAAGCAAATGTGCTGGAAATGCGTTTACAAATATTTTGTTTCCTTGGCAATGGAGGAGACCTCAGCAATGTATAGGGTGGTGCAGATGATCCTTGTTAAGGTGGCTGGAACATGTTTATATGCTTTCAATTTCTTTAACAAGAGCAATTTGTTTCTCTGGCATCACAGAACACAGAACGTCATGTTTTGTGTTAGCCTTTTGTAAAATATAGAGATTGACATTATTGATTATTTATCATCATCTGAATTTTGTTCAGTCTTTTATGAACATTTGGTTAGCAGTCATATTAATATGGAAAGAAATCCTCAATTAGGGATAGGCCACCATGGCTTGCTGATCTGTATACTGTAGCATATGCAAAATTTTGACCAAAAATTCAGTACCCAGCATACACATAGCAGGAAAAGGATATGTACAAGGGTCAGACTGTTATGTAATAATTTCCAGATTTAGATTTTTCTGCAAAATTCAGAAACAGTGTTGGCAAGTATTTATACATAAAGCCA

Fln Alignment:

Contig22412___VGIVGAPNAGKSTLTNYLVGTKVAAVSRKTNTTTHEILGVMTNGDTQILFFDTPGIMRSSSGRFYSADVTQRTESAWRSLFLYDVLLVMIDAHRHLAKPDPRVISLVKKLGEIVQPKQKRILCMNKVDLIEDKRDLLKVASEFGHLPGYDEFYMISGMRGSGVKNLVKYLMDQAVKRPWDEDPMLLDEEYLKSLSLEVVREKMLDCIHQEIPYTVDQRLIDWAPMRDGSLRIEQHLIVIKLGHRKILVGKNGSKIRQIGMAANEELRKLLKRTVHLIIQVRVKK*EYSWR
D5AC54_______________VAVVGKPNVGKSTLLNQMIGQKLSIVTDKPQTTRHRILGLCSAPDYQMVLYDTPGVIQKEMHKL-DAMMMKNVHSATGNA---DCVLTVVD----VCKVPEKVNDLFEEDGVDLKKKPPTLLVLNKKDLMK-PGEIAKRMEWYEKFGGADHILSISAKYGHGVEDVKNWIVSKLPYGPAYYSKDIISEHPERFFVAEIVREKIFMQYRQEVPYACQVNVASYIARPTSKHFIEIEIVVEKGAQKIILIGKEGKALKLLATAARLDIEDFLQKQVYLEIKVKVKP---NWR

SNPs (tot: 22)

Position	A %	C %	G %	T %	Probability
27		25	75		0.996473
28		75		25	0.996473
29		25	75		0.996473
30	25		75		0.996473
302	20			80	0.995493
304			80	20	0.995493
306	80			20	0.995493
308		20	80		0.995493
312			80	20	0.995493
313			80	20	0.995493
325			25	75	0.996473
804		14		85	0.993533
930	14		85		0.993533
931		14	85		0.993533
932	14		85		0.993533
934		85		14	0.993533
1281	85			14	0.993533
1282			85	14	0.993533
1525	10	90			0.90298
1526		90		10	0.90298
1527	90			10	0.90298
1557	14	85			0.993533

SSRs (tot: 2)

Start position	End position	Sequence	Length
12	24	GAA GAA GAA GAA G	13
195	211	GAA GAA GAA GAA GAA GA	17

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain