SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene18874

Length: 1462 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene18874

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	3-isopropylmalate dehydratase, putative n=1 Tax=Ricinus communis RepID=B9SXE3_RICCO	-	-	0.0	65%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	88%
Sma3	Putative 3-isopropylmalate dehydratase small subunit	-	-	9.17e-09	-

Source	Gene names
Sma3	At2g43090; At2g43100; At3g58990; C740ERIPDM; CHLREDRAFT_126865; F17J16_40; GSVIVT00012224001; Grc000034; IPMDHSU; LEU1; LEU1S; OJ1003_B06.26; Os02g0655300; OsI_08324; OsJ_07781; P0519E06.10; PHYPADRAFT_47040; PHYPADRAFT_47201; POPTRDRAFT_415236; RCOM_1389

Source	GOs	Term	Type	Identity
Sma3	3-isopropylmalate dehydratase complex	GO:0009316	Cellular Component	-
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	chloroplast stroma	GO:0009570	Cellular Component	-
Sma3	3-isopropylmalate dehydratase activity	GO:0003861	Molecular Function	-
Sma3	leucine biosynthetic process	GO:0009098	Biological Process	-
Sma3	response to salt stress	GO:0009651	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Aconitase A/isopropylmalate dehydratase small subunit, swivel	IPR000573	-	-
Sma3	3-isopropylmalate dehydratase, small subunit, subgroup	IPR011827	-	-
Sma3		IPR012305	-	-
Sma3	Aconitase/3-isopropylmalate dehydratase, swivel	IPR015928	-	-
Sma3	Aconitase/isopropylmalate dehydratase	IPR015937	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT2G43090.1	Aconitase/3-isopropylmalate dehydratase protein chr2:17918957-17919712 FORWARD LENGTH=251	70%
RefSeq	Arabidopsis thaliana	NP_181837.1	Aconitase/3-isopropylmalate dehydratase protein [Arabidopsis thaliana]	70%
RefSeq	Populus trichocarpa	XP_002303495.1	predicted protein, partial [Populus trichocarpa]	81%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: A9NMS1

Fln msg: Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 266 - 291

Fln protein: MSMAAINATRSSSVSTNLTHFAANTRSKLALLHIRHISLHSLHKNALNLHFSTPRKNRISTFSVRNPPRAIASQSYDAPTEPNKKIFHGKCFVVDDNIDTDQIIPAEYLTLVPSKPDEYEKLGSYAMIGLPARYGRFIEDGQTKTQYSIVIAGANFGCGSSREHAPVALGAAGVKAVVAEYYARIFFRNSVATGEIYPLESECRVCDECKTGDIVTVEIEGNKLINHTTGKEYVLKPIGDAGPVIDAGGIFEYARKTGMIAAPSS*

Protein Length: 267

Fln nts: ATGGGGGCGAAAGATTTATAAATCTTTGTCAATGGTTAACGACCCTGCGCTGCAAGCTCGATAGCCGATAACACACGCGCCCGGTGACTAGATGGCGCTATCGAACTTACAGCTAAGCCGGTATTTAATAGGTGTTAGCCCTTGCCAAAATCTTCACCATACAATGATACTCCATAATCCTACGACTTTTCTGTGAACTCTTACTTTCGGAAGCTCTTGTCAGTCTGGTTCATAGG_-_ATGTCAATGGCGGCTATAAACGCTACGCGGTCTTCATCGGTAAGCACAAATTTGACGCATTTTGCTGCAAATACACGCTCAAAGTTAGCTCTGCTTCATATTCGACATATCTCACTGCATTCTCTGCACAAAAATGCCCTAAACTTGCATTTTTCTACCCCACGAAAAAACAGAATATCAACATTCTCTGTACGGAATCCCCCAAGAGCAATAGCATCTCAATCTTATGATGCACCCACAGAGCCAAACAAGAAAATTTTTCATGGAAAATGCTTTGTGGTAGACGACAACATAGACACTGATCAAATCATCCCAGCAGAATATTTAACCCTTGTGCCCTCCAAACCAGACGAATACGAAAAGTTGGGCAGTTATGCCATGATTGGCCTTCCTGCTAGATATGGCAGATTCATTGAAGATGGCCAGACTAAAACCCAATATTCCATTGTCATTGCTGGGGCCAATTTTGGATGTGGATCATCCAGAGAGCATGCCCCTGTGGCTCTTGGGGCGGCGGGAGTGAAGGCTGTAGTGGCGGAGTACTATGCCAGGATATTTTTCAGGAACTCTGTGGCCACCGGTGAGATCTATCCACTGGAGAGCGAGTGCCGGGTTTGCGACGAATGCAAAACTGGTGATATTGTCACTGTGGAGATTGAGGGAAATAAGCTTATCAATCACACCACTGGAAAGGAGTATGTATTGAAGCCTATTGGAGATGCCGGGCCTGTGATCGATGCTGGCGGGATATTTGAATACGCGCGCAAAACAGGGATGATTGCTGCTCCATCATCTTGAGACCTGATGGGGACTTCCCAAACTGTGGAAAGAAGATGAGTGTAGTGATGATTGTTGCTCCATCAGCTGGAGACCTGATGGGAACTTCCCAAACCGTGAAAAGAAAACGAGTGTAGTAAACTTATCAGCATGTTATTACATTTGAAATCAAAGAACAGTCATTGTCTTTCATGGTTAGTGTCTGGTAAGAAGCAAATATTTTTTATAAAATTTGAAACTCCTTTTGACGAGGACAATATTTGAGAACAAAATTGTTTGTTGGTTTGTTTGATTTTGGAATTTACTGTTCAAGCCTCCGGTTTATTGGACCGTAAAAGTCGTTTAGCTGATTTGTTTTAGTACTCTGTTAGAATTTTTCACAATCTTAACCTTTTGCATTAAGATGTATTAGCCTATTGCATTCATGGTGATCACATCATTATTTAAAC

Fln Alignment:

Contig18874___RMSMAAINATRSSSVSTNLTHFAANTRSKLAXXXXXXXXXXXXXKNALNLHFSTPRKNRISTFSVRNPPRAIASQSYDAPTEPNKKIFHGKCFVVDDNIDTDQIIPAEYLTLVPSKPDEYEKLGSYAMIGLPARYGRFIEDGQTKTQYSIVIAGANFGCGSSREHXXXXXXXXXXXXXXXXXXXRIFFRNSVATGEIYPLESECRVCDECKTGDIVTVEIEGNKLINHTTGKEYVLKPIGDAGPVIDAGGIFEYARKTGMIAAPSS
A9NMS1_______________RMTTAAINAMKSLSVSTNLMHFPAKTSSKLALPQTQQISRQSLHKNVLNFHLSTPRNNRISTYSVPNPPRAVASQSYDALTEPNKKIFHGKCFVVDDNIDTDQIIPAEYLTLVPSKPDEYEKLGSYAMIGLNAKYGRFIEDDQIKTQYPIVIAGANFGCGSSREHAPVALGAAGVKAVVAEYYARIFFRNSVATGEIYPLESECRVCDECKTGDIVTVEIEGNKLINHTTGKEYVLKPIGDAGPVIDAGGIFEYARRTGMIAAPSS

SNPs (tot: 13)

Position	A %	C %	G %	T %	Probability
45	33	66			0.997452
46		66		33	0.997452
47		66	33		0.997452
48			33	66	0.997452
50		66	33		0.997452
147		66		33	0.997452
149	66			33	0.997452
152	66	16		16	0.988813
153	16			83	0.994512
154		66	16		0.988813
155			33	66	1.0
156		16	33	50	1.0
157	16	50	33		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain