SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene17289

Length: 2209 nt

UniGene Fasta

>sp_v3.0_unigene17289
ACTGTACCTTCTTCTGGGATCAACAAACCTCAGAAAGCCTAGCATCTTCTTGGATCAAGAACGGTCGAAACTCTATCTTCTTCTTGGATCAGCGAACCCTCGAAACTCGATTGTGAGAAGGCGTTGGATCCCGTGCCACAAGACCGACATGGCTTGCGTTGCAGAATATGTCTTCTCTATCTCTGTATCTGATGAAAATCTCCAAGAAGCGCGACAAAGCGTATACGTGTTCTCTGTGATATGTCCTCTATAGTCATATGCAGGGACATTGGGCCAAGTTTTAGAATGCTGGGACCGAAAATCAAAGGACCTTGTAGCTATTAAAATCGTTCGAGGCCTACAAAAGTACCGGGAGGCAGCAATGATTGAAATTGATGTGCTGCGTGAAGTTGCCAAGCATGATAGAACTGGTACACGGTATGAAAGAATGAATATCTGTCGAGATATGTCATTTAGTTAATCACTCCACAGTTTTTAATAGATATAGCCATTCTGAGTGATTATTTGCTTTAGACTCCTTAAGGTACAGTATCTCACTGGTCGGTTGATGTGCGTGACTTGTTTGATACAGTTGTGTGCAAATGCGCGACTGGTTTGACTATAGGAATCACACGTGTATCGTGAGTTCTCAGCAGAACTTAATTATTTTCGTTAGTGAAATTTTAGGCATTTTAAATGGCCTCTCAAGGATCTTCTTTTGGTTGTTTCTAGAGAAGTTTTTAGTTTGTTGATGAATGTAAATTATCTTAACAGGTTTTTGAAAGACTTGGGCCAAGCTTATATGACTTTCTTCAGAAAAATAACTATCGCCCATTTCCCATTGATCTTGTTAAGGAGGTTGGCAGGCAACTTTTGCAGTCTGTAGCATGTGTGTAACATCGCTTCTTTTTCCCTTATTAGAGAGCTTTATTGGCTGACCAGTAATATTATCATTTCAGAGTTGCATATATGATCTTTATTATCAATTAGGTCCTGAGAAAATGTCTATCCATTATAAGTGATATTCATTTGAGTTTAATAACAATCTGGATTGGCAGATATGCATGACTTGAAGCTCATTCACACAGATTTAAAGCCAGAAAATACACTTTTTGTTTCTTCAGACATTGTGAAGATACCATATAAGGGTTTGGAGAAGTCATCACAAGAAATATGCAACAGATTGCCAAAGTCAAGTGCAATAAAGTTGATTGATTTTGGAAGTACCACTTTCGAAGGAGAAGATCATACATCAGTGATCTCTACAAGGCATTATCGAGCTCCCGAAGTTATATTGGGACTTGGATGGAGCTACCCCTCTGATATTTGGAGTGTAGGATGCATACTTGTGGAGCTTTGCTTGGGAAAGACACTATTTCAAACCCATGAGAATTTGGAACATCTTGCCATGATGGAAAGGGTTTTGGTTGCATTGCCACATCGTATGATAAGCAAGCAGAAATTTGATCATGGAGCATGTGGACCATTCAGCTGGTCTACTTATAGACTTGCTGCAAGGACTTCTCAAGTTTGAGCCTTCGGAACGTTTGAAAGCTTCAGAAGCTTTGAGTCATCCATTCTTCTTAGAGGGTTCCAAATTTCATAAATATGGTACATAGAAATCATGAGAATTCTTGATTCGCTGCATGGGACAATGTTGGATTGTTATCAACTTCGTTGGTCTCAGGTGCCAGGCATCATCGGTCCCTAGGCTTCTGAACCAGGAGAACTTCGAGACATCCATTCTTCGAAGATAATGCTGTCTGCCTAACGATAATCCATTACAGCATAAAGTTTGGGCTCTCCCTGCATGAGGCAAATATGAGTGGGATTGACTTTGTTGGTCTCTGCCACGAGGGTCCCCAATCGAGTTAAAAGCGGATGGGATATGTACTGGATTGGGACAATTTTTTGGGTGGCTATGTTCCGTTCTGGAGATGGGATCTGTGCGCAGTATTTTAAGGATTATGGCTCGGACACCTAGCATCCTAACGGTACATTCAATTAGGAGTCGAAAGCAAGGTAAATGGGATATTCCCTGTTTGGGACAAATTTTGGGTGCCTATGTTTTAGTGTGGAGATGGCTCTGTGCCCCGGGGCTCTAAGAAATTATTTAGTCAGATATCTTGCCATCACAATAAACTTATTTGGCTTATAGACATTGTAAAATGAGAACCGTTGGTGTCTAAGTCTACTTTTACATTATATTGAATGTTTAGTATTTTTCAAC

Ace file of the UniGene sp_v3.0_unigene17289

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Serine/threonine-protein kinase AFC2 n=4 Tax=Arabidopsis RepID=AFC2_ARATH	-	-	0.0	70%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	76%
Sma3	Afc, putative	-	-	1.22e-15	-

Source	ECs	Term	Type	e value	Identity
Sma3	Dual-specificity kinase.	EC:2.7.12.1	-	4.98e-23	-

Source	Gene names
Sma3	AFC1; AFC2; AFC3; AME2; AME3; AT3G53570; At3g53570; At4g24740; At4g32660; CHLREDRAFT_130101; F22K18.60; F4D11.140; F4P12_270; GSVIVT00015960001; GSVIVT00023119001; GSVIVT00030742001; GSVIVT00032520001; LOC_Os12g27520; MICPUCDRAFT_3523; MICPUN_57150; OSTLU

Source	GOs	Term	Type	Identity
Sma3	protein kinase activity	GO:0004672	Molecular Function	-
Sma3	protein serine/threonine kinase activity	GO:0004674	Molecular Function	-
Sma3	protein binding	GO:0005515	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	mRNA processing	GO:0006397	Biological Process	-
Sma3	protein phosphorylation	GO:0006468	Biological Process	-
Sma3		GO:0045449	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Serine/threonine- / dual-specificity protein kinase, catalytic domain	IPR002290	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT4G24740.1	AFC2, AME1, FC2 FUS3-complementing gene 2 chr4:12754729-12757653 REVERSE LENGTH=427	70%
RefSeq	Arabidopsis thaliana	NP_194205.1	serine/threonine-protein kinase AFC2 [Arabidopsis thaliana]	70%
RefSeq	Populus trichocarpa	XP_002308478.1	predicted protein [Populus trichocarpa]	72%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LL66

Fln msg: Separated hits, possible frame ERROR between 430 and 754, and possible frame ERROR between 1435 and 1437, Unexpected stop codon in the beginning of your sequence, your sequence is longer than subject: 529 - 459

Fln protein: LYLLLGSTNLRKPSIFLDQERSKLYLLLGSANPRNSIVRRRWIPCHKTDMACVAEYVFSISVSDENLQEARQSVYVFSVICPL*SYAGTLGQVLECWDRKSKDLVAIKIVRGLQKYREAAMIEIDVLREVAKHDRTGTRYERxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxVFERLGPSLYDFLQKNNYRPFPIDLVKEVGRQLLQSVACV*HRFFFPY*RALLADQ*YYHFRVAYMIFIIN*VLRKCLSIISDIHLSLITIWIGRYA*LEAHSHRFKARKYTFCFFRHCEDTI*GFGEVITRNMQQIAKVKCNKVD*FWKYHFRRRRSYISDLYKALSSSRSYIGTWMELPL*YLECRMHTCGALLGKDTISNP*EFGTSCHDGKGFGCIATSYDKxxQKFDHGACGPFSWSTYRLAARTSQV*AFGTFESFRSFESSILLRGFQIS*

Protein Length: 530

Fln nts: ACTGTACCTTCTTCTGGGATCAACAAACCTCAGAAAGCCTAGCATCTTCTTGGATCAAGAACGGTCGAAACTCTATCTTCTTCTTGGATCAGCGAACCCTCGAAACTCGATTGTGAGAAGGCGTTGGATCCCGTGCCACAAGACCGACATGGCTTGCGTTGCAGAATATGTCTTCTCTATCTCTGTATCTGATGAAAATCTCCAAGAAGCGCGACAAAGCGTATACGTGTTCTCTGTGATATGTCCTCTATAGTCATATGCAGGGACATTGGGCCAAGTTTTAGAATGCTGGGACCGAAAATCAAAGGACCTTGTAGCTATTAAAATCGTTCGAGGCCTACAAAAGTACCGGGAGGCAGCAATGATTGAAATTGATGTGCTGCGTGAAGTTGCCAAGCATGATAGAACTGGTACACGGTATGAAAGAATxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxGGTTTTTGAAAGACTTGGGCCAAGCTTATATGACTTTCTTCAGAAAAATAACTATCGCCCATTTCCCATTGATCTTGTTAAGGAGGTTGGCAGGCAACTTTTGCAGTCTGTAGCATGTGTGTAACATCGCTTCTTTTTCCCTTATTAGAGAGCTTTATTGGCTGACCAGTAATATTATCATTTCAGAGTTGCATATATGATCTTTATTATCAATTAGGTCCTGAGAAAATGTCTATCCATTATAAGTGATATTCATTTGAGTTTAATAACAATCTGGATTGGCAGATATGCATGACTTGAAGCTCATTCACACAGATTTAAAGCCAGAAAATACACTTTTTGTTTCTTCAGACATTGTGAAGATACCATATAAGGGTTTGGAGAAGTCATCACAAGAAATATGCAACAGATTGCCAAAGTCAAGTGCAATAAAGTTGATTGATTTTGGAAGTACCACTTTCGAAGGAGAAGATCATACATCAGTGATCTCTACAAGGCATTATCGAGCTCCCGAAGTTATATTGGGACTTGGATGGAGCTACCCCTCTGATATTTGGAGTGTAGGATGCATACTTGTGGAGCTTTGCTTGGGAAAGACACTATTTCAAACCCATGAGAATTTGGAACATCTTGCCATGATGGAAAGGGTTTTGGTTGCATTGCCACATCGTATGATAAGCAxxxGCAGAAATTTGATCATGGAGCATGTGGACCATTCAGCTGGTCTACTTATAGACTTGCTGCAAGGACTTCTCAAGTTTGAGCCTTCGGAACGTTTGAAAGCTTCAGAAGCTTTGAGTCATCCATTCTTCTTAGAGGGTTCCAAATTTCATAAATATGGTACATAGAAATCATGAGAATTCTTGATTCGCTGCATGGGACAATGTTGGATTGTTATCAACTTCGTTGGTCTCAGGTGCCAGGCATCATCGGTCCCTAGGCTTCTGAACCAGGAGAACTTCGAGACATCCATTCTTCGAAGATAATGCTGTCTGCCTAACGATAATCCATTACAGCATAAAGTTTGGGCTCTCCCTGCATGAGGCAAATATGAGTGGGATTGACTTTGTTGGTCTCTGCCACGAGGGTCCCCAATCGAGTTAAAAGCGGATGGGATATGTACTGGATTGGGACAATTTTTTGGGTGGCTATGTTCCGTTCTGGAGATGGGATCTGTGCGCAGTATTTTAAGGATTATGGCTCGGACACCTAGCATCCTAACGGTACATTCAATTAGGAGTCGAAAGCAAGGTAAATGGGATATTCCCTGTTTGGGACAAATTTTGGGTGCCTATGTTTTAGTGTGGAGATGGCTCTGTGCCCCGGGGCTCTAAGAAATTATTTAGTCAGATATCTTGCCATCACAATAAACTTATTTGGCTTATAGACATTGTAAAATGAGAACCGTTGGTGTCTAAGTCTACTTTTACATTATATTGAATGTTTAGTATTTTTCAAC

Fln Alignment:

Contig17289___GTLGQVLECWDRKSKDLVAIKIVRGLQKYREAAMIEIDVLREVAKHDRTGTRYERMxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxVFERLGPSLYDFLQKNNYRPFPIDLVKEVGRQLLQSVACV*HxRNLIMEHVDHSAGXXXXXXXXXXKFEPSERLKASEALSHPFFLE
B8LL66_______________GTFGQVLECWDRETREFVAVKIVRGLQRYREAAMIEIDVLREVAKYDRSGTRCVQMxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxVFEKLGPSLYDFLRKNNYHPFPIDIVRELGKQLLESVAYMHHxRNLVMERADHSAGALIDLLQGLLKYEPSERLEAQEALRHPFFTE

SNPs (tot: 56)

Position	A %	C %	G %	T %	Probability
457	20			80	0.995493
459	80		20		0.995493
460	80		20		0.995493
461			20	80	0.995493
464	20	80			0.995493
466		60	40		1.0
482			75	25	0.996473
488	25		75		0.996473
525			75	25	0.996473
539		25		75	0.996473
556		25		75	0.996473
557			75	25	0.996473
566	25		75		0.996473
586	25	75			0.996473
664			33	66	0.997452
690	33		66		0.997452
709			33	66	0.997452
712	66			33	0.997452
729	33		66		0.997452
902			75	25	0.996473
918	25		75		0.996473
920		50		50	1.0
923	25		75		0.996473
926	75			25	0.996473
929	25			75	0.996473
948		25		75	0.996473
956	20	80			0.995493
965		60		40	1.0
974		80		20	0.995493
975		80	20		0.995493
977		20	80		0.995493
992	25	75			0.996473
1001		16	16	66	0.988813
1025	83			16	0.994512
1039			87	12	0.992554
1071		14		85	0.993533
1119	66			33	0.997452
1154	25			75	0.996473
1159	75		25		0.996473
1248	60	40			1.0
1300	25			75	0.996473
1301	25	75			0.996473
1304	75			25	0.996473
1447	66	33			0.997452
1450			16	83	0.994512
1451			16	83	0.994512
1472	83		16		0.994512
1543		83		16	0.994512
1629		66	33		1.0
1686	25		75		0.996473
1695		66		33	0.997452
1855	20		80		0.995493
1886		66		33	0.997452
1975		66	33		0.997452
2104		33		66	0.997452
2115		33	66		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain