SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene13508

Length: 1214 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v3.0_unigene13508 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	adenine nucleotide alpha hydrolase domain-containing protein kinase [Arabidopsis thaliana] gb\|ABE65860.1\| protein kinase family protein [Arabidopsis thaliana] gb\|AEC07567.1\| adenine nucleotide alpha hydrolase domain-containing protein kinase [Arabidopsis	-	-	0.0	62%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	41%
Sma3	E3 ubiquitin ligase	-	-	3.773e-18	-

Source	ECs	Term	Type	e value	Identity
Sma3	Non-specific protein-tyrosine kinase.	EC:2.7.10.2	-	5.455e-06	-
Sma3	Transferases, Transferring phosphorous-containing groups, Protein-serine/threonine kinases.	EC:2.7.11.-	-	4.182e-11	-
Sma3	Ligases, Forming carbon-nitrogen bonds, Acid--D-amino-acid ligases (peptide synthases).	EC:6.3.2.-	-	1.926e-07	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Tryptophan metabolism	00380		1.926e-07	%
Sma3	Biosynthesis of siderophore group nonribosomal peptides	01053		1.926e-07	%

Source	Gene names
Sma3	57h21.16; AT1G79620; AT4g31220; At1g17540; At1g78940/YUP8H12R_26; At1g79620; At1g79620/F20B17_5; At2g07020; At2g19410; At2g24370; At2g45910; At3g20200; At3g20200/MAL21_24; At3g49060; At4g25160; At4g31230; At5g12000; At5g51270; At5g57035; At5g61550; At5g61

Source	GOs	Term	Type	Identity
Sma3	ubiquitin ligase complex	GO:0000151	Cellular Component	-
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	membrane	GO:0016020	Cellular Component	-
Sma3	protein kinase activity	GO:0004672	Molecular Function	-
Sma3	protein serine/threonine kinase activity	GO:0004674	Molecular Function	-
Sma3	protein tyrosine kinase activity	GO:0004713	Molecular Function	-
Sma3	ubiquitin-protein ligase activity	GO:0004842	Molecular Function	-
Sma3	receptor activity	GO:0004872	Molecular Function	-
Sma3	calcium ion binding	GO:0005509	Molecular Function	-
Sma3	protein binding	GO:0005515	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	protein phosphorylation	GO:0006468	Biological Process	-
Sma3	response to stress	GO:0006950	Biological Process	-
Sma3	protein ubiquitination	GO:0016567	Biological Process	-
Sma3	modification-dependent protein catabolic process	GO:0019941	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	EGF-type aspartate/asparagine hydroxylation site	IPR000152	-	-
Sma3	JAB1/Mov34/MPN/PAD-1	IPR000555	-	-
Sma3	Drug/metabolite transporter	IPR000620	-	-
Sma3	RNA polymerase II, heptapeptide repeat, eukaryotic	IPR000684	-	-
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Epidermal growth factor-like domain	IPR000742	-	-
Sma3	Serine-threonine/tyrosine-protein kinase catalytic domain	IPR001245	-	-
Sma3	Tetratricopeptide TPR-1	IPR001440	-	-
Sma3	Leucine-rich repeat	IPR001611	-	-
Sma3	EGF-like calcium-binding	IPR001881	-	-
Sma3	U box domain	IPR003613	-	-
Sma3	Domain of unknown function DUF250	IPR004853	-	-
Sma3	UspA	IPR006016	-	-
Sma3	Aspartate/ornithine carbamoyltransferase	IPR006130	-	-
Sma3	EGF-like domain	IPR006209	-	-
Sma3	Epidermal growth factor-like	IPR006210	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	Tetratricopeptide-like helical	IPR011990	-	-
Sma3	Tetratricopeptide repeat-containing domain	IPR013026	-	-
Sma3	Leucine-rich repeat-containing N-terminal, type 2	IPR013210	-	-
Sma3	Rossmann-like alpha/beta/alpha sandwich fold	IPR014729	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-
Sma3	EGF-like calcium-binding, conserved site	IPR018097	-	-
Sma3	Ribosomal protein L19, conserved site	IPR018257	-	-
Sma3	Tetratricopeptide repeat	IPR019734	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT2G24370.1	Protein kinase protein with adenine nucleotide alpha hydrolases-like domain chr2:10369979-10373063 REVERSE LENGTH=788	62%
RefSeq	Arabidopsis thaliana	NP_180014.2	adenine nucleotide alpha hydrolase domain-containing protein kinase [Arabidopsis thaliana]	62%
RefSeq	Populus trichocarpa	XP_002330780.1	predicted protein, partial [Populus trichocarpa]	61%

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LPB1

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 75 aas, putative chimeric sequence, your sequence is shorter than subject: 345 - 444

Fln protein: FSRSLVRNRSASNSIKTSTSEKATAAENKDVISLTFAELEDTNDTSDQRFRRYSIEQIKFATDFFAESLKIGEGGYGPVYKANLDHTTVAIKVLQADTAQGREEFQREIEILSRIHHPNMVMLLGACPDRGCLVYEYMENGSLEDQLFHRGDGYSLPWFIRFRMASEIASALHFLHTATPEPIIHRDLKPANILIDHNFVSKISDVGLAKLIPPPVSCALTEYKMTVLAGTFYYMDPEYVRTGTLFPASDVYAMGIIFLQLLTAKSPMGIMEDVQKAIQEGSFNEILDKTAGDWPLRETEEFAELALKCTELRRRDRPDMKTVILPKLNQFRDLKNAHFEDCIS*

Protein Length: 346

Fln nts: TTTTCAAGAAGCCTGGTGAGAAATAGATCTGCAAGTAACAGCATAAAGACAAGCACATCTGAAAAGGCAACTGCTGCAGAGAACAAGGATGTTATAAGTCTAACGTTCGCGGAACTGGAGGACACAAATGATACTTCTGATCAGAGATTTAGAAGATATTCAATAGAACAAATTAAATTTGCTACGGATTTCTTCGCTGAATCACTTAAAATAGGCGAGGGAGGATATGGCCCAGTGTACAAGGCCAATTTAGACCATACAACTGTGGCTATCAAAGTTCTCCAAGCTGATACAGCTCAAGGCAGAGAGGAATTCCAACGCGAGATTGAGATTCTAAGCCGCATACATCACCCCAACATGGTTATGCTGCTTGGCGCTTGCCCTGATCGCGGATGTCTAGTTTATGAATATATGGAAAATGGCAGTTTAGAGGATCAGTTGTTCCACAGGGGTGACGGATATTCTCTTCCATGGTTTATCCGCTTTCGAATGGCTTCAGAAATAGCTAGCGCTCTTCATTTTCTTCACACCGCCACGCCAGAGCCAATCATTCATCGTGATCTAAAGCCTGCCAACATATTGATAGATCACAATTTTGTGAGCAAAATCAGCGATGTAGGACTAGCTAAGCTCATTCCTCCTCCAGTCTCTTGCGCTCTTACTGAATACAAGATGACAGTCCTTGCTGGCACCTTCTATTATATGGATCCAGAGTACGTACGGACCGGAACATTGTTTCCTGCGTCTGATGTATATGCTATGGGAATCATTTTCCTGCAATTGCTCACAGCCAAATCCCCAATGGGTATTATGGAGGACGTTCAAAAAGCCATCCAGGAGGGCTCATTCAATGAAATTCTGGACAAAACGGCTGGGGACTGGCCTCTTAGGGAAACTGAAGAGTTTGCTGAACTTGCCCTCAAATGCACAGAATTGCGGCGCCGAGACAGACCGGATATGAAGACAGTTATTCTGCCAAAATTAAATCAGTTCAGAGATCTCAAAAATGCACATTTTGAGGACTGCATCTCCTAG___CTGCTAGCATTCTCCATTACTTCAGTCGGGTTCTCCCATTTGCAATATTTTCATTGAGAACTTGAATTATAAGAAAAAATTCGTCCTTCATACGAATTTTTCCTTTTTTGTAAATTGCCCTGGCGGCAAACATTCTTACCATGTACAAATTTCAAACCGTCAGATGCTTAATAACATTT

Fln Alignment:

Contig13508___RSASNSIKTSTSEKATAAENKDVISLTFAELEDTNDTSDQRFRRYSIEQIKFATDFFAESLKIGEGGYGPVYKANLDHTTVAIKVLQADTAQGREEFQREIEILSRIHHPNMVMLLGACPDRGCLVYEYMENGSLEDQLFHRGDGYSLPWFIRFRMASEIASALHFLHTATPEPIIHRDLKPANILIDHNFVSKISDVGLAKLIPPPVSCALTEYKMTVLAGTFYYMDPEYVRTGTLFPASDVYAMGIIFLQLLTAK---SPMG--IMEDVQKAI---QEGSFNEILDKTAGDWPLRETEEFAELALKCT
B8LPB1_______________KDAKNAIK----EKEAAIKERDEAR----ERLKRGAPPGRRFTRYTIQELKAATNNFSEDAVIGEGCYGIVYKGQFHVTPVAIKLLKVNWFEGSSRFQREMDRLSSIKHPRLVMLMGACPDGGFIIYEYMPRGSLEDRLRCKDGTPPLPWFDRMRIAAEICEGLLFMHSIQPEPIVHHDLKPSNILLDNDLGSKISDFGLVRLV------------------------SDRSRLQNLTPESDVYRFGILILQLLVGEPTIEPMFKILIESVAAALADDDEEEFKYVLDED-GRWPLEQARQLAEIALECT

SNPs (tot: 2)

Position	A %	C %	G %	T %	Probability
1150		66		33	0.997452
1152		33		66	0.997452

Back

Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain