SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene10552

Length: 1954 nt

UniGene Fasta

>sp_v3.0_unigene10552
AATGTTTTCTCACTCAAAATCCATCTGTGAGCTGTTTTAGGCCCCGATCCGAGGCAGCAACGGTCCAGGAACCGTAGAAAGCTTTAAAACGAGCTCTGAAACGCCTCTGGGTGCTCGGATATAGGATTTTATTCAGCAGGAAGAGAAGAAACGATGGAGCGTGCGTGGTTTGCTATCTTGATTATTTGTGCATTAGGGAGCATGGTCTGTGCAGTGGAAGGGCTGGGGGTGAATTGGGGCACCATGGCATTGCATCCGTTGCCTCCTGATATCGTAGTTCAGATGCTGAAAGACAATGGAATAAAGAAGGTGAAGCTATTTGATGCGGATTCGAACACTCTCCAGGCTTTGGCAAATACTGACATTGAGGTCATGGTGGCTATTCCCAACAATATGTTGCAGCATCTCAGCGACAGTTATAAGGCTGCAGAGAAATGGGTGGACAAGAATGTCACCAGATATAACTTCTCTGGTGGTGTTAACATCAAATATGTTGCAGTTGGAAATGAGCCTTTTCTGTCAGCATACAATGGCTCATATCTGAATAATACATTTCCTGCTCTGCAAAATATTCATAAAGCTCTTGTCAAAGCAGGTTTAGCAGACCAAATAAAAGTTACTGTACCTCTCAATGCAGATGTTTACAATTCCCCAACTGACAATGCAGTACCATCTGCTGGGGATTTTAGAGCAGATATTCATGACCTCATGAGCCAAATTGTGACATTTTTGAATGATAATGGATCACCCTTTACGGTCAATATCTATCCCTTTCTAAGCTTATATGCTGATGAAAACTTCCCCACAGAGTTTGCCTTCTTTGATGGTGATAGTCAGCCAGTAGTTGATGGGAATATCCAGTACACCAATGTCTTTGATGCAAATCTTGATACATTAGTTTGGTCTCTAAAGAAGGCAGGGGTCCCTAATATGACAATCATTGTGGGAGAAGTAGGTTGGCCCACAGATGGTGATAAGCATGCCACTGCTGCAGCTGCTCAGCGGTTCAATCAGGGCCTGTTGAAGCATATAACTAGTAACCAAGGAACACCCATGCGGCCTGGAAATATTGAAGCTTACCTATTTGGCCTTCTTGATGAGGATGCTAAGAGTGTTGCACCTGGTAACTTTGAAAGGCATTGGGGCATTTTTGGGTACGATGGTCAGCCAAAATATGAGCTGGATCTTTCAGGTCCATTGCAGAATGGAGGGTTAGTTCCTGCCAAGAATGTCAAGTATTTAGCTCCAAAGTGGTGTGTGTTCAAGACAAATGCTACAGACCAGTCCAAGATACTTGACAGCATCAAGTATGCTTGTACATATTCAGACTGCACTGCCATGGGTTATGGGTCGTCATGTAACAACTTGGATTTGTATGGCAATGCATCCTATGCATTCAACATGTACTTTCAAGTGATGAATCAATATGATATTAACTGCGATTTTACTGGTCTGGCAGTGATAACAGAGCAAAATGCATCACAGGGAACTTGCAAATTTCCAATAGGGATTGCATATGGTGCAGCAGAGAGGTCCATCAAGATTCATTCTATTCTGGCCGCTGTCCTCTTGGGTGTTGTTACATTTATCCTGTCATTCTTATAGGAATTTGGATCCGTCAATTCATTCTTTTTATATGCAAATCCTTCTCTTTCCTCCCTTCCTCCCCTTTGACTTAGTCTACAAAGATATGCATTAATTTTTTGTAACCACATACTCAAACTATTGCAATAAAGCGATAGAACCATACCATTATGTATTTGATTCTTAACATTTTATACGAGAAAGTAAGTCATGTTATTGCTACAAAATCAATTTGCCTCGCTGACAAGAATTTTATGGACAAATATTTACTTCAAGCTCACCGTTTTCAGTTAGTGATGTCATTTTGTATGAGCTTAATAGTACCATTTATGTGACTTGATATCAATGAGATCTATTCGTGTGAATTTTG

Ace file of the UniGene sp_v3.0_unigene10552

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Glycoside hydrolase, family 17; X8 [Medicago truncatula]	-	-	0.0	63%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	61%
Sma3	Glucan endo-1,3-beta-glucosidase 5	-	-	3.779e-20	-

Source	ECs	Term	Type	e value	Identity
Sma3	Glucan endo-1,3-beta-D-glucosidase.	EC:3.2.1.39	-	2.527e-24	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Starch and sucrose metabolism	00500		2.527e-24	%

Source	Gene names
Sma3	31.t00002; AT4g17180; At1g64760; At2g19440; At3g04010; At3g24330; At4g17180; At4g17180/dl4625w; At4g31140; At5g18220; At5g20870; At5g58090; At5g58480; At5g64790; BG2-1; BG2-2; BG2-3; F13O11.7; F6E21.60; GSVIVT00013097001; GSVIVT00016351001; GSVIVT00016795

Source	GOs	Term	Type	Identity
Sma3	extracellular region	GO:0005576	Cellular Component	-
Sma3	cell wall	GO:0005618	Cellular Component	-
Sma3	vacuole	GO:0005773	Cellular Component	-
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	plant-type cell wall	GO:0009505	Cellular Component	-
Sma3	anchored to membrane	GO:0031225	Cellular Component	-
Sma3	anchored to plasma membrane	GO:0046658	Cellular Component	-
Sma3	hydrolase activity, hydrolyzing O-glycosyl compounds	GO:0004553	Molecular Function	-
Sma3	glucan endo-1,3-beta-D-glucosidase activity	GO:0042973	Molecular Function	-
Sma3	cation binding	GO:0043169	Molecular Function	-
Sma3	carbohydrate metabolic process	GO:0005975	Biological Process	-
Sma3	defense response	GO:0006952	Biological Process	-
Sma3	cellular cell wall organization	GO:0007047	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Glycoside hydrolase, family 17	IPR000490	-	-
Sma3	Phosphopantetheine attachment site	IPR006162	-	-
Sma3	X8	IPR012946	-	-
Sma3	Glycoside hydrolase, subgroup, catalytic domain	IPR013781	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT1G64760.1	O-Glycosyl hydrolases family 17 protein chr1:24054220-24056194 REVERSE LENGTH=481	62%
RefSeq	Arabidopsis thaliana	NP_176656.1	glucan endo-1,3-beta-glucosidase 8 [Arabidopsis thaliana]	62%
RefSeq	Populus trichocarpa	XP_002319699.1	predicted protein [Populus trichocarpa]	62%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NXT6

Fln msg:

Fln protein: MERAWFAILIICALGSMVCAVEGLGVNWGTMALHPLPPDIVVQMLKDNGIKKVKLFDADSNTLQALANTDIEVMVAIPNNMLQHLSDSYKAAEKWVDKNVTRYNFSGGVNIKYVAVGNEPFLSAYNGSYLNNTFPALQNIHKALVKAGLADQIKVTVPLNADVYNSPTDNAVPSAGDFRADIHDLMSQIVTFLNDNGSPFTVNIYPFLSLYADENFPTEFAFFDGDSQPVVDGNIQYTNVFDANLDTLVWSLKKAGVPNMTIIVGEVGWPTDGDKHATAAAAQRFNQGLLKHITSNQGTPMRPGNIEAYLFGLLDEDAKSVAPGNFERHWGIFGYDGQPKYELDLSGPLQNGGLVPAKNVKYLAPKWCVFKTNATDQSKILDSIKYACTYSDCTAMGYGSSCNNLDLYGNASYAFNMYFQVMNQYDINCDFTGLAVITEQNASQGTCKFPIGIAYGAAERSIKIHSILAAVLLGVVTFILSFL*

Protein Length: 485

Fln nts: AATGTTTTCTCACTCAAAATCCATCTGTGAGCTGTTTTAGGCCCCGATCCGAGGCAGCAACGGTCCAGGAACCGTAGAAAGCTTTAAAACGAGCTCTGAAACGCCTCTGGGTGCTCGGATATAGGATTTTATTCAGCAGGAAGAGAAGAAACG_-_ATGGAGCGTGCGTGGTTTGCTATCTTGATTATTTGTGCATTAGGGAGCATGGTCTGTGCAGTGGAAGGGCTGGGGGTGAATTGGGGCACCATGGCATTGCATCCGTTGCCTCCTGATATCGTAGTTCAGATGCTGAAAGACAATGGAATAAAGAAGGTGAAGCTATTTGATGCGGATTCGAACACTCTCCAGGCTTTGGCAAATACTGACATTGAGGTCATGGTGGCTATTCCCAACAATATGTTGCAGCATCTCAGCGACAGTTATAAGGCTGCAGAGAAATGGGTGGACAAGAATGTCACCAGATATAACTTCTCTGGTGGTGTTAACATCAAATATGTTGCAGTTGGAAATGAGCCTTTTCTGTCAGCATACAATGGCTCATATCTGAATAATACATTTCCTGCTCTGCAAAATATTCATAAAGCTCTTGTCAAAGCAGGTTTAGCAGACCAAATAAAAGTTACTGTACCTCTCAATGCAGATGTTTACAATTCCCCAACTGACAATGCAGTACCATCTGCTGGGGATTTTAGAGCAGATATTCATGACCTCATGAGCCAAATTGTGACATTTTTGAATGATAATGGATCACCCTTTACGGTCAATATCTATCCCTTTCTAAGCTTATATGCTGATGAAAACTTCCCCACAGAGTTTGCCTTCTTTGATGGTGATAGTCAGCCAGTAGTTGATGGGAATATCCAGTACACCAATGTCTTTGATGCAAATCTTGATACATTAGTTTGGTCTCTAAAGAAGGCAGGGGTCCCTAATATGACAATCATTGTGGGAGAAGTAGGTTGGCCCACAGATGGTGATAAGCATGCCACTGCTGCAGCTGCTCAGCGGTTCAATCAGGGCCTGTTGAAGCATATAACTAGTAACCAAGGAACACCCATGCGGCCTGGAAATATTGAAGCTTACCTATTTGGCCTTCTTGATGAGGATGCTAAGAGTGTTGCACCTGGTAACTTTGAAAGGCATTGGGGCATTTTTGGGTACGATGGTCAGCCAAAATATGAGCTGGATCTTTCAGGTCCATTGCAGAATGGAGGGTTAGTTCCTGCCAAGAATGTCAAGTATTTAGCTCCAAAGTGGTGTGTGTTCAAGACAAATGCTACAGACCAGTCCAAGATACTTGACAGCATCAAGTATGCTTGTACATATTCAGACTGCACTGCCATGGGTTATGGGTCGTCATGTAACAACTTGGATTTGTATGGCAATGCATCCTATGCATTCAACATGTACTTTCAAGTGATGAATCAATATGATATTAACTGCGATTTTACTGGTCTGGCAGTGATAACAGAGCAAAATGCATCACAGGGAACTTGCAAATTTCCAATAGGGATTGCATATGGTGCAGCAGAGAGGTCCATCAAGATTCATTCTATTCTGGCCGCTGTCCTCTTGGGTGTTGTTACATTTATCCTGTCATTCTTATAG___GAATTTGGATCCGTCAATTCATTCTTTTTATATGCAAATCCTTCTCTTTCCTCCCTTCCTCCCCTTTGACTTAGTCTACAAAGATATGCATTAATTTTTTGTAACCACATACTCAAACTATTGCAATAAAGCGATAGAACCATACCATTATGTATTTGATTCTTAACATTTTATACGAGAAAGTAAGTCATGTTATTGCTACAAAATCAATTTGCCTCGCTGACAAGAATTTTATGGACAAATATTTACTTCAAGCTCACCGTTTTCAGTTAGTGATGTCATTTTGTATGAGCTTAATAGTACCATTTATGTGACTTGATATCAATGAGATCTATTCGTGTGAATTTTG

Fln Alignment:

Contig10552___WFAILIICALGSMVCAVEGLGVNWGTMALHPLPPDIVVQMLKDNGIKKVKLFDADSNTLQALANTDIEVMVAIPNNMLQHLSDSYKAAEKWVDKNVTRYNFSGGVNIKYVAVGNEPFLSAYNGSYLNNTFPALQNIHKALVKAGLADQIKVTVPLNADVYNSPTDNAVPSAGDFRADIHDLMSQIVTFLNDNGSPFTVNIYPFLSLYADENFPTEFAFFDGDSQPVVDGNIQYTNVFDANLDTLVWSLKKAGVPNMTIIVGEVGWPTDGDKHATAAAAQRFNQGLLKHITSNQGTPMRPGNIEAYLFGLLDEDAKSVAPGNFERHWGIFGYDGQPKYELDLSGPLQNGGLVPAKNVKYLAPKWCVFKTNA--TDQSKILDSIKYACTYSDCTAMGYGSSCNNLDLYGNASYAFNMYFQVMNQYDINCDFTGLAVITEQNASQGTCKFPIGIAYGAAERSIK--IHSILAAVLLGVVTFILSFL
A9NXT6_______________WVLVMFV-----LVGWVQGLGVNWGTMATHPLSPDIVVQMLKDNGITKVKLFDAEEHTMKALANTGIQVMVGIPNDQLQMIATSTKDAANWVETNVTEFNLDGSVDIRFVAVGNEPFLESYNGTFIQYTFPALQNIQNALNKAGLSN-IKATVPLNADVY----EGVVPSEGQFRTDINDLMIQICQFLASNGAPFTVNIYPFLSLYSNENFPVDFAFFDGTGQPLIDGNVQYTNVFDAALDTLFWALKKSGFPNLPVLVGEVGWPTDGDKHGNINFAQRFNQGLLKNIMSGLGTPMRFKAAETYLFSLVDEDAKSIAPGNFERHWGIFQYDGTPKYALDLSGQAQNKALVSAKGVKYLPRRWCVINLSASSTALTQLSDSITYACTHGDCTSLGYGSSCNSLNYQGNASYAFNAYYQANNQQNGDCVFSGLAVVTQTDPSQGACKFEIQIAVSATSKAMNSCIYSLISVAGVAAVV-LLSFL

SNPs (tot: 3)

Position	A %	C %	G %	Probability
1731	70		29	1.0
1806	33		66	1.0
1807		88	11	0.923564

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain