SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene9964

Length: 1301 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v3.0_unigene9964 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Malate synthase n=3 Tax=Picea sitchensis RepID=A9NW51_PICSI	-	-	88%
FL-Next	sp=Malate synthase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	88%
Sma3	Malate synthase	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3	Malate synthase.	EC:2.3.3.9	-	0.0	-

Source	KEGGs	Term	Identity
Sma3	Pyruvate metabolism	00620	%
Sma3	Glyoxylate and dicarboxylate metabolism	00630	%
Sma3	Metabolic pathways	01100	%

Source	Gene names
Sma3	At5g03860; CHLREDRAFT_196328; F8F6_70; GSVIVT00017898001; IhMS; LIP; MAS1; MLS; MS; MS1; OJ991113_30.4; OsI_16388; OsJ_15256; PHATRDRAFT_54478; PHYPADRAFT_122530; POPTRDRAFT_776236; RCOM_1506900; THAPSDRAFT_26293;

Source	GOs	Term	Type	Identity
Sma3	glyoxysome	GO:0009514	Cellular Component	-
Sma3	malate synthase activity	GO:0004474	Molecular Function	-
Sma3		GO:0008415	Molecular Function	-
Sma3	lyase activity	GO:0016829	Molecular Function	-
Sma3	glyoxylate cycle	GO:0006097	Biological Process	-
Sma3	tricarboxylic acid cycle	GO:0006099	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Malate synthase	IPR001465	-	-
Sma3	Malate synthase A	IPR006252	-	-
Sma3	Malate synthase-like	IPR011076	-	-
Sma3	Pyruvate/Phosphoenolpyruvate kinase	IPR015813	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G03860.1	MLS malate synthase chr5:1032276-1034527 REVERSE LENGTH=562	73%
RefSeq	Arabidopsis thaliana	NP_001190219.1	malate synthase [Arabidopsis thaliana]	73%
RefSeq	Populus trichocarpa	XP_002322231.1	predicted protein [Populus trichocarpa]	74%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LRM2

Fln msg: your sequence is shorter than subject: 290 - 558

Fln protein: HEILCELGEHSIGLNCGRWDYIFSYVKTFQAHPNRLLPDRVQVGMTQHFMKSYSELLIQTCHKRGVHAMGGMAAQIPIKDNPQANEAASALIQADKLREVKAGHDGTWAAHPGLISLIAEVFDKNMKHPNQIDLKREDVKVTEEDLLEVPKGIRTLEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNWQWIHYEVVLDGEVVPVKVTRELVGRILAEEMARIEREVGTKKFKGGRYEEAAKMFGRQCTAPSLDDFLTLDVYKSILQFHPNPVASSRM*

Protein Length: 291

Fln nts: TGCATGAGATCTTGTGTGAGCTGGGGGAGCACTCAATAGGATTGAACTGTGGCAGATGGGATTACATTTTCAGTTATGTTAAGACATTCCAAGCCCACCCTAACAGGCTTCTTCCGGACCGTGTTCAAGTTGGGATGACACAGCATTTCATGAAAAGCTATTCGGAGCTGCTCATCCAGACATGCCACAAGCGTGGGGTGCATGCAATGGGAGGCATGGCTGCTCAAATACCCATCAAGGATAATCCTCAGGCAAACGAAGCTGCCTCTGCACTTATCCAGGCTGATAAACTACGTGAAGTGAAAGCTGGGCACGACGGAACATGGGCTGCTCATCCTGGTCTGATTTCATTAATAGCAGAGGTGTTTGACAAGAACATGAAACACCCAAATCAAATTGACCTGAAGAGGGAGGATGTGAAGGTTACAGAGGAAGATCTATTGGAGGTGCCTAAAGGGATTCGTACCCTAGAAGGGCTTCGGCTGAATACAAGAGTAGGAATACAGTATCTAGCTGCATGGCTCACAGGCACGGGCTCTGTTCCACTGTACAATTTGATGGAGGACGCGGCCACAGCGGAGATCAGCAGAGTACAAAACTGGCAGTGGATTCATTATGAGGTGGTTCTGGATGGGGAAGTGGTGCCGGTGAAGGTCACCCGGGAATTGGTTGGCAGGATTCTGGCCGAAGAGATGGCGCGGATTGAGAGGGAAGTTGGCACCAAGAAATTCAAGGGCGGGCGATATGAAGAAGCAGCCAAAATGTTTGGAAGGCAGTGCACTGCTCCATCGTTGGATGATTTTCTCACTCTCGATGTCTATAAATCCATCCTGCAATTCCATCCGAACCCAGTCGCATCTTCCCGTATGTGA___TAGTTCCCGCCCTTGAACCAAAGTTTCTCTGCTCTTCTAGTTCTGTTCATGGAAGCTTTGTCGTTTTTTGGGGGCACCAGCAGCATAAATAAATTTCTCCAGGCTCTACCACTCTTTTAGAAAATGTTGAAAAGTTTATAATAAACTTAAATATCTCCAGACTCTACCACTCTTTTGGAAAATGTTGAAAAGTTCATAATAAACTTTTTTAAAAATTAATTATATATACCATAGGGGACAGGGTAACATTTTGAAAAAATAAATTTCAATGGGCAGCAATCTGAAAGGCTATGTCATGGCTTCACATTTATTATTGTAACTATTTTGTTGACCAATAATATAGTGTGTTTTATCTGTGCCGTCTTTGATTATATATGTTTGTGTTTTAAATATTATTTTTCATAATTTTTCATTCTAGGAATGTAATTG

Fln Alignment:

Contig9964___HEILCELGEHSIGLNCGRWDYIFSYVKTFQAHPNRLLPDRVQVGMTQHFMKSYSELLIQTCHKRGVHAMGGMAAQIPIKDNPQANEAASALIQADKLREVKAGHDGTWAAHPGLISLIAEVFDKNMKHPNQIDLKREDVKVTEEDLLEVPKGIRTLEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNWQWIHYEVVLDGEVVPVKVTRELVGRILAEEMARIEREVGTKKFKGGRYEEAAKMFGRQCTAPSLDDFLTLDVYKSILQFHPNPVASSRM
B8LRM2______________HEILYELREHSIGLNCGRWDYIFSYIKTFQAHSNRLVPDRVQVGMTQHFMKSYSELLIQTCHKRGVHAMGGMAAQIPIKDDPQANEAASALVQADKLREVKAGHDGTWAAHPGLISVIADVFDKNMKQPNQINLKREDVKVSEEDLLEVPKGVRTLEGLRLNTRVGIQYLAAWLTGMGSVPLYNLMEDAATVEISRVQNWQWIHYEAILDGEVVPMKVTCELASRILDEEMARIQREVGMEHFKSGRYEEAQRMFGRQCTAQSLDDFLTLDVYKSILQFHSNPVTSSDM

SNPs (tot: 14)

Position	A %	C %	G %	T %	Probability
1020		83		16	0.994512
1022		16		83	0.994512
1064		16		83	0.994512
1065		16		83	0.994512
1214	16		16		0.988813
1216	83	16			0.994512
1274	40	60			1.0
1277	40	60			1.0
1278		60	40		1.0
1279		60		40	1.0
1282	40			20	1.0
1284		50	50		1.0
1288		50		50	1.0
1292	50		50		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain