SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene6856

Length: 2595 nt

UniGene Fasta

>sp_v3.0_unigene6856
CGTCATAAACAGGACATGTTAGACATACACACGAAAGAAAACGAAAACAAAGAGCACTTGGAACGTTCATGCAATTTCCTTAGATTCTCCAACTTATTTAACATTGAATCTCCTTCAGAGCCATTCAGCGGCAGCCAATTCGGCTTCAATGGCGACGGAGGAGCAAATTGAAGGACAATGCGAGGAAATGGACCGTGCCTGCTGCGTGGTTACAGGTGGCCGGGGTTTCGCAGCTCGCCATTTGGTAGTGAAGCTCATCCAATCCGATCTCTGGACTGTAAGAATTGCCGATTTGGCCCCCGAAATTGTATTGGACGACGCCGAGGAGAATGGAACCCTGGGCCAGGCACTGCAATCGGGCCGTGCAGTATATTACAGTGCAGATCTCAGGGATAAGTCACAATTGCTCAAAGTTTGCGAAGGGGCATCTGTTGTCTTCCATATGGCTGCACCAGACTCATCAATCAATAATTTCAATCTTCATTATAGTGTCACAGTCCAAGGTACACGGAATGTACTGAGTGCTTGTATTGAGTGTAAAGTGAAGAAACTTATTTATACTAGCTCCCCAAGTGTTGTATTTGATGGTGTTCATGGAATATATGACGGGGATGAGACCTTACCATATCCTGACAAGCACAATGATATTTATTCTGAGACCAAAGCAGAGGCAGAGGCTTTAGTTTTGCGATCAAATGGAAGAGAAGGACTGCTGACATGTGCCATACGTCCAAGTAGCATATTTGGTCCTGGAGATAAACTTATGGTGCCAGCTGTAGTTGCAGCAGCAAGGGCAGGAAAAAGTAAGTTCATCATTGGTGATGGAGAAAACATGTACGATTTCACTTATGTGGAGAATGTGGCCCATGCACATATATGTGCAGAACATGCATTAGATTCCAACGTGTCTGATGGAAAAGATGCTGCAGCAGGGAAGGCCTATTTTATAACCAATATGGAACCAATAAGGTTCTGGGAATTTATGGCATTGTTACTGGAGGGATTGGGTTATCAGAGGCCCAAAATCCACATTCCTGTGAAAGTGGTGATGCCAATCGGATACATGGTTGAATGGATTTATAAAAAGCTGGGTCCATATGGGATGCCAGTTCCTCAGTTGACACCTTCAAGGATTAGGCTTCTTAGTTGTACCCGAACTTTCAATTGTTCAAGAGCGCAGAAGCTTCTGGGTTACACACCTTTGGTACCTCTCAAGGAAGGGATTAATTTGACAATAGAATCCTATTCACACTTGAGAGCTGAAATGCCTAGTATAAAGAGCCGGAACTTTGATCAGCCATCCAAGGCTTACAAAATGATTGGAAATGAAAGAGTTGCAAATATACTCCTATGGAGAGACGAGAAGCAGACGTTTGCAATAATGCTGGCTTTAATTGCCGTTTTTTATTGTTATTTTACCTCTGGATATACTTTAATGTCAGCCCTGGCCAAGTTGCTCTTGCTGTTCGTTGCGATATTGTTCATGAACAGTATTCTACCTTCATCAATGTTTGGTTATACTAACAGAAGGATTCCACCTCATTATTTTGAAATATCTGAAGAAAGAATGCATTTTGCAACTCAAGCTGTGTCATCTACCTGGAATTCGGGAGTAACTATTTTAAAGATTCTATCCCAAGGAAAGGACTGGGCCTTGTTTCTGAAGACAACGGTTTTGCTCCTTTTTTTCAAGGTTATTGGAGCAATTCCTTTGCCAACCTTATTTGGTGCAGGATTGATTTTCATGTTCAGTGCCTTTTATATATATGAGCAGAAGGAAGAAGAAGTCGATGATCTTGTTAAGGCTTCTATTGTTGTCCTTTCTCAACTGAAGGAGGCAATGTTGAGCAAACTCCCGGGTGTAGATTATCTTACAAAAAAGTAATATTTTTCACAAAGAAAAATGATTGAGCCACCGTATGTTGTCCCTGATATATTCCATATTCTATTGATTTCTGATATTTGAGTGTCCCAACCTTTACCGCTGTTGACTACTGTGGAGCTAGATGTGACCTATGAACCTGGAATACTGTCTTTCTCAATCCCACCAAAAGGTGAAAGCACAGGAATGAATACATGCTTGATTTGGGTGCCTATTGAGAAGTTTATTTTAGGAATTTGAGATAAGATACTCGTAGAATGCACAAGGTTGATTTGTTTGCCTAGGGAAATTTTGAGATTTGAAGTAGCAGTTGACCGGTGGATATTATTTTCAGTTTTCAGATGCTAAGGCAGTTTCAAATGCACCTAGTGAGACTGGAGTTCTGGTTTTCTTGAAGATGTAAGCCTTGTGTTGCTGTCTATCTTTTGGGAAAAGGAGAACAATTCATATAAAACTTTCAAGTAAATAATCGTTCATTGGACAAAGAGATGTTTGCGATTCTATGATATCACTGAGATTCTTGTTCTTGAAGAGATGCTTACGAGACAATATACCTTGTGCAAAATCTTTAGCGTGAATGGCATGTTAGCATGACTTTCACATGTTTTTAGAGATGTTTTATTCCTCTTTAAGAAATTAGTTTGGAGCAATGTTACAAAAGGGAGGTTGCTTAAAAAAATTCTTGAAGCGAAGGTTTTGAGATGTAGTCAAAG

Ace file of the UniGene sp_v3.0_unigene6856

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 [Arabidopsis thaliana] sp\|Q67ZE1.2\|HSDD2_ARATH RecName: Full=3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2; Short=At3BETAHSD/D2; AltName: Full=4alpha-carboxysterol-C3-dehydrogenase/C4	-	-	55%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	91%
Sma3	Putative uncharacterized protein	-	-	-

Source	GOs	Term	Type	Identity
Sma3	endoplasmic reticulum	GO:0005783	Cellular Component	-
Sma3	3-beta-hydroxy-delta5-steroid dehydrogenase activity	GO:0003854	Molecular Function	-
Sma3	binding	GO:0005488	Molecular Function	-
Sma3	steroid biosynthetic process	GO:0006694	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	3-beta hydroxysteroid dehydrogenase/isomerase	IPR002225	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-
Sma3	Reticulon	IPR003388	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT2G26260.1	AT3BETAHSD/D2, 3BETAHSD/D2 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 chr2:11178237-11182872 FORWARD LENGTH=564	57%
RefSeq	Arabidopsis thaliana	NP_180194.2	3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 [Arabidopsis thaliana]	57%
RefSeq	Populus trichocarpa	XP_002308509.1	predicted protein [Populus trichocarpa]	58%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NUL5

Fln msg: Separated hits, possible frame ERROR between 1651 and 1684,

Fln protein: MATEEQIEGQCEEMDRACCVVTGGRGFAARHLVVKLIQSDLWTVRIADLAPEIVLDDAEENGTLGQALQSGRAVYYSADLRDKSQLLKVCEGASVVFHMAAPDSSINNFNLHYSVTVQGTRNVLSACIECKVKKLIYTSSPSVVFDGVHGIYDGDETLPYPDKHNDIYSETKAEAEALVLRSNGREGLLTCAIRPSSIFGPGDKLMVPAVVAAARAGKSKFIIGDGENMYDFTYVENVAHAHICAEHALDSNVSDGKDAAAGKAYFITNMEPIRFWEFMALLLEGLGYQRPKIHIPVKVVMPIGYMVEWIYKKLGPYGMPVPQLTPSRIRLLSCTRTFNCSRAQKLLGYTPLVPLKEGINLTIESYSHLRAEMPSIKSRNFDQPSKAYKMIGNERVANILLWRDEKQTFAIMLALIAVFYCYFTSGYTLMSALAKLLLLFVAILFMNSILPSSMFGYTNRRIPPHYFEISEERMHFATQAVSSTWNSGVTILKILSQGKDxxxxxxxxxxxxFFQGYWSNSFANLIWCRIDFHVQCLLYI*

Protein Length: 542

Fln nts: CGTCATAAACAGGACATGTTAGACATACACACGAAAGAAAACGAAAACAAAGAGCACTTGGAACGTTCATGCAATTTCCTTAGATTCTCCAACTTATTTAACATTGAATCTCCTTCAGAGCCATTCAGCGGCAGCCAATTCGGCTTCA_-_ATGGCGACGGAGGAGCAAATTGAAGGACAATGCGAGGAAATGGACCGTGCCTGCTGCGTGGTTACAGGTGGCCGGGGTTTCGCAGCTCGCCATTTGGTAGTGAAGCTCATCCAATCCGATCTCTGGACTGTAAGAATTGCCGATTTGGCCCCCGAAATTGTATTGGACGACGCCGAGGAGAATGGAACCCTGGGCCAGGCACTGCAATCGGGCCGTGCAGTATATTACAGTGCAGATCTCAGGGATAAGTCACAATTGCTCAAAGTTTGCGAAGGGGCATCTGTTGTCTTCCATATGGCTGCACCAGACTCATCAATCAATAATTTCAATCTTCATTATAGTGTCACAGTCCAAGGTACACGGAATGTACTGAGTGCTTGTATTGAGTGTAAAGTGAAGAAACTTATTTATACTAGCTCCCCAAGTGTTGTATTTGATGGTGTTCATGGAATATATGACGGGGATGAGACCTTACCATATCCTGACAAGCACAATGATATTTATTCTGAGACCAAAGCAGAGGCAGAGGCTTTAGTTTTGCGATCAAATGGAAGAGAAGGACTGCTGACATGTGCCATACGTCCAAGTAGCATATTTGGTCCTGGAGATAAACTTATGGTGCCAGCTGTAGTTGCAGCAGCAAGGGCAGGAAAAAGTAAGTTCATCATTGGTGATGGAGAAAACATGTACGATTTCACTTATGTGGAGAATGTGGCCCATGCACATATATGTGCAGAACATGCATTAGATTCCAACGTGTCTGATGGAAAAGATGCTGCAGCAGGGAAGGCCTATTTTATAACCAATATGGAACCAATAAGGTTCTGGGAATTTATGGCATTGTTACTGGAGGGATTGGGTTATCAGAGGCCCAAAATCCACATTCCTGTGAAAGTGGTGATGCCAATCGGATACATGGTTGAATGGATTTATAAAAAGCTGGGTCCATATGGGATGCCAGTTCCTCAGTTGACACCTTCAAGGATTAGGCTTCTTAGTTGTACCCGAACTTTCAATTGTTCAAGAGCGCAGAAGCTTCTGGGTTACACACCTTTGGTACCTCTCAAGGAAGGGATTAATTTGACAATAGAATCCTATTCACACTTGAGAGCTGAAATGCCTAGTATAAAGAGCCGGAACTTTGATCAGCCATCCAAGGCTTACAAAATGATTGGAAATGAAAGAGTTGCAAATATACTCCTATGGAGAGACGAGAAGCAGACGTTTGCAATAATGCTGGCTTTAATTGCCGTTTTTTATTGTTATTTTACCTCTGGATATACTTTAATGTCAGCCCTGGCCAAGTTGCTCTTGCTGTTCGTTGCGATATTGTTCATGAACAGTATTCTACCTTCATCAATGTTTGGTTATACTAACAGAAGGATTCCACCTCATTATTTTGAAATATCTGAAGAAAGAATGCATTTTGCAACTCAAGCTGTGTCATCTACCTGGAATTCGGGAGTAACTATTTTAAAGATTCTATCCCAAGGAAAGGACTGxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxTTTTTTTCAAGGTTATTGGAGCAATTCCTTTGCCAACCTTATTTGGTGCAGGATTGATTTTCATGTTCAGTGCCTTTTATATATATGA___GCAGAAGGAAGAAGAAGTCGATGATCTTGTTAAGGCTTCTATTGTTGTCCTTTCTCAACTGAAGGAGGCAATGTTGAGCAAACTCCCGGGTGTAGATTATCTTACAAAAAAGTAATATTTTTCACAAAGAAAAATGATTGAGCCACCGTATGTTGTCCCTGATATATTCCATATTCTATTGATTTCTGATATTTGAGTGTCCCAACCTTTACCGCTGTTGACTACTGTGGAGCTAGATGTGACCTATGAACCTGGAATACTGTCTTTCTCAATCCCACCAAAAGGTGAAAGCACAGGAATGAATACATGCTTGATTTGGGTGCCTATTGAGAAGTTTATTTTAGGAATTTGAGATAAGATACTCGTAGAATGCACAAGGTTGATTTGTTTGCCTAGGGAAATTTTGAGATTTGAAGTAGCAGTTGACCGGTGGATATTATTTTCAGTTTTCAGATGCTAAGGCAGTTTCAAATGCACCTAGTGAGACTGGAGTTCTGGTTTTCTTGAAGATGTAAGCCTTGTGTTGCTGTCTATCTTTTGGGAAAAGGAGAACAATTCATATAAAACTTTCAAGTAAATAATCGTTCATTGGACAAAGAGATGTTTGCGATTCTATGATATCACTGAGATTCTTGTTCTTGAAGAGATGCTTACGAGACAATATACCTTGTGCAAAATCTTTAGCGTGAATGGCATGTTAGCATGACTTTCACATGTTTTTAGAGATGTTTTATTCCTCTTTAAGAAATTAGTTTGGAGCAATGTTACAAAAGGGAGGTTGCTTAAAAAAATTCTTGAAGCGAAGGTTTTGAGATGTAGTCAAAG

Fln Alignment:

Contig6856___TEEQIEGQCEEMDRACCVVTGGRGFAARHLVVKLIQSDLWTVRIADLAPEIVLDDAEENGTLGQALQSGRAVYYSADLRDKSQLLKVCEGASVVFHMAAPDSSINNFNLHYSVTVQGTRNVLSACIECKVKKLIYTSSPSVVFDGVHGIYDGDETLPYPDKHNDIYSETKAEAEALVLRSNGREGLLTCAIRPSSIFGPGDKLMVPAVVAAARAGKSKFIIGDGENMYDFTYVENVAHAHICAEHALDSNVSDGKDAAAGKAYFITNMEPIRFWEFMALLLEGLGYQRPKIHIPVKVVMPIGYMVEWIYKKLGPYGMPVPQLTPSRIRLLSCTRTFNCSRAQKLLGYTPLVPLKEGINLTIESYSHLRAEMPSIKSRNFDQPSKAYKMIGNERVANILLWRDEKQTFAIMLALIAVFYCYFTSGYTXXXXXXXXXXXXXXXXFMNSILPSSMFGYTNRRIPPHYFEISEERMHFATQAVSSTWNSGVTILKILSQGKDWxxxxxxxxxxxFFQGYWSNSFANLIWCRIDFHVQCLLYI
A9NUL5______________TEEQVEGQCEELGRGCCVVTGGRGFAARHLVVKLIQSDLWTVRIADLAPKIVLDEAEENGTLGQALQSGRAVYYSADLRDKSQVLIVCEGASVVFHMAAPDSSINNFNLHYSVTVQGTRNVLTACIECKVKKLIYTSSPSVVFDGVHGIYNGDEALRYPDKHNDIYSETKAEAEALVLRSNGREGLLTCAIRPSSIFGPGDKLLVPSLVAAARAGKSKFIIGDGENIYDFTYVENVAHAHICAEQALNSNMADGKDAAAGKAYFITNMEPIKFWEFMSLLLEGLGYQRPKIHIPVKVVMPIAYMVEWIYKKLAPYGMSVPQLTPSRIRLLSCTRTFNCSRAQKLLGYTPLVALEEGINLTIESYSHLRAEIPMIKNRNFDQPSKAYKMIGNERVANILLWRDEKQTFTIMLALIAVFYCYFTSGYTLMSVLAKLLLLFVLILFIHSILPSSMFGYTIRRIPPHYFEVSEERVYFVTQSVSSAWNSGVTILKTLSHGKDWxxxxxxxxxxxFFQVSWINSLANLIWCRIDFHLQCLLYI

SNPs (tot: 26)

Position	A %	C %	G %	T %	Probability
491	25			75	0.996473
508		75		25	0.996473
509	75		25		0.996473
510		75	25		0.996473
613	80		20		0.995493
1092	22		77		0.999999
1479		25		75	0.996473
1572	20	80			0.995493
1575			20	80	0.995493
1742			14	85	0.993533
1743			14	85	0.993533
1744			14	85	0.993533
1811			14	85	0.993533
1814		14		85	0.993533
1819	14			85	0.993533
1824			14	85	0.993533
1827		85	14		0.993533
1828	85			14	0.993533
1832		14	85		0.993533
1919			75	25	0.996473
1986	11	88			0.923564
1989			11	88	0.923564
2233	10		90		0.90298
2236			90	10	0.90298
2364	42			7	1.0
2366	77	11	11		0.968132

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain