SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene6653

Length: 2384 nt

UniGene Fasta

>sp_v3.0_unigene6653
TATGGGGGCAATTTCATGAACGGCTGAACGCACTGGTATTGTTGTAGCAGTTCACACTTTGACACGTCATCGGAGATGTGAAGACTCCAAGGGATCTTTTACCAGTTCTGGCCTACTGTATTGTTTTCCACTAGCGGCTGTTTGTTTTTTTTGAGCAAAGCTTCTCGTTTCAAGAGATGTAATAAATAGCAGTGCAATTAATGTGCAAACTAGGCACTGTCTAGCATCCGCCGGACACACCACATTACATGTTAGAAAGCGAATGAAATATGTAGAGACAGCCAACCATCTGGAACGAGTGTCTAAGGATCTCCTTCCCCGTTGGTATTGAGCAGAACAAAAATCATGGCAGGAGAAGGGAGATCCCTGGCTGAAACTCCAACATGGTCTGTAGCTACAGTTACCACTGTTCTCGTCGCCATCTGTTTTATGGTGGAACGATCCATTCATCGTATTGGAGGGTGGTTGGTAAAAACAAAGCGGAAGCCTCTGCTGGCAGCTCTAGAGAAGATCAAAGAGGAGCTGATGTTACTGGGACTGATATCGTTGATATTGTCTCTATCTTCAAGACGGATGTCTGATATCTGCGTGAAATCATCACTATACAGTAGCCTATTTTATCCTTGTTCCAAGAAAGATTATGAAAGTGACGAAAAACATTTAAACCACTCATCAGTTAGCCGTGTTTACACTCCACAATATCATTTACACGTTGCTGATCAACAACAGCCTGTTGTTAGCAATCCCTGCAATAAGGGTCAGGAGCCTTTTGTTTCATATGAAAGCCTTGAGCAGCTGCATCGCCTTCTCTTTGTCCTTGGTGTTACGCATGTCTTGTATTGCTGTTTGACTATGCTTTTAGCAATGATAAAGATTTATAGCTGGAAGCCCTGGGAAATGGAAGCTCATTCGAAACTTATCACTAATAGGCCTGATGGCACGAGGAGGAATGTGGTATTCAGACGTCAATCTACTTTTGTGCTTAGTCATGCTTCACATCCTTGGATCAAGAATAGAGTCCTTATTTGGGTGATTTGCTTTTTGCGGCAGTTTTTCCGATCTGTAATGAAGGGAGACTACACTGCATTACGTTTGGCCTTCATCACGAACCATAGATTGCGTCTCACCTACGACTTTCATAGCTACATGGTGCGTACTATGGAGGATGAATTCAAAGAAATTGTCGGCATTAGCTGGGAGCTATGGGGTTATGCGATAATATGCATGTTCTTCAATTTGCATGGGCTGAACTTTTACTTCTGGATATCATGGATCCCTGCAATTTTAATATTGTTACTTGGCACAAAACTACAACATGTGATGGCAAGACTGGCCATGGAGAATGTCGTGGATCCAGTTGCAGGGACTCAATTAAAACCTCATGATGCACTTTTCTGGTTTGATAAACCTCAACTCCTGTTGTGGTTTATTCATTTTATTTCATTTCAGAATGCTTTTGAAATGGCGTCATTTATCTGGTCTTGGTGGGAAATTAAAGCTCATACTTGTTTCATGAAGGACCATGCATTTGTCTACACACGTTTAATTTCAGGGCTATTAGTTCAGTTCTGGTGTAGCCATGGCATCCTTCCTCTTTATGCAATTGTTACGCAGATGGGATCCCGGTACAAGAAGGCCTTGGTTACAGAAGAAATCAGAGAATCCCTTCATGGTTGGCGCAAGCGAGTTAGGGAGAAAGCAAAGCGTGATTCCTCACAGCCATCTCTGACAACCACAAAATCATCAATGTCATCTGAGCCTGTTGATGTTGACAAAGATGAAATTGGCAACCGTTTAGAAGCAGAATGTCTCTCTGTCAGTCTGTCTAATATCACAAAAAGGTCCAATAACCTGCAGAGAGAGTTATCTACAACTCCCTTAGCTCAGGATTTTGAGGAGAAAACTACTACTCATCTGATTCAACAAGAGGAAACACTAGGATCTCGTGTTTCTGTATATTCTTCTAGGTCATTAGAATATGTAGTTCCAGTAGAACCAGACAGTATGGATGACATGGATGATGATGATGATTCCACAGATGAGGAAAATGGCGAGGTAGAAGAACCTGTTGTGGAACCCTCTGAAGCTCCTTAAAGTCCTCACATCATGAACCTTCAACTCTTTGGAAGATTGAGAACAAAGGAGAAGAACACTATTTAATGTTACGTTTATAGAAGGATACAGACACAGCTACCATACTTAATAGCCTCCAAGGCCTATGATACAAGGAATGAATGAATGCAATGCAATATCTTTACTTGCACTCTGGCAAATTGGTCCATGGGATCTGTTACATGTAAAGTTGCTCTGTATTGTTCTACAGAGTTTCCTTCTGTTCAAGAATCCCGGTAATGAGAAAAGCTGAATTTTGTTTTCCTTAATTT

Ace file of the UniGene sp_v3.0_unigene6653

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Calmodulin binding protein, putative n=1 Tax=Ricinus communis RepID=B9SXF3_RICCO	-	-	0.0	50%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	40%
Sma3	MLO-like protein 4	-	-	1.563e-08	-

Source	Gene names
Sma3	AT4G02600; AT5G53760; At1g11000; At1g26700; At2g17430; At4g02600; At5g53760; F5J6.19; GSVIVT00027647001; GSVIVT00035478001; GSVIVT00036763001; H0901F07.8; LOC_Os11g07920; MLO-H1; MLO1; MLO11; MLO14; MLO4; MLO7; OSJNBb0017I01.22; Os04g0680800; Os11g0181400

Source	GOs	Term	Type	Identity
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	integral to membrane	GO:0016021	Cellular Component	-
Sma3	iron ion binding	GO:0005506	Molecular Function	-
Sma3	calmodulin binding	GO:0005516	Molecular Function	-
Sma3	oxidoreductase activity	GO:0016491	Molecular Function	-
Sma3	iron-sulfur cluster binding	GO:0051536	Molecular Function	-
Sma3	defense response	GO:0006952	Biological Process	-
Sma3	cell death	GO:0008219	Biological Process	-
Sma3	response to biotic stimulus	GO:0009607	Biological Process	-
Sma3	iron-sulfur cluster assembly	GO:0016226	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	NIF system FeS cluster assembly, NifU, C-terminal	IPR001075	-	-
Sma3	Short-chain dehydrogenase/reductase SDR	IPR002198	-	-
Sma3	Glucose/ribitol dehydrogenase	IPR002347	-	-
Sma3	Transcription factor CBF/NF-Y/archaeal histone	IPR003958	-	-
Sma3	Mlo-related protein	IPR004326	-	-
Sma3	Histone-fold	IPR009072	-	-
Sma3		IPR015706	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT1G11000.1	MLO4, ATMLO4 Seven transmembrane MLO family protein chr1:3671935-3675909 REVERSE LENGTH=573	58%
RefSeq	Arabidopsis thaliana	NP_563882.1	MLO-like protein 4 [Arabidopsis thaliana]	58%
RefSeq	Populus trichocarpa	XP_002331489.1	predicted protein [Populus trichocarpa]	52%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LMC0

Fln msg: your sequence is longer than subject: 583 - 483

Fln protein: MAGEGRSLAETPTWSVATVTTVLVAICFMVERSIHRIGGWLVKTKRKPLLAALEKIKEELMLLGLISLILSLSSRRMSDICVKSSLYSSLFYPCSKKDYESDEKHLNHSSVSRVYTPQYHLHVADQQQPVVSNPCNKGQEPFVSYESLEQLHRLLFVLGVTHVLYCCLTMLLAMIKIYSWKPWEMEAHSKLITNRPDGTRRNVVFRRQSTFVLSHASHPWIKNRVLIWVICFLRQFFRSVMKGDYTALRLAFITNHRLRLTYDFHSYMVRTMEDEFKEIVGISWELWGYAIICMFFNLHGLNFYFWISWIPAILILLLGTKLQHVMARLAMENVVDPVAGTQLKPHDALFWFDKPQLLLWFIHFISFQNAFEMASFIWSWWEIKAHTCFMKDHAFVYTRLISGLLVQFWCSHGILPLYAIVTQMGSRYKKALVTEEIRESLHGWRKRVREKAKRDSSQPSLTTTKSSMSSEPVDVDKDEIGNRLEAECLSVSLSNITKRSNNLQRELSTTPLAQDFEEKTTTHLIQQEETLGSRVSVYSSRSLEYVVPVEPDSMDDMDDDDDSTDEENGEVEEPVVEPSEAP*

Protein Length: 584

Fln nts: TATGGGGGCAATTTCATGAACGGCTGAACGCACTGGTATTGTTGTAGCAGTTCACACTTTGACACGTCATCGGAGATGTGAAGACTCCAAGGGATCTTTTACCAGTTCTGGCCTACTGTATTGTTTTCCACTAGCGGCTGTTTGTTTTTTTTGAGCAAAGCTTCTCGTTTCAAGAGATGTAATAAATAGCAGTGCAATTAATGTGCAAACTAGGCACTGTCTAGCATCCGCCGGACACACCACATTACATGTTAGAAAGCGAATGAAATATGTAGAGACAGCCAACCATCTGGAACGAGTGTCTAAGGATCTCCTTCCCCGTTGGTATTGAGCAGAACAAAAATC_-_ATGGCAGGAGAAGGGAGATCCCTGGCTGAAACTCCAACATGGTCTGTAGCTACAGTTACCACTGTTCTCGTCGCCATCTGTTTTATGGTGGAACGATCCATTCATCGTATTGGAGGGTGGTTGGTAAAAACAAAGCGGAAGCCTCTGCTGGCAGCTCTAGAGAAGATCAAAGAGGAGCTGATGTTACTGGGACTGATATCGTTGATATTGTCTCTATCTTCAAGACGGATGTCTGATATCTGCGTGAAATCATCACTATACAGTAGCCTATTTTATCCTTGTTCCAAGAAAGATTATGAAAGTGACGAAAAACATTTAAACCACTCATCAGTTAGCCGTGTTTACACTCCACAATATCATTTACACGTTGCTGATCAACAACAGCCTGTTGTTAGCAATCCCTGCAATAAGGGTCAGGAGCCTTTTGTTTCATATGAAAGCCTTGAGCAGCTGCATCGCCTTCTCTTTGTCCTTGGTGTTACGCATGTCTTGTATTGCTGTTTGACTATGCTTTTAGCAATGATAAAGATTTATAGCTGGAAGCCCTGGGAAATGGAAGCTCATTCGAAACTTATCACTAATAGGCCTGATGGCACGAGGAGGAATGTGGTATTCAGACGTCAATCTACTTTTGTGCTTAGTCATGCTTCACATCCTTGGATCAAGAATAGAGTCCTTATTTGGGTGATTTGCTTTTTGCGGCAGTTTTTCCGATCTGTAATGAAGGGAGACTACACTGCATTACGTTTGGCCTTCATCACGAACCATAGATTGCGTCTCACCTACGACTTTCATAGCTACATGGTGCGTACTATGGAGGATGAATTCAAAGAAATTGTCGGCATTAGCTGGGAGCTATGGGGTTATGCGATAATATGCATGTTCTTCAATTTGCATGGGCTGAACTTTTACTTCTGGATATCATGGATCCCTGCAATTTTAATATTGTTACTTGGCACAAAACTACAACATGTGATGGCAAGACTGGCCATGGAGAATGTCGTGGATCCAGTTGCAGGGACTCAATTAAAACCTCATGATGCACTTTTCTGGTTTGATAAACCTCAACTCCTGTTGTGGTTTATTCATTTTATTTCATTTCAGAATGCTTTTGAAATGGCGTCATTTATCTGGTCTTGGTGGGAAATTAAAGCTCATACTTGTTTCATGAAGGACCATGCATTTGTCTACACACGTTTAATTTCAGGGCTATTAGTTCAGTTCTGGTGTAGCCATGGCATCCTTCCTCTTTATGCAATTGTTACGCAGATGGGATCCCGGTACAAGAAGGCCTTGGTTACAGAAGAAATCAGAGAATCCCTTCATGGTTGGCGCAAGCGAGTTAGGGAGAAAGCAAAGCGTGATTCCTCACAGCCATCTCTGACAACCACAAAATCATCAATGTCATCTGAGCCTGTTGATGTTGACAAAGATGAAATTGGCAACCGTTTAGAAGCAGAATGTCTCTCTGTCAGTCTGTCTAATATCACAAAAAGGTCCAATAACCTGCAGAGAGAGTTATCTACAACTCCCTTAGCTCAGGATTTTGAGGAGAAAACTACTACTCATCTGATTCAACAAGAGGAAACACTAGGATCTCGTGTTTCTGTATATTCTTCTAGGTCATTAGAATATGTAGTTCCAGTAGAACCAGACAGTATGGATGACATGGATGATGATGATGATTCCACAGATGAGGAAAATGGCGAGGTAGAAGAACCTGTTGTGGAACCCTCTGAAGCTCCTTAA___AGTCCTCACATCATGAACCTTCAACTCTTTGGAAGATTGAGAACAAAGGAGAAGAACACTATTTAATGTTACGTTTATAGAAGGATACAGACACAGCTACCATACTTAATAGCCTCCAAGGCCTATGATACAAGGAATGAATGAATGCAATGCAATATCTTTACTTGCACTCTGGCAAATTGGTCCATGGGATCTGTTACATGTAAAGTTGCTCTGTATTGTTCTACAGAGTTTCCTTCTGTTCAAGAATCCCGGTAATGAGAAAAGCTGAATTTTGTTTTCCTTAATTT

Fln Alignment:

Contig6653___SLAETPTWSVATVTTVLVAICFMVERSIHRIGGWLVKTKRKPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDICVKSSLYSSLFYPCSKKDYES--DEKHLNHSSVSRVYTPQYHLHVADQQQPVVSNPCNKGQEPFVSYESLEQLHRLLFVLGVTHVLYCCLTMLLAMIKIYSWKPWEMEAHSKLITNRPDGTRRNVVFRRQSTFVLSHASHPWIKNRVLIWVICFLRQFFRSVMKGDYTALRLAFITNH-RLRLTYDFHSYMVRTMEDEFKEIVGISWELWGYAIICMFFNLHGLNFYFWISWIPAILILLLGTKLQHVMARLAMENVVDPVA---GTQLKPHDALFWFDKPQLLLWFIHFISFQNAFEMASFIWSWWEIKAHTCFMKDHAFVYTRLISGLLVQFWCSHGILPLYAIVTQMGSRYKKALVTEEIRESLHGWRKRVREKAK-RDSSQPSLTTTKSSM
B8LMC0______________SLKYTPTWTVAVVCTIFVGISLCLERGIHRLGKTLRRRNQNPLYEALEKLKEELMLLGFISLLLTAFQGAINRLCLPES-FTNHMLPCKRPESAEGPSSEAVNHSRELIAFVGSNTRRLLSSGGSEGSH-CSEGKKPFLSLEAVHQLHIFIFVLAIVHVLFSVLTMLLGSAKIRTWKPWEEDIQAKRYGTEDAINRITQVAHHD--FVKTHAMGTWGKSDLLSWMVSFLRQFYGSVKKSDYSTLRLGFIEKHCKTNPTFNFHNYMMRTLEADFKKVVGISSYLWGFVVIFLLLNIEGWHTYFWIAFIPLILLLAVGAKLEHIITQLAQEVVEKHTAVEGDLVVRLSDEHFWFGKPKLVLYLIHFILFQNAFEIGFFFWILTTYGFDSCIMGKIAYIIPRLAVGVIIQVLCSYSTLPLYVLVTQMGTSFKREILNEHIHEGLSVWHQKAKAKAKGRSTSKPNDDDNKGSI

SNPs (tot: 48)

Position	A %	C %	G %	T %	Probability
150			33	66	1.0
291	28	71			1.0
296	71		28		1.0
297		71	28		1.0
344	75		25		0.996473
359	20		80		0.995493
360			80	20	0.995493
363			80	20	0.995493
608	87		12		0.992554
609	12		87		0.992554
630	22	77			0.999999
631	11	88			0.923564
633	88		11		0.923564
634		11	88		0.923564
635	77	11	11		0.968132
652		71		28	1.0
714	12			87	0.992554
715		12		87	0.992554
716	12		87		0.992554
717		87		12	0.992554
856			70	30	1.0
857		90		10	0.90298
858		10		90	0.90298
966	11		77		0.968132
967	11			88	0.923564
968		88		11	0.923564
970	87			12	0.992554
1067	83		16		0.994512
1138		50		50	1.0
1462	80		20		0.995493
1519			90	10	0.90298
1522		90		10	0.90298
1747	25	75			0.996473
1783	66		33		0.997452
1838	83			16	0.994512
1839	83		16		0.994512
1840	83		16		0.994512
1841	83		16		0.994512
1876	14			85	0.993533
1877		85		14	0.993533
1878		85	14		0.993533
1879		85	14		0.993533
1880			16	83	0.994512
2038	88			11	0.999937
2077	82		17		1.0
2216	26		73		1.0
2288		25		75	1.0
2342			75	25	1.0

SSRs (tot: 2)

Start position	End position	Sequence	Length
2017	2031	GAT GAT GAT GAT GAT	15
2229	2241	GAAT GAAT GAAT G	13

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain