SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene6546

Length: 1857 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene6546

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	[H] COG0108 3,4-dihydroxy-2-butanone 4-phosphate synthase	-	-	0.0	53%
FL-Next	sp=Riboflavin biosynthesis protein ribBA, chloroplastic; Arabidopsis thaliana (Mouse-ear cress).	-	-	0.0	63%
Sma3	GTP cyclohydrolase II, putative	-	-	9.815e-20	-

Source	ECs	Term	Type	e value	Identity
Sma3	GTP cyclohydrolase II.	EC:3.5.4.25	-	9.815e-20	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Riboflavin metabolism	00740		9.815e-20	%
Sma3	Metabolic pathways	01100		9.815e-20	%

Source	Gene names
Sma3	At2g22450; At5g59750; At5g64300; CHLREDRAFT_102099; GSVIVT00010047001; GSVIVT00012102001; GSVIVT00012169001; GSVIVT00024399001; GSVIVT00031262001; MICPUCDRAFT_33832; MICPUCDRAFT_45490; MICPUN_59692; MSJ1.14; OJ1111_H02.5; OJ1281_H05.8; OSJNBb0092C08.34; O

Source	GOs	Term	Type	Identity
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	membrane	GO:0016020	Cellular Component	-
Sma3	magnesium ion binding	GO:0000287	Molecular Function	-
Sma3	GTP cyclohydrolase II activity	GO:0003935	Molecular Function	-
Sma3	GTP binding	GO:0005525	Molecular Function	-
Sma3	zinc ion binding	GO:0008270	Molecular Function	-
Sma3	3,4-dihydroxy-2-butanone-4-phosphate synthase activity	GO:0008686	Molecular Function	-
Sma3	manganese ion binding	GO:0030145	Molecular Function	-
Sma3	riboflavin biosynthetic process	GO:0009231	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	3,4-dihydroxy-2-butanone 4-phosphate synthase, RibB	IPR000422	-	-
Sma3	GTP cyclohydrolase II, RibA	IPR000926	-	-
Sma3	Inorganic pyrophosphatase	IPR008162	-	-
Sma3	TonB box, conserved site	IPR010916	-	-
Sma3	Riboflavin biosynthesis protein RibBA	IPR016299	-	-
Sma3	DHBP synthase RibB-like alpha/beta domain	IPR017945	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G64300.1	ATGCH, GCH, ATRIBA1, RFD1 GTP cyclohydrolase II chr5:25718459-25720790 FORWARD LENGTH=543	63%
RefSeq	Arabidopsis thaliana	NP_568913.1	GTP cyclohydrolase II [Arabidopsis thaliana]	69%
RefSeq	Populus trichocarpa	XP_002299894.1	predicted protein, partial [Populus trichocarpa]	72%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: sp_plants

Fln subject: P47924

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 63 aas, putative chimeric sequence, your sequence is shorter than subject: 471 - 543

Fln protein: MDMVLRSTPLHIHSTPLHGYKSSGKSSLALYKRLHSSVVAKRKTWFPRVGFALNIMKEDNESFKNSSSEDDIIIPIGVHADKKGNSAGFHTVSAEITPVTDEFFVSDTEGDPDCPSEGFSSIEQALNAIRQGKFVIAVDDEDRENTGDIIMAASSVTAQAMAFMVKHGTGIVCVGMMEEDLERLRLPLMVPKKENEDRSITAFTVSVDAKVGTSTGVSATDRANTILALASPKSQAEDFRRPGHIFPLKYRQGGVLKRTGHTEAAVDLVKLAGLRPVSVLSVIIDDDGSVACLPKLQKFAAEYNVPIISIADLVRYRRKREKLVEKTAVSCLPTKWGLFQVYCYRSKLDGIEHVAMVKGNIGDGQDVIVRVHSECLTGDIFGSARCDCGNQIALAMQEINRVGRGVLVYLRGHEGRGIGLGHKLHAYNLQDKGHDTVEANLELGLSIDSREYGIGAQVKNLMTYTIQYMQ*

Protein Length: 472

Fln nts: CGTCGTGACCTTGGCTGTCACTCAAATTCTCAGAGCAG_-_ATGGATATGGTTCTACGGTCCACTCCTCTACACATACACTCAACTCCTTTACACGGATACAAATCGAGTGGGAAGAGCTCACTTGCTCTATATAAACGATTGCATTCATCGGTGGTTGCCAAGAGGAAAACATGGTTTCCAAGAGTGGGTTTTGCGTTGAATATCATGAAAGAAGATAATGAGTCCTTCAAAAACTCCAGCTCAGAAGATGATATTATTATACCAATTGGAGTTCATGCTGATAAAAAGGGGAATTCTGCAGGATTTCATACAGTTTCGGCAGAAATAACCCCTGTTACTGATGAGTTTTTTGTCAGTGACACCGAGGGGGACCCAGATTGCCCCTCCGAAGGATTTTCTTCTATTGAGCAAGCACTTAATGCAATTCGTCAAGGCAAGTTTGTGATTGCTGTGGATGATGAAGACAGGGAAAACACGGGAGACATTATCATGGCAGCATCTTCTGTAACTGCTCAAGCAATGGCATTTATGGTTAAACACGGCACGGGGATAGTGTGTGTTGGCATGATGGAAGAAGACTTAGAGAGATTGAGACTTCCACTAATGGTGCCCAAGAAGGAAAATGAGGATAGATCTATCACAGCTTTCACTGTATCTGTTGATGCCAAAGTTGGCACCTCTACAGGTGTTTCAGCAACAGACAGAGCAAATACAATTCTAGCTCTTGCATCTCCAAAATCTCAGGCGGAGGACTTCAGGCGGCCTGGTCATATCTTTCCTTTGAAATATAGACAGGGTGGTGTCTTAAAGAGAACTGGTCATACGGAAGCTGCAGTGGATTTGGTAAAATTGGCAGGACTGAGGCCTGTCAGTGTGCTTTCTGTGATTATTGATGATGACGGTTCAGTTGCATGCTTACCAAAGTTACAGAAATTTGCTGCAGAATACAATGTACCAATCATTTCCATTGCAGATCTCGTAAGGTACAGGAGAAAACGAGAAAAGTTGGTGGAAAAAACCGCTGTTTCATGTTTGCCAACAAAGTGGGGCCTCTTTCAAGTTTATTGTTATCGTTCAAAGCTCGATGGCATTGAACATGTTGCAATGGTAAAGGGAAATATTGGAGATGGGCAAGATGTTATAGTGAGAGTGCATTCAGAATGTCTAACTGGTGATATCTTTGGATCTGCTCGATGTGATTGTGGAAATCAAATTGCATTAGCAATGCAAGAAATCAACCGAGTTGGTAGAGGAGTATTGGTGTACTTGAGAGGTCATGAAGGCAGGGGAATTGGGTTGGGTCATAAACTCCACGCATACAATCTCCAAGATAAAGGTCATGATACTGTAGAAGCCAATTTGGAACTTGGGTTATCAATTGATTCTCGTGAATATGGTATTGGTGCTCAGGTAAAGAACTTAATGACATATACTATACAATACATGCAGTAA___CAAGGCTTGCATCTCTGTGATCAGTTTGCTCAACTTGATTGCAATAGGAATCCTTGGTGAACTGACCAAGTCATGAAGCATTTTCTTGACATCATGGAGTTGGAGACCCTAGTTTTAATTGGTCTTCTCAGTGTTGCAAACATCCTTGATTTCTCCCAATATTTATGATTTTTTCCTAACTTTCCAGGTTACTAATATTTTTGGGTTTTTTATGTCCAAGTTTATGCTTGCCTATATTTTCTCTTATATTTTCAGTAAATTTCTTTCTAGATCCTCAGTGATATATTCCCAGAAATAATATTTGCAACATTGGGTCTTCTTCACTGGCTACTATTCTTGACTGTAGAACCGAGAGATGGATATGACATCTTTGATTTAATAAGAAATATAGTGGAATTAATTCTTG

Fln Alignment:

Contig6546___KTWFPRVGFALNIMKEDNESFKNSSS--EDDIIIPIGVHADKKGNSAGFHTVSAEITPVTDEFFVS-DTEGDPDCPSEGFSSIEQALNAIRQGKFVIAVDDEDRENTGDIIMAASSVTAQAMAFMVKHGTGIVCVGMMEEDLERLRLPLMVPKKENEDRSITAFTVSVDAKVGTSTGVSATDRANTILALASPKSQAEDFRRPGHIFPLKYRQGGVLKRTGHTEAAVDLVKLAGLRPVSVLSVIIDDDGSVACLPKLQKFAAEYNVPIISIADLVRYRRKREKLVEKTAVSCLPTKWGLFQVYCYRSKLDGIEHVAMVKGNIGDGQDVIVRVHSECLTGDIFGSARCDCGNQIALAMQEINRVGRGVLVYLRGHEGRGIGLGHKLHAYNLQDKGHDTVEANLELGLSIDSREYGIGAQ-VKNLMTYTIQYM
P47924______________KTNTGKVKAAVISREDDLLSFTNGNTPLSNGSLIDDRTEEPLEADSVSLGTLAADSAPAPANGFVAEDDDFELDLPTPGFSSIPEAIEDIRQGKLVVVVDDEDRENEGDLVMAAQLATPEAMAFIVRHGTGIVCVSMKEDDLERLHLPLMVNQKENEEKLSTAFTVTVDAKHGTTTGVSARDRATTILSLASRDSKPEDFNRPGHIFPLKYREGGVLKRAGHTEASVDLTVLAGLDPVGVLCEIVDDDGSMARLPKLREFAAENNLKVVSIADLIRYRRKRDKLVERASAARIPTMWGPFTAYCYRSILDGIEHIAMVKGEIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALSMQQIEATGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEELGLPVDSREYGIGAQIIRDLGVRTMKLM

SNPs (tot: 5)

Position	A %	C %	G %	T %	Probability
1150	80		20		0.995493
1279			25	75	0.996473
1416	90		10		0.90298
1734	83			16	0.994512
1816		11		88	0.991528

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain