SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene5470

Length: 1564 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene5470

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Fructokinase-like protein 1 n=1 Tax=Solanum tuberosum RepID=D9IWP2_SOLTU	-	-	0.0	66%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	34%
Sma3	Putative fructokinase	-	-	2.843e-08	-

Source	ECs	Term	Type	e value	Identity
Sma3	Fructokinase.	EC:2.7.1.4	-	4.397e-07	-

Source	KEGGs	Term	e value	Identity
Sma3	Fructose and mannose metabolism	00051	4.397e-07	%
Sma3	Starch and sucrose metabolism	00500	4.397e-07	%
Sma3	Amino sugar and nucleotide sugar metabolism	00520	4.397e-07	%
Sma3	Metabolic pathways	01100	4.397e-07	%

Source	Gene names
Sma3	At1g69200; At3g54090; F23O10.21; F24B22.50; GSVIVT00021445001; GSVIVT00030019001; LOC_Os03g40550; OJ1519_A12.11; Os01g0851000; Os03g0602600; OsI_04455; OsI_12536; OsJ_04093; OsJ_11670; P0529H11.26; PHYPADRAFT_141786; POPTRDRAFT_1104137; POPTRDRAFT_872310;

Source	GOs	Term	Type	Identity
Sma3	nucleus	GO:0005634	Cellular Component	-
Sma3	nucleoid	GO:0009295	Cellular Component	-
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	kinase activity	GO:0016301	Molecular Function	-

Source	InterPros	Term	Type	e value	Identity
Sma3	Carbohydrate/puine kinase, PfkB, conserved site	IPR002173	-	0.0	-
Sma3	Carbohydrate/purine kinase	IPR011611	-	0.0	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT3G54090.1	FLN1 fructokinase-like 1 chr3:20028145-20029835 FORWARD LENGTH=471	63%
RefSeq	Arabidopsis thaliana	NP_190977.1	fructokinase-like 1 [Arabidopsis thaliana]	63%
RefSeq	Populus trichocarpa	XP_002322950.1	predicted protein [Populus trichocarpa]	62%

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LNK6

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 55 aas, putative chimeric sequence, your sequence is shorter than subject: 337 - 408

Fln protein: MELEADGEEDDHNFEYDWPPLVCCFGLAQNEFIPTVRVSDRQMDEDKYSTWKGLQWSPPEFVRAPGGPPSNVAIALARLGGRVAFMGKVGDDPTGYQMVLTMNKEHVQTRGVKFDPYASTAVSYMKVTRREGKLGMECVKPCAEDGFHISDINIDILKEARMFHFNSMVLLEQSMHATLLAAIKMSKEYGGVIFFDVNLPLPLWRSRDETRKLIQSAWNEANVIEVTMQELEFLIDEDYFERKRNYRPQYYAHSYEQSVGERKRQRRRRCYHYTREEIAPLWHDNIKILFVTDGTFRIHYYTPTFEGAVIGTEDVLVTPFTCDRTGSGDAIVAGSH*

Protein Length: 338

Fln nts: GTATGGAGTTGGAAGCAGATGGTGAGGAGGATGATCATAACTTTGAATATGATTGGCCTCCTTTGGTATGCTGTTTTGGCTTGGCGCAGAATGAGTTTATTCCTACTGTTAGGGTATCAGACAGACAAATGGATGAAGATAAGTATTCTACATGGAAGGGCTTGCAATGGAGTCCTCCAGAATTTGTCAGGGCACCGGGAGGACCACCATCCAATGTTGCTATTGCACTTGCAAGGTTGGGAGGTAGAGTAGCATTTATGGGTAAAGTAGGTGATGATCCCACTGGTTATCAAATGGTTCTCACGATGAACAAGGAACATGTCCAAACTCGTGGTGTTAAATTTGATCCTTATGCATCCACAGCAGTTTCCTACATGAAGGTAACCCGTCGGGAAGGAAAATTAGGAATGGAATGTGTCAAACCATGTGCAGAAGATGGTTTTCACATTTCTGATATTAACATCGACATACTGAAAGAGGCCAGGATGTTTCATTTCAATTCCATGGTTCTTCTTGAACAATCCATGCATGCCACTCTTCTTGCTGCAATCAAAATGTCAAAAGAATATGGGGGAGTTATATTTTTTGATGTAAATCTCCCTTTGCCACTTTGGAGGTCTCGAGATGAGACAAGGAAACTTATCCAGAGTGCATGGAATGAGGCAAATGTCATTGAGGTAACCATGCAAGAACTGGAATTTTTGATTGATGAGGATTATTTTGAGCGAAAGAGGAATTACAGGCCTCAATACTATGCACATTCCTATGAACAGTCAGTCGGAGAAAGAAAAAGGCAACGACGACGGAGGTGTTATCATTACACAAGAGAGGAAATTGCGCCGTTATGGCATGATAACATAAAGATTCTGTTTGTCACTGATGGCACCTTCCGCATACACTACTACACACCTACATTTGAGGGTGCAGTAATAGGAACAGAAGATGTTTTGGTCACGCCATTCACATGTGATAGGACTGGTTCGGGTGATGCTATTGTAGCAGGTAGTCATTGA___ATGCAGTCACATTGCTCAAGGATCTATCTGAAGAGTTCTTATACTTCTAATAGAAGGATGATATGTCGATGCAGAGTTCAACATTGTTAGTAACTGTGTATGTCTTGCAGGCATTATCAGAAAGTTGACTTCACAACCGGAGCTATATGATGATCAAATGCTGTTGGAGAAGCAATTAAGATTTGCTATTTGTGCTGGGATTATTGCACAATGGACAATTGGTGCTGTCCGGGGATTCCCAACTGAAAGCGCAACACAGAATCTGAAGGAGCAAGTATATGTACCTTCCTTGTGGTAGGCTATTTAGAGGTTTAGCATAGCTCCATTGATGCTGCAAGGTAATGTATTATTGTATTAAGGTACCTTTTGTCTCGCTGGACACTACATCATGAACTTTGCCTTTCAGGTTCTTCTCATGGGCATTGCTTTTGAGGATTCGATATCCAGCTGTGACACATATTTTAAGAGACAAATTTATGGATTTTCACAATTCCTGTCAATTCCCTCTGTACCCTTTTTCATTTTAAGATGTTTGATATGATAAGTACATT

Fln Alignment:

Contig5470___EADGEEDDHNFEYDWPPLVCCFGLAQNEFIPTVRVSDRQMDEDKYSTWKGLQWSP-PEFVRAPGGPPSNVAIALARLGGRVAFMGKVGDDPTGYQMVLTMNKEHVQTRGVKFDPYASTAVSYMKVTRREGKLGMECVKPCAEDGFHISDINIDILKEARMFHFNSMVLLEQSMHATLLAAIKMSKEYGGVIFFDVNLPLPLWRSRDETRKLIQSAWNEANVIEVTMQELEFLIDEDYFERKRNYRPQYYAHSYEQSVGEXXXXXXXXCYHYTREEIAPLWHDNIKILFVTDGTFRIHYYTPTFEGAVIGTEDVLVTPFTCDRTGSGDAIVAG
B8LNK6______________KALSESHDNGKPSEVSPLVVCFGEMLIDFVPTV---------------SGVSLAKAPAFKKAPGGAPANVAVGIARLGGSSAFMGKVGDDEFGYMLADIMKENNVEHRGMRFDPVARTALAFVTLKADGEREFMFFRNPSADMLLTESELEVDLIQQAKIFHYGSISLITEPCRSAHLAAMRIAKDTGTLLSYDPNLRLPLWSSASAAREGILSIWDEADVIKVSDEEVEFLTEG--------------ADPYDDTVARN------------------LFHPNLKLLLVTEGDQGCRYYTKDFSGRVNG-----VAVQAVDTTGAGDAFVSG

SNPs (tot: 13)

Position	A %	C %	G %	T %	Probability
967	66		33		1.0
1071	30		70		1.0
1119		83	16		0.999991
1121	16			83	0.999991
1131	90		10		0.90298
1132			10	90	0.90298
1134	90			10	0.90298
1136	90			10	0.90298
1137	90	10			0.90298
1139		10	90		0.90298
1141	10			90	0.90298
1166		14		85	0.993533
1205		20		80	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain