SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene5465

Length: 2433 nt

UniGene Fasta

>sp_v3.0_unigene5465
AATGTGTAAAAGATGATGACGTCCGTCTGAAGGAGACAGGCCGCTGGGATGGCCAAAGCGACGATGATTCGGAGAGCGATGATAATGATAAACCCTCTCCTGCAAAAGCTCCCAAAAAAACCCGCAAGGAGAATAATGGGACGAATGACACAGAAATGGCAAGTGATAAGAAAGTCTCTACTGGATTTTTTGCATCAGCAGAAGGGGCTTCTTTTAGTGCAAATTCCTTTGTTGAATTAAATCTTTCACGCCCATTGCTGAGGGCATGTGAATCCCTTGGTTATAAAAAGCCAACTCCGATTCAGGCAGCATGTATACCTTTGGCATTAACTGGACGCGATATATGTGGAAGTGCTATTACTGGATCTGGAAAGACAGCTGCATTTGCTCTACCAGTGTTGGAGAGATTGCTTTACCGTCCTAGACGCATCCCGGCCATAAGAGTCCTTATTCTCACTCCAACGAGGGAACTTGCTGTTCAGGTACACAGTATGACAGACAAGATTGCACAGTTTACAGATATCCGCAGTTGCCTTGTTGTTGGGGGTCTGTCAACTAAGGCACAGGAATCAGCATTAAGGTCCCGGCCTGATATTGTTGTAGCAACGCCAGGGAGGATGATAGATCATTTGCACAATGCACAGTCAGTTGGACTGGAAGAACTTGCAGTATTGATTCTTGACGAGGCTGACCGTCTTCTGGAGCTTGGTTTTAGCAGTGAAGTTCAAGAGCTGGTTAAACTTTGTCCAAAACGAAGACAAACGATGTTGTTCTCAGCTACAATGACAGAGGAAGTGGATGAACTTGTCAAGCTTTCACTTACTAGTCCTGTGCGTCTTTCAGCAGATCCGTCGACAAAGAGGCCATCAACACTGACTGAAGAGGTAATAAAGATTCGACCTGTACATGAAGCCAAGCAGGAAGCAATATTGCTTGCATTATGTTCCCGAACCTTTACAAGCAAAGCAATTATCTTCAGTGGCACCAAACAAGCAGCACATCGCTTGAAAATCTTATTCGGACTCTCAGATTTGAAAGCTGCTGAGCTGCATGGAAATCTCACTCAAGCACAACGGCTTGATGCCCTTGAACAATTCAGAAAACAAGAAGTTGATTTTCTTATTGCGACTGATGTAGCAGCTCGGGGCCTGGACATCATCGGTGTTAAAACAGTAATAAACTTTGCATGTCCTCGTGATATCACATCCTATGTTCATAGAGTTGGTAGAACGGCAAGAGCTGGTAAGGAAGGCTGTGCCATCACTTTTGTTACAGAACGGGATCGCCGTCTTTTGAAAGCTATGGCAAAAAGAGCTGGCTCTAAGCTCCTGAGCAGGGTAGTTGCAGAACAGTCCATTGCTAAGTGGTGTCAGAATATTGAACAAATGGAGGATGATGTTGCTTCAATCATTCAAGAAGAGAGGGAAGAGAGGGCACTTAGGAAAGCAGAGATGGAGGCCAATAAGGCACAAAATATGGTTGAACATAAGGATGAAATTTATTCACGCCCTAAAAGAACCTGGTTTGAATCGGAAAGGGAAAAAAAAACTTGCAAAAGCATCTAAGGAATCAAAAGAGAGTACACAAACCACATCTGTTAAAAAGGTTACCAGTGTGCAAGAAGCAGATGATTTAAAAAGGAAAGAGAAAAGACGACTTGAGCATGAGAAAAATCTGCCACGGAAAAAGCGGAGGAAATTAGAAGCTGCTCGACAGATGCTAGAAGATGAGGAGGCAAATACCAATGATCATATGAAGGGATCCAAGAATGGTAATGGGGAGAAAGTTGGAAAGTCTGTAGTTGAGCTTGCATATCGCCGAGCGAAAGCTAGCAAGGCTGCTGAGAAGTCACGCGAAGCTGGTAAGTTCTTCAAGAAAGCTCCTTCAAAGAAACTTCAGAGGGATAAGGGACGTAAAGAAGAAATGGAGGATCTGTTCCAAGGTGATATGAGTGCCAAAAAGAAAGGGAGTACTAACACAGGTGGCTCAATGGGTGGTGGAGGGAAAAAGAAGTCAAAGAATGCATTCAAAAGCAAGTCAAGGTACAAGAGGCGGAAATAGACAACGGAAGTTTATCGATCGGGGATCCTCTAGAGTTGAGTAGATATAAGATTCTTCTATAGAAATAGCAAATTTTGTTGCTCCAAAGCTTTTGTAACTATTTGGGTAGAGTGTATTTTGATTTGTTACTCCAATAATCTGACAAGTTCAAAAGCCTTGTGTGACACTAACACGTGGGGGTCGAAGGTTGTGTAACCTGAGGATATAGTAAAAGCTTCTAATAGCAACTGCCATTGTATTCTATACACGTCAAAGGCAAAGTTATTATCTTGTCATTTTATGGTCAAAAACATAATTTTAATTTAACGTAATTGTTCAATGCATTATACATGCATCAATACCGTTGCAATATATCTGAGCTTAGCTTTGACC

Ace file of the UniGene sp_v3.0_unigene5465

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Dead box ATP-dependent RNA helicase, putative n=1 Tax=Ricinus communis RepID=B9R8B1_RICCO	-	-	0.0	79%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	38%
Sma3	DEAD-box ATP-dependent RNA helicase 28	-	-	2.786e-08	-

Source	ECs	Term	Type	e value	Identity
Sma3	Hydrolases, Acting on acid anhydrides, In phosphorous-containing anhydrides.	EC:3.6.1.-	-	4.329e-06	-

Source	KEGGs	Term	e value	Identity
Sma3	Purine metabolism	00230	4.329e-06	%
Sma3	Lipopolysaccharide biosynthesis	00540	4.329e-06	%
Sma3	Folate biosynthesis	00790	4.329e-06	%
Sma3	Metabolic pathways	01100	4.329e-06	%

Source	Gene names
Sma3	AT4G16630; At1g16280; At4g16630; CHLREDRAFT_129561; CHLREDRAFT_223; CHLREDRAFT_96655; F3O9.8; FCAALL.424; GSVIVT00013119001; GSVIVT00014396001; GSVIVT00029727001; GSVIVT00035915001; LOC_Os12g29660; MICPUCDRAFT_19576; MICPUCDRAFT_55574; MICPUN_83076; OSTLU

Source	GOs	Term	Type	Identity
Sma3	nucleic acid binding	GO:0003676	Molecular Function	-
Sma3	RNA binding	GO:0003723	Molecular Function	-
Sma3	helicase activity	GO:0004386	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	ATP-dependent helicase activity	GO:0008026	Molecular Function	-

Source	InterPros	Term	Type	Identity
Sma3	RNA helicase, ATP-dependent, DEAD-box, conserved site	IPR000629	-	-
Sma3	Helicase, C-terminal	IPR001650	-	-
Sma3	DNA/RNA helicase, DEAD/DEAH box type, N-terminal	IPR011545	-	-
Sma3	Helicase, superfamily 1/2, ATP-binding domain	IPR014001	-	-
Sma3	RNA helicase, DEAD-box type, Q motif	IPR014014	-	-
Sma3		IPR014021	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT4G16630.1	DEA(D/H)-box RNA helicase family protein chr4:9362176-9366449 REVERSE LENGTH=789	77%
RefSeq	Arabidopsis thaliana	NP_193396.3	DEAD-box ATP-dependent RNA helicase 28 [Arabidopsis thaliana]	77%
RefSeq	Populus trichocarpa	XP_002306942.1	predicted protein [Populus trichocarpa]	82%

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LKW8

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 207 aas, putative chimeric sequence, your sequence is shorter than subject: 521 - 802

Fln protein: CVKDDDVRLKETGRWDGQSDDDSESDDNDKPSPAKAPKKTRKENNGTNDTEMASDKKVSTGFFASAEGASFSANSFVELNLSRPLLRACESLGYKKPTPIQAACIPLALTGRDICGSAITGSGKTAAFALPVLERLLYRPRRIPAIRVLILTPTRELAVQVHSMTDKIAQFTDIRSCLVVGGLSTKAQESALRSRPDIVVATPGRMIDHLHNAQSVGLEELAVLILDEADRLLELGFSSEVQELVKLCPKRRQTMLFSATMTEEVDELVKLSLTSPVRLSADPSTKRPSTLTEEVIKIRPVHEAKQEAILLALCSRTFTSKAIIFSGTKQAAHRLKILFGLSDLKAAELHGNLTQAQRLDALEQFRKQEVDFLIATDVAARGLDIIGVKTVINFACPRDITSYVHRVGRTARAGKEGCAITFVTERDRRLLKAMAKRAGSKLLSRVVAEQSIAKWCQNIEQMEDDVASIIQEEREERALRKAEMEANKAQNMVEHKDEIYSRPKRTWFESEREKKTCKSI*

Protein Length: 522

Fln nts: AATGTGTAAAAGATGATGACGTCCGTCTGAAGGAGACAGGCCGCTGGGATGGCCAAAGCGACGATGATTCGGAGAGCGATGATAATGATAAACCCTCTCCTGCAAAAGCTCCCAAAAAAACCCGCAAGGAGAATAATGGGACGAATGACACAGAAATGGCAAGTGATAAGAAAGTCTCTACTGGATTTTTTGCATCAGCAGAAGGGGCTTCTTTTAGTGCAAATTCCTTTGTTGAATTAAATCTTTCACGCCCATTGCTGAGGGCATGTGAATCCCTTGGTTATAAAAAGCCAACTCCGATTCAGGCAGCATGTATACCTTTGGCATTAACTGGACGCGATATATGTGGAAGTGCTATTACTGGATCTGGAAAGACAGCTGCATTTGCTCTACCAGTGTTGGAGAGATTGCTTTACCGTCCTAGACGCATCCCGGCCATAAGAGTCCTTATTCTCACTCCAACGAGGGAACTTGCTGTTCAGGTACACAGTATGACAGACAAGATTGCACAGTTTACAGATATCCGCAGTTGCCTTGTTGTTGGGGGTCTGTCAACTAAGGCACAGGAATCAGCATTAAGGTCCCGGCCTGATATTGTTGTAGCAACGCCAGGGAGGATGATAGATCATTTGCACAATGCACAGTCAGTTGGACTGGAAGAACTTGCAGTATTGATTCTTGACGAGGCTGACCGTCTTCTGGAGCTTGGTTTTAGCAGTGAAGTTCAAGAGCTGGTTAAACTTTGTCCAAAACGAAGACAAACGATGTTGTTCTCAGCTACAATGACAGAGGAAGTGGATGAACTTGTCAAGCTTTCACTTACTAGTCCTGTGCGTCTTTCAGCAGATCCGTCGACAAAGAGGCCATCAACACTGACTGAAGAGGTAATAAAGATTCGACCTGTACATGAAGCCAAGCAGGAAGCAATATTGCTTGCATTATGTTCCCGAACCTTTACAAGCAAAGCAATTATCTTCAGTGGCACCAAACAAGCAGCACATCGCTTGAAAATCTTATTCGGACTCTCAGATTTGAAAGCTGCTGAGCTGCATGGAAATCTCACTCAAGCACAACGGCTTGATGCCCTTGAACAATTCAGAAAACAAGAAGTTGATTTTCTTATTGCGACTGATGTAGCAGCTCGGGGCCTGGACATCATCGGTGTTAAAACAGTAATAAACTTTGCATGTCCTCGTGATATCACATCCTATGTTCATAGAGTTGGTAGAACGGCAAGAGCTGGTAAGGAAGGCTGTGCCATCACTTTTGTTACAGAACGGGATCGCCGTCTTTTGAAAGCTATGGCAAAAAGAGCTGGCTCTAAGCTCCTGAGCAGGGTAGTTGCAGAACAGTCCATTGCTAAGTGGTGTCAGAATATTGAACAAATGGAGGATGATGTTGCTTCAATCATTCAAGAAGAGAGGGAAGAGAGGGCACTTAGGAAAGCAGAGATGGAGGCCAATAAGGCACAAAATATGGTTGAACATAAGGATGAAATTTATTCACGCCCTAAAAGAACCTGGTTTGAATCGGAAAGGGAAAAAAAAACTTGCAAAAGCATCTAA___GGAATCAAAAGAGAGTACACAAACCACATCTGTTAAAAAGGTTACCAGTGTGCAAGAAGCAGATGATTTAAAAAGGAAAGAGAAAAGACGACTTGAGCATGAGAAAAATCTGCCACGGAAAAAGCGGAGGAAATTAGAAGCTGCTCGACAGATGCTAGAAGATGAGGAGGCAAATACCAATGATCATATGAAGGGATCCAAGAATGGTAATGGGGAGAAAGTTGGAAAGTCTGTAGTTGAGCTTGCATATCGCCGAGCGAAAGCTAGCAAGGCTGCTGAGAAGTCACGCGAAGCTGGTAAGTTCTTCAAGAAAGCTCCTTCAAAGAAACTTCAGAGGGATAAGGGACGTAAAGAAGAAATGGAGGATCTGTTCCAAGGTGATATGAGTGCCAAAAAGAAAGGGAGTACTAACACAGGTGGCTCAATGGGTGGTGGAGGGAAAAAGAAGTCAAAGAATGCATTCAAAAGCAAGTCAAGGTACAAGAGGCGGAAATAGACAACGGAAGTTTATCGATCGGGGATCCTCTAGAGTTGAGTAGATATAAGATTCTTCTATAGAAATAGCAAATTTTGTTGCTCCAAAGCTTTTGTAACTATTTGGGTAGAGTGTATTTTGATTTGTTACTCCAATAATCTGACAAGTTCAAAAGCCTTGTGTGACACTAACACGTGGGGGTCGAAGGTTGTGTAACCTGAGGATATAGTAAAAGCTTCTAATAGCAACTGCCATTGTATTCTATACACGTCAAAGGCAAAGTTATTATCTTGTCATTTTATGGTCAAAAACATAATTTTAATTTAACGTAATTGTTCAATGCATTATACATGCATCAATACCGTTGCAATATATCTGAGCTTAGCTTTGACC

Fln Alignment:

Contig5465___LNLSRPLLRACESLGYKKPTPIQAACIPLALTGRDICGSAITGSGKTAAFALPVLERL---------LYRPRRIPAIRVLILTPTRELAVQVHSMTDKIAQFTDIRSCLVVGGLSTKAQESALRSRPDIVVATPGRMIDHLHNAQSVGLEELAVLILDEADRLLELGFSSEVQELVKLCPKRRQTMLFSATMTEEVDELVKLSLTSPVRLSADPSTKRPSTLTEEVIKIRPVHEAKQEAILLALCSRTFTS--KAIIFSGTKQAAHRLKILFGLSDLKAAE-LHGNLTQAQRLDALEQFRKQEVDFLIATDVAARGLDIIGVKTVINFACPRDITSYVHRVGRTARAGKEGCAITFVTERDRRLLKAMAKRAGSK
B8LKW8______________LNLSQELVEALANRGITHLFPIQRAVLVPALEGRDLIGRAKTGTGKTLAFGIPIIERLTKDNKENKLLRQSRRLP--RVLVLAPTRELAKQVEKEFKESAPF--LSTACIYGGVSYVSQQNALARGVDVVVGTPGRIID-LVNSKSLKLNEVQFLVLDEADQMLAVGFEEDVEIILDNLPAERQSMLFSATMPGWVKKLSRKHLNNPMTIDLVGENEEKLAEGIKLYAVATTTSSNSKRSILGDLITVFAKGGKTIVFTQTKRDADEISL--GLSSTIACEALHGDISQHQRERTLAGFREGKFSALVATDVAARGLDITNVDLVIHYEFPNDPETFVHRSGRTGRAGKEGIAILMYPSNGKRKVRSLEHEVGCR

SNPs (tot: 23)

Position	A %	C %	G %	T %	Probability
451	90		9		0.999842
726			11	88	0.923564
745	12			87	0.992554
746		12	87		0.992554
748		87		12	0.992554
933	11		88		0.923564
998	14	85			0.993533
1020		66		33	1.0
1024	11	88			0.923564
1026		88	11		0.923564
1027			11	88	0.923564
1028		88	11		0.923564
1142		80		20	0.995493
1903	75		25		0.996473
2169	20		80		0.995493
2171	80		20		0.995493
2173	80		20		0.995493
2225			83	16	0.994512
2226	33		66		1.0
2241	16		83		0.994512
2328			7	92	0.999532
2405		66		33	1.0
2433	85			14	0.993533

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain