SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene3788

Length: 1809 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v3.0_unigene3788 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Trans-cinnamate 4-hydroxylase n=2 Tax=Pinaceae RepID=Q9XEH8_PINTA	-	-	71%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	73%
Sma3	Cinnamic acid 4-hydroxylase	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3	Trans-cinnamate 4-monooxygenase.	EC:1.14.13.11	-	0.0	-

Source	KEGGs	Term	Identity
Sma3	Phenylalanine metabolism	00360	%
Sma3	Phenylpropanoid biosynthesis	00940	%
Sma3	Flavonoid biosynthesis	00941	%
Sma3	Stilbenoid, diarylheptanoid and gingerol biosynthesis	00945	%
Sma3	Biosynthesis of phenylpropanoids	01061	%
Sma3	Biosynthesis of alkaloids derived from shikimate pathway	01063	%
Sma3	Metabolic pathways	01100	%
Sma3	Biosynthesis of secondary metabolites	01110	%

Source	Gene names
Sma3	BcCyp450; C4H; C4H-1; C4H-2; C4H1; C4H1-1; C4H1-2; C4H2; C4H2-1; C4H2-2; C4H2\|CYP73A42; C4H3; CYP73; CYP73A1; CYP73A10; CYP73A11; CYP73A12; CYP73A13; CYP73A14; CYP73A16; CYP73A19; CYP73A2; CYP73A27; CYP73A28; CYP73A3; CYP73A4; CYP73A43\|C4H1; CYP73A5; CYP7

Source	GOs	Term	Type	Identity
Sma3	endoplasmic reticulum	GO:0005783	Cellular Component	-
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	plant-type cell wall	GO:0009505	Cellular Component	-
Sma3	monooxygenase activity	GO:0004497	Molecular Function	-
Sma3	iron ion binding	GO:0005506	Molecular Function	-
Sma3	electron carrier activity	GO:0009055	Molecular Function	-
Sma3	trans-cinnamate 4-monooxygenase activity	GO:0016710	Molecular Function	-
Sma3	heme binding	GO:0020037	Molecular Function	-
Sma3	oxidation-reduction process	GO:0055114	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Cytochrome P450	IPR001128	-	-
Sma3	Cytochrome P450, E-class, group I	IPR002401	-	-
Sma3	Aldehyde dehydrogenase, conserved site	IPR016160	-	-
Sma3	Cytochrome P450, conserved site	IPR017972	-	-
Sma3		IPR017973	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT2G30490.1	ATC4H, C4H, CYP73A5, REF3 cinnamate-4-hydroxylase chr2:12993861-12995683 REVERSE LENGTH=505	63%
RefSeq	Arabidopsis thaliana	NP_180607.1	trans-cinnamate 4-monooxygenase [Arabidopsis thaliana]	63%
RefSeq	Populus trichocarpa	XP_002325637.1	cytochrome P450 [Populus trichocarpa]	66%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: C0PRB9

Fln msg:

Fln protein: MSEQLVGAAMEKLDLQKGLLALFALIVAAILGSKLKSKKLKLPPGPPAVPLFGNWLQVGDDLNQRNLTDLAKKYGEIFLLKMGQRNLLVVASPELAKEVLQTQGVEFGSRPRNVVYDIFTGSGQDMIFTYYGHHWRKMRRIMTAPFFTHNVVQKYKFAWEEEIGRAMEDIRARPEASSEGIVIRGRMQLMVYNIMYKMLFDRRFESEEDPLFLRLKTLNAQRSRMGQSFEYNYGDFIPILSPLLRGYMKICNQVKEKRQALFTESFIKERKKLGITQGDRKCGMDYIFEAQEKGEINEENILYLIDNMNTAGMETIVWTMEWVIAELVNHPEIQQKIRDELDAVVGPGIPMAESDIVKLPYLQAVMKESLRLHITLPFVPRLNVPQTKLGGYDIPAGTKVLVNIWWLANNPELWDNPQDFIPERFLQEGEKIEFHGNDFRFLPFGAGRRSCPGIHMALANVALTVGRLLQNFELLPPPGKSKVDVSDKGGQFSLPILNHTLLVAKPRLPASS*

Protein Length: 514

Fln nts: CGTCGTGACCTTTGTTGTGACTTGTGTGGCAATTCAGAGTCTGAGC_-_ATGTCAGAGCAACTCGTTGGTGCAGCAATGGAGAAGTTAGATCTACAGAAAGGCCTTTTGGCTCTCTTCGCTCTAATAGTGGCTGCTATTTTGGGGTCGAAGCTGAAATCTAAGAAGCTGAAACTTCCTCCGGGTCCGCCTGCGGTGCCCCTATTTGGGAACTGGCTACAGGTGGGAGACGATCTGAATCAAAGAAATCTGACCGACTTAGCAAAGAAGTATGGCGAGATCTTTCTGCTGAAGATGGGGCAGAGGAATCTCCTGGTGGTGGCGTCGCCGGAGCTGGCAAAGGAGGTGCTGCAGACGCAGGGGGTGGAGTTCGGGTCCCGCCCGAGGAACGTGGTGTACGATATATTTACAGGGAGTGGGCAGGACATGATCTTCACTTATTACGGACACCATTGGCGGAAGATGCGGAGGATCATGACGGCGCCCTTCTTTACCCACAATGTTGTCCAGAAATACAAATTTGCGTGGGAAGAGGAGATCGGCCGCGCGATGGAGGATATCAGGGCTCGGCCCGAGGCGTCGAGTGAGGGGATTGTGATCAGGGGACGCATGCAGCTAATGGTGTATAATATTATGTACAAGATGTTGTTTGACAGGAGGTTTGAGAGCGAGGAGGATCCCCTCTTTCTTCGCCTCAAAACTCTCAACGCCCAGAGGAGCCGCATGGGCCAGAGCTTCGAATATAACTATGGCGATTTTATTCCCATCCTCAGTCCCTTGCTCAGAGGATATATGAAAATCTGCAACCAGGTCAAGGAAAAGAGGCAGGCGCTGTTCACGGAATCTTTTATCAAAGAGCGAAAGAAATTGGGAATTACCCAGGGCGACCGGAAGTGTGGGATGGACTACATATTTGAGGCGCAGGAGAAAGGCGAAATAAACGAAGAGAACATTCTATATCTGATTGACAACATGAATACTGCTGGAATGGAGACGATTGTGTGGACAATGGAATGGGTTATTGCAGAACTGGTGAACCATCCCGAGATTCAGCAGAAGATCCGAGACGAACTGGATGCCGTCGTGGGGCCGGGCATCCCAATGGCTGAGTCTGACATTGTGAAACTGCCATACCTGCAAGCCGTCATGAAGGAATCTCTGCGTTTACACATTACTCTTCCCTTCGTCCCTCGGTTGAACGTTCCCCAGACGAAGCTCGGCGGGTATGACATCCCGGCGGGGACCAAAGTGCTCGTCAATATCTGGTGGCTGGCCAACAATCCCGAGCTGTGGGACAACCCCCAAGATTTCATTCCTGAGAGGTTTCTGCAGGAGGGTGAGAAGATTGAATTCCACGGAAATGATTTCAGGTTCCTCCCATTCGGCGCAGGGAGGAGAAGTTGCCCTGGAATCCACATGGCTCTTGCAAATGTGGCGCTGACCGTTGGAAGGCTGCTTCAGAACTTCGAGCTTCTGCCTCCGCCGGGGAAATCGAAGGTGGATGTAAGTGACAAAGGGGGTCAATTCAGCTTGCCAATTCTCAACCACACCCTGCTTGTAGCCAAACCCAGACTCCCAGCCTCAAGCTGA___ACGTCCGACAGCTGCACCCAATAAAAGCCTACGTTGCTTTTTTCGTTGTGTTGATTTCTGTAACAGCAACGTAGTTGTTGAATTTTAGGTTTTGATTGGTTGTACTCTAGCTTCTCTCGTCGGCAGGGAGGGAATCATGGTGTCAAACAACATGACTCTCAATCTTGCTGGGCTTATTTCAAATAAAAAATGTAAGTGGGTATATTTTTCTAGTGCCAAACAGA

Fln Alignment:

Contig3788___AVPLFGNWLQVGDDLNQRNLTDLAKKYGEIFLLKMGQRNLLVVASPELAKEVLQTQGVEFGSRPRNVVYDIFTGSGQDMIFTYYGHHWRKMRRIMTAPFFTHNVVQKYKFAWEEEIGRAMEDIRARPEASSEGIVIRGRMQLMVYNIMYKMLFDRRFESEEDPLFLRLKTLNAQRSRMGQSFEYNYGDFIPILSPLLRGYMKICNQVKEKRQALFTESFIKERKKLGITQGDR----KCGMDYIFEAQEKGEINEENILYLIDNMNTAGMETIVWTMEWVIAELVNHPEIQQKIRDELDAVVGPGIPMAESDIVKLPYLQAVMKESLRLHITLP-FVPRLNVPQTKLGGYDIPAGTKVLVNIWWLANNPELWDNPQDFIPERFLQEGEKIEFHGNDFRFLPFGAGRRSCPGIHMALANVALTVGRLLQNFELLPPPGKSKVDVSDKGGQFSLPILNHTLLVAKPR
C0PRB9______________AVPIFGNWLQVGDDLNHRNLGDLAKKYGEIFLLKMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQYRFAWEDEIGRAVDDIKKRPEASTTGIVIRRRLQLVMYNIMYRMMFDRRFESEEDPLFLRLKALNGERSRLAQSFEYNYGDFIPVLRPFLRGYLKICKDVKERRLTLFKNYFVDERKKLGSTMGSNVTGDKCAIDHIFEAQDKGEINEDNVLYIVENINVAAIETTLWSMEWGIAEIVNHPEIQQKIRDELDAVVGRGVPLTEPDTVRLPYLQAVVKETLRLHMAIPLLVPHMNLHQAKLGGYDIPAESKILVNAWFLANNPEWWKNPEEFIPERFL-EDEKIEASGNDFRFLPFGVGRRSCPGIILALPILSLAIGRLVQNFELLPPPGQSNVDVSEKGGQFSLHILNHSVVVAKPR

SNPs (tot: 39)

Position	A %	C %	G %	T %	Probability
639	25	75			0.996473
657	25		75		0.996473
710		25		75	0.996473
816	25		75		0.996473
1115		33		66	0.997452
1174		60		40	1.0
1176	40		60		1.0
1252		80		20	0.995493
1299		16	83		0.994512
1300		16		83	0.994512
1301	83	16			0.994512
1302		16		83	0.994512
1303	16			83	0.994512
1311	80		20		0.995493
1334		60		40	1.0
1362	40		60		1.0
1413	60		40		1.0
1431		50		50	1.0
1460			20	80	0.995493
1463	20		80		0.995493
1537		60	40		1.0
1614			50	50	1.0
1671		28		57	1.0
1707	50		50		1.0
1777	50		50		1.0
1779	16		16	66	0.988813
1782	20	80			0.995493
1786		60	40		1.0
1788	40	60			1.0
1789		75		25	0.996473
1790	50		25	25	0.995533
1792			75	25	0.996473
1793		25	25		0.995533
1794		25		75	0.996473
1795		75	25		0.996473
1797	25	50	25		0.995533
1798	50		25	25	0.995533
1799	75		25		0.996473
1801	33	33	33		0.997753

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain