SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene3273

Length: 2627 nt

UniGene Fasta

>sp_v3.0_unigene3273
AGACTGGAAGAACTCTCTGAGGCAATGCTTCTGGCAGACCTCGATTTAGATACGGTTGATTTTGTCCCAAATTTTGATGGGTCACAAAAGGAACCATCTTTATTACCTGCCAGGCTCCCAAATGTACTGCTAAATGGCGCTACAGGAATAGCTGTTGGTATGGCAACCTATATACCTCCTCACAATCTTGGAGAGGTTGCAGATGCATTGCTTGCTTTTATTCATAATCCTGATGCCACGATTCATGAACTGATGGAATACATGCCTGGACCGGACTTCCCTACTGGTGGATTAATTATGGGTAATTCTGGGATCTCAGCAGCATATAGAACAGGTCGAGGGAGTGTGACACTAAGAGGAAGGACTGAGATTGAGGTGATTGACAAGAAAACCATGCGCAGTGCCATTATTATTAGAGAGATCCCTTATCAGACGAACAAGGCTGCATTAGTGGAGAAAATTGCTGATCTGGTAAATGACAAGGCTTTGGATAGTGTTAGCGACATACGAGATGAAAGTGACCGTACAGGGATGCGAATAGTACTGGAGTTGAAAAAAGGTTCAGAGGCAGCTATTGTTCTGAATTATCTCTACAGGCACACTTCCCTTCAATCTAATGTTAATTACAACATGGTGGGAATTCTTGATGGACGGCCTAAATTAATGGGTCTAAGGGACTTGCTTCAGGCTTTCCTAGATTTTAGATGCTCAGTTGTTGAACGACGTGCAAAATATCAGTTGAGGGTTGCCGAAGAAAGGGAACATATTGTGAAGGGTAATGTAATATGCTTGGACAATGTTGATGGAGTAATACATATTATTAGGAAATCTTCAAGCCATTCTGAAGCAATTGTTGCTATGATGGAAGCATATAATTTATCAGAAAAGCAAGCTCAGGCAATCTTGGATATCACTCTTCGAAGACTTACATTGTTTGAGAGAAGCAAGTTTGTTGAGGAGGACAGATCATTGACTGGACAAATTTCAAGTTTAAAGCAACTATTAGCAAACAAGCAGGAAATTTTGCAGGTTATAGAAAAAGAGGCACTTGAACTAAAGAAAAGGTTTGGAACACCAAGAAGATCATCAATAGAGGAAGCGCCAAATGGACAGCTAGATGAAATAGATGTAATTCCAAATGAGGAGATGCTTTTGGCCCTTAGTGAGAAGGGATATATGAAACGGATGAAGCCCGATATGTTTAGCCTACAACGACGTGGAACAATTGGAAAATCACTTGGGAAGCTGAGGGGAAATGATGCCATGTCAGAATTTTCCTTTTGCCGTAACCATGATCATATACTATATTTCAGTGACAAGGGCATAGTATATTCTGCACGTGCTTATCGAGTACCAGAGTGCTCACGAAGTGCTGCAGGGACGCCACTTGTTAAGATTCTTTCATTATCCGCAGGAGAAAGAATAACTTCTATTATACCAGTTAGCGAATTCAGTGCAGATCAGTATTTGATTATGTTGACTGCCAATGGCTATATCAAGAGAGCCTCTTTGGATTTTTTCTCATCTATCAGATCCGCGGGGATTATTGGTATTCAACTTGTGCCTGAGGATGAGCTAAAGTGGGTAAGGCGGTGTACAGATGAAGACATTATTGCAATTGTTTCCCAAAATGGGATGCTCACGTTGAGCTCATGTGGGATGCTCCGAGCTAGCGGTAGGAAAACTAGAGGATGGACATCGATGAAATTGAAGGGTGAAGATAAGGTTGCATCTATGGATATTGTTCCAGCTGCAGTGCAGCAGGAGCTAAAGAACACTTTACATAGCTCAAAGTATCAGGGAAAAACTATAAGTGCTCCATGGCTTCTTCTTGTGTCTGAGAGTGGATATGGAAAGCGTGTACCCCTAAGTAGTTTTCGAATTACACCTTTAAATCGAATTGGTGTAATTGGCTACAAGCTCTTGCCAGAGGATCGCGTTGCTGCATTATTTGTTGTTGGACTATCGCTTTCAGATGATGGTGAAAGTGATGAACAAGTTGTTCTTGTGAGCCACAGTGGCATGGTGAACAGAATTATGGTTCGAGACATTTCTATCCAGTCGAGAACTGCCAAGGGTGTGAGACTAATGAGACTAGATTATGGTGGCAAGATTCAGTCAGTTTCTGTAGTAGGTCCTTCAGAAATTCACAGCGATGGACCTGAACTGGAAGCAGTTAATCCCTAGTTGCTCTTATTTTTCAAGTTTACGTTGTAATATTAGGGCATCAAAATGAAGAACACTCTTAATTTTGGATCATTTTATTATTTTCTAGGTGGCACTGTATACATATTGTATTAAGATTACATTAAGTGTTCTGTGAGAAGTAGCAGCGAGAAGATATACAAAGTCTGTGAGCCTTATTTGTCACAGCAGCAATTTTGAATATCCGGAAATATACGTCATGTGAAATGTATTCTGAGAGTACATCATTATTATATTGGAATTGAATCTGACGGTGGAAGATAATTATCTTTCCTCAATTACCTAAGATAAGCCCAATTAGCATATCAACGAGATAACATGATAATTAGCAAATAGCATGTCAGGTGAATAAAAGCAATTTTGACATAATATTTAATGTTCATATTGTCCATTCATGCACTGCACGTTCAGTCCACTTATCA

Ace file of the UniGene sp_v3.0_unigene3273

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	DNA gyrase subunit A, chloroplastic/mitochondrial n=1 Tax=Nicotiana benthamiana RepID=GYRA_NICBE	-	-	0.0	67%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	88%
Sma3	Probable DNA gyrase subunit A, chloroplastic/mitochondrial	-	-	5.669e-09	-

Source	ECs	Term	Type	e value	Identity
Sma3	DNA topoisomerase (ATP-hydrolyzing).	EC:5.99.1.3	-	6.617e-12	-

Source	Gene names
Sma3	At3g10690; CHLREDRAFT_115934; F13M14.2; GSVIVT00033900001; GSVIVT00033901001; GYRA; LOC_Os03g59750; MtrDRAFT_AC150269g2v2; OSJNBa0024F18.37; OSJNBb0033J23.17; Os03g0812000; OsI_14033; OsJ_13077; Ot17g01710; PHYPADRAFT_135715; POPTRDRAFT_805680; RCOM_07202

Source	GOs	Term	Type	Identity
Sma3	chromosome	GO:0005694	Cellular Component	-
Sma3	mitochondrion	GO:0005739	Cellular Component	-
Sma3	nucleoid	GO:0009295	Cellular Component	-
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	DNA topoisomerase activity	GO:0003916	Molecular Function	-
Sma3	DNA topoisomerase (ATP-hydrolyzing) activity	GO:0003918	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	DNA topological change	GO:0006265	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	DNA topoisomerase, type IIA, subunit A/C-terminal	IPR002205	-	-
Sma3	DNA gyrase, subunit A	IPR005743	-	-
Sma3	DNA gyrase/topoisomerase IV, subunit A, C-terminal beta-pinwheel	IPR006691	-	-
Sma3	DNA topoisomerase, type IIA, subunit A, alpha-helical	IPR013757	-	-
Sma3	DNA topoisomerase, type IIA, subunit A/ C-terminal, alpha-beta	IPR013758	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT3G10690.1	GYRA DNA GYRASE A chr3:3339612-3346243 REVERSE LENGTH=950	64%
RefSeq	Arabidopsis thaliana	NP_187680.2	DNA gyrase subunit A [Arabidopsis thaliana]	64%
RefSeq	Populus trichocarpa	XP_002315402.1	predicted protein [Populus trichocarpa]	65%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5ABZ8

Fln msg: your sequence is longer than subject: 721 - 183

Fln protein: MLLADLDLDTVDFVPNFDGSQKEPSLLPARLPNVLLNGATGIAVGMATYIPPHNLGEVADALLAFIHNPDATIHELMEYMPGPDFPTGGLIMGNSGISAAYRTGRGSVTLRGRTEIEVIDKKTMRSAIIIREIPYQTNKAALVEKIADLVNDKALDSVSDIRDESDRTGMRIVLELKKGSEAAIVLNYLYRHTSLQSNVNYNMVGILDGRPKLMGLRDLLQAFLDFRCSVVERRAKYQLRVAEEREHIVKGNVICLDNVDGVIHIIRKSSSHSEAIVAMMEAYNLSEKQAQAILDITLRRLTLFERSKFVEEDRSLTGQISSLKQLLANKQEILQVIEKEALELKKRFGTPRRSSIEEAPNGQLDEIDVIPNEEMLLALSEKGYMKRMKPDMFSLQRRGTIGKSLGKLRGNDAMSEFSFCRNHDHILYFSDKGIVYSARAYRVPECSRSAAGTPLVKILSLSAGERITSIIPVSEFSADQYLIMLTANGYIKRASLDFFSSIRSAGIIGIQLVPEDELKWVRRCTDEDIIAIVSQNGMLTLSSCGMLRASGRKTRGWTSMKLKGEDKVASMDIVPAAVQQELKNTLHSSKYQGKTISAPWLLLVSESGYGKRVPLSSFRITPLNRIGVIGYKLLPEDRVAALFVVGLSLSDDGESDEQVVLVSHSGMVNRIMVRDISIQSRTAKGVRLMRLDYGGKIQSVSVVGPSEIHSDGPELEAVNP*

Protein Length: 722

Fln nts: AGACTGGAAGAACTCTCTGAGGCA_-_ATGCTTCTGGCAGACCTCGATTTAGATACGGTTGATTTTGTCCCAAATTTTGATGGGTCACAAAAGGAACCATCTTTATTACCTGCCAGGCTCCCAAATGTACTGCTAAATGGCGCTACAGGAATAGCTGTTGGTATGGCAACCTATATACCTCCTCACAATCTTGGAGAGGTTGCAGATGCATTGCTTGCTTTTATTCATAATCCTGATGCCACGATTCATGAACTGATGGAATACATGCCTGGACCGGACTTCCCTACTGGTGGATTAATTATGGGTAATTCTGGGATCTCAGCAGCATATAGAACAGGTCGAGGGAGTGTGACACTAAGAGGAAGGACTGAGATTGAGGTGATTGACAAGAAAACCATGCGCAGTGCCATTATTATTAGAGAGATCCCTTATCAGACGAACAAGGCTGCATTAGTGGAGAAAATTGCTGATCTGGTAAATGACAAGGCTTTGGATAGTGTTAGCGACATACGAGATGAAAGTGACCGTACAGGGATGCGAATAGTACTGGAGTTGAAAAAAGGTTCAGAGGCAGCTATTGTTCTGAATTATCTCTACAGGCACACTTCCCTTCAATCTAATGTTAATTACAACATGGTGGGAATTCTTGATGGACGGCCTAAATTAATGGGTCTAAGGGACTTGCTTCAGGCTTTCCTAGATTTTAGATGCTCAGTTGTTGAACGACGTGCAAAATATCAGTTGAGGGTTGCCGAAGAAAGGGAACATATTGTGAAGGGTAATGTAATATGCTTGGACAATGTTGATGGAGTAATACATATTATTAGGAAATCTTCAAGCCATTCTGAAGCAATTGTTGCTATGATGGAAGCATATAATTTATCAGAAAAGCAAGCTCAGGCAATCTTGGATATCACTCTTCGAAGACTTACATTGTTTGAGAGAAGCAAGTTTGTTGAGGAGGACAGATCATTGACTGGACAAATTTCAAGTTTAAAGCAACTATTAGCAAACAAGCAGGAAATTTTGCAGGTTATAGAAAAAGAGGCACTTGAACTAAAGAAAAGGTTTGGAACACCAAGAAGATCATCAATAGAGGAAGCGCCAAATGGACAGCTAGATGAAATAGATGTAATTCCAAATGAGGAGATGCTTTTGGCCCTTAGTGAGAAGGGATATATGAAACGGATGAAGCCCGATATGTTTAGCCTACAACGACGTGGAACAATTGGAAAATCACTTGGGAAGCTGAGGGGAAATGATGCCATGTCAGAATTTTCCTTTTGCCGTAACCATGATCATATACTATATTTCAGTGACAAGGGCATAGTATATTCTGCACGTGCTTATCGAGTACCAGAGTGCTCACGAAGTGCTGCAGGGACGCCACTTGTTAAGATTCTTTCATTATCCGCAGGAGAAAGAATAACTTCTATTATACCAGTTAGCGAATTCAGTGCAGATCAGTATTTGATTATGTTGACTGCCAATGGCTATATCAAGAGAGCCTCTTTGGATTTTTTCTCATCTATCAGATCCGCGGGGATTATTGGTATTCAACTTGTGCCTGAGGATGAGCTAAAGTGGGTAAGGCGGTGTACAGATGAAGACATTATTGCAATTGTTTCCCAAAATGGGATGCTCACGTTGAGCTCATGTGGGATGCTCCGAGCTAGCGGTAGGAAAACTAGAGGATGGACATCGATGAAATTGAAGGGTGAAGATAAGGTTGCATCTATGGATATTGTTCCAGCTGCAGTGCAGCAGGAGCTAAAGAACACTTTACATAGCTCAAAGTATCAGGGAAAAACTATAAGTGCTCCATGGCTTCTTCTTGTGTCTGAGAGTGGATATGGAAAGCGTGTACCCCTAAGTAGTTTTCGAATTACACCTTTAAATCGAATTGGTGTAATTGGCTACAAGCTCTTGCCAGAGGATCGCGTTGCTGCATTATTTGTTGTTGGACTATCGCTTTCAGATGATGGTGAAAGTGATGAACAAGTTGTTCTTGTGAGCCACAGTGGCATGGTGAACAGAATTATGGTTCGAGACATTTCTATCCAGTCGAGAACTGCCAAGGGTGTGAGACTAATGAGACTAGATTATGGTGGCAAGATTCAGTCAGTTTCTGTAGTAGGTCCTTCAGAAATTCACAGCGATGGACCTGAACTGGAAGCAGTTAATCCCTAG___TTGCTCTTATTTTTCAAGTTTACGTTGTAATATTAGGGCATCAAAATGAAGAACACTCTTAATTTTGGATCATTTTATTATTTTCTAGGTGGCACTGTATACATATTGTATTAAGATTACATTAAGTGTTCTGTGAGAAGTAGCAGCGAGAAGATATACAAAGTCTGTGAGCCTTATTTGTCACAGCAGCAATTTTGAATATCCGGAAATATACGTCATGTGAAATGTATTCTGAGAGTACATCATTATTATATTGGAATTGAATCTGACGGTGGAAGATAATTATCTTTCCTCAATTACCTAAGATAAGCCCAATTAGCATATCAACGAGATAACATGATAATTAGCAAATAGCATGTCAGGTGAATAAAAGCAATTTTGACATAATATTTAATGTTCATATTGTCCATTCATGCACTGCACGTTCAGTCCACTTATCA

Fln Alignment:

Contig3273___MLTLSSCGMLRASGRKTRGWTSMKLKGEDKVASMDIVPAAVQQELKNTLHSSKYQGKTISAPWLLLVSESGYGKRVPLSSFRITPLNRIGVIGYKLLPEDRVAALFVVGLSLSDDGESDEQVVLVSHSGMVNRIMVRDISIQSRTAKGVRLMRLDYGGKIQSVSVVGPSEIHSDGPELEAVNP
D5ABZ8______________MLTLSLCGMLRAIGRKSRGLISMRLRGEDKVASMDIVPAAVQQELKNALLSSKSQGKTITAPWLLLVSESGYGKRVPLSSFRITPLKRVGLIGYKLLPEDRVAALFVVGLSLSDDGESDEQVVLVSHSGMVNRIMVRAISIQSRTAKGVRLMRLEHGGKIQSVSVVGPSEVQSDGPELQAVVP

SNPs (tot: 19)

Position	A %	C %	G %	T %	Probability
2084		13	86		0.999973
2091		13	86		0.999973
2092	80	20			1.0
2093		85		14	0.999981
2095	85		14		0.999981
2096	85		14		0.999981
2099	85			14	0.999981
2100		14	85		0.999981
2104	85	14			0.999981
2105	14		85		0.999981
2171			84	15	0.999986
2172	84		15		0.999986
2173	76	7	15		0.999983
2177			84	15	0.999986
2178	76	23			1.0
2180		15	84		0.999986
2183		7	76		0.999983
2218	10		90		0.90298
2496			16	83	0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain