SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene714

Length: 4128 nt

UniGene Fasta

>sp_v3.0_unigene714
TCCTTCAACTGCTTTCCTCGTGGTTTGCAACGGCACTGGTTTGGATCTGGCTACCGCTTGAAGGATTATCACCTCTTTTCCACAAAATTTGCACTTTACGCAACCTTTTATTGTATTGAAGATCGGGTTCAGAATAAGGGTCATTCCTTACTTGCAGGGCACAGTACCTTCCGAGGAGATACTGCTACTTATTTTTTAGTACAGATTCCTCTTACTCTGGAGAACCTGGATGCAAATAAGTTTCAGACTATGCTTTTGAAACCTAAGCATAAAGGTGAAAAGCAAGCTAGAAGGCAGCTGTGATGAAACCGAAGGGCTGAACTTGCACAAGATGCTACGGGTGCTTTTGGGTGCATCTAATTCTGGAAGGATGTATGGGATATGTGTTTCCCAACATAATTCTACTTTGGAAAGAAGGCATATTCTTGGAGGAATGTGGTCTGTGTCATTATGTAAAAATGGATTTGTGATTAGTCAAGGCACTTTACCATTTTCAAGGTGCTGGGTGACTTTGTATGGGAGGACAAATAATCTCCAATATCAGCCTGCTAAATGTTGCTATGGTTTTATACAACCGAGCATGCCTAGCATGATTTGTCAATCAAACAATCTTACAGTGCTTTCTCACACCACTATAGTTTCAGCTTCGAATACTGAATCAAGTACTAAGGTTCTTCAGGATAAACATTTGTCAAGTGCAATCTTTTATGAGGCAAAAAATGGTTTTAGAGATGCTCAAGGGAAGAATATGAGGTTCAGTAACTTTCTATATTCAAAGCTTTTACGGTTATGTTCTCCTTTAGTTCATAAGTCTGCTCAAAGTTTATTAGAGGAAGTGAAGAACTACTCTCGATTGTGCATATCAGATCGTTTGCAGATTCTTAACAAGGTGTCCTTCCTTATGGGATATGGCAGTGTAGATGATTTGATTGGCCATGAAAGGGCTTTAAGGGAGTGTGCACGCCCGCTTCAAGAAGGGCCAAGTTCTTCACATTGCTTGCCAATTAGTTCTAGACATCATGCAGATGAAGTAAGTGGGTATCCATCTTCACTGGATTACTCTTATGTTTCAGAAGCTTGTCTGAAATTCTCGTCTATTCATTTGGGAAGCAGCCCACAACTGGATCTATATAGTGCAGCTGAGGAGAAGTCAGAATGGGTTGATTGTCAACTCATTAAGGACGTGGACAAATCTACTCAGCCAGTAGATGGCACTACTTTCGATTCACCAAATATCACAGAAACTGAATGTATAGTTAGGGACCAATCTAATCTTTTCACTGCTTGCCCGGTATCAAATGCTGAGCCAAATCTGTGGACACTCCAGTTAGATAAGCAAGACTTGCATTTATCAAAACAGAACAGGTCAGAAACTGTACAGAAGGAAGTTTTAGAAGGCAGTGCCACGGGCAATGAAAATATGTTACTGAGGAAGTCTACATGTAAAGTAATGGAAGCAACTGTTCCTGTCAATTGCTTCCTTGATGCACCTATTAACACTATAAAGGGAATACGACCAGCACAATGCCGTAAACTGGAAGAGAATGGATTTCACACGGTGCGTAAACTACTTCAACATTTCCCTCGTACTTATGTGAATATGCAAGCTGCTGAAGGCCAAGTAGAGGATGGGCAGTTGCTGAATTTCTTTGGAAGGATTTTATCTTCTAGAGGACGAAGGGCAGGGTATAGTGGTGGGATTGTTGAAGTCATTATTGGGTGTGAAATCCCTAGCAGTGGTGAATATCGTACTAGAAGTGATGAAGATTATCAGGATAAGGGAAAAAGGACTGTTTATATTCATTTGAAGAGATATTTCAGAGGAGTACGATTCACAAATACTTGGATTCTTGATAAATTGCAGTCGAAATATAATGAAGGTGATCTAGTATCAGTGAGTGGTAAGGTCAAGGCTTTAGAAGAAGCTGATCATTTTGAAATTCGGGAATATAACATTGATGTGCTGAAAGAGGAGGCAGATCTTACAAGGTCAGAAGTATGTGGAAAAGAGGAGCCTTGTCCTATTTATCCTTCAAAAGGTGATTTGGATCCAAGATACATAGGGGAATGCATTAGGAGAGTATTAGAAGTTTTACCTGCAGAGATGGATCCTCTTCCGGTCAATGTTTGTCAAAAGTTCAACTTGATGAAGCTTCGTGAAGCTTACTTTGGCATCCATTGTCCCAAGGATTTAAAGGAAGCTGACCATGCGCGCAGGAGGTTGATCTTTGATGACTTCTTTTATCATCAGTTGAGTTTATTGTATCAAAGGTATGGAGTGGTAGGCACATATATCAAAACTGGTGGTTTGCCCGAGAGTTCAACAGGGCTCGAAGTTGGAAGTATTGGTGTAGATAAATGGTCACAACTGACTTTGAAAGTGCTGAAATCACTTCCTTTTGCACTTACCAGTGCTCAGTTGAAAGCTGCATCAGAAATTATTTGGGACCTCAGAAGGCCAGTACCAATGAGTCGTCTTCTAGCGGGTGATGTTGGTTGTGGAAAAACTATTGTAGCACTTTTGGCTGCTCTAGAGGTTATCGATTCAGGGTATCAGGTAGCATTTATGGTGCCAACAGAATTATTAGCAATGCAGCATCATGGGAACATTATTTCTATTCTAGAGAGGATAAACAAAGATGACAGGCCTTCAGTAGCTCTTTTAACTGGTTCCACTCCATTAAAGGAAGCTCGTTTAATACAGAAGGGATTGGAGATGGGAGATATTGCATTGGTAGTAGGCACACACAAATTGTTTTCAGAAACTGTGAATTTTGCCTCTCTTTGCTTTTCAGTAATAGATGAACAACATCGCTTTGGAGTGGAACAAAAGGGAAGGTTTAGTAGCAAGGCTTGCACAGTTATGAACAAGTCAGTTGCAAATGGCAATAAGTTAGAAGATGGGCAGTTGGTTGCAAATAAGACATTCTTTGCTCCACATGTTCTTGTCATGTCTGCCACTCCAATTCCTAGGACCATGGCTTTAGCTCTTCATGGAAATATGTCACTCAGTAAGATAAATGAACTTCCACCAGGTCGAATTCCTGTGAAAACACTTGCACTCCATGGAAACAAAGTGGGGCTCACTAAAACATACCAGATGGTCCAGAAAGATCTTGACAAAGGAGGAAGAGTTTTTCTTGTTTACTCAGCAATAGATGAAAGTGAACAGCTTCCAGAACTTCATGCAGCTGCTGCAGAAATCAATAAAATATCTAATGTCTTTAAGGGTTACAAATGTGGTCTCGTTCATGGTCGTATGAATCATACTGAAAAAGAAGCAGCAATTCAACAGTTCAAAGAAGGAAAGACACAAGTTTTGCTCTCAACCCAGGTGGTTGAAGTTGGAATTGACATTCCTGAGGCAACAATGATGGTAATTATGAATGCTGAGAGGTTTGGAATAGCACAGTTGCATCAGCTCAGAGGTCGTGTTGGAAGGGGCACTGGAAAATCTACTTGTATACTTGTCTCATCCTCTGCAATTGGATTACCACGGCTGAAAATATTGCAGCAGTCAATGGATGGATTTGAATTGGCAGAAGCAGATCTCCGTCTTCGCGGCCCTGGAAGCTTGCTTGGCACGCGACAGTCAGGATACCTTCCAGAATTTTCTATTGCTCGGCTTCAGACTGACGGTGATGTTCTTGAGCAAGCTCATTTTGCAGCAGTGGACATTCTTCAACAGCACACTCGCTTGGACAGCCTGCCGATGCTCAAAGCCGAAATCAGTATGCGGTATCCTCTCAATTCACTTGGTGCATAGGCTTCCTAGGAGCGAGTTTGTAAATCTGTAGTTGCTTACAAAGTGAGATGACAACTATGAAGTAGTTTCTCTACATCATATTCCAATTGTAATGGTTTTTGGGAAAATCGTTTTGTAGCCCATGTACAGCTTTATACCGTGTTTAGAGCCATATTTTAAAGAAAATTGTTTATTGTTCATTATTCTTCAAGTAATGGAAAATGTCGTTTCAATATTCAAAGGGAAAATGAATGCAGGTCTTCTCCATATTCCTTGTTTAGGTTCTTTAAATTTGATATTCTAAGCTTAAACTTTTGGACCATGTATTCATGCTTTATGGAAAGTATCAGACATGCAGCAGATAAAGATTGCCGTCTATTTTA

Ace file of the UniGene sp_v3.0_unigene714

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	putative ATP-dependent DNA helicase RECG [Arabidopsis thaliana]	-	-	0.0	44%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	89%

Source	Gene names
Sma3	At2g01440; GSVIVT00030485001; OJ1038_A06.18; OJ1311_H06.28; Os02g0710800; OsI_08657; OsJ_08114; POPTRDRAFT_726887; RCOM_0410940; RCOM_0843050;

Source	GOs	Term	Type	Identity
Sma3	nucleic acid binding	GO:0003676	Molecular Function	-
Sma3	ATP-dependent DNA helicase activity	GO:0004003	Molecular Function	-
Sma3	helicase activity	GO:0004386	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	ATP-dependent helicase activity	GO:0008026	Molecular Function	-
Sma3	DNA repair	GO:0006281	Biological Process	-
Sma3	DNA recombination	GO:0006310	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Helicase, C-terminal	IPR001650	-	-
Sma3	DNA helicase, ATP-dependent, RecG	IPR004609	-	-
Sma3	DNA/RNA helicase, DEAD/DEAH box type, N-terminal	IPR011545	-	-
Sma3	Helicase, superfamily 1/2, ATP-binding domain	IPR014001	-	-
Sma3		IPR014021	-	-
Sma3	Peroxidases heam-ligand binding site	IPR019793	-	-

Source	Species	ID	Description	e value	Identity
RefSeq	Populus trichocarpa	XP_002314808.1	predicted protein [Populus trichocarpa]	0.0	48%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5AC88

Fln msg: your sequence is longer than subject: 1145 - 394

Fln protein: MLRVLLGASNSGRMYGICVSQHNSTLERRHILGGMWSVSLCKNGFVISQGTLPFSRCWVTLYGRTNNLQYQPAKCCYGFIQPSMPSMICQSNNLTVLSHTTIVSASNTESSTKVLQDKHLSSAIFYEAKNGFRDAQGKNMRFSNFLYSKLLRLCSPLVHKSAQSLLEEVKNYSRLCISDRLQILNKVSFLMGYGSVDDLIGHERALRECARPLQEGPSSSHCLPISSRHHADEVSGYPSSLDYSYVSEACLKFSSIHLGSSPQLDLYSAAEEKSEWVDCQLIKDVDKSTQPVDGTTFDSPNITETECIVRDQSNLFTACPVSNAEPNLWTLQLDKQDLHLSKQNRSETVQKEVLEGSATGNENMLLRKSTCKVMEATVPVNCFLDAPINTIKGIRPAQCRKLEENGFHTVRKLLQHFPRTYVNMQAAEGQVEDGQLLNFFGRILSSRGRRAGYSGGIVEVIIGCEIPSSGEYRTRSDEDYQDKGKRTVYIHLKRYFRGVRFTNTWILDKLQSKYNEGDLVSVSGKVKALEEADHFEIREYNIDVLKEEADLTRSEVCGKEEPCPIYPSKGDLDPRYIGECIRRVLEVLPAEMDPLPVNVCQKFNLMKLREAYFGIHCPKDLKEADHARRRLIFDDFFYHQLSLLYQRYGVVGTYIKTGGLPESSTGLEVGSIGVDKWSQLTLKVLKSLPFALTSAQLKAASEIIWDLRRPVPMSRLLAGDVGCGKTIVALLAALEVIDSGYQVAFMVPTELLAMQHHGNIISILERINKDDRPSVALLTGSTPLKEARLIQKGLEMGDIALVVGTHKLFSETVNFASLCFSVIDEQHRFGVEQKGRFSSKACTVMNKSVANGNKLEDGQLVANKTFFAPHVLVMSATPIPRTMALALHGNMSLSKINELPPGRIPVKTLALHGNKVGLTKTYQMVQKDLDKGGRVFLVYSAIDESEQLPELHAAAAEINKISNVFKGYKCGLVHGRMNHTEKEAAIQQFKEGKTQVLLSTQVVEVGIDIPEATMMVIMNAERFGIAQLHQLRGRVGRGTGKSTCILVSSSAIGLPRLKILQQSMDGFELAEADLRLRGPGSLLGTRQSGYLPEFSIARLQTDGDVLEQAHFAAVDILQQHTRLDSLPMLKAEISMRYPLNSLGA*

Protein Length: 1146

Fln nts: TCCTTCAACTGCTTTCCTCGTGGTTTGCAACGGCACTGGTTTGGATCTGGCTACCGCTTGAAGGATTATCACCTCTTTTCCACAAAATTTGCACTTTACGCAACCTTTTATTGTATTGAAGATCGGGTTCAGAATAAGGGTCATTCCTTACTTGCAGGGCACAGTACCTTCCGAGGAGATACTGCTACTTATTTTTTAGTACAGATTCCTCTTACTCTGGAGAACCTGGATGCAAATAAGTTTCAGACTATGCTTTTGAAACCTAAGCATAAAGGTGAAAAGCAAGCTAGAAGGCAGCTGTGATGAAACCGAAGGGCTGAACTTGCACAAG_-_ATGCTACGGGTGCTTTTGGGTGCATCTAATTCTGGAAGGATGTATGGGATATGTGTTTCCCAACATAATTCTACTTTGGAAAGAAGGCATATTCTTGGAGGAATGTGGTCTGTGTCATTATGTAAAAATGGATTTGTGATTAGTCAAGGCACTTTACCATTTTCAAGGTGCTGGGTGACTTTGTATGGGAGGACAAATAATCTCCAATATCAGCCTGCTAAATGTTGCTATGGTTTTATACAACCGAGCATGCCTAGCATGATTTGTCAATCAAACAATCTTACAGTGCTTTCTCACACCACTATAGTTTCAGCTTCGAATACTGAATCAAGTACTAAGGTTCTTCAGGATAAACATTTGTCAAGTGCAATCTTTTATGAGGCAAAAAATGGTTTTAGAGATGCTCAAGGGAAGAATATGAGGTTCAGTAACTTTCTATATTCAAAGCTTTTACGGTTATGTTCTCCTTTAGTTCATAAGTCTGCTCAAAGTTTATTAGAGGAAGTGAAGAACTACTCTCGATTGTGCATATCAGATCGTTTGCAGATTCTTAACAAGGTGTCCTTCCTTATGGGATATGGCAGTGTAGATGATTTGATTGGCCATGAAAGGGCTTTAAGGGAGTGTGCACGCCCGCTTCAAGAAGGGCCAAGTTCTTCACATTGCTTGCCAATTAGTTCTAGACATCATGCAGATGAAGTAAGTGGGTATCCATCTTCACTGGATTACTCTTATGTTTCAGAAGCTTGTCTGAAATTCTCGTCTATTCATTTGGGAAGCAGCCCACAACTGGATCTATATAGTGCAGCTGAGGAGAAGTCAGAATGGGTTGATTGTCAACTCATTAAGGACGTGGACAAATCTACTCAGCCAGTAGATGGCACTACTTTCGATTCACCAAATATCACAGAAACTGAATGTATAGTTAGGGACCAATCTAATCTTTTCACTGCTTGCCCGGTATCAAATGCTGAGCCAAATCTGTGGACACTCCAGTTAGATAAGCAAGACTTGCATTTATCAAAACAGAACAGGTCAGAAACTGTACAGAAGGAAGTTTTAGAAGGCAGTGCCACGGGCAATGAAAATATGTTACTGAGGAAGTCTACATGTAAAGTAATGGAAGCAACTGTTCCTGTCAATTGCTTCCTTGATGCACCTATTAACACTATAAAGGGAATACGACCAGCACAATGCCGTAAACTGGAAGAGAATGGATTTCACACGGTGCGTAAACTACTTCAACATTTCCCTCGTACTTATGTGAATATGCAAGCTGCTGAAGGCCAAGTAGAGGATGGGCAGTTGCTGAATTTCTTTGGAAGGATTTTATCTTCTAGAGGACGAAGGGCAGGGTATAGTGGTGGGATTGTTGAAGTCATTATTGGGTGTGAAATCCCTAGCAGTGGTGAATATCGTACTAGAAGTGATGAAGATTATCAGGATAAGGGAAAAAGGACTGTTTATATTCATTTGAAGAGATATTTCAGAGGAGTACGATTCACAAATACTTGGATTCTTGATAAATTGCAGTCGAAATATAATGAAGGTGATCTAGTATCAGTGAGTGGTAAGGTCAAGGCTTTAGAAGAAGCTGATCATTTTGAAATTCGGGAATATAACATTGATGTGCTGAAAGAGGAGGCAGATCTTACAAGGTCAGAAGTATGTGGAAAAGAGGAGCCTTGTCCTATTTATCCTTCAAAAGGTGATTTGGATCCAAGATACATAGGGGAATGCATTAGGAGAGTATTAGAAGTTTTACCTGCAGAGATGGATCCTCTTCCGGTCAATGTTTGTCAAAAGTTCAACTTGATGAAGCTTCGTGAAGCTTACTTTGGCATCCATTGTCCCAAGGATTTAAAGGAAGCTGACCATGCGCGCAGGAGGTTGATCTTTGATGACTTCTTTTATCATCAGTTGAGTTTATTGTATCAAAGGTATGGAGTGGTAGGCACATATATCAAAACTGGTGGTTTGCCCGAGAGTTCAACAGGGCTCGAAGTTGGAAGTATTGGTGTAGATAAATGGTCACAACTGACTTTGAAAGTGCTGAAATCACTTCCTTTTGCACTTACCAGTGCTCAGTTGAAAGCTGCATCAGAAATTATTTGGGACCTCAGAAGGCCAGTACCAATGAGTCGTCTTCTAGCGGGTGATGTTGGTTGTGGAAAAACTATTGTAGCACTTTTGGCTGCTCTAGAGGTTATCGATTCAGGGTATCAGGTAGCATTTATGGTGCCAACAGAATTATTAGCAATGCAGCATCATGGGAACATTATTTCTATTCTAGAGAGGATAAACAAAGATGACAGGCCTTCAGTAGCTCTTTTAACTGGTTCCACTCCATTAAAGGAAGCTCGTTTAATACAGAAGGGATTGGAGATGGGAGATATTGCATTGGTAGTAGGCACACACAAATTGTTTTCAGAAACTGTGAATTTTGCCTCTCTTTGCTTTTCAGTAATAGATGAACAACATCGCTTTGGAGTGGAACAAAAGGGAAGGTTTAGTAGCAAGGCTTGCACAGTTATGAACAAGTCAGTTGCAAATGGCAATAAGTTAGAAGATGGGCAGTTGGTTGCAAATAAGACATTCTTTGCTCCACATGTTCTTGTCATGTCTGCCACTCCAATTCCTAGGACCATGGCTTTAGCTCTTCATGGAAATATGTCACTCAGTAAGATAAATGAACTTCCACCAGGTCGAATTCCTGTGAAAACACTTGCACTCCATGGAAACAAAGTGGGGCTCACTAAAACATACCAGATGGTCCAGAAAGATCTTGACAAAGGAGGAAGAGTTTTTCTTGTTTACTCAGCAATAGATGAAAGTGAACAGCTTCCAGAACTTCATGCAGCTGCTGCAGAAATCAATAAAATATCTAATGTCTTTAAGGGTTACAAATGTGGTCTCGTTCATGGTCGTATGAATCATACTGAAAAAGAAGCAGCAATTCAACAGTTCAAAGAAGGAAAGACACAAGTTTTGCTCTCAACCCAGGTGGTTGAAGTTGGAATTGACATTCCTGAGGCAACAATGATGGTAATTATGAATGCTGAGAGGTTTGGAATAGCACAGTTGCATCAGCTCAGAGGTCGTGTTGGAAGGGGCACTGGAAAATCTACTTGTATACTTGTCTCATCCTCTGCAATTGGATTACCACGGCTGAAAATATTGCAGCAGTCAATGGATGGATTTGAATTGGCAGAAGCAGATCTCCGTCTTCGCGGCCCTGGAAGCTTGCTTGGCACGCGACAGTCAGGATACCTTCCAGAATTTTCTATTGCTCGGCTTCAGACTGACGGTGATGTTCTTGAGCAAGCTCATTTTGCAGCAGTGGACATTCTTCAACAGCACACTCGCTTGGACAGCCTGCCGATGCTCAAAGCCGAAATCAGTATGCGGTATCCTCTCAATTCACTTGGTGCATAG___GCTTCCTAGGAGCGAGTTTGTAAATCTGTAGTTGCTTACAAAGTGAGATGACAACTATGAAGTAGTTTCTCTACATCATATTCCAATTGTAATGGTTTTTGGGAAAATCGTTTTGTAGCCCATGTACAGCTTTATACCGTGTTTAGAGCCATATTTTAAAGAAAATTGTTTATTGTTCATTATTCTTCAAGTAATGGAAAATGTCGTTTCAATATTCAAAGGGAAAATGAATGCAGGTCTTCTCCATATTCCTTGTTTAGGTTCTTTAAATTTGATATTCTAAGCTTAAACTTTTGGACCATGTATTCATGCTTTATGGAAAGTATCAGACATGCAGCAGATAAAGATTGCCGTCTATTTTA

Fln Alignment:

Contig714___MVPTELLAMQHHGNIISILERINKDDRPSVALLTGSTPLKEARLIQKGLEMGDIALVVGTHKLFSETVNFASLCFSVIDEQHRFGVEQKGRFSSKACTVMNKSVANGNKLEDGQLVANKTFFAPHVLVMSATPIPRTMALALHGNMSLSKINELPPGRIPVKTLALHGNKVGLTKTYQMVQKDLDKGGRVFLVYSAIDESEQLPELHAAAAEINKISNVFKGYKCGLVHGRMNHTEKEAAIQQFKEGKTQVLLSTQVVEVGIDIPEATMMVIMNAERFGIAQLHQLRGRVGRGTGKSTCILVSSSAIGLPRLKILQQSMDGFELAEADLRLRGPGSLLGTRQSGYLPEFSIARLQTDGDVLEQAHFAAVDILQQHTRLDSLPMLKAEISMRYPLNSLGA
D5AC88_____________MVPTELLATQHYENIISMLERINKDDRPSVAFLTGSTPLKETRLIRKGLEMGDIALVVGTHKLFSESVNFASLCFAVIDEQHRFGVEQKGRFSSKACTVMDKSGANGIKLEDGQLVANKTFFAPHVLVMSATPIPRTLALTLHGNMSLSKINELPPGRIPVKTLALHGNTKGLTKTYQMVQKELDSGGRVFLVYPAIDESEQLPELHAAAAEIKKISKVFKGYKCGLLHGRMKTTEKEKAIQQFKEGKTQILLSTQVVEVGIDIPEATMMVIMNAERFGISQLHQFRGRVGRGTGKSTCILVSSSAVGLPRLKILQQSMDGFELAEADLHLRGPGSLLGTRQSGHLPEFSIARLQTDGDVLEKAHFAAL-----HPCLDSLPMLKAEISMRHPLNSLGA

SNPs (tot: 11)

Position	A %	C %	G %	T %	Probability
2542		25	25	50	0.995533
2829	83		16		0.994512
2905	16		83		0.994512
3269	60			40	1.0
3279	83			16	0.994512
3281	83			16	0.994512
3434	16		83		0.994512
3437			16	83	0.994512
3564		83		16	0.994512
3780	12		87		0.992554
3832	75	25			1.0

Back

Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain