SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene270

Length: 1953 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v3.0_unigene270
GTCATGTGTCTACACGGCTAGACCACGGCCAGAAGAATCGTCGTGACCTTGGCTGTCACTCAATTTTTTTACTTTGTCATCGCACGTGCTCTTCAAGAATTTATTTTGCAATCGATCGCCTGTGCCTGGAGACGATTCTGGTTTCTTCGTCAACTGAGGCTAGCGAATCGTGGTTGAGGGATGCGATCGAAAACGACTTCATTGGCATTTGACGTGCATGCAGAGATTCCGAATTCTCTATAGCAATGGAACACCGTCGTCACCACCATTATCACCAGAACGAGGAAATTGTGTCTGGAAACGCGATGGAGCCCGCAACATCTAAGGTAGATCTAGATTTGAAAACCGAAAAGCTAGTTTCTCCTCGTTATTCGGCAATAGGAAGCAAATCATTTGGCTATCGAAACCCCGAGCAAGAAGAAAATTTGGACCGCAGGTTTGCATTCAGCCGTCAAAGCTCTTTCAAACAAAACCCAACCCAGCCTTCAATTTCTATCATCCCTAATGAAACCCTAAAACCTTCGCTATCCCGAAGCTTATCCAGCATATCCCGTGATCACTATCATAACCAACACCATCACCATCATAACCACCATCTATTTAGCAATTCTCAATTGTCGGACAAGACAACGCCTTTGTTAGAAGATGGACAGTTGGGGAAAGGTGTTCTGGTCAGTGCTGAGACTAGTGGTAAGAAGCCCAATTTTAACAGCTATGGCTCTTTCCGGCATTCGGACTCCTTGTTCGGACTTGTGTGGACGGCGATTCCGATCATCAGCTCTGGAAGCCGTCCGATGAGGAGACTTTTTACTTTAATTATGCTTAACGTTGCTTATTCGACCTTGGAACTGCTTATTGGACTCTTCACCGGGCGTGTAGGTTTGGTATCAGATGCATTTCATTTAACATTTGGTTGTGGTGTGCTATCCTTTTCCTTATTTGCAATGGCAGTTGCCAGGAAGAAACCTGACGATGTGTATTCCTATGGGTACAAGCGAGTAGAGGTTCTATCAGCTTTTACAAATGCTCTATTTCTTTTGTTTCTTTCATTTTCCCTTGCCGTGGAAGCATTGCATGCATTCATGCAGGATGAATCAGAGCACAAGCATTATTTGATTGTATCTGCTGTTGCAAACCTTTTGGTGAACCTTCTGGGTGTCTGGTTTTTCCGAGGGTATGCACGTGTTTATATTGTGTATAGAAAAGCTGAAGACATGAATTACCATTCGGTTTGCTTGCATGTGCTCTCTGATTCCATTCGAAGTGCAGGATTGATCCTAGCATCATGGCTTTTATCTCTTGGGATAAAGAATGCAGAAATGTTATGTCTCGGTTTGGTATCCATTGCTATTTTCATTGTGGTAATGCCACTCTTTAAAGAAGCCGCGGGCATTTTGCTACAAACAGCACCATCTGGCATATCTTCATCAGCGTTCAACAAATGCCTTAGACAGATTAATATTCTCGAGGATGTATCTGAAGTTTCACAGGCTCGATTCTGGGAAATGGTACCAGGCTTTGTAGTAGGTTCACTGTCATTGCAGTTGAAGAAAGGGTCCGATGAGTATAAAATTTTGAAGCATGTGCATAGTTCATACAATGATATTGGAGTAAAGGATTTGACTGTCCAGACGGATTTTGTATAAGTATTGTATTGCAAAGTCTCCATCTAAGAAATTAGCAGGGAGCATATGCATTACAGCAGGATGCTGACATGTTGATAGACTATAGCTGGTCTTGGGTAACTTCTTTAAAGGTCCCGTTTATCATTTTTTTGTGTTTCCATCGATTGGCATAACAATTATATGATATATCACTTAAAGAACGTTTTTGTTAACAAGAAAAGTGTGTTACATGTAATTTTTTTTGGGGAAAATATTCACTGATTTGATTGCATAGTTGCGGACAGTTCTTTGATATGAAATTCTAATGTGTTCACTGTTCCCTGACTTG

Ace file of the UniGene sp_v3.0_unigene270 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	[P] COG1230 Co/Zn/Cd efflux system component	-	-	7.0e-13	25%
FL-Next	sp=Metal tolerance protein C2; Arabidopsis thaliana (Mouse-ear cress).	-	-	0.0	74%
Sma3	Cation efflux family protein-like	-	-	1.577e-09	-

Source	Gene names
Sma3	AT3G12100; At3g12100; GSVIVT00025388001; MTPC2; OJ1149_C12.26-1; OJ1149_C12.26-2; OJ1282_E10.2-1; OJ1282_E10.2-2; Os02g0832700; OsI_09578; OsJ_09007; PHYPADRAFT_204337; POPTRDRAFT_667366; PtrMTP5; RCOM_0503980; T21B14.8; VITISV_009633;

Source	GOs	Term	Type	Identity
Sma3	vacuolar membrane	GO:0005774	Cellular Component	-
Sma3	membrane	GO:0016020	Cellular Component	-
Sma3	integral to membrane	GO:0016021	Cellular Component	-
Sma3	cation transmembrane transporter activity	GO:0008324	Molecular Function	-
Sma3	hydrolase activity	GO:0016787	Molecular Function	-
Sma3	metal ion binding	GO:0046872	Molecular Function	-
Sma3	cation transport	GO:0006812	Biological Process	-
Sma3	response to nematode	GO:0009624	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	NUDIX hydrolase domain	IPR000086	-	-
Sma3	Cation efflux protein	IPR002524	-	-
Sma3	EGF-like region, conserved site	IPR013032	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT3G12100.1	Cation efflux family protein chr3:3854741-3857270 REVERSE LENGTH=393	74%
RefSeq	Arabidopsis thaliana	NP_187817.2	metal tolerance protein C2 [Arabidopsis thaliana]	74%
RefSeq	Populus trichocarpa	XP_002323276.1	metal tolerance protein [Populus trichocarpa]	71%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: sp_plants

Fln subject: Q6ICY4

Fln msg: your sequence is longer than subject: 467 - 393

Fln protein: MEHRRHHHYHQNEEIVSGNAMEPATSKVDLDLKTEKLVSPRYSAIGSKSFGYRNPEQEENLDRRFAFSRQSSFKQNPTQPSISIIPNETLKPSLSRSLSSISRDHYHNQHHHHHNHHLFSNSQLSDKTTPLLEDGQLGKGVLVSAETSGKKPNFNSYGSFRHSDSLFGLVWTAIPIISSGSRPMRRLFTLIMLNVAYSTLELLIGLFTGRVGLVSDAFHLTFGCGVLSFSLFAMAVARKKPDDVYSYGYKRVEVLSAFTNALFLLFLSFSLAVEALHAFMQDESEHKHYLIVSAVANLLVNLLGVWFFRGYARVYIVYRKAEDMNYHSVCLHVLSDSIRSAGLILASWLLSLGIKNAEMLCLGLVSIAIFIVVMPLFKEAAGILLQTAPSGISSSAFNKCLRQINILEDVSEVSQARFWEMVPGFVVGSLSLQLKKGSDEYKILKHVHSSYNDIGVKDLTVQTDFV*

Protein Length: 468

Fln nts: GTCATGTGTCTACACGGCTAGACCACGGCCAGAAGAATCGTCGTGACCTTGGCTGTCACTCAATTTTTTTACTTTGTCATCGCACGTGCTCTTCAAGAATTTATTTTGCAATCGATCGCCTGTGCCTGGAGACGATTCTGGTTTCTTCGTCAACTGAGGCTAGCGAATCGTGGTTGAGGGATGCGATCGAAAACGACTTCATTGGCATTTGACGTGCATGCAGAGATTCCGAATTCTCTATAGCA_-_ATGGAACACCGTCGTCACCACCATTATCACCAGAACGAGGAAATTGTGTCTGGAAACGCGATGGAGCCCGCAACATCTAAGGTAGATCTAGATTTGAAAACCGAAAAGCTAGTTTCTCCTCGTTATTCGGCAATAGGAAGCAAATCATTTGGCTATCGAAACCCCGAGCAAGAAGAAAATTTGGACCGCAGGTTTGCATTCAGCCGTCAAAGCTCTTTCAAACAAAACCCAACCCAGCCTTCAATTTCTATCATCCCTAATGAAACCCTAAAACCTTCGCTATCCCGAAGCTTATCCAGCATATCCCGTGATCACTATCATAACCAACACCATCACCATCATAACCACCATCTATTTAGCAATTCTCAATTGTCGGACAAGACAACGCCTTTGTTAGAAGATGGACAGTTGGGGAAAGGTGTTCTGGTCAGTGCTGAGACTAGTGGTAAGAAGCCCAATTTTAACAGCTATGGCTCTTTCCGGCATTCGGACTCCTTGTTCGGACTTGTGTGGACGGCGATTCCGATCATCAGCTCTGGAAGCCGTCCGATGAGGAGACTTTTTACTTTAATTATGCTTAACGTTGCTTATTCGACCTTGGAACTGCTTATTGGACTCTTCACCGGGCGTGTAGGTTTGGTATCAGATGCATTTCATTTAACATTTGGTTGTGGTGTGCTATCCTTTTCCTTATTTGCAATGGCAGTTGCCAGGAAGAAACCTGACGATGTGTATTCCTATGGGTACAAGCGAGTAGAGGTTCTATCAGCTTTTACAAATGCTCTATTTCTTTTGTTTCTTTCATTTTCCCTTGCCGTGGAAGCATTGCATGCATTCATGCAGGATGAATCAGAGCACAAGCATTATTTGATTGTATCTGCTGTTGCAAACCTTTTGGTGAACCTTCTGGGTGTCTGGTTTTTCCGAGGGTATGCACGTGTTTATATTGTGTATAGAAAAGCTGAAGACATGAATTACCATTCGGTTTGCTTGCATGTGCTCTCTGATTCCATTCGAAGTGCAGGATTGATCCTAGCATCATGGCTTTTATCTCTTGGGATAAAGAATGCAGAAATGTTATGTCTCGGTTTGGTATCCATTGCTATTTTCATTGTGGTAATGCCACTCTTTAAAGAAGCCGCGGGCATTTTGCTACAAACAGCACCATCTGGCATATCTTCATCAGCGTTCAACAAATGCCTTAGACAGATTAATATTCTCGAGGATGTATCTGAAGTTTCACAGGCTCGATTCTGGGAAATGGTACCAGGCTTTGTAGTAGGTTCACTGTCATTGCAGTTGAAGAAAGGGTCCGATGAGTATAAAATTTTGAAGCATGTGCATAGTTCATACAATGATATTGGAGTAAAGGATTTGACTGTCCAGACGGATTTTGTATAA___GTATTGTATTGCAAAGTCTCCATCTAAGAAATTAGCAGGGAGCATATGCATTACAGCAGGATGCTGACATGTTGATAGACTATAGCTGGTCTTGGGTAACTTCTTTAAAGGTCCCGTTTATCATTTTTTTGTGTTTCCATCGATTGGCATAACAATTATATGATATATCACTTAAAGAACGTTTTTGTTAACAAGAAAAGTGTGTTACATGTAATTTTTTTTGGGGAAAATATTCACTGATTTGATTGCATAGTTGCGGACAGTTCTTTGATATGAAATTCTAATGTGTTCACTGTTCCCTGACTTG

Fln Alignment:

Contig270___SLFGLVWTAIPIISSGSRPMRRLFTLIMLNVAYSTLELLIGLFTGRVGLVSDAFHLTFGCGVLSFSLFAMAVARKKPDDVYSYGYKRVEVXXXXXXXXXXXXXXXXXXXXXLHAFMQDESEHKHYLIVSXXXXXXXXXLGVWFFRGYARVYIVYRKAEDMNYHSVCLHVLSDSIRSAGLILASWLLSLGIKNAEMLCLGLVSIAIFIVVMPLFKEAAGILLQTAPSGISSSAFNKCLRQINILEDVSEVSQARFWEMVPGFVVGSLSLQLKKGSDEYKILKHVHSSYNDIGVKDLTVQTDF
Q6ICY4_____________SVLEMVWEVFGVVTSGNRQMKRLFLLIALNVLYSTTELSIGIFTGRVGLVSDAFHLTFGCGLLTFSLFAMATSRKKPDHAYSYGYKRLEVLSAFTNALFLMFMSFSLAVEALHAFIQDESEHKHYLIVSAVTNLLVNLLGVWFFRNYARVNIAYRKAEDMNYHSVCLHVISDSIRSAGLILASWLLSLGVENAEVLCLGLVSVTVFMLVMPLFKATGGVLLQMAPPNIPSSALSKCLRQITSREDVTEVLQARFWEVVPGHTVGSLRLQVKSGIDERPLLQYVYDVYHDLGVQDLTLQTDY

SNPs (tot: 43)

Position	A %	C %	G %	T %	Probability
1231	14		85		0.993533
1479		20	80		0.995493
1520		66		33	1.0
1612			50	50	1.0
1656	15		84		0.999986
1855	75			25	1.0
1857	75		25		1.0
1859	75		25		1.0
1862	25			75	1.0
1865			25	75	1.0
1866		25		75	1.0
1867		25	75		1.0
1871	75		25		1.0
1872			75	25	1.0
1874	87	12			0.992554
1875		87		12	0.992554
1877	12			62	1.0
1878	28		71		1.0
1879		85		14	0.993533
1883	20			80	0.995493
1884		20	80		0.995493
1885	60	40			1.0
1888	80			20	0.995493
1889	75		25		0.996473
1892	50	25			0.995533
1894	75	25			0.996473
1895	75		25		0.996473
1897	75	25			0.996473
1898			25	25	0.995533
1901	33			66	0.997452
1902	33		66		0.997452
1903	66			33	0.997452
1904	33		66		0.997452
1907	66			33	0.997452
1908		66		33	0.997452
1909	66			33	0.997452
1910			66	33	0.997452
1911	33	66			0.997452
1912		66	33		0.997452
1919	66		33		0.997452
1921			66	33	0.997452
1923		66		33	0.997452
1924	33		66		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain