SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene333

Length: 1675 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene333

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	tropine dehydrogenase [Arabidopsis thaliana] dbj\|BAA98195.1\| short chain alcohol dehydrogenase-like [Arabidopsis thaliana] gb\|AAO22710.1\| putative short chain alcohol dehydrogenase [Arabidopsis thaliana] gb\|AAO42448.1\| putative short chain alcohol dehydro	-	-	70%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	94%
Sma3	Putative tropinone reductase	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3	Tropinone reductase I.	EC:1.1.1.206	-	2.788e-32	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Tropane, piperidine and pyridine alkaloid biosynthesis	00960		2.788e-32	%
Sma3	Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid	01064		2.788e-32	%
Sma3	Metabolic pathways	01100		2.788e-32	%
Sma3	Biosynthesis of secondary metabolites	01110		2.788e-32	%
Sma3	Tropinone reductase II.	EC:1.1.1.236	-	4.915e-07	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Tropane, piperidine and pyridine alkaloid biosynthesis	00960		4.915e-07	%

Source	Gene names
Sma3	At1g07440; At1g07450; At2g29150; At2g29170; At2g29260; At2g29290; At2g29300; At2g29310; At2g29320; At2g29330; At2g29340; At2g29350; At2g29350/F16P2.27; At2g29360; At2g29370; At2g30670; At5g06060; CHLREDRAFT_128624; DcADH; F22G5.20; F22G5.39; F22G5_16; GSV

Source	GOs	Term	Type	Identity
Sma3	intracellular	GO:0005622	Cellular Component	-
Sma3	DNA binding	GO:0003677	Molecular Function	-
Sma3	alcohol dehydrogenase (NAD) activity	GO:0004022	Molecular Function	-
Sma3	binding	GO:0005488	Molecular Function	-
Sma3	zinc ion binding	GO:0008270	Molecular Function	-
Sma3	oxidoreductase activity	GO:0016491	Molecular Function	-
Sma3	tropine dehydrogenase activity	GO:0050356	Molecular Function	-
Sma3	tropinone reductase activity	GO:0050358	Molecular Function	-
Sma3	metabolic process	GO:0008152	Biological Process	-
Sma3	DNA integration	GO:0015074	Biological Process	-
Sma3	oxidation-reduction process	GO:0055114	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Integrase, catalytic core	IPR001584	-	-
Sma3	Zinc finger, CCHC-type	IPR001878	-	-
Sma3	Short-chain dehydrogenase/reductase SDR	IPR002198	-	-
Sma3	Glucose/ribitol dehydrogenase	IPR002347	-	-
Sma3	Sugar transporter, conserved site	IPR005829	-	-
Sma3	Acyl-CoA dehydrogenase, conserved site	IPR006089	-	-
Sma3	Zinc finger, C2H2	IPR007087	-	-
Sma3	Reverse transcriptase, RNA-dependent DNA polymerase	IPR013103	-	-
Sma3	Zinc finger, C2H2-like	IPR015880	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G06060.1	NAD(P)-binding Rossmann-fold superfamily protein chr5:1824066-1825833 REVERSE LENGTH=264	70%
RefSeq	Arabidopsis thaliana	NP_196225.1	tropine dehydrogenase [Arabidopsis thaliana]	70%
RefSeq	Populus trichocarpa	XP_002311182.1	predicted protein [Populus trichocarpa]	69%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5AD75

Fln msg:

Fln protein: MGESSSSRQQRWSLQGMTALVTGGTKGIGRAVVEELTSLGAAVYTCARTEKDLNECLTQWKESGLQVDGTVCDLSSRSAREELIEKCSSFCNGKLNMLINNVGTNMRKPTVGYTAEDFSFLMSTNFESGYHLCQLSHPLLKASEKGSIVFISSVAGVVAIFSGTIYAASKGAMNQLTKNLACEWASDNIRVNCVAPWYTKTSLVEKLLEDKEFVENILDRTPLKRLAEPHEVSSLVAFLCLPAASYITGQVISVDGGMTVNGFYI*

Protein Length: 267

Fln nts: ATAAATAGAGGAATAGAGAACGTAAGAGACTAACACGAAACCCGTGTTCTCCACCCAGACATTTATTTATCCATTTCTGTAAAACCATAGTGTGCTATGCATATATTTTGTATAGATCACGAAGTTTCAATTATTCTGCTGATGAATCATCATCGATTGGAGGCTTCAGTATTTGTTTCTCTTGCAATACCACTCCTTCAGTTAAGTTTAGATTTTTTCCGCTGGTCTTGAATTTGTCTGTTGCTGCGCGCCCATGAGATAATATATAGGATCCCGAGAACTCATTTCATCAAGTGTTTTGCAAAGTTGGGTTGTATTTTTTCATTGCACATAGAGTAGG_-_ATGGGGGAGAGTTCAAGTAGCAGACAACAGAGGTGGTCGCTGCAAGGCATGACAGCCCTAGTCACTGGCGGTACAAAAGGAATTGGACGTGCTGTTGTGGAGGAATTAACTAGTTTAGGAGCTGCTGTTTATACATGTGCGCGTACAGAGAAGGATCTTAATGAATGTTTGACTCAGTGGAAGGAATCCGGGCTGCAGGTTGATGGTACAGTCTGTGATTTATCATCTCGTTCAGCACGAGAGGAGCTGATTGAAAAATGTTCTTCATTTTGCAATGGGAAACTCAATATGTTGATTAATAATGTCGGTACAAATATGAGGAAGCCCACTGTGGGTTATACAGCTGAGGATTTCTCATTTCTTATGTCTACCAACTTTGAATCAGGTTATCATCTATGTCAGCTGTCACATCCTCTACTAAAAGCATCAGAAAAGGGAAGCATTGTTTTCATTTCATCTGTTGCTGGTGTTGTAGCTATTTTCTCTGGTACCATATATGCAGCCAGCAAAGGAGCAATGAACCAACTCACAAAGAATCTTGCATGTGAGTGGGCAAGTGACAATATACGTGTTAACTGTGTGGCACCATGGTATACCAAGACATCACTCGTGGAAAAGCTCTTGGAAGACAAAGAGTTTGTAGAAAATATACTTGATCGTACTCCTTTAAAGCGTCTTGCTGAGCCTCATGAAGTTTCATCCCTCGTAGCTTTTCTTTGCCTACCTGCAGCTTCATATATTACTGGTCAGGTTATTTCTGTTGATGGAGGAATGACAGTAAACGGTTTCTATATATGA___GATCAGAAAGTAAGGCTTAGTGTTGTGTTTCTCTAAAATACCAAGGTAAGGTTTGATGTGATCTCAAAGTATCCCTATGTTGATATTTCATTAAGTATTAACACTCTTTTTCATCTAAACTTTGTAATAAATGTTTAATTAATTTGTATAACCTCTGACCATTTATGATAACCTGATTTGATTACATTAGAGACGTAATAGTTGCATCTCCATAGAGTTCCCGGTCATGCATTGGTTTATGCTTTGTTTGAGAAACAAATTGCAAGGTAAACTTTGCATGATGTTTTAAACCCTTCATGGTCTGAATCATGGCATGCTTGGTGGCTGAATTTCTCATGCAGTCAGTTACTTCTGAATATTGATGTTACTATATGTTTCTGTTAACATATGTTTCCTTCTACATTTGAAGTGTTATATATTGTCACTGATATATCCTACACAAGACACTCAGAGTTTGGTCTACATACCCTCTATCCGTTTAAGAGTTATTGTAATTATGTTTATTTAGATGCTATGTGTTTTCAGTAGCATGACTAT

Fln Alignment:

Contig333___MGEXXXXXXXXXXLQGMTALVTGGTKGIGRAVVEELTSLGAAVYTCARTEKDLNECLTQWKESGLQVDGTVCDLSSRSAREELIEKCSSFCNGKLNMLINNVGTNMRKPTVGYTAEDFSFLMSTNFESGYHLCQLSHPLLKASEKGSIVFISSVAGVVAIFSGTIYAASKGAMNQLTKNLACEWASDNIRVNCVAPWYTKTSLVEKLLEDKEFVENILDRTPLKRLAEPHEVSSLVAFLCLPAASYITGQVISVDGGMTVNGFYI
D5AD75_____________MGESSSSRQQRWSLQGMTALVTGGTKGIGRAVVEELTGLGAAVYTCARTEKDLNECLTQWKEAGLHVGGSVCDLSSRSAREELVEKSSSFCNGKLNMLINNVGTNMRKPTVGYTAEDFSFLMSTNFESAYHLCQLSHPLLKASGKGSIVFISSVAGVVAIFSGTLYAASKGAMNQITKNLACEWASDKIRVNCVAPWYTKTSLVEKLLEDKEFVEKILDRTPLRRLAEPHEVSSLVAFLCLPAASYITGQIISVDGGMTVNGFYI

SNPs (tot: 16)

Position	A %	C %	G %	T %	Probability
49	33	66			0.997452
52		66	33		0.997452
53		66	33		0.997452
316		80		20	0.995493
482			33	66	0.997452
845		50	50		1.0
913		10		90	0.90298
939	90	10			0.90298
1204		14		85	0.999981
1290	85	14			0.993533
1298		85	14		0.993533
1344		75		25	0.996473
1360		75		25	0.996473
1411		66	33		0.997452
1464		60		40	1.0
1519			25	75	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain